pPZP112

价格:2000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: --
载体名称: pPZP112
质粒类型: 植物载体;农杆菌双元表达载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: lac
载体大小: 8910 bp
5' 测序引物及序列: M13 Reverse:CAGGAAACAGCTATGAC
3' 测序引物及序列: M13/pUC Forward:CCCAGTCACGACGTTGTAAAACG
载体标签:
载体抗性: chloramphenicol、Kanamycin
筛选标记: Neomycin
克隆菌株: HB101
宿主细胞(系): 植物细胞、农杆菌
备注: 重组质粒可用蓝白斑法筛选 
产品目录号: --
稳定性: 稳表达
组成型/诱导型: 诱导型
病毒/非病毒: 非病毒

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载体质粒图谱和多克隆位点信息

pPZP112载体图谱



pPZP112 多克隆位点

载体简介

载体序列

LOCUS       pPZP112	8909 bp 	DNA	SYN
DEFINITION  pPZP112
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..8909
                     /organism="pPZP112"
                     /mol_type="other DNA"
     CDS             202..888
                     /label="ORF frame 1"
                     /translation="MNKSAAAGLLGYARVSTDDQDLTNQRAELHAAGCTKLFSEKITG
                     TRRDRPELARMLDHLRPGDVVTVTRLDRLARSTRDLLDIAERIQEAGAGLRSLAEPWA
                     DTTTPAGRMVLTVFAGIAEFERSLIIDRTRSGREAAKARGVKFGPRPTLTPAQIAHAR
                     ELIDQEGRTVKEAAALLGVHRSTLYRALERSEEVTPTEARRRGAFREDALTEADALAA
                     AENERQEEQA*"
     CDS             1187..1816
                     /label="ORF frame 2"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSA
                     RDWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVL
                     IPVQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYE
                     LPILESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI*"
     CDS             complement(2091..2684)
                     /label="ORF frame 1"
                     /translation="MGNRHQYQVVIPHTGHAGRPCGNLYVPVWKLVADHLASSSVTLR
                     QTENGHVHDAATIAGAHVIEHRNEKIWLLVALGRLPNRRRTGCRRLPGFFADSISGRL
                     PRFTGACFCLDALPLGGLRGLQLLHQVITQRRADLYRAGWFATVTPIPRAWGFQCHCR
                     AGRQPSRLRLANRPFLHTWGIPRRRCLVVLDFPCRLL*"
     CDS             2211..3317
                     /label="ORF frame 3"
                     /translation="MALEPPSPRNRRDGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQ
                     RIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRD
                     SRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLT
                     ESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRT
                     KKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVH
                     PDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSN
                     DLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS*"
     misc_feature    complement(3813..3835)
                     /label="pGEX_3_primer"
                     /translation="MALEPPSPRNRRDGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQ
                     RIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRD
                     SRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLT
                     ESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRT
                     KKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVH
                     PDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSN
                     DLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS*"
     rep_origin      complement(4232..4851)
                     /label="pBR322_origin"
                     /translation="MALEPPSPRNRRDGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQ
                     RIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRD
                     SRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLT
                     ESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRT
                     KKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVH
                     PDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSN
                     DLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS*"
     gene            complement(5106..5765)
                     /label="CAT/CamR"
                     /gene="CAT/CamR"
                     /translation="MALEPPSPRNRRDGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQ
                     RIEAEARPGESWQAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRD
                     SRSIMDVAVFRLSKRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLT
                     ESMNRYREGKGDKPGRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRT
                     KKAKNGRLVTVSEGEALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVH
                     PDYFLIDPGIGRFLYRLARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSN
                     DLPEYDLKEEAGQAGPILVMRYRNLIEGEASAGS*"
     CDS             complement(5106..5906)
                     /label="ORF frame 1"
                     /translation="MTCFFGVQSMPRASKQQARYAVGRCLMLWSSNDVTQQGSRPKTK
                     LNIMEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFLKTVKKNKHKF
                     YPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSSLWSEYHDDF
                     RQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANMDNFFAPVF
                     TMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA*"
     CDS             complement(6643..7440)
                     /label="ORF frame 3"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLD
                     EEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADR
                     YQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            complement(6646..7425)
                     /label="NeoR/KanR (variant)"
                     /gene="NeoR/KanR (variant)"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLD
                     EEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADR
                     YQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     misc_feature    complement(8278..8421)
                     /label="lacZ_a"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLD
                     EEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADR
                     YQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     misc_feature    8392..8414
                     /label="M13_pUC_fwd_primer"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLD
                     EEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADR
                     YQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     promoter        8407..8423
                     /label="M13_forward20_primer"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLD
                     EEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADR
                     YQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     promoter        complement(8489..8507)
                     /label="M13_reverse_primer"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLD
                     EEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADR
                     YQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     misc_feature    complement(8506..8528)
                     /label="M13_pUC_rev_primer"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLD
                     EEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADR
                     YQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     promoter        complement(8542..8571)
                     /label="lac_promoter"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLD
                     EEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADR
                     YQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
ORIGIN
    1 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
   61 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
  121 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
  181 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
  241 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
  301 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
  361 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
  421 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
  481 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
  541 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
  601 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
  661 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
  721 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
  781 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
  841 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
