| 质粒名称: | pRN120 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 160696 |
| 存储实验室: | Daniel Voytas |
|
载体骨架基本信息 |
|
| 载体名称: | pTRANS_221 |
| 载体出品公司: | Voytas Lab |
| 载体抗性: | 卡那霉素 |
| 完整质粒大小: | 16395 bp |
| 载体类型: | 荧光素酶；双子座病毒复制子，植物发育调节剂 |
| 筛选标记: | 卡那霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | CmYLCV::Luc+::AtHSP |
| 物种来源: | A. thaliana (mustard weed); Firefly, Cestrum yellow leaf curling virus. |
| 插入大小: | 2384 bp |
| 启动子 | CmYLCV |
| 克隆方法: | 限制性内切酶 |
| 5' 测序引物 | ctagaagtagtcaaggcggc |
| 3' 测序引物 | ccaagaagggcggaaagatcg |
|
文献引用方法 |
|
| 材料和方法部分: | pRN120 was a gift from Daniel Voytas (Addgene plasmid # 160696 ; http://n2t.net/addgene:160696 ; RRID:Addgene_160696) |
| 参考文献部分: | Plant gene editing through de novo induction of meristems. Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF. Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 10.1038/s41587-019-0337-2. 10.1038/s41587-019-0337-2 PubMed 31844292 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pRN120 17717 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+,
CmYLCV::STM::35S terminator, Nos::WUS2::PinII terminator.
ACCESSION .
VERSION .
KEYWORDS pRN120
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 17717)
AUTHORS Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
TITLE Plant gene editing through de novo induction of meristems.
JOURNAL Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi:
10.1038/s41587-019-0337-2.
PUBMED 31844292
REFERENCE 2 (bases 1 to 17717)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..17717
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(291..1085)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature 1510..1534
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal 1612..1786
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(1843..2640)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(1954..1973)
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind 2564..2583
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
promoter complement(2703..3379)
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
primer_bind complement(2746..2766)
/label=35S promoter
/note="CaMV 35S promoter, forward primer"
protein_bind 3570..3591
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 3606..3636
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3644..3660
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 3649..3671
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 3668..3684
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 3668..3684
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
CDS 4575..6227
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/label=luciferase
/note="enhanced luc+ version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
primer_bind complement(4641..4659)
/label=LucNrev
/note="Firefly luciferase, reverse primer"
promoter 6631..6810
/label=NOS promoter
/note="nopaline synthase promoter"
promoter 6663..6846
/function="NOS promoter"
/label=NOS promoter
/note="nopaline synthase promoter"
polyA_signal 11091..11265
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
misc_feature 12947..12971
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 14272..14901
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 15330..16403
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
rep_origin 16469..16663
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
primer_bind complement(16899..16921)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 17007..17147
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 17062..17081
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(17162..17179)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(17333..204)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(17413..17432)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
1741 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga
1801 tcgactctag ctagaggatc gatccgaacc ccagagtccc gctcagaaga actcgtcaag
1861 aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa
1921 gcggtcagcc cattcgccgc caagttcttc agcaatatca cgggtagcca acgctatgtc
1981 ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt
2041 ttccaccatg atattcggca agcaggcatc gccatgtgtc acgacgagat cctcgccgtc
2101 gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgttcttc
2161 gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg
2221 atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat
