pRN120

LOCUS       pRN120              17717 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+, 
            CmYLCV::STM::35S terminator, Nos::WUS2::PinII terminator.
ACCESSION   .
VERSION     .
KEYWORDS    pRN120
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17717)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 17717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..17717
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1510..1534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1612..1786
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1843..2640)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1954..1973)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2564..2583
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2703..3379)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(2746..2766)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    3570..3591
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3606..3636
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3644..3660
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3649..3671
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3668..3684
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3668..3684
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4575..6227
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(4641..4659)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     promoter        6631..6810
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        6663..6846
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     polyA_signal    11091..11265
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    12947..12971
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             14272..14901
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             15330..16403
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      16469..16663
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(16899..16921)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    17007..17147
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     17062..17081
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(17162..17179)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(17333..204)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(17413..17432)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     1741 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga
     1801 tcgactctag ctagaggatc gatccgaacc ccagagtccc gctcagaaga actcgtcaag
     1861 aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa
     1921 gcggtcagcc cattcgccgc caagttcttc agcaatatca cgggtagcca acgctatgtc
     1981 ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt
     2041 ttccaccatg atattcggca agcaggcatc gccatgtgtc acgacgagat cctcgccgtc
     2101 gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgttcttc
     2161 gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg
     2221 atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat
     2281 tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg
     2341 ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac
     2401 agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctggag
     2461 ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga
     2521 cagccgaaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa
     2581 tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatccccat
     2641 ggtcgatcga cagatctgcg aaagctcgag agagatagat ttgtagagag agactggtga
     2701 tttcagcgtg tcctctccaa atgaaatgaa cttccttata tagaggaagg gtcttgcgaa
     2761 ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc
     2821 tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca
     2881 tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga
     2941 tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct
     3001 gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc
     3061 ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc
     3121 accatgttca catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac
     3181 gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac
     3241 gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct tttctactgt
     3301 ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac
     3361 cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt
