| 出品公司: | Origene |
|---|---|
| 载体名称: | pCas-Guide-EF1a-RFP |
| 载体又名: | pCas-Guide-EF1a-RFP载体，pCas-Guide-EF1a-RFP质粒，pCas-Guide-EF1a-RFP载体质粒 |
| 英文名称: | pCas-Guide-EF1a-RFP, pCas-Guide-EF1a-RFP Vector, pCas-Guide-EF1a-RFP Plasmid |
| 质粒类型: | 哺乳动物细胞表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | EF1a |
| 载体大小: | 10.5 kb |
| 5’测序引物: | CF3: 5’-ACGATACAAGGCTGTTAGAGAG-3’ |
| 3’测序引物: | -- |
| 载体标签: | C-Myc |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | RFP |
| 克隆菌株: | DH5α,TOP10 等 |
| 宿主细胞: | 常规细胞系（293、CHO等） |
| 备注: |
表达人密码子优化的Cas9，C-端Myc-DDK标记。
BamH I和BsmB I位点用于基因组靶序列克隆。
表达靶基因gRNA。
EF1a启动子驱动RFP红色荧光蛋白表达。
克隆后，CF3引物（GE100008）可用于对靶序列进行测序。
|
| 产品目录号: | GE100140 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pCas-Guide-EF1a-RFP载体质粒基本信息
pCas-Guide-EF1a-RFP质粒图谱载体图谱和pCas-Guide-EF1a-RFP载体序列质粒序列多克隆位点信息
pCas-Guide-EF1a-RFP质粒载体简介
pCas-Guide-EF1a-RFP质粒序列载体序列
LOCUS pCas-Guide-EF1a-RFP 10479 bp DNA circular SYN 06-JUN-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10479)
AUTHORS Ming
TITLE Direct Submission
JOURNAL Exported Jun 6, 2025 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..10479
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 90..330
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
misc_RNA 384..459
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
promoter 480..1658
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 711..1649
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
CDS 1679..2389
/codon_start=1
/product="monomeric derivative of red fluorescent protein
from Entacmaea quadricolor (Merzlyak et al., 2007)"
/label=TagRFP
/note="mammalian codon-optimized"
/translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
ARYCDLPSKLGHKLN"
protein_bind complement(2941..2974)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
enhancer 3044..3347
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 3348..3551
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 3577..3595
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 3654..7757
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 7758..7778
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 7785..7805
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 7827..7856
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 7875..7898
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
primer_bind complement(7918..7934)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
polyA_signal 7945..8567
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
rep_origin complement(8688..9276)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(9440..10300)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10415..10431
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 10436..10454
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 gaattcccca gtggaaagac gcgcaggcaa aacgcaccac gtgacggagc gtgaccgcgc
61 gccgagcgcg cgccaaggtc gggcaggaag agggcctatt tcccatgatt ccttcatatt
121 tgcatatacg atacaaggct gttagagaga taattagaat taatttgact gtaaacacaa
181 agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt
241 taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt
301 tcttgggttt atatatcttg tggaaaggac gcgggatcca ctggaccagg cagcagcgtc
361 agaagacttt tttggaacgt ctcgttttag agctagaaat agcaagttaa aataaggcta
421 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttggtg tacacgtgag
481 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
541 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
601 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
661 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
721 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
781 cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg
