| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-SHANK2-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001411 |
| 产品用途: | 3' AAVLINK plasmid for SHANK2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | SHANK2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-SHANK2-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-SHANK2-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-SHANK2-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-SHANK2-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-SHANK2-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-SHANK2-FLAG 7168 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7168)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 6, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7168
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3881
/codon_start=1
/label=SHANK2
/translation="GSSMEIDPQAPEPPSQLRPDESLTVSSPFAAAIAGAVRDREKRLE
ARRNSPAFLSTDLGDEDVGLGPPAPRTRPSMFPEEGDFADEDSAEQLSSPMPSATPREP
ENHFVGGAEASAPGEAGRPLNSTSKAQGPESSPAVPSASSGTAGPGNYVHPLTGRLLDP
SSPLALALSARDRAMKESQQGPKGEAPKADLNKPLYIDTKMRPSLDAGFPTVTRQNTRG
PLRRQETENKYETDLGRDRKGDDKKNMLIDIMDTSQQKSAGLLMVHTVDATKLDNALQE
EDEKAEVEMKPDSSPSEVPEGVSETEGALQISAAPEPTTVPGRTIVAVGSMEEAVILPF
RIPPPPLASVDLDEDFIFTEPLPPPLEFANSFDIPDDRAASVPALSDLVKQKKSDTPQS
PSLNSSQPTNSADSKKPASLSNCLPASFLPPPESFDAVADSGIEEVDSRSSSDHHLETT
STISTVSSISTLSSEGGENVDTCTVYADGQAFMVDKPPVPPKPKMKPIIHKSNALYQDA
LVEEDVDSFVIPPPAPPPPPGSAQPGMAKVLQPRTSKLWGDVTEIKSPILSGPKANVIS
ELNSILQQMNREKLAKPGEGLDSPMGAKSASLAPRSPEIMSTISGTRSTTVTFTVRPGT
SQPITLQSRPPDYESRTSGTRRAPSPVVSPTEMNKETLPAPLSAATASPSPALSDVFSL
PSQPPSGDLFGLNPAGRSRSPSPSILQQPISNKPFTTKPVHLWTKPDVADWLESLNLGE
HKEAFMDNEIDGSHLPNLQKEDLIDLGVTRVGHRMNIERALKQLLDR"
misc_feature 1620..1625
/label=SDS
misc_feature 1717..1722
/label=SDS
misc_feature 2508..2513
/label=SDS
misc_feature 2568..2573
/label=SDS
misc_feature 2809..2814
/label=SDS
misc_feature 2973..2978
/label=SDS
misc_feature 3799..3804
/label=SDS
CDS 3888..3953
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3957..4388
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4101..4106
/label=SDS
repeat_region 4431..4571
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4431..4560
/label=AAV2 ITR
rep_origin 4646..5101
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5383..5487
/gene="bla"
/label=AmpR promoter
CDS 5488..6348
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6519..7107
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca gggcagcagc atggagatcg
1501 acccccaggc cccggagcca ccgagccagc tgcggcctga cgaaagcctg accgtcagca
1561 gcccctttgc cgccgccatc gccggagccg tccgcgaccg tgagaagcgg ctggaagcca
1621 ggaggaactc cccggccttc ctctccacag acctggggga tgaggatgtg ggcctggggc
1681 cacccgcccc caggacgcgg ccctccatgt tccccgagga gggggatttt gctgacgagg
1741 acagcgctga gcagctgtca tcccccatgc cgagtgccac gcccagggag cccgaaaacc
1801 atttcgtggg tggcgccgag gccagtgctc cgggtgaggc tgggaggccg ctgaattcca
1861 cgtccaaagc ccaggggccc gagagcagcc cagcagtgcc ctccgcgagc agcggcacag
1921 ccggccccgg gaattatgtc cacccactca cagggcggct gcttgatccc agctccccgc
1981 tggccctggc actctccgca agggaccgag ccatgaagga gtctcaacag ggacccaaag
2041 gggaggcccc caaggccgac ctcaacaaac ctctttacat tgataccaaa atgcggccca
2101 gcctggatgc cggcttccct acggtcacca ggcagaacac ccggggaccc ctgaggcggc
2161 aggagacgga gaacaagtac gagaccgacc tgggccgaga ccggaaaggc gatgacaaga
2221 agaacatgct gatcgacatc atggacacgt cccagcagaa gtcggctggc ctgctgatgg
2281 tgcacaccgt ggacgccact aagctggaca acgccctgca ggaagaggac gagaaggcag
2341 aggtggagat gaagccagac agctcgccgt ccgaggtgcc agaaggtgtt tccgaaaccg
2401 aaggtgcttt acagatctcc gctgcccccg agcccaccac cgtgcccggc agaaccatcg
2461 tcgcggtggg ctccatggaa gaggcggtga ttttgccatt ccgcatccct cctccccctc
2521 tggcatccgt ggacttggat gaggatttta tttttacaga gccattgcct cctcccctgg
2581 aatttgcaaa tagttttgat atccccgatg accgggcagc ttctgtcccg gctctctcag
2641 acttagtgaa gcagaagaaa agcgacaccc ctcagtcccc ttcgttgaac tccagccaac
2701 caaccaactc tgcagacagc aagaagccag ccagtctttc aaactgtctg cctgcctcat
2761 tcctgccacc ccctgaaagc tttgacgccg tcgccgactc tgggatcgag gaggtggaca
2821 gccggagtag cagcgaccac cacctcgaga cgaccagcac tatctccacc gtgtctagca
2881 tctccaccct gtcttccgaa ggtggagaga atgtggacac ctgcacagtc tatgcagatg
2941 ggcaagcatt tatggttgac aaacccccag tacctcctaa gccaaaaatg aagcccatca
3001 ttcacaaaag caatgcactt tatcaagacg cgctcgtgga agaagatgta gatagctttg
3061 ttatcccccc gcccgctccc ccgcccccgc cgggcagtgc ccagcctggg atggccaagg
