| 质粒名称: | C1-F-tractin-mCherry |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 155218 |
| 存储实验室: | Tobias Meyer |
|
载体骨架基本信息 |
|
| 载体名称: | pEGFP-C1 |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 4829bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | F-tractin (aa 9-52 of rat ITPKA) fused to mCherry |
| 物种来源: | R. norvegicus (rat) |
| 插入大小: | 951 bp |
| 基因突变: | T2A, S13L, A92T, V104T, V114I, E161K, Y193K, F198Y, D202T, I203V, Y258F, A283V, K288T, N290Y relative to RpBphP2 |
| GenBank编号 | JN247409 |
| 启动子: | CMV |
| 标签/融合蛋白: | mCherry (C terminal on insert) |
| 克隆方法: | Gibson Cloning |
|
文献引用方法 |
|
| 材料和方法部分: | C1-F-tractin-mCherry was a gift from Tobias Meyer (Addgene plasmid # 155218 ; http://n2t.net/addgene:155218 ; RRID:Addgene_155218) |
| 参考文献部分: |
Membrane-proximal F-actin restricts local membrane protrusions and directs cell migration. Bisaria A, Hayer A, Garbett D, Cohen D, Meyer T. Science. 2020 Jun 12;368(6496):1205-1210. doi: 10.1126/science.aay7794. 10.1126/science.aay7794 PubMed 32527825
|
买家导航
C1-F-tractin-mCherry载体质粒基本信息
C1-F-tractin-mCherry质粒图谱载体图谱和C1-F-tractin-mCherry载体序列质粒序列多克隆位点信息
C1-F-tractin-mCherry质粒载体简介
C1-F-tractin-mCherry质粒序列载体序列
LOCUS C1-F-tractin-mCherry 4829 bp DNA circular SYN 25-AUG-2024
DEFINITION Monitor global F-actin distribution.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4829)
AUTHORS Bisaria A, Hayer A, Garbett D, Cohen D, Meyer T
TITLE Membrane-proximal F-actin restricts local membrane protrusions and
directs cell migration.
JOURNAL Science. 2020 Jun 12;368(6496):1205-1210. doi:
10.1126/science.aay7794.
PUBMED 32527825
REFERENCE 2 (bases 1 to 4829)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science";
journalName: "Science"; date: "2020-06-12- 12"; volume: "368";
issue: "6496"; pages: "1205-1210"
FEATURES Location/Qualifiers
source 1..4829
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 49..352
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 353..556
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 507..527
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 766..775
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 772..1482
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(917..935)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 1185..1204
/label=mCherry-F
/note="mCherry, forward primer"
polyA_signal 1605..1726
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1642..1661)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1696..1715
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(1733..2188)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1870..1891)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2082..2101
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2215..2319
/gene="bla"
/label=AmpR promoter
promoter 2321..2678
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
enhancer 2321..2512
/label=SV40 enhancer
/note="enhancer for the SV40 early promoter (Herr, 1993)"
primer_bind complement(2344..2364)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2529..2664
/label=SV40 ori
/note="SV40 origin of replication"
CDS 2713..3507
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
CDS 2713..3507
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2767..2786)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3377..3396
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(3695..3714)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 3739..3786
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4115..4703
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4604..4623
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
61 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
121 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
181 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
241 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
301 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
361 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
421 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
481 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
541 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat
601 ctcgagctca agcttatggg catggcgcga ccacggggcg cggggccctg cagccccggg
661 ttggagcggg ctccgcgccg gagcgtcggg gagctgcgcc tgctcttcga agcgcgctgc
721 gccgcagtcg ccgccgcagc ggccgcaggg tcggatccac cggtcgccac catggtgagc
781 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
841 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
901 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
961 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
1021 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
1081 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
1141 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
1201 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
1261 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
1321 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag
1381 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
1441 ggccgccact ccaccggcgg catggacgag ctgtacaagt aaagcggccg cgactctaga
1501 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
1561 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
1621 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
1681 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta
1741 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
1801 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
1861 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
1921 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
1981 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
2041 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
2101 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
2161 gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga
2221 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
2281 ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc
2341 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
2401 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
2461 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
2521 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
2581 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
2641 agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga
2701 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
2761 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
2821 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
2881 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
2941 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
3001 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
3061 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
3121 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
3181 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
3241 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
3301 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
3361 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
3421 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
3481 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
3541 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
3601 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
3661 tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg
3721 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt
3781 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac
3841 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg
3901 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt
3961 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
4021 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
4081 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
4141 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
4201 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
4261 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
4321 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
4381 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
4441 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
4501 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
4561 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
4621 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
4681 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
4741 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
4801 gtattaccgc catgcattag ttattaata
//