841 agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc
901 ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt
961 tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc
1021 aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg
1081 cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag
1141 cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg
1201 gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag
1261 ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga
1321 cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt
1381 cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt
1441 agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg
1501 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat
1561 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag
1621 tggttcaaag tttttttctt ccatttcagg tgtcgtgact atagggcggc cggacgtcat
1681 ggtgtctaag ggcgaagagc tgattaagga gaacatgcac atgaagctgt acatggaggg
1741 caccgtgaac aaccaccact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg
1801 cacccagacc atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat
1861 cctggctacc agcttcatgt acggcagcag aaccttcatc aaccacaccc agggcatccc
1921 cgacttcttt aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga
1981 agacgggggc gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta
2041 caacgtcaag atcagagggg tgaacttccc atccaacggc cctgtgatgc agaagaaaac
2101 actcggctgg gaggccaaca ccgagatgct gtaccccgct gacggcggcc tggaaggcag
2161 aagcgacatg gccctgaagc tcgtgggcgg gggccacctg atctgcaact tcaagaccac
2221 atacagatcc aagaaacccg ctaagaacct caagatgccc ggcgtctact atgtggacca
2281 cagactggaa agaatcaagg aggccgacaa agagacctac gtcgagcagc acgaggtggc
2341 tgtggccaga tactgcgacc tccctagcaa actggggcac aaacttaatt ccggactcag
2401 agcgatcgcc ggcgcgccag atctcaagct taactagatc gatgatatca gatccccggg
2461 atgcagaaat tgatgatcta ttaaacaata aagatgtcca ctaaaatgga agtttttcct
2521 gtcatacttt gttaagaagg gtgagaacag agtacctaca ttttgaatgg aaggattgga
2581 gctacggggg tgggggtggg gtgggattag ataaatgcct gctctttact gaaggctctt
2641 tactattgct ttatgataat gtttcatagt tggatatcat aatttaaaca agcaaaacca
2701 aattaagggc cagctcattc ctcccactca tgatctatag atctatagat ctctcgtggg
2761 atcattgttt ttctcttgat tcccactttg tggttctaag tactgtggtt tccaaatgtg
2821 tcagtttcat agcctgaaga acgagatcag cagcctctgt tccacataca cttcattctc
2881 agtattgttt tgccaagttc taattccatc agaagctggt cgagatccgg aacccttaat
2941 ataacttcgt ataatgtatg ctatacgaag ttattaggtc cactagttat taatagtaat
3001 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
3061 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
3121 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
3181 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg
3241 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact
3301 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
3361 ggcagtacac caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
3421 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
3481 gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
3541 taagcagagc tcgtttagtg aaccgtcaga attttgtaat acgactcact atagggcggc
3601 cgggaattcg tcgactggaa ccggtaccga ggagatctgc cgccgcgatc gccatggata
3661 agaaatactc aataggactg gatattggca caaatagcgt cggatgggct gtgatcactg
3721 atgaatataa ggttccttct aaaaagttca aggttctggg aaatacagac cgccacagta
3781 tcaaaaaaaa tcttataggg gctcttctgt ttgacagtgg agagacagcc gaagctacta
3841 gactcaaacg gacagctagg agaaggtata caagacggaa gaataggatt tgttatctcc
3901 aggagatttt ttcaaatgag atggccaaag tggatgatag tttctttcat agacttgaag
3961 agtctttttt ggtggaagaa gacaagaagc atgaaagaca tcctattttt ggaaatatag
4021 tggatgaagt tgcttatcac gagaaatatc caactatcta tcatctgaga aaaaaattgg