2281 tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg
2341 ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac
2401 agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctggag
2461 ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga
2521 cagccgaaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa
2581 tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatccccat
2641 ggtcgatcga cagatctgcg aaagctcgag agagatagat ttgtagagag agactggtga
2701 tttcagcgtg tcctctccaa atgaaatgaa cttccttata tagaggaagg gtcttgcgaa
2761 ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc
2821 tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca
2881 tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga
2941 tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct
3001 gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc
3061 ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc
3121 accatgttca catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac
3181 gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac
3241 gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct tttctactgt
3301 ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac
3361 cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt
3421 ggagtagacg agagtgtcgt gctccaccat gttggcaagc tgctctagcc aatacgcaaa
3481 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
3541 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
3601 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
3661 tttcacacag gaaacagcta tgacatgatt acgaattcga gctcaaagtt taacgcgtta
3721 gcagaaggca tgttgttgtg actccgaggg gttgcctcaa actctatctt ataaccggcg
3781 tggaggcatg gaggcagggg tattttggtc attttaatag atagtggaaa atgacgtgga
3841 atttacttaa agacgaagtc tttgcgacaa gggggggccc acgccgaatt taatattacc
3901 ggcgtggccc ccccttatcg cgagtgcttt agcacgagcg gtccagattt aaagtagaaa
3961 atttcccgcc cactagggtt aaaggtgttc acactataaa agcatatacg atgtgatggt
4021 atttgatgga gcgtatattg tatcaggtat ttccgttgga tacgaattat tcgtacgacc
4081 ctcggtaccg atcggcgcgc ctggcagaca tactgtccca caaatgaaga tggaatctgt
4141 aaaagaaaac gcgtgaaata atgcgtctga caaaggttag gtcggctgcc tttaatcaat
4201 accaaagtgg tccctaccac gatggaaaaa ctgtgcagtc ggtttggctt tttctgacga
4261 acaaataaga ttcgtggccg acaggtgggg gtccaccatg tgaaggcatc ttcagactcc
4321 aataatggag caatgacgta agggcttacg aaataagtaa gggtagtttg ggaaatgtcc
4381 actcacccgt cagtctataa atacttagcc cctccctcat tgttaaggga gcaaaatctc
4441 agagagatag tcctagagag agaaagagag caagtagcct agaagtagtc aaggcggcga
4501 agtattcagg cacgtggcca ggaagaagaa aagccaagac gacgaaaaca ggtaagagct
4561 aagcttcctg caggatggaa gacgccaaaa acataaagaa aggcccggcg ccattctatc
4621 cgctggaaga tggaaccgct ggagagcaac tgcataaggc tatgaagaga tacgccctgg
4681 ttcctggaac aattgctttt acagatgcac atatcgaggt ggacatcact tacgctgagt
4741 acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg atatgggctg aatacaaatc
4801 acagaatcgt cgtatgcagt gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt
4861 tatttatcgg agttgcagtt gcgcccgcga acgacattta taatgaacgt gaattgctca
4921 acagtatggg catttcgcag cctaccgtgg tgttcgtttc caaaaagggg ttgcaaaaaa
4981 ttttgaacgt gcaaaaaaag ctcccaatca tccaaaaaat tattatcatg gattctaaaa
5041 cggattacca gggatttcag tcgatgtaca cgttcgtcac atctcatcta cctcccggtt
5101 ttaatgaata cgattttgtg ccagagtcct tcgataggga caagacaatt gcactgatca
5161 tgaactcctc tggatctact ggtctgccta aaggtgtcgc tctgcctcat agaactgcct
5221 gcgtgagatt ctcgcatgcc agagatccta tttttggcaa tcaaatcatt ccggatactg
5281 cgattttaag tgttgttcca ttccatcacg gttttggaat gtttactaca ctcggatatt
5341 tgatatgtgg atttcgagtc gtcttaatgt atagatttga agaagagctg tttctgagga
5401 gccttcagga ttacaagatt caaagtgcgc tgctggtgcc aaccctattc tccttcttcg
5461 ccaaaagcac tctgattgac aaatacgatt tatctaattt acacgaaatt gcttctggtg
5521 gcgctcccct ctctaaggaa gtcggggaag cggttgccaa gaggttccat ctgccaggta
5581 tcaggcaagg atatgggctc actgagacta catcagctat tctgattaca cccgaggggg
5641 atgataaacc gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag gttgtggatc
5701 tggataccgg gaaaacgctg ggcgttaatc aaagaggcga actgtgtgtg agaggtccta
5761 tgattatgtc cggttatgta aacaatccgg aagcgaccaa cgccttgatt gacaaggatg
5821 gatggctaca ttctggagac atagcttact gggacgaaga cgaacacttc ttcatcgttg
5881 accgcctgaa gtctctgatt aagtacaaag gctatcaggt ggctcccgct gaattggaat
5941 ccatcttgct ccaacacccc aacatcttcg acgctggtgt cgcaggtctt cccgacgatg
6001 acgccggtga acttcccgcc gccgttgttg ttttggagca cggaaagacg atgacggaaa
6061 aagagatcgt ggattacgtc gccagtcaag taacaaccgc gaaaaagttg cgcggaggag