     3421 ggagtagacg agagtgtcgt gctccaccat gttggcaagc tgctctagcc aatacgcaaa
     3481 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3541 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     3601 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     3661 tttcacacag gaaacagcta tgacatgatt acgaattcga gctcaaagtt taacgcgtta
     3721 gcagaaggca tgttgttgtg actccgaggg gttgcctcaa actctatctt ataaccggcg
     3781 tggaggcatg gaggcagggg tattttggtc attttaatag atagtggaaa atgacgtgga
     3841 atttacttaa agacgaagtc tttgcgacaa gggggggccc acgccgaatt taatattacc
     3901 ggcgtggccc ccccttatcg cgagtgcttt agcacgagcg gtccagattt aaagtagaaa
     3961 atttcccgcc cactagggtt aaaggtgttc acactataaa agcatatacg atgtgatggt
     4021 atttgatgga gcgtatattg tatcaggtat ttccgttgga tacgaattat tcgtacgacc
     4081 ctcggtaccg atcggcgcgc ctggcagaca tactgtccca caaatgaaga tggaatctgt
     4141 aaaagaaaac gcgtgaaata atgcgtctga caaaggttag gtcggctgcc tttaatcaat
     4201 accaaagtgg tccctaccac gatggaaaaa ctgtgcagtc ggtttggctt tttctgacga
     4261 acaaataaga ttcgtggccg acaggtgggg gtccaccatg tgaaggcatc ttcagactcc
     4321 aataatggag caatgacgta agggcttacg aaataagtaa gggtagtttg ggaaatgtcc
     4381 actcacccgt cagtctataa atacttagcc cctccctcat tgttaaggga gcaaaatctc
     4441 agagagatag tcctagagag agaaagagag caagtagcct agaagtagtc aaggcggcga
     4501 agtattcagg cacgtggcca ggaagaagaa aagccaagac gacgaaaaca ggtaagagct
     4561 aagcttcctg caggatggaa gacgccaaaa acataaagaa aggcccggcg ccattctatc
     4621 cgctggaaga tggaaccgct ggagagcaac tgcataaggc tatgaagaga tacgccctgg
     4681 ttcctggaac aattgctttt acagatgcac atatcgaggt ggacatcact tacgctgagt
     4741 acttcgaaat gtccgttcgg ttggcagaag ctatgaaacg atatgggctg aatacaaatc
     4801 acagaatcgt cgtatgcagt gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt
     4861 tatttatcgg agttgcagtt gcgcccgcga acgacattta taatgaacgt gaattgctca
     4921 acagtatggg catttcgcag cctaccgtgg tgttcgtttc caaaaagggg ttgcaaaaaa
     4981 ttttgaacgt gcaaaaaaag ctcccaatca tccaaaaaat tattatcatg gattctaaaa
     5041 cggattacca gggatttcag tcgatgtaca cgttcgtcac atctcatcta cctcccggtt
     5101 ttaatgaata cgattttgtg ccagagtcct tcgataggga caagacaatt gcactgatca
     5161 tgaactcctc tggatctact ggtctgccta aaggtgtcgc tctgcctcat agaactgcct
     5221 gcgtgagatt ctcgcatgcc agagatccta tttttggcaa tcaaatcatt ccggatactg
     5281 cgattttaag tgttgttcca ttccatcacg gttttggaat gtttactaca ctcggatatt
     5341 tgatatgtgg atttcgagtc gtcttaatgt atagatttga agaagagctg tttctgagga
     5401 gccttcagga ttacaagatt caaagtgcgc tgctggtgcc aaccctattc tccttcttcg
     5461 ccaaaagcac tctgattgac aaatacgatt tatctaattt acacgaaatt gcttctggtg
     5521 gcgctcccct ctctaaggaa gtcggggaag cggttgccaa gaggttccat ctgccaggta
     5581 tcaggcaagg atatgggctc actgagacta catcagctat tctgattaca cccgaggggg
     5641 atgataaacc gggcgcggtc ggtaaagttg ttccattttt tgaagcgaag gttgtggatc
     5701 tggataccgg gaaaacgctg ggcgttaatc aaagaggcga actgtgtgtg agaggtccta
     5761 tgattatgtc cggttatgta aacaatccgg aagcgaccaa cgccttgatt gacaaggatg
     5821 gatggctaca ttctggagac atagcttact gggacgaaga cgaacacttc ttcatcgttg
     5881 accgcctgaa gtctctgatt aagtacaaag gctatcaggt ggctcccgct gaattggaat
     5941 ccatcttgct ccaacacccc aacatcttcg acgctggtgt cgcaggtctt cccgacgatg
     6001 acgccggtga acttcccgcc gccgttgttg ttttggagca cggaaagacg atgacggaaa
     6061 aagagatcgt ggattacgtc gccagtcaag taacaaccgc gaaaaagttg cgcggaggag
     6121 ttgtgtttgt ggacgaagta ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca
     6181 gagagatcct cataaaggcc aagaagggcg gaaagatcgc cgtgtgacgt cgacgatatg
     6241 aagatgaaga tgaaatattt ggtgtgtcaa ataaaaagct tgtgtgctta agtttgtgtt
     6301 tttttcttgg cttgttgtgt tatgaatttg tggctttttc taatattaaa tgaatgtaag
     6361 atcacattat aatgaataaa caaatgtttc tataatccat tgtgaatgtt ttgttggatc
     6421 tcttctgcag catataacta ctgtatgtgc tatggtatgg actatggaat atgattaaag
     6481 ataagccaga gctctggtga cggacggcgc gactagtttt acgtacgtta attaacccgg
     6541 gcgcgccgat catgagcgga gaattaaggg agtcacgtta tgacccccgc cgatgacgcg
     6601 ggacaagccg ttttacgttt ggaactgaca gaaccgcaac gttgaaggag ccactcagcc
     6661 gcgggtttct ggagtttaat gagctaagca catacgtcag aaaccattat tgcgcgttca
     6721 aaagtcgcct aaggtcacta tcagctagca aatatttctt gtcaaaaatg ctccactgac
     6781 gttccataaa ttcccctcgg tatccaatta gagtctcata ttcactctca atccaaataa
     6841 tctgcaccgt acctgcaggg tccgagctag gtcacagaag cgctcaggaa ggccgctgag