4081 tggattctac tgataaagcc gatttgcgcc tgatctattt ggccctggcc cacatgatta
4141 agtttagagg tcattttttg attgagggcg atctgaatcc tgataatagt gatgtggaca
4201 aactgtttat ccagttggtg caaacctaca atcaactgtt tgaagaaaac cctattaacg
4261 caagtggagt ggatgctaaa gccattcttt ctgcaagatt gagtaaatca agaagactgg
4321 aaaatctcat tgctcagctc cccggtgaga agaaaaatgg cctgtttggg aatctcattg
4381 ctttgtcatt gggtttgacc cctaatttta aatcaaattt tgatttggca gaagatgcta
4441 aactccagct ttcaaaagat acttacgatg atgatctgga taatctgttg gctcaaattg
4501 gagatcaata tgctgatttg tttttggcag ctaagaatct gtcagatgct attctgcttt
4561 cagacatcct gagagtgaat actgaaataa ctaaggctcc cctgtcagct tcaatgatta
4621 aacgctacga tgaacatcat caagacttga ctcttctgaa agccctggtt agacaacaac
4681 ttccagaaaa gtataaagaa atcttttttg atcaatcaaa aaacggatat gcaggttata
4741 ttgatggcgg cgcaagccaa gaagaatttt ataaatttat caaaccaatt ctggaaaaaa
4801 tggatggtac tgaggaactg ttggtgaaac tgaatagaga agatttgctg cgcaagcaac
4861 ggacctttga caacggctct attccccatc aaattcactt gggtgagctg catgctattt
4921 tgagaagaca agaagacttt tatccatttc tgaaagacaa tagagagaag attgaaaaaa
4981 tcttgacttt taggattcct tattatgttg gtccattggc cagaggcaat agtaggtttg
5041 catggatgac tcggaagtct gaagaaacaa ttaccccatg gaattttgaa gaagttgtcg
5101 ataaaggtgc ttcagctcaa tcatttattg aacgcatgac aaactttgat aaaaatcttc
5161 caaatgaaaa agtgctgcca aaacatagtt tgctttatga gtattttacc gtttataacg
5221 aattgacaaa ggtcaaatat gttactgaag gaatgagaaa accagcattt ctttcaggtg
5281 aacagaagaa agccattgtt gatctgctct tcaaaacaaa taggaaagtg accgttaagc
5341 aactgaaaga agattatttc aaaaaaatag aatgttttga tagtgttgaa atttcaggag
5401 ttgaagatag atttaatgct tcactgggta cataccatga tttgctgaaa attattaaag
5461 ataaagattt tttggataat gaagaaaatg aagacatcct ggaggatatt gttctgacat
5521 tgaccctgtt tgaagatagg gagatgattg aggaaagact taaaacatac gctcacctct
5581 ttgatgataa ggtgatgaaa cagcttaaaa gacgcagata tactggttgg ggaaggttgt
5641 ccagaaaatt gattaatggt attagggata agcaatctgg caaaacaata ctggattttt
5701 tgaaatcaga tggttttgcc aatcgcaatt ttatgcagct catccatgat gatagtttga
5761 catttaaaga agacatccaa aaagcacaag tgtctggaca aggcgatagt ctgcatgaac
5821 atattgcaaa tctggctggt agccctgcta ttaaaaaagg tattctccag actgtgaaag
5881 ttgttgatga attggtcaaa gtgatggggc ggcataagcc agaaaatatc gttattgaaa
5941 tggcaagaga aaatcagaca actcaaaagg gccagaaaaa ttccagagag aggatgaaaa
6001 gaatcgaaga aggtatcaaa gaactgggaa gtcagattct taaagagcat cctgttgaaa
6061 atactcaatt gcaaaatgaa aagctctatc tctattatct ccaaaatgga agagatatgt
6121 atgtggacca agaactggat attaataggc tgagtgatta tgatgtcgat cacattgttc
6181 cacaaagttt ccttaaagac gattcaatag acaataaggt cctgaccagg tctgataaaa
6241 atagaggtaa atccgataac gttccaagtg aagaagtggt caaaaagatg aaaaactatt
6301 ggagacaact tctgaacgcc aagctgatca ctcaaaggaa gtttgataat ctgaccaaag
6361 ctgaaagagg aggtttgagt gaacttgata aagctggttt tatcaaacgc caattggttg
6421 aaactcgcca aatcactaag catgtggcac aaattttgga tagtcgcatg aatactaaat
6481 acgatgaaaa tgataaactt attagagagg ttaaagtgat taccctgaaa tctaaactgg
6541 tttctgactt cagaaaagat ttccaattct ataaagtgag agagattaac aattaccatc
6601 atgcccatga tgcctatctg aatgccgtcg ttggaactgc tttgattaag aaatatccaa
6661 aacttgaaag cgagtttgtc tatggtgatt ataaagttta tgatgttagg aaaatgattg
6721 ctaagtctga gcaagaaata ggcaaagcaa ccgcaaagta tttcttttac tctaatatca
6781 tgaacttctt caaaacagaa attacacttg caaatggaga gattcgcaaa cgccctctga
6841 tcgaaactaa tggggaaact ggagaaattg tctgggataa agggagagat tttgccacag
6901 tgcgcaaagt gttgtccatg ccccaagtca atatcgtcaa gaaaacagaa gtgcagacag
6961 gcggattctc taaggagtca attctgccaa aaagaaattc cgacaagctg attgctagga
7021 aaaaagactg ggacccaaaa aaatatggtg gttttgatag tccaaccgtg gcttattcag
7081 tcctggtggt tgctaaggtg gaaaaaggga aatccaagaa gctgaaatcc gttaaagagc