6121 ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca
6181 gagagatcct cataaaggcc aagaagggcg gaaagatcgc cgtgtgacgt cgacgatatg
6241 aagatgaaga tgaaatattt ggtgtgtcaa ataaaaagct tgtgtgctta agtttgtgtt
6301 tttttcttgg cttgttgtgt tatgaatttg tggctttttc taatattaaa tgaatgtaag
6361 atcacattat aatgaataaa caaatgtttc tataatccat tgtgaatgtt ttgttggatc
6421 tcttctgcag catataacta ctgtatgtgc tatggtatgg actatggaat atgattaaag
6481 ataagccaga gctctggtga cggacggcgc gactagtttt acgtacgtta attaacccgg
6541 gcgcgccgat catgagcgga gaattaaggg agtcacgtta tgacccccgc cgatgacgcg
6601 ggacaagccg ttttacgttt ggaactgaca gaaccgcaac gttgaaggag ccactcagcc
6661 gcgggtttct ggagtttaat gagctaagca catacgtcag aaaccattat tgcgcgttca
6721 aaagtcgcct aaggtcacta tcagctagca aatatttctt gtcaaaaatg ctccactgac
6781 gttccataaa ttcccctcgg tatccaatta gagtctcata ttcactctca atccaaataa
6841 tctgcaccgt acctgcaggg tccgagctag gtcacagaag cgctcaggaa ggccgctgag
6901 atagaggcat ggcggccaat gcgggcggcg gtggagcggg aggaggcagc ggcagcggca
6961 gcgtggctgc gccggcggtg tgccgcccca gcggctcgcg gtggacgccg acgccggagc
7021 agatcaggat gctgaaggag ctgtactacg gctgcggcat ccggtcgccc agctcggagc
7081 agatccagcg catcaccgcc atgctgcggc agcacggcaa gatcgagggc aagaacgtct
7141 tctactggtt ccagaaccac aaggcccgcg agcgccagaa gcgccgcctc accagcctcg
7201 acgtgaacgt gcccgccgcc ggcgcggccg acgccaccac cagccaactc ggcgtcctct
7261 cgctgtcgtc gccgccgcct tcaggcgcgg cgcctccctc gcccaccctc ggcttctacg
7321 ccgccggcaa tggcggcgga tcggctgtgc tgctggacac gagttccgac tggggcagca
7381 gcggcgctgc gatggccacc gagacatgct tcctccagga ctacatgggc gtgacggaca
7441 cgggcagctc gtcgcagtgg ccacgcttct cgtcgtcgga cacgataatg gcggcggccg
7501 cggcgcgggc ggcgacgacg cgggcgcccg agactctccc tctcttcccg acctgcggcg
7561 acgacggcgg cagcggtagc agcagctact tgccgttctg gggtgccgcg tccacaactg
7621 ccggcgccac ttcttccgtt gcgatccagc agcaacacca gctgcaggag cagtacagct
7681 tttacagcaa cagcaacagc acccagctgg ccggcaccgg caaccaagac gtatcggcaa
7741 cagcagcagc agccgccgcc ctggagctga gcctcagctc atggtgctcc ccttaccctg
7801 ctgcagggag tatgtgagag caacgcgagc tgccactgct cttcacttat gtctctggaa
7861 tggaaggagg aggaagtgag catagcgttg gtgcgttgct gtcattgtcc taggttagta
7921 gctagtgcca gttactagta agcatcaggc ataggagtat gtagtagaag catgcacgtt
7981 gccggccagc caggctttag acgggaaaag aatttggtgc agccggctgc aaaacaggat
8041 gtttacagcc cccccctcga gccctagact tgtccatctt ctggattggc caagttaatt
8101 aatgtatgaa ataaaaggat gcacacatag tgacatgcta atcactataa tgtgggcatc
8161 aaagttgtgt gttatgtgta attactaatt atctgaataa gagaaagaga tcatccatat
8221 ttcttatcct aaatgaatgt cacgtgtctt tataattctt tgatgaacca gatgcatttt
8281 attaaccaat tccatataca tataaatatt aatcatatat aattaatatc aattgggtta
8341 gcaaaacaaa tctagtctag gtgtgttttg ctaattattg ggggatagtg caaaaagaaa
8401 tctacgttct caataattca gatagaaaac ttaataaagt gagataattt acatagattg
8461 cttttatcct ttgatatatg tgaaaccatg catgatataa ggaaaataga tagagaaata
8521 attttttaca tcgttgaata tgtaaacaat ttaattcaag aagctaggaa tataaatatt
8581 gaggagttta tgattagagc tctcccgcgt cgagctgcag gtcaacggat caggatattc
8641 ttgtttaaga tgttgaactc tatggaggtt tgtatgaact gatgatctag gaccggataa
8701 gttcccttct tcatagcgaa cttattcaaa gaatgttttg tgtatcattc ttgttacatt
8761 gttattaatg aaaaaatatt attggtcatt ggactgaaca cgagtgttaa atatggacca
8821 ggccccaaat aagatccatt gatatatgaa ttaaataaca agaataaatc gagtcaccaa
8881 accacttgcc ttttttaacg agacttgttc accaacttga tacaaaagtc attatcctat
8941 gcaaatcaat aatcatacaa aaatatccaa taacactaaa aaattaaaag aaatggataa
9001 tttcacaata tgttatacga taaagaagtt acttttccaa gaaattcact gattttataa
9061 gcccacttgc attagataaa tggcaaaaaa aaacaaaaag gaaaagaaat aaagcacgaa
9121 gaattctaga aaatacgaaa tacgcttcaa tgcagtggga cccacggttc aattattgcc
9181 aattttcagc tccaccgtat atttaaaaaa taaaacgata atgctaaaaa aatataaatc
9241 gtaacgatcg ttaaatctca acggctggat cttatgacga ccgttagaaa ttgtggttgt
9301 cgacgagtca gtaataaacg gcgtcaaagt ggttgcagcc ggcacacacg agtcgtgttt
9361 atcaactcaa agcacaaata cttttcctca acctaaaaat aaggcaatta gccaaaaaca
9421 actttgcgtg taaacaacgc tcaatacacg tgtcatttta ttattagcta ttgcttcacc
9481 gccttagctt tctcgtgacc tagtcgtcct cgtcttttct tcttcttctt ctataaaaca
9541 atacccaaag agctcttctt cttcacaatt cagatttcaa tttctcaaaa tcttaaaaac
9601 tttctctcaa ttctctctac cgtgatcaag gtaaatttct gtgttcctta ttctctcaaa
9661 atcttcgatt ttgttttcgt tcgatcccaa tttcgtatat gttctttggt ttagattctg
9721 ttaatcttag atcgaacacg attttctggg tttgatcgtt agatatcatc ttaattctcg
9781 attagggttt catagatatc atccgatttg ttcaaataat ttgagttttg tcgaataatt