     6901 atagaggcat ggcggccaat gcgggcggcg gtggagcggg aggaggcagc ggcagcggca
     6961 gcgtggctgc gccggcggtg tgccgcccca gcggctcgcg gtggacgccg acgccggagc
     7021 agatcaggat gctgaaggag ctgtactacg gctgcggcat ccggtcgccc agctcggagc
     7081 agatccagcg catcaccgcc atgctgcggc agcacggcaa gatcgagggc aagaacgtct
     7141 tctactggtt ccagaaccac aaggcccgcg agcgccagaa gcgccgcctc accagcctcg
     7201 acgtgaacgt gcccgccgcc ggcgcggccg acgccaccac cagccaactc ggcgtcctct
     7261 cgctgtcgtc gccgccgcct tcaggcgcgg cgcctccctc gcccaccctc ggcttctacg
     7321 ccgccggcaa tggcggcgga tcggctgtgc tgctggacac gagttccgac tggggcagca
     7381 gcggcgctgc gatggccacc gagacatgct tcctccagga ctacatgggc gtgacggaca
     7441 cgggcagctc gtcgcagtgg ccacgcttct cgtcgtcgga cacgataatg gcggcggccg
     7501 cggcgcgggc ggcgacgacg cgggcgcccg agactctccc tctcttcccg acctgcggcg
     7561 acgacggcgg cagcggtagc agcagctact tgccgttctg gggtgccgcg tccacaactg
     7621 ccggcgccac ttcttccgtt gcgatccagc agcaacacca gctgcaggag cagtacagct
     7681 tttacagcaa cagcaacagc acccagctgg ccggcaccgg caaccaagac gtatcggcaa
     7741 cagcagcagc agccgccgcc ctggagctga gcctcagctc atggtgctcc ccttaccctg
     7801 ctgcagggag tatgtgagag caacgcgagc tgccactgct cttcacttat gtctctggaa
     7861 tggaaggagg aggaagtgag catagcgttg gtgcgttgct gtcattgtcc taggttagta
     7921 gctagtgcca gttactagta agcatcaggc ataggagtat gtagtagaag catgcacgtt
     7981 gccggccagc caggctttag acgggaaaag aatttggtgc agccggctgc aaaacaggat
     8041 gtttacagcc cccccctcga gccctagact tgtccatctt ctggattggc caagttaatt
     8101 aatgtatgaa ataaaaggat gcacacatag tgacatgcta atcactataa tgtgggcatc
     8161 aaagttgtgt gttatgtgta attactaatt atctgaataa gagaaagaga tcatccatat
     8221 ttcttatcct aaatgaatgt cacgtgtctt tataattctt tgatgaacca gatgcatttt
     8281 attaaccaat tccatataca tataaatatt aatcatatat aattaatatc aattgggtta
     8341 gcaaaacaaa tctagtctag gtgtgttttg ctaattattg ggggatagtg caaaaagaaa
     8401 tctacgttct caataattca gatagaaaac ttaataaagt gagataattt acatagattg
     8461 cttttatcct ttgatatatg tgaaaccatg catgatataa ggaaaataga tagagaaata
     8521 attttttaca tcgttgaata tgtaaacaat ttaattcaag aagctaggaa tataaatatt
     8581 gaggagttta tgattagagc tctcccgcgt cgagctgcag gtcaacggat caggatattc
     8641 ttgtttaaga tgttgaactc tatggaggtt tgtatgaact gatgatctag gaccggataa
     8701 gttcccttct tcatagcgaa cttattcaaa gaatgttttg tgtatcattc ttgttacatt
     8761 gttattaatg aaaaaatatt attggtcatt ggactgaaca cgagtgttaa atatggacca
     8821 ggccccaaat aagatccatt gatatatgaa ttaaataaca agaataaatc gagtcaccaa
     8881 accacttgcc ttttttaacg agacttgttc accaacttga tacaaaagtc attatcctat
     8941 gcaaatcaat aatcatacaa aaatatccaa taacactaaa aaattaaaag aaatggataa
     9001 tttcacaata tgttatacga taaagaagtt acttttccaa gaaattcact gattttataa
     9061 gcccacttgc attagataaa tggcaaaaaa aaacaaaaag gaaaagaaat aaagcacgaa
     9121 gaattctaga aaatacgaaa tacgcttcaa tgcagtggga cccacggttc aattattgcc
     9181 aattttcagc tccaccgtat atttaaaaaa taaaacgata atgctaaaaa aatataaatc
     9241 gtaacgatcg ttaaatctca acggctggat cttatgacga ccgttagaaa ttgtggttgt
     9301 cgacgagtca gtaataaacg gcgtcaaagt ggttgcagcc ggcacacacg agtcgtgttt
     9361 atcaactcaa agcacaaata cttttcctca acctaaaaat aaggcaatta gccaaaaaca
     9421 actttgcgtg taaacaacgc tcaatacacg tgtcatttta ttattagcta ttgcttcacc
     9481 gccttagctt tctcgtgacc tagtcgtcct cgtcttttct tcttcttctt ctataaaaca
     9541 atacccaaag agctcttctt cttcacaatt cagatttcaa tttctcaaaa tcttaaaaac
     9601 tttctctcaa ttctctctac cgtgatcaag gtaaatttct gtgttcctta ttctctcaaa
     9661 atcttcgatt ttgttttcgt tcgatcccaa tttcgtatat gttctttggt ttagattctg
     9721 ttaatcttag atcgaacacg attttctggg tttgatcgtt agatatcatc ttaattctcg
     9781 attagggttt catagatatc atccgatttg ttcaaataat ttgagttttg tcgaataatt
     9841 actcttcgat ttgtgatttc tatctagatc tggtgttagt ttctagtttg tgcgatcgaa
     9901 tttgtcgatt aatctgagtt tttctgatta acaggatgga gagtggttcc aacagcactt
     9961 cttgtccaat ggcttttgcc ggggataata gtgatggtcc gatgtgtcct atgatgatga
    10021 tgatgccgcc catcatgaca tcacatcaac atcatggtca tgatcatcaa catcaacaac
    10081 aagaacatga tggttatgca tatcagtcac accaccaaca aagtagttcc ctttttcttc
    10141 aatcactagc tcctccccaa ggaactaaga acaaagttgc ttcttcttct tctccttcct
    10201 cttgtgctcc tgcctattct ctaatggaga tccatcataa cgaaatcgtt gcaggaggaa
    10261 tcaacccttg ctcctcttcc tcttcttcag cctctgtcaa ggccaagatc atggctcatc
