| 出品公司: | Molecularcloud |
|---|---|
| 载体名称: | pcrF11 |
| 质粒类型: | 枯草芽孢杆菌表达表达载体，Crispr载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | -- |
| 载体大小: | 4.4 kb |
| 5’测序引物: | -- |
| 3’测序引物: | -- |
| 载体标签: | -- |
| 载体抗性: | 卡那霉素 |
| 克隆菌株: | DH5 alpha |
| 宿主细胞（系）: | 枯草芽孢杆菌 |
| 产品目录号: | MC_0068419 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
| 备注: | The plasmid is used for the expression of crRNA in B. subtilis，and can replicate in E. coli. |
| 参考文献: | Wu, Y., Liu, Y., Lv, X., Li, J., Du, G., & Liu, L. (2020). CAMERS‐B: CRISPR/Cpf1 assisted multiple‐genes editing and regulation system for Bacillus subtilis. Biotechnology and Bioengineering. |
买家导航
pcrF11载体质粒基本信息
pcrF11质粒图谱载体图谱和pcrF11载体序列质粒序列多克隆位点信息
pcrF11质粒载体简介
pcrF11质粒序列载体序列
LOCUS pcrF11 4430 bp DNA circular SYN 12-NOV-2024
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4430)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Nov 12, 2024 from SnapGene 6.0.2 to Vector NTI(R) format
https://www.biofeng.com
COMMENT VNTDATE|1073779200|
COMMENT VNTDBDATE|1073779200|
COMMENT VNTNAME|pcrF11|
FEATURES Location/Qualifiers
primer_bind complement(13..29)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(35..57)
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(61..91)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(106..127)
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin 415..1003
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3168..3983)
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
ORIGIN
1 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
61 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
121 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
181 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
241 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
301 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
361 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
421 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
481 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
541 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
601 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
661 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
721 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
781 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
841 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
901 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
961 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
1021 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
1081 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
1141 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcatgcggt attttctcct
1201 tacgacaacg tcttattaac gttgatataa tttaaatttt atttgacaaa aatgggctcg
1261 tgttgtacaa taaatgtaga ggtagagacg cgaggtctaa gaactttaaa taatttctac
1321 tgttgtagat agagaccgtg aagttaataa ggtctcaaat ttctactgtt gtagatcgtc
1381 tctgaactga ttcaagcaag cttaaaccca gctcaatgag ctgggttttt tgtttgtttt
1441 ttcaaactta gttagcttgg ccgaattcgt cgactctaga ctgcagatcg tcgaacggca
1501 gatcagaatt ttgtaataaa aaaagagcct gctcattaca ctgcgggctc tttttcatgg
1561 tcagaagacg ggtaaccaag ataacaaaga atacaagaaa atatttacaa aaaatcaatt
1621 taacaattcc ttaaaacatg caggaattga cgatttaaac aatattagct ttgaacaatt
1681 cttatctctt ttcaatagct ataaattatt taataagtaa gttaagggat gcataaactg
1741 catcccttaa cttgtttttc gtgtgcccat tttttgtgaa tcgattatgt cttttgcgca
1801 gtcggcttaa accagttttc gctggtgcga aaaaagagtg tcttgtgaca ctcttaaatt
1861 caaaatctat cggtcagatt tataccgatt tgattttata tattcttgaa taacatacgc
1921 cgagttatca cataaaagcg ggaaccaatc atcaaattta aacttcattg cataatccat
1981 taaactctta aattctacga ttccttgttc atcaataaac tcaatcattt ctttaattaa
2041 tttatatcta tctgttgttg ttttctttaa taattcatca acatctacac cgccataaac
2101 tatcatatct tctttttgat atttaaattt attaggatcg tccatgtgaa gcatatatct
2161 cacaagacct ttcacacttc ctgcaatctg cggaatagtc gcattcaatt cttctgtaat
2221 tatttttatc tgttcataag atttattacc ctcatacatc actagaatat gataatgctc
2281 ttttttcatc ctatcttctg tatcagtatc cctatcatgt aatggagaca ctacaaattg
2341 aatgtgtaac tcttttaaat actctaacca ctcggctttt gctgattctg gatataaaac
2401 aaatgtccaa ttacgtcctc ttgaattttt cttgttttca gtttctttta ttacattttc
2461 gctcatgata taataacggt gctaatacat ttaacaaaat ttagtcatag ataggcagca
2521 tgccagtgct gtctatcttt ttttgtttaa aatgcaccgt attcctcctt tgcatatttt
2581 tttattagaa taccggttgc atctgatttg ctaatattat atttttcttt gattctattt
2641 aatatctcat tttcttctgt tgtaagtctt aaagtaacag caactttttt ctcttctttt
2701 ctatctacaa ccatcactgt acctcccaac atctgttttt ttcactttaa cataaaaaac
2761 aaccttttaa cattaaaaac ccaatattta tttatttgtt tggacaatgg acaatggaca
2821 cctagggggg aggtcgtagt acccccctat gttttctccc ctaaataacc ccaaaaatct
2881 aagaaaaaaa gacctcaaaa aggtctttaa ttaacatctc aaatttcgca tttattccaa
2941 tttccttttt gcgtgtgatg cgctgcgtcc attaaaaatc ctagagcttt gcaaccgaaa
3001 gttaatagct gtcgctacta ctttcgctta cgctctaagt atattttaag gactgtcaca
3061 cgcaaaaagt tttctcggca taaaagtacc tctacatctc taaatcgtct gtacgctgtt
3121 tctcacgctt tctatcgacc ttctggacct tagacgtcag gtggcactta gaaaaactca
3181 tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga
3241 aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga
3301 tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc
3361 tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag
3421 aatggcaaaa gtttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg
3481 tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga
3541 cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc
3601 aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc
3661 tggaatgctg ttttcccagg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg
3721 ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc
3781 tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca
3841 tcgggcttcc catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc
3901 catttatacc catataaatc agcatccatg ttggaattta atcgcggcct agagcaagac
3961 gtttcccgtt gaatatggct cattggatcc ttcctccttt aattgggtaa gttaattata
4021 tcacatgcta aattcagtgt caaggaggcg ctcgccagtc gattggctga gctcataagt
4081 tcctattccg aagttccgcg aacctttaag acctttcttt tttttacgag aaaaaagaaa
4141 caaaaaaacc tgccctctgc cacctcagca aaggggggtt ttgctctcgt gctcgtttaa
4201 aaatcagcaa gggacaggta gtattttttg agaagatcac tcaaaaaatc tccaccttta
4261 aacccttgcc aatttttatt ttgtccgttt tgtctagctt accgaaagcc agactcagca
4321 agaataaaat ttttattgtc tttcggtttt ctagtgtaac ggacaaaacc actcaaaata
4381 aaaaagatac aagagtggac tctcgtatct tttattcagc aatcgcgccc
//
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