3121 ttctccagcc aaggacctcc aagttgtggg gcgacgtcac agagatcaaa agcccgattc
3181 tctcaggccc aaaggcaaac gttattagtg aattgaactc tatcctacag caaatgaacc
3241 gagagaaatt ggcaaagccg ggggaaggac tggattcacc aatgggagcc aagtccgcca
3301 gcctcgctcc aagaagcccg gagatcatga gcaccatctc aggtacacgg agcacgacgg
3361 tcaccttcac tgttcgcccc ggcacctccc agcccatcac cctgcagagc cggccccccg
3421 actatgaaag caggacctca ggaacaagac gtgccccaag ccctgtggtc tcgccaacag
3481 agatgaacaa agagaccctg cccgcccccc tgtctgctgc caccgcctct ccttctcccg
3541 ctctctcaga tgtctttagc cttccaagcc agcccccttc tggggatcta tttggcttga
3601 acccagcggg acgcagtagg tcgccatccc cctcgatact gcaacagcca atctcaaata
3661 agccttttac aactaaacct gtccacctgt ggactaaacc agatgtggcc gattggctgg
3721 aaagtctaaa cttgggtgaa cataaagagg ccttcatgga caatgagatc gatggcagtc
3781 acttaccaaa cctgcagaag gaggacctca tcgatcttgg ggtaactcga gtcgggcaca
3841 gaatgaacat agaaagggct ttgaaacagc tgctggacag atgtacagac tacaaagacc
3901 atgacggtga ttataaagat catgacatcg actacaagga tgacgatgac aagtaactcg
3961 agataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
4021 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
4081 ccgtatggct ttcattttct cctccttgta taaatcctgg ttagttcttg ccacggcgga
4141 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
4201 ttccgtggtg tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccatctagct
4261 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
4321 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg
4381 ttttttaaag atctggagac cggtgtggca cgtgcggacc gagcggccgc aggaacccct
4441 agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc
4501 aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag
4561 ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
4621 ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt
4681 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc
4741 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
4801 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
4861 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
4921 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct
4981 atctcgggct attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa
5041 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
5101 ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
5161 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
5221 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
5281 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
5341 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
5401 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
5461 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
5521 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
5581 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
5641 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
5701 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
5761 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
5821 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
5881 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
5941 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
6001 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
6061 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
6121 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
6181 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
6241 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
6301 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
6361 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
6421 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
6481 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
6541 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
6601 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
6661 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
6721 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
6781 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
6841 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
6901 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
6961 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
7021 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
7081 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
7141 gccttttgct ggccttttgc tcacatgt
//
pAAVLINKv1-pCALM1-Cre-3'-SHANK2-FLAG载体图谱质粒图谱pdf版和pAAVLINKv1-pCALM1-Cre-3'-SHANK2-FLAG载体序列质粒序列等相关资料下载
pAAVLINKv1-pCALM1-Cre-3'-SHANK2-FLAG质粒载体应用举例
- 上一篇：pAAVLINK-EF1a-HA-5'-SHANK2
- 下一篇：没有了
