| 质粒名称: | mCh-Drp1 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 49152 |
| 存储实验室: | Gia Voeltz |
|
载体骨架基本信息 |
|
| 载体名称: | mCh-alpha tubulin (Addgene plasmid ) |
| 载体出品公司: | Voeltz lab |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Drp1,DNM1L，dynamin 1-like,Drp1, isoform 3 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | NM_005690 |
| 启动子 | CMV |
| 标签/融合蛋白: | mCherry（主干上的N终端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | XhoI(未破坏) |
| 3' 克隆位点: | BamHI (未破坏) |
| 5' 测序引物 | mCherry-F (ccccgtaatgcagaagaaga) |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | mCh-Drp1 was a gift from Gia Voeltz (Addgene plasmid # 49152 ; http://n2t.net/addgene:49152 ; RRID:Addgene_49152) |
| 参考文献部分: | ER tubules mark sites of mitochondrial division. Friedman JR, Lackner LL, