pEarleyGate202

价格:2000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: CAMBIA
载体名称: pEarleyGate202; pEarleyGate 202
质粒类型: Gateway载体;植物载体;农杆菌双元表达载体
高拷贝/低拷贝: 高拷贝
克隆方法: Gateway
启动子: 35S
载体大小: 11776 bp
5' 测序引物及序列: 35S promoter:CTATCCTTCGCAAGACCCTTC
3' 测序引物及序列: --
载体标签: FLAG (N ter)
载体抗性: Kanamycin.html' target='_blank'>卡那霉素氯霉素(仅空载体)
筛选标记: 草丁膦(磷丝菌素)phosphinothricin、
克隆菌株: DB3.1菌株
宿主细胞(系): 植物细胞、农杆菌
备注: --
产品目录号: --
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pEarleyGate202载体图谱



载体简介

载体序列

LOCUS       pEarleyGate 202        11776 bp ds-DNA     circular SYN 26-JUL-2016
DEFINITION  Gateway(R)-compatible plant transformation vector with an N-terminal
            FLAG tag.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11776)
  AUTHORS   Earley KW, Haag JR, Pontes O, Opper K, Juehne T, Song K, Pikaard CS.
  TITLE     Gateway-compatible vectors for plant functional genomics and 
            proteomics.
  JOURNAL   Plant J. 2006;45:616-29.
  PUBMED    16441352
REFERENCE   2  (bases 1 to 11776)
  AUTHORS   Pikaard Lab
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, July 26, 2016 from SnapGene Viewer 3.1.4 to Vector
            NTI(R) format
            http://www.snapgene.com
COMMENT     The sequence was corrected by inserting a G at position 7883.
COMMENT     VNTDATE|660009600|
COMMENT     VNTDBDATE|776390400|
COMMENT     VNTNAME|pEarleyGate 202|
COMMENT     VNTAUTHORNAME|Pikaard Lab|
FEATURES             Location/Qualifiers
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      111..363
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(373..924)
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase草丁膦(磷丝菌素) 
                     乙酰转移酶"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
     promoter        complement(930..1310)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     promoter        2306..2651
                     /label=35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    2681..2735
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             2737..2739
                     /codon_start=1
                     /product="start codon"
                     /label=ATG
     CDS             2740..2763
                     /codon_start=1
                     /product="FLAG epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
     protein_bind    2767..2886
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2916..2946
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             3000..3659
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
     CDS             4001..4306
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
     protein_bind    complement(4347..4471)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      4547..5254
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     misc_feature    5498..5522
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6822..7451
                     /codon_start=1
                     /product="stability protein from Pseudomonas plasmid pVS1"
                     /label=pVS1 StaA
     CDS             7880..8953
                     /codon_start=1
                     /product="replication protein from Pseudomonas plasmid 
                     pVS1"
                     /label=pVS1 RepA
                     /translation="MDGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9019..9213
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    9557..9697
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9883..10471)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10558..11352)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcggatct gataatttat
      121 ttgaaaattc ataagaaaag caaacgttac atgaattgat gaaacaatac aaagacagat
      181 aaagccacgc acatttagga tattggccga gattactgaa tattgagtaa gatcacggaa
      241 tttctgacag gagcatgtct tcaattcagc ccaaatggca gttgaaatac tcaaaccgcc
      301 ccatatgcag gagcggatca ttcattgttt gtttggttgc ctttgccaac atgggagtcc
      361 aagattctgc agtcaaatct cggtgacggg caggaccgga cggggcggta ccggcaggct
      421 gaagtccagc tgccagaaac ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg
      481 cagcatgccg cggggggcat atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg
      541 cagcccgatg acagcgacca cgctcttgaa gccctgtgcc tccagggact tcagcaggtg
      601 ggtgtagagc gtggagccca gtcccgtccg ctggtggcgg ggggagacgt acacggtcga
      661 ctcggccgtc cagtcgtagg cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc
      721 gacctcgccg tccacctcgg cgacgagcca gggatagcgc tcccgcagac ggacgaggtc
      781 gtccgtccac tcctgcggtt cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat
      841 gtagtggttg acgatggtgc agaccgccgg catgtccgcc tcggtggcac ggcggatgtc
      901 ggccgggcgt cgttctgggc tcatcgattc gatttggtgt atcgagattg gttatgaaat
      961 tcagatgcta gtgtaatgta ttggtaattt gggaagatat aataggaagc aaggctattt
     1021 atccatttct gaaaaggcga aatggcgtca ccgcgagcgt cacgcgcatt ccgttcttgc
     1081 tgtaaagcgt tgtttggtac acttttgact agcgaggctt ggcgtgtcag cgtatctatt
     1141 caaaagtcgt taatggctgc ggatcaagaa aaagttggaa tagaaacaga atacccgcga
     1201 aattcaggcc cggttgccat gtcctacacg ccgaaataaa cgaccaaatt agtagaaaaa
     1261 taaaaactga ctcggatact tacgtcacgt cttgcgcact gatttgaaaa atctcagaat
     1321 tccaatccca caaaaatctg agcttaacag cacagttgct cctctcagag cagaatcggg
     1381 tattcaacac cctcatatca actactacgt tgtgtataac ggtccacatg ccggtatata
     1441 cgatgactgg ggttgtacaa aggcggcaac aaacggcgtt cccggagttg cacacaagaa
     1501 atttgccact attacagagg caagagcagc agctgacgcg tacacaacaa gtcagcaaac
     1561 agacaggttg aacttcatcc ccaaaggaga agctcaactc aagcccaaga gctttgctaa
     1621 ggccctaaca agcccaccaa agcaaaaagc ccactggctc acgctaggaa ccaaaaggcc
     1681 cagcagtgat ccagccccaa aagagatctc ctttgccccg gagattacaa tggacgattt
     1741 cctctatctt tacgatctag gaaggaagtt cgaaggtgaa ggtgacgaca ctatgttcac
     1801 cactgataat gagaaggtta gcctcttcaa tttcagaaag aatgctgacc cacagatggt
