| 出品公司: | 爱迪基因 |
|---|---|
| 载体名称: | pCS2+8 |
| 质粒类型: | 克隆载体；哺乳动物表达载体；广宿主载体；体外表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | simian CMV IE94、lac、T3、SP6 |
| 载体大小: | 4139 bp |
| 5' 测序引物及序列: | SP6 fwd（MCS1）、T3 fwd（MCS2） |
| 3' 测序引物及序列: | EBV rev（MCS1）、SV40 polyA rev（MCS2） |
| 载体标签: | 无 |
| 载体抗性: | 氨苄青霉素 |
| 克隆菌株: | DH10B |
| 表达细胞: | 原核细胞、哺乳动物、非洲爪蟾、鸟类、斑马鱼。 |
| 备注: | pCS2+8载体能够在多种细胞中进行高水平的瞬表达。pCS2+8载体 同时也能进行体外转录和翻译。 |
| 产品目录号: | #34931 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Exported 4139 bp ds-DNA circular SYN 31-JUL-2016
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pCS2+8|
FEATURES Location/Qualifiers
source 1..4139
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 35..53
/note="SP6 promoter"
/note="promoter for bacteriophage SP6 RNA polymerase"
/note="color: #ffffff; direction: RIGHT"
misc_feature 79..165
/note="MCS1"
/note="color: #00ccff"
promoter complement(167..184)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
/note="color: #ffffff; direction: LEFT"
polyA_signal 189..323
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
/note="color: #a6acb3"
misc_feature 390..423
/note="MCS2"
/note="color: #00ccff"
promoter complement(436..454)
/note="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
/note="color: #ffffff; direction: LEFT"
misc_feature complement(477..479)
/note="ATG"
/note="color: #ff0000; direction: LEFT"
protein_bind 499..515
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/note="color: #31849b"
promoter complement(523..553)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
/note="This reverse directional feature has 3 segments:
1: 523 .. 529 / #ffffff / -10
2: 530 .. 547 / #ffffff
3: 548 .. 553 / #ffffff / -35"
protein_bind 568..589
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
/note="color: #31849b"
rep_origin complement(877..1465)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
/note="color: #ffff00"
CDS complement(1636..2496)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/note="This feature has 2 segments:
1: 1636 .. 2427 / #ccffcc
2: 2428 .. 2496 / #ccffcc / signal sequence
Cleavage site after base 2427"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2497..2601)
/gene="bla"
/note="AmpR promoter"
/note="color: #ffffff; direction: LEFT"
rep_origin complement(2627..3082)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
/note="color: #ffff00"
promoter 3156..1
/note="CMV IE94 promoter"
/note="enhancer/promoter region of simian cytomegalovirus
major immediate early transcription unit IE94"
/note="color: #ffffff; direction: RIGHT"
ORIGIN
1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
61 ctacttgttc tttttgcagg atccactagt ggcgcgccat taattaaagg ccggccagcg
121 atcgccggac atcgattgga attcaaggcc tctcgagcct ctagaactat agtgagtcgt
181 attacgtaga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
241 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
301 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
361 agggggaggt gtgggaggtt ttttaattcg cggccgcggc gccaatgcat tgggcccggt
421 acccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata
481 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
541 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
601 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
661 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
721 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
781 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
841 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
901 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
961 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
1021 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
1081 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
1141 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
1201 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
1261 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
1321 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
1381 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
1441 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
1501 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
1561 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
1621 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
1681 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
1741 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
1801 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
1861 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
1921 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
1981 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
2041 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
2101 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
2161 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
2221 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
2281 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
2341 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
2401 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
2461 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
2521 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
2581 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt
2641 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
2701 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
2761 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
2821 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
2881 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
2941 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
3001 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
3061 aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg
3121 cgatcggtgc gggcctcttc gctattacgc cagtcgacca tagccaattc aatatggcgt
3181 atatggactc atgccaattc aatatggtgg atctggacct gtgccaattc aatatggcgt
3241 atatggactc gtgccaattc aatatggtgg atctggaccc cagccaattc aatatggcgg
3301 acttggcacc atgccaattc aatatggcgg acttggcact gtgccaactg gggaggggtc
3361 tacttggcac ggtgccaagt ttgaggaggg gtcttggccc tgtgccaagt ccgccatatt
3421 gaattggcat ggtgccaata atggcggcca tattggctat atgccaggat caatatatag
3481 gcaatatcca atatggccct atgccaatat ggctattggc caggttcaat actatgtatt
3541 ggccctatgc catatagtat tccatatatg ggttttccta ttgacgtaga tagcccctcc
3601 caatgggcgg tcccatatac catatatggg gcttcctaat accgcccata gccactcccc
3661 cattgacgtc aatggtctct atatatggtc tttcctattg acgtcatatg ggcggtccta
3721 ttgacgtata tggcgcctcc cccattgacg tcaattacgg taaatggccc gcctggctca
3781 atgcccattg acgtcaatag gaccacccac cattgacgtc aatgggatgg ctcattgccc
3841 attcatatcc gttctcacgc cccctattga cgtcaatgac ggtaaatggc ccacttggca
3901 gtacatcaat atctattaat agtaacttgg caagtacatt actattggaa ggacgccagg
3961 gtacattggc agtactccca ttgacgtcaa tggcggtaaa tggcccgcga tggctgccaa
4021 gtacatcccc attgacgtca atggggaggg gcaatgacgc aaatgggcgt tccattgacg
4081 taaatgggcg gtaggcgtgc ctaatgggag gtctatataa gcaatgctcg tttagggaa
//
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