    10321 ctcactacca ccgcctcttg gccgcttatg tcaattgtca gaaggttgga gcaccaccgg
    10381 aggttgtggc gaggctagag gaggcatgct cgtctgccgc agccgctgcc gcatctatgg
    10441 gaccaacagg atgtctaggt gaagatccag ggcttgatca attcatggaa gcttactgtg
    10501 aaatgctcgt taagtatgag caagagctct ccaaaccttt caaggaagct atggtcttcc
    10561 ttcaacgtgt cgagtgtcaa ttcaaatccc tctctctatc ctcaccttcc tctttctccg
    10621 gttatggaga gacagcaatt gataggaaca ataatgggtc atccgaggaa gaagtcgata
    10681 tgaacaatga atttgtagat ccacaagctg aggatagaga gcttaaagga cagctcttgc
    10741 gcaagtacag tggttactta gggagcctca agcaagagtt catgaagaag aggaagaaag
    10801 gaaagctccc taaagaagct cgtcaacaac tgcttgattg gtggagccgt cactacaaat
    10861 ggccttaccc ttcggagcaa caaaagctcg cccttgcgga atcaacgggg ctggaccaga
    10921 aacagataaa caattggttc ataaaccaga ggaaacggca ttggaagccg tcggaggaca
    10981 tgcagtttgt agtaatggac gcaacacatc ctcaccatta cttcatggat aatgtcttgg
    11041 gcaatccttt cccaatggat cacatctcct ccaccatgct ttgactcgag tttctccata
    11101 ataatgtgtg agtagttccc agataaggga attagggttc ctatagggtt tcgctcatgt
    11161 gttgagcata taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa
    11221 aatttctaat tcctaaaacc aaaatccagt actaaaatcc agatcccccg aattaagtgt
    11281 ttgatcgccg gcggtaccga gtgtacttca agtcagtggg aaatcaataa aatgattatt
    11341 ttatgaatat atttcattgt gcaagtagat agaaattaca tatgttacat aacacacgaa
    11401 ataaacaaaa aaagacaatc caaaaacaaa caccccaaaa aaaataatca ctttagataa
    11461 actcgtatga ggagaggcac gttcagtgac tcgacgattc ccgagcaaaa aaagtctccc
    11521 cgtcacacat gtagtgggtg acgcaattat ctttaaagta atccttctgt tgacttgtca
    11581 ttgataacat ccagtcttcg tcaggattgc aaagaattat agaagggatc ccacctttta
    11641 ttttcttctt ttttccatat ttagggttga cagtgaaatc agactggcaa cctattaatt
    11701 gcttccacaa tgggacgaac ttgaagggga tgtcgtcgat gatattatag gtggcgtgtt
    11761 catcgtagtt ggtgaaatcg atggtaccgt tccaatagtt gtgtcgtccg agacttctag
    11821 cccaggtggt ctttccggta cgagttggtc cgcagatgta gaggctgggg tgtcggattc
    11881 cattccttcc attgtccttg ttaaatcggc catccattca aggtcagatt gagcttgttg
    11941 gtatgagaca ggatgtatgt aagtataagc gtctatgctt acatggtata gatgggtttc
    12001 cctccaggag tgtagatctt cgtggcagcg aagatctgat tctgtgaagg gcgacacata
    12061 cggttcaggt tgtggaggga ataatttgtt ggctgaatat tccagccatt gaagctttgt
    12121 tgcccattca tgagggaatt cttccttgat catgtcaaga tattcctcct tagacgttgc
    12181 agtctggata atagttctcc atcgtgcgtc agatttgcga ggagaaacct tatgatctcg
    12241 gaaatctcct ctggttttaa tatctccgtc ctttgatatg taatcaagga cttgtttaga
    12301 gtttctagct ggctggatat tagggtgatt tccttcaaaa tcgaaaaaag aaggatccct
    12361 aatacaaggt tttttatcaa gctggagaag agcatgatag tgggtagtgc catcttgatg
    12421 aagctcagaa gcaacaccaa ggaagaaaat aagaaaaggt gtgagtttct cccagagaaa
    12481 ctggaataaa tcatctcttt gagatgagca cttgggatag gtaaggaaaa catatttaga
    12541 ttggagtctg aagttcttac tagcagaagg catgttgttg tgactccgag gggttgcctc
    12601 aaactctatc ttataaccgg cgtggaggca tggaggcagg ggtattttgg tcattttaat
    12661 agatagtgga aaatgacgtg gaatttactt aaagacgaag tctttgcgac aagggggggc
    12721 ccacgccgaa tttaatatta ccggcgtggc ccccccttat cgcgagtgct ttagcacgag
    12781 cggtccagat ttaaagtaga aaatttcccg cccactaggg ttaaaggtgt tcacactata
    12841 aaagcatata cgatgtgatg gtatttgatg gagcgtatat tgtatcaggt atttccgttg
    12901 gatacgaatt attcgtacga ccctcatagt ttaaactatc agtgtttgac aggatatatt
    12961 ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt aaaagggcgt
    13021 gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt tcccctcggg
    13081 atcaaagtac tttgatccaa cccctccgct gctatagtgc agtcggcttc tgacgttcag
    13141 tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga caggctgccg
    13201 ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt
    13261 gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct
    13321 atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg
    13381 ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc
    13441 tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc
    13501 gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag gaggccggcg
    13561 cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg
    13621 tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc
    13681 ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca
    13741 ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag
    13801 aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg
