| 质粒名称: | pBT100.164 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 138522 |
| 存储实验室: | David Liu |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV with a BR322 origin |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | Synthetic Biology |
| 筛选标记: | -- |
| 载体抗性: | 卡那霉素50 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | -- |
|
插入基因信息 |
|
| 插入基因名称: | ecTadA(7.10)-inteinN |
| 物种来源: | Other |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pBT100.164 was a gift from David Liu (Addgene plasmid # 138522 ; http://n2t.net/addgene:138522 ; RRID:Addgene_138522) |
| 参考文献部分: | Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity. Richter MF, Zhao KT, Eton E, Lapinaite A, Newby GA, Thuronyi BW, Wilson C, Koblan LW, Zeng J, Bauer DE, Doudna JA and Liu DR. Nat Biotechnol (2020) 10.1038/s41587-020-0453-z |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBT100.164 2775 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION phage cloning.
ACCESSION .
VERSION .
KEYWORDS pBT100.164
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2775)
AUTHORS Richter MF, Zhao KT, Eton E, Lapinaite A, Newby GA, Thuronyi BW,
Wilson C, Koblan LW, Zeng J, Bauer DE, Doudna JA, Liu DR
TITLE Phage-assisted evolution of an adenine base editor with improved Cas
domain compatibility and activity
JOURNAL Nat Biotechnol (2020)
REFERENCE 2 (bases 1 to 2775)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..2775
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 227..244
/label=L4440
/note="L4440 vector, forward primer"
CDS 276..770
/codon_start=1
/product="DNA adenosine deaminase evolved from the E. coli
tRNA adenosine deaminase TadA (Gaudelli et al., 2017)"
/label=TadA*(7.10)
/translation="EVEFSHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGWNR
AIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTFEPCVMCAGAMIHSRIGRVVFGV
RNAKTGAAGSLMDVLHYPGMNHRVEITEGILADECAALLCYFFRMPRQVFNAQKKAQSS
TD"
CDS complement(1323..2138)
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind 2046..2065
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
primer_bind 2130..2149
/label=pENTR-R
/note="pENTR vectors, reverse primer"
rep_origin 2260..73
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2749..2768
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
61 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
121 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
181 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
241 cgagaatgct cttctagtaa ggaggaaaaa tgtcagaagt agagttttca cacgagtact
301 ggatgagaca cgcattgact ctcgcaaagc gtgctcgaga tgaacgcgag gtgcccgtgg
361 gagcagtact cgtgctcaac aatcgcgtaa tcggcgaagg ttggaatcgt gcaatcggac
421 tccacgaccc cactgcacat gcggaaatca tggcccttcg acagggcggg cttgtgatgc
481 agaattatcg acttatcgat gcgacgctgt acgtcacgtt tgaaccttgc gtaatgtgcg
541 cgggagctat gattcactcc cgcattggac gagttgtatt cggtgttcgc aacgccaaga
601 cgggtgccgc aggttcactg atggacgtgc tgcattaccc aggcatgaac caccgggtag
661 aaatcacaga aggcatattg gcggacgaat gtgcggcgct gttgtgttac ttttttcgca
721 tgcccaggca ggtctttaac gcccagaaaa aagcacaatc ctctactgac tctggtggtt
781 cttctggtgg ttcttccggt agcgaaacac cggggacttc agaatcggcc accccggagt
841 gcctgtcata cgaaaccgag atactgacag tagaatatgg ccttctgcca atcgggaaga
901 ttgtggagaa acggatagaa tgcacagttt actctgtcga taacaatggt aacatttata
961 ctcagccagt tgcccagtgg cacgaccggg gagagcagga agtattcgaa tactgtctgg
1021 aggatggaag tctcattagg gccactaagg accacaaatt tatgacagtc gatggccaga
1081 tgctgcctat agacgaaatc tttgagcgag agttggacct catgcgagtt gacaaccttc
1141 ctaattaatg gctgaagagc ctggttaaaa aatgagctga tttaacaaaa atttaacgcg
1201 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
1261 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgaattaat
1321 tcttagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc aggattatca
1381 ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc gaggcagttc
1441 cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac atcaatacaa
1501 cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc atgagtgacg
1561 actgaatccg gtgagaatgg caaaagttta tgcatttctt tccagacttg ttcaacaggc
1621 cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt cattcgtgat
1681 tgcgcctgag ccagacgaaa tacgcgatcg ctgttaaaag gacaattaca aacaggaatc
1741 gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc tgaatcagga
1801 tattcttcta atacctggaa tgctgttttc ccggggatcg cagtggtgag taaccatgca
1861 tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc cgtcagccag
1921 tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc atgtttcaga
1981 aacaactctg gcgcatcggg cttcccatac aatcgataga ttgtcgcacc tgattgcccg
2041 acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga atttaatcgc
2101 ggcctagagc aagacgtttc ccgttgaata tggctcataa caccccttgt attactgttt
2161 atgtaagcag acagttttat tgttcatgac caaaatccct taacgtgagt tttcgttcca
2221 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
2281 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
2341 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
2401 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
2461 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
2521 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
2581 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
2641 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
2701 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
2761 gtatctttat agtcc
//
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