     1861 tagagaggcc tacgcagcag gtctcatcaa gacgatctac ccgagtaaca atctccagga
     1921 gatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaattgcat
     1981 caagaacaca gagaaagaca tatttctcaa gatcagaagt actattccag tatggacgat
     2041 tcaaggcttg cttcataaac caaggcaagt aatagagatt ggagtctcta aaaaggtagt
     2101 tcctactgaa tctaaggcca tgcatggagt ctaagattca aatcgaggat ctaacagaac
     2161 tcgccgtgaa gactggcgaa cagttcatac agagtctttt acgactcaat gacaagaaga
     2221 aaatcttcgt caacatggtg gagcacgaca ctctggtcta ctccaaaaat gtcaaagata
     2281 cagtctcaga agaccaaagg gctattgaga cttttcaaca aaggataatt tcgggaaacc
     2341 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
     2401 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatctctctg
     2461 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
     2521 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
     2581 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
     2641 tggagaggac acgctcgagt ataagagctc tatttttaca acaattacca acaacaacaa
     2701 acaacaaaca acattacaat tacatttaca attaccatgg actacaaaga cgatgacgac
     2761 aaaatcacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat
     2821 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatc
     2881 cagtcatatt ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt
     2941 ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa
     3001 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
     3061 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
     3121 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     3181 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg
     3241 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     3301 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     3361 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     3421 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     3481 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
     3541 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
     3601 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa
     3661 cgcgtggatc cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt
     3721 gcggtataag aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtatgctat
     3781 gaagcagcgt attacagtga cagttgacag cgacagctat cagttgctca aggcatatat
     3841 gatgtcaata tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt
     3901 gccgaacgct ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa
     3961 atgaacggct cttttgctga cgagaacagg ggctggtgaa atgcagttta aggtttacac
     4021 ctataaaaga gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac
     4081 gcccgggcga cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc
     4141 ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga
     4201 tatggccagt gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga
     4261 aaatgacatc aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccct
     4321 tatacacagc cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagcatt
     4381 atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg
     4441 tttctcgttc agctttcttg tacaaagtgg tgcctaggtg agtctagaga gttaatctag
     4501 gtgagtctag agagttaatt aagacccggg actagtccct agagtcctgc tttaatgaga
     4561 tatgcgagac gcctatgatc gcatgatatt tgctttcaat tctgttgtgc acgttgtaaa
     4621 aaacctgagc atgtgtagct cagatcctta ccgccggttt cggttcattc taatgaatat
     4681 atcacccgtt actatcgtat ttttatgaat aatattctcc gttcaattta ctgattgtac
     4741 cctactactt atatgtacaa tattaaaatg aaaacaatat attgtgctga ataggtttat
     4801 agcgacatct atgatagagc gccacaataa caaacaattg cgttttatta ttacaaatcc
     4861 aattttaaaa aaagcggcag aaccggtcaa acctaaaaga ctgattacat aaatcttatt
     4921 caaatttcaa aagtgcccca ggggctagta tctacgacac accgagcggc gaactaataa
     4981 cgctcactga agggaactcc ggttccccgc cggcgcgcat gggtgagatt ccttgaagtt
     5041 gagtattggc cgtccgctct accgaaagtt acgggcacca ttcaacccgg tccagcacgg
     5101 cggccgggta accgacttgc tgccccgaga attatgcagc atttttttgg tgtatgtggg
     5161 ccccaaatga agtgcaggtc aaaccttgac agtgacgaca aatcgttggg cgggtccagg
     5221 gcgaattttg cgacaacatg tcgaggctca gcaggacctg caggcatgca agcttggcac
     5281 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     5341 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     5401 cttcccaaca gttgcgcagc ctgaatggcg aatgctagag cagcttgagc ttggatcaga
     5461 ttgtcgtttc ccgccttcag tttaaactat cagtgtttga caggatatat tggcgggtaa
     5521 acctaagaga aaagagcgtt tattagaata acggatattt aaaagggcgt gaaaaggttt
     5581 atccgttcgt ccatttgtat gtgcatgcca accacagggt tcccctcggg atcaaagtac
     5641 tttgatccaa cccctccgct gctatagtgc agtcggcttc tgacgttcag tgcagccgtc
     5701 ttctgaaaac gacatgtcgc acaagtccta agttacgcga caggctgccg ccctgccctt
     5761 ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa
     5821 ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt
     5881 cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac
     5941 caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat
     6001 gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg
     6061 cagcacccgc gacctactgg acattgccga gcgcatccag gaggccggcg cgggcctgcg
     6121 tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt
     6181 gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg
     6241 cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca
     6301 gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc
     6361 actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac
     6421 gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc
     6481 cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc
     6541 caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac
     6601 gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg
     6661 tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac cgagcgccgc