7141 tgctggggat cacaattatg gaaagaagtt cctttgaaaa aaatcccatt gactttctgg
7201 aagctaaagg atataaggaa gttaaaaaag acctgatcat taaactgcct aaatatagtc
7261 tttttgagct ggaaaacggt aggaaacgga tgctggctag tgccggagaa ctgcaaaaag
7321 gaaatgagct ggctctgcca agcaaatatg tgaattttct gtatctggct agtcattatg
7381 aaaagttgaa gggtagtcca gaagataacg aacaaaaaca attgtttgtg gagcagcata
7441 agcattatct ggatgagatt attgagcaaa tcagtgaatt ttctaagaga gttattctgg
7501 cagatgccaa tctggataaa gttcttagtg catataacaa acatagagac aaaccaataa
7561 gagaacaagc agaaaatatc attcatctgt ttaccttgac caatcttgga gcacccgctg
7621 cttttaaata ctttgataca acaattgata ggaaaagata tacctctaca aaagaagttc
7681 tggatgccac tcttatccat caatccatca ctggtcttta tgaaacacgc attgatttga
7741 gtcagctggg aggtgacccc aagaaaaaac gcaaggtgga agatcctaag aaaaagcgga
7801 aagtggacac gcgtacgcgg ccgctcgagc agaaactcat ctcagaagag gatctggcag
7861 caaatgatat cctggattac aaggatgacg acgataaggt ttaaacggcc ggccgcggtc
7921 atagctgttt cctgaacaga tcccgggtgg catccctgtg acccctcccc agtgcctctc
7981 ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc
8041 atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg
8101 agcaaggggc aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc
8161 tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt
8221 ctcctgcctc agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa
8281 tttttgtttt tttggtagag gcggggtttc accatattgg ccaggctggt ctccaactcc
8341 taatctcagg tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc
8401 actgctccct tccctgtcct tctgatttta aaataactat accagcagga ggacgtccag
8461 acacagcata ggctacctgg ccatgcccaa ccggtgggac atttgagttg cttgcttggc
8521 actgtcctct catgcgttgg gtccactcag tagatgcctg ttgaattggg tacgcggcca
8581 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
8641 taacgcagga aagaacatgt ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
8701 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
8761 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
8821 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
8881 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
8941 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
9001 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
9061 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
9121 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
9181 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
9241 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
9301 gggtctgacg ctcagtggaa cgacgcgtaa ctcacgttaa gggattttgg tcatgagatt
9361 atcaaaaagg atcttcacct agatcctttt gcggccgcaa atcaatctaa agtatatatg
9421 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
9481 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
9541 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
9601 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
9661 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
9721 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
9781 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
9841 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
9901 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
9961 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
10021 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
10081 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
10141 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
10201 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
10261 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
10321 attgaagcat ttatcagggt tattgtctca tgatgatata tttttatctt gtgcaatgta
10381 acatcagaga ttttgagaca cgggccagag ctgccaggaa acagctatga ccatgtaata
10441 cgactcacta taggggatat cagctggatg gcagttaac
//
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