9841 actcttcgat ttgtgatttc tatctagatc tggtgttagt ttctagtttg tgcgatcgaa
9901 tttgtcgatt aatctgagtt tttctgatta acaggatgga gagtggttcc aacagcactt
9961 cttgtccaat ggcttttgcc ggggataata gtgatggtcc gatgtgtcct atgatgatga
10021 tgatgccgcc catcatgaca tcacatcaac atcatggtca tgatcatcaa catcaacaac
10081 aagaacatga tggttatgca tatcagtcac accaccaaca aagtagttcc ctttttcttc
10141 aatcactagc tcctccccaa ggaactaaga acaaagttgc ttcttcttct tctccttcct
10201 cttgtgctcc tgcctattct ctaatggaga tccatcataa cgaaatcgtt gcaggaggaa
10261 tcaacccttg ctcctcttcc tcttcttcag cctctgtcaa ggccaagatc atggctcatc
10321 ctcactacca ccgcctcttg gccgcttatg tcaattgtca gaaggttgga gcaccaccgg
10381 aggttgtggc gaggctagag gaggcatgct cgtctgccgc agccgctgcc gcatctatgg
10441 gaccaacagg atgtctaggt gaagatccag ggcttgatca attcatggaa gcttactgtg
10501 aaatgctcgt taagtatgag caagagctct ccaaaccttt caaggaagct atggtcttcc
10561 ttcaacgtgt cgagtgtcaa ttcaaatccc tctctctatc ctcaccttcc tctttctccg
10621 gttatggaga gacagcaatt gataggaaca ataatgggtc atccgaggaa gaagtcgata
10681 tgaacaatga atttgtagat ccacaagctg aggatagaga gcttaaagga cagctcttgc
10741 gcaagtacag tggttactta gggagcctca agcaagagtt catgaagaag aggaagaaag
10801 gaaagctccc taaagaagct cgtcaacaac tgcttgattg gtggagccgt cactacaaat
10861 ggccttaccc ttcggagcaa caaaagctcg cccttgcgga atcaacgggg ctggaccaga
10921 aacagataaa caattggttc ataaaccaga ggaaacggca ttggaagccg tcggaggaca
10981 tgcagtttgt agtaatggac gcaacacatc ctcaccatta cttcatggat aatgtcttgg
11041 gcaatccttt cccaatggat cacatctcct ccaccatgct ttgactcgag tttctccata
11101 ataatgtgtg agtagttccc agataaggga attagggttc ctatagggtt tcgctcatgt
11161 gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa
11221 aatttctaat tcctaaaacc aaaatccagt actaaaatcc agatcccccg aattaagtgt
11281 ttgatcgccg gcggtaccga gtgtacttca agtcagtggg aaatcaataa aatgattatt
11341 ttatgaatat atttcattgt gcaagtagat agaaattaca tatgttacat aacacacgaa
11401 ataaacaaaa aaagacaatc caaaaacaaa caccccaaaa aaaataatca ctttagataa
11461 actcgtatga ggagaggcac gttcagtgac tcgacgattc ccgagcaaaa aaagtctccc
11521 cgtcacacat gtagtgggtg acgcaattat ctttaaagta atccttctgt tgacttgtca
11581 ttgataacat ccagtcttcg tcaggattgc aaagaattat agaagggatc ccacctttta
11641 ttttcttctt ttttccatat ttagggttga cagtgaaatc agactggcaa cctattaatt
11701 gcttccacaa tgggacgaac ttgaagggga tgtcgtcgat gatattatag gtggcgtgtt
11761 catcgtagtt ggtgaaatcg atggtaccgt tccaatagtt gtgtcgtccg agacttctag
11821 cccaggtggt ctttccggta cgagttggtc cgcagatgta gaggctgggg tgtcggattc
11881 cattccttcc attgtccttg ttaaatcggc catccattca aggtcagatt gagcttgttg
11941 gtatgagaca ggatgtatgt aagtataagc gtctatgctt acatggtata gatgggtttc
12001 cctccaggag tgtagatctt cgtggcagcg aagatctgat tctgtgaagg gcgacacata
12061 cggttcaggt tgtggaggga ataatttgtt ggctgaatat tccagccatt gaagctttgt
12121 tgcccattca tgagggaatt cttccttgat catgtcaaga tattcctcct tagacgttgc
12181 agtctggata atagttctcc atcgtgcgtc agatttgcga ggagaaacct tatgatctcg
12241 gaaatctcct ctggttttaa tatctccgtc ctttgatatg taatcaagga cttgtttaga
12301 gtttctagct ggctggatat tagggtgatt tccttcaaaa tcgaaaaaag aaggatccct
12361 aatacaaggt tttttatcaa gctggagaag agcatgatag tgggtagtgc catcttgatg
12421 aagctcagaa gcaacaccaa ggaagaaaat aagaaaaggt gtgagtttct cccagagaaa
12481 ctggaataaa tcatctcttt gagatgagca cttgggatag gtaaggaaaa catatttaga
12541 ttggagtctg aagttcttac tagcagaagg catgttgttg tgactccgag gggttgcctc
12601 aaactctatc ttataaccgg cgtggaggca tggaggcagg ggtattttgg tcattttaat
12661 agatagtgga aaatgacgtg gaatttactt aaagacgaag tctttgcgac aagggggggc
12721 ccacgccgaa tttaatatta ccggcgtggc ccccccttat cgcgagtgct ttagcacgag
12781 cggtccagat ttaaagtaga aaatttcccg cccactaggg ttaaaggtgt tcacactata
12841 aaagcatata cgatgtgatg gtatttgatg gagcgtatat tgtatcaggt atttccgttg
12901 gatacgaatt attcgtacga ccctcatagt ttaaactatc agtgtttgac aggatatatt
12961 ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt
13021 gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt tcccctcggg
13081 atcaaagtac tttgatccaa cccctccgct gctatagtgc agtcggcttc tgacgttcag
13141 tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga caggctgccg
13201 ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt
13261 gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct
13321 atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg
13381 ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc
13441 tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc
13501 gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag gaggccggcg