    13861 aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg
    13921 aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca
    13981 ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc
    14041 ggccgggtac gtgttcgagc cgcccgcgca cggctcaacc gtgcggctgc atgaaatcct
    14101 ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac
    14161 cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt
    14221 cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt
    14281 atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc
    14341 cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt
    14401 gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc
    14461 ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga
    14521 gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt
    14581 ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag
    14641 cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc
    14701 aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt
    14761 cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc
    14821 gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa
    14881 attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca
    14941 cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg
    15001 cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc
    15061 tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg
    15121 cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa
    15181 ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt
    15241 ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg cctgccggcc ctgcaatggc
    15301 actggaaccc ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac
    15361 aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc
    15421 agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc
    15481 gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc
    15541 ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg
    15601 atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg
    15661 gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg
    15721 gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat
    15781 ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg
    15841 ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg
    15901 tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca
    15961 agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg
    16021 taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc
    16081 gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga
    16141 tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag
    16201 aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga
    16261 agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga
    16321 aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg
    16381 gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag
    16441 gggaaaaagg tcgaaaaggc ctctttcctg tggatagcac gtacattggg aacccaaagc
    16501 cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt
    16561 cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt
    16621 taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca
    16681 cagcccaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg
    16741 cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca
    16801 atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag
    16861 gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
    16921 gaaacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
    16981 tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
    17041 gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
    17101 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggccc tcttccgctt
    17161 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
    17221 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
    17281 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
    17341 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
    17401 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
    17461 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
    17521 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
    17581 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
    17641 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
    17701 ttagcagagc gaggtat
//