     6721 cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat
     6781 atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac
     6841 ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc
     6901 aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt
     6961 gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg
     7021 accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg
     7081 cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc
     7141 caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg
     7201 aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc
     7261 gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc
     7321 gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat
     7381 cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa
     7441 aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc
     7501 cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc
     7561 agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta
     7621 cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc
     7681 agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca
     7741 tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg
     7801 gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc actggaaccc
     7861 ccaagcccga ggaatcggcg tggacggtcg caaaccatcc ggcccggtac aaatcggcgc
     7921 ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc agcggcaacg
     7981 catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa
     8041 agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga
     8101 cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag
     8161 catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat
     8221 ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggccag
     8281 tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg
     8341 ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt
     8401 actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg
     8461 cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg
     8521 cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga
     8581 aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac
     8641 agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg
     8701 catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg
     8761 gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt
     8821 caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc
     8881 ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc
     8941 cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg
     9001 tcgaaaaggt ctctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg
     9061 gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta
     9121 agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat
     9181 taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaga
     9241 gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg
     9301 gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg
     9361 gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccctgcc
     9421 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     9481 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     9541 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg
     9601 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     9661 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac
     9721 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     9781 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     9841 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     9901 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     9961 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    10021 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    10081 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    10141 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    10201 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    10261 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    10321 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    10381 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    10441 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    10501 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgcatt ctaggtacta
    10561 aaacaattca tccagtaaaa tataatattt tattttctcc caatcaggct tgatccccag
    10621 taagtcaaaa aatagctcga catactgttc ttccccgata tcctccctga tcgaccggac
    10681 gcagaaggca atgtcatacc acttgtccgc cctgccgctt ctcccaagat caataaagcc
    10741 acttactttg ccatctttca caaagatgtt gctgtctccc aggtcgccgt gggaaaagac
    10801 aagttcctct tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa
    10861 tggagtgtct tcttcccagt tttcgcaatc cacatcggcc agatcgttat tcagtaagta
    10921 atccaattcg gctaagcggc tgtctaagct attcgtatag ggacaatccg atatgtcgat
    10981 ggagtgaaag agcctgatgc actccgcata cagctcgata atcttttcag ggctttgttc
    11041 atcttcatac tcttccgagc aaaggacgcc atcggcctca ctcatgagca gattgctcca
    11101 gccatcatgc cgttcaaagt gcaggacctt tggaacaggc agctttcctt ccagccatag
    11161 catcatgtcc ttttcccgtt ccacatcata ggtggtccct ttataccggc tgtccgtcat
    11221 ttttaaatat aggttttcat tttctcccac cagcttatat accttagcag gagacattcc
    11281 ttccgtatct tttacgcagc ggtatttttc gatcagtttt ttcaattccg gtgatattct
    11341 cattttagcc atttattatt tccttcctct tttctacagt atttaaagat accccaagaa
    11401 gctaattata acaagacgaa ctccaattca ctgttccttg cattctaaaa ccttaaatac
    11461 cagaaaacag ctttttcaaa gttgttttca aagttggcgt ataacatagt atcgacggag
    11521 ccgattttga aaccgcggtg atcacaggca gcaacgctct gtcatcgtta caatcaacat
    11581 gctaccctcc gcgagatcat ccgtgtttca aacccggcag cttagttgcc gttcttccga
    11641 atagcatcgg taacatgagc aaagtctgcc gccttacaac ggctctcccg ctgacgccgt
    11701 cccggactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga
    11761 gctgttggct ggctgg
//

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