13561 cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg
13621 tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc
13681 ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca
13741 ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag
13801 aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg
13861 aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg
13921 aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca
13981 ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc
14041 ggccgggtac gtgttcgagc cgcccgcgca cggctcaacc gtgcggctgc atgaaatcct
14101 ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac
14161 cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt
14221 cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt
14281 atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc
14341 cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt
14401 gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc
14461 ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga
14521 gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt
14581 ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag
14641 cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc
14701 aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt
14761 cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc
14821 gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa
14881 attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca
14941 cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg
15001 cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc
15061 tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg
15121 cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa
15181 ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt
15241 ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg cctgccggcc ctgcaatggc
15301 actggaaccc ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac
15361 aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc
15421 agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc
15481 gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc
15541 ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg
15601 atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg
15661 gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg
15721 gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat
15781 ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg
15841 ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg
15901 tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca
15961 agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg
16021 taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc
16081 gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga
16141 tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag
16201 aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga
16261 agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga
16321 aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg
16381 gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag
16441 gggaaaaagg tcgaaaaggc ctctttcctg tggatagcac gtacattggg aacccaaagc
16501 cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt
16561 cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt
16621 taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca
16681 cagcccaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg
16741 cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca
16801 atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag
16861 gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
16921 gaaacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
16981 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
17041 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
17101 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggccc tcttccgctt
17161 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
17221 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
17281 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
17341 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
17401 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
17461 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
17521 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
17581 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
17641 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
17701 ttagcagagc gaggtat
//
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