  901 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
  961 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
 1021 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
 1081 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
 1141 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
 1201 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
 1261 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
 1321 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
 1381 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
 1441 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
 1501 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
 1561 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
 1621 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
 1681 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
 1741 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
 1801 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
 1861 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
 1921 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
 1981 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
 2041 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
 2101 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
 2161 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
 2221 aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg
 2281 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
 2341 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
 2401 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
 2461 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
 2521 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
 2581 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
 2641 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
 2701 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
 2761 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
 2821 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
 2881 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
 2941 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
 3001 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
 3061 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
 3121 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
 3181 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
 3241 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
 3301 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
 3361 aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
 3421 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
 3481 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
 3541 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
 3601 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
 3661 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
 3721 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc
 3781 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
 3841 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
 3901 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
 3961 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
 4021 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
 4081 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
 4141 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
 4201 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
 4261 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
 4321 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
 4381 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
 4441 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
 4501 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
 4561 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
 4621 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
 4681 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
 4741 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
 4801 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
 4861 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg catgatatat
 4921 ctcccaattt gtgtagggct tattatgcac gcttaaaaat aataaaagca gacttgacct
 4981 gatagtttgg ctgtgagcaa ttatgtgctt agtgcatcta atcgcttgag ttaacgccgg
 5041 cgaagcggcg tcggcttgaa cgaatttcta gctagaggat cgcaccaata actgccttaa
 5101 aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
 5161 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
 5221 ttgtcgcctt gcgtataata tttgcccatt gtgaaaacgg gggcgaagaa gttgtccata
 5281 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
 5341 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
 5401 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
 5461 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
 5521 agctcaccgt ctttcattgc catacggaac tccggatgag cattcatcag gcgggcaaga
 5581 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
 5641 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
 5701 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
 5761 tccatgatgt ttaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctc
 5821 cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata
 5881 gactgtaccc caaaaaaaca tgtcataaca agaagccatg aaaaccgcca ctgcgccgtt
 5941 accaccgctg cgttcggtca aggttctgga ccagttgcgt gacggcagtt acgctacttg
 6001 cattacagct tacgaaccga acgaggctta tgtccactgg gttcgtgccc gaattgatca
 6061 caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt
 6121 gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag
 6181 tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat
 6241 cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga
 6301 tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt
 6361 taatgtactg aattaacgcc gaattaattc gggggatcta tagggacttt aggtgatctg
 6421 gattttagta ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac
 6481 aaatacaaat acatactaag ggtttcttat atgctcaaca cgtgagcgaa accctataag
 6541 aaccctaatt cccttatctg ggaactactc acacattatt atggagaaac tcgatgtcga
 6601 tcgactctag ctagaggatc gatccgaacc ccagagtccc gctcagaaga actcgtcaag
 6661 aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa
 6721 gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc
 6781 ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt
 6841 ttccaccatg atattcggca agcaggcatc gccatgtgtc acgacgagat cctcgccgtc
 6901 gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc
 6961 gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg
 7021 atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat
 7081 tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg
 7141 ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac
 7201 agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctggag
 7261 ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga
 7321 cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa
 7381 tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatccccat
 7441 ggtcgatcga cagatctgcg aatcgagaga gatagatttg tagagagaga ctggtgattt
 7501 cagcgtgtcc tctccaaatg aaatgaactt ccttatatag aggaagggtc ttgcgaagga
 7561 tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc cacttgcttt
 7621 gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct
 7681 ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc gcaatgatgg
 7741 catttgtagg tgccaccttc cttttctact gtccttttga tgaagtgaca gatagctggg
 7801 caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc aatagccctt
 7861 tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc gtgctccacc
 7921 atgttatcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg
 7981 atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaacg
 8041 atagcctttc ctttatcgca atgatggcat ttgtaggtgc caccttcctt ttctactgtc
 8101 cttttgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc
 8161 ctttgttgaa aagtctcaat agccctttgg tcttctgaga ctgtatcttt gatattcttg
 8221 gagtagacga gagtgtcgtg ctccaccatg ttggcaagct gctctagcat tcgccattca
 8281 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
 8341 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
 8401 gacgttgtaa aacgacggcc agtgccaagc ttgcatgcct gcaggtcgac tctagaggat
 8461 ccccgggtac cgagctcgaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
 8521 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
 8581 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
 8641 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
 8701 cgtattggct agagcagctt gagcttggat cagattgtcg tttcccgcct tcagtttaaa
 8761 ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag
 8821 aataacggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat
 8881 gccaaccaca gggttcccct cgggatcaa
//

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