| 出品公司: | Addgene |
|---|---|
| 产品编号: | 14430 |
| 出品人: | L. Miguel Martins |
| 载体名称: | pBABE-puro-IRES-EGFP |
| 质粒类型: | 逆病毒载体；双顺反子载体；荧光报告载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | MSCV |
| 载体大小: | 6422 bp |
| 5' 测序引物及序列: | pBABE 5' primer 5'CTTTATCCAGCCCTCAC3' |
| 3' 测序引物及序列: | IRES-R primer 5'CCTCACATTGCCAAAAGACG3' |
| 载体标签: | 无 |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | Puromycin和EGFP |
| 克隆菌株: | DH5α、HB101等 |
| 宿主细胞（系）: | 常规哺乳动物细胞系 |
| 备注: |
推荐使用逆病毒包装载体：pUMVC、pCMV-VSV-G 。 |
| 产品目录号: | #14430 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 逆转录病毒 |
买家导航
pBABE-puro-IRES-EGFP载体质粒基本信息
pBABE-puro-IRES-EGFP质粒图谱载体图谱和pBABE-puro-IRES-EGFP载体序列质粒序列多克隆位点信息
pBABE-puro-IRES-EGFP质粒载体简介
pBABE-puro-IRES-EGFP质粒序列载体序列
LOCUS pBabe puro IRES-EGFP 6422 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pBabe puro IRES-EGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6422)
TITLE pBabe-IRES-EGFP plasmids
REFERENCE 2 (bases 1 to 6422)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6422
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 117..533
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 463..485
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 501..517
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
misc_feature 611..1197
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(778..795)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 1005..1024
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 1198..1917
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 1198..1914
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(1243..1264)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1504..1523)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1851..1872
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(1925..1945)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 1930..2259
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2110..2245
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2172..2191
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 2269..2868
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(2269..2288)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 2765..2785
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
LTR 3091..3560
/note="long terminal repeat from Moloney murine leukemia
virus"
primer_bind complement(3690..3707)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3861..4449)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3941..3960)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4609..5469)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5232..5251
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5470..5574)
/gene="bla"
/label=AmpR promoter
LTR 5605..6074
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 6139..6338
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
ORIGIN
1 ctgaattttt gctttcggtt tggaaccgaa gccgcgcgtc ttgtctgctg cagcgctgca
61 gcatcgttct gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa aattagggcc
121 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
181 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
241 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
301 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
361 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
421 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
481 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gccggccgga
541 tccgggctcg agcccgaatt cgggagatct cccatcgatg gggtcgacgg taccgcgggc
601 ccgggatccg cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
661 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
721 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
781 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
841 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
901 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
961 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
1021 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
1081 ggcctcggta cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
1141 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacaaccatg
1201 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
1261 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
1321 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
1381 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
1441 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
1501 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
1561 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
1621 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
1681 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
1741 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
1801 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
1861 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaagcc
1921 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
1981 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
2041 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
2101 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
2161 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
2221 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttaccat gaccgagtac
2281 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
2341 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
2401 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
2461 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
2521 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
2581 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
2641 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
2701 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
2761 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
2821 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac
2881 gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcgcgata aaataaaaga
2941 ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc
3001 tagcttaagt aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagagaagt
3061 tcagatcaag gtcaggaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg
3121 taagcagttc ctgccccggc tcagggccaa gaacagatgg aacagctgaa tatgggccaa
3181 acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc
3241 agatgcggtc cagccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca
3301 aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct
3361 gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggggcg
3421 ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag
3481 ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac
3541 ccgtcagcgg gggtctttca catgcagcat gtatcaaaat taatttggtt ttttttctta
3601 agtatttaca ttaaatggcc atagttgcat taatgaatcg gccaacgcgc ggggagaggc
3661 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
3721 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
3781 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
3841 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
3901 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
3961 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
4021 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
4081 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
4141 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
4201 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
4261 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
4321 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
4381 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
4441 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
4501 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatccttttg
4561 cggccgcaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
4621 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
4681 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
4741 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
4801 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
4861 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
4921 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
4981 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
5041 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
5101 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
5161 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
5221 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
5281 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
5341 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
5401 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
5461 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
5521 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
5581 tccccgaaaa gtgccacctg cagcctgaat atgggccaaa caggatatct gtggtaagca
5641 gttcctgccc cggctcaggg ccaagaacag atggaacagc tgaatatggg ccaaacagga
5701 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc
5761 ggtccagccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc
5821 tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc
5881 gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg ggcgccagtc
5941 ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat
6001 ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca
6061 gcgggggtct ttcatttggg ggctcgtccg ggatcgggag acccctgccc agggaccacc
6121 gacccaccac cgggaggcaa gctggccagc aacttatctg tgtctgtccg attgtctagt
6181 gtctatgact gattttatgc gcctgcgtcg gtactagtta gctaactagc tctgtatctg
6241 gcggacccgt ggtggaactg acgagttctg aacacccggc cgcaaccctg ggagacgtcc
6301 cagggacttt gggggccgtt tttgtggccc gacctgagga agggagtcga tgtggaatcc
6361 gaccccgtca ggatatgtgg ttctggtagg agacgagaac ctaaaacagt tcccgcctcc
6421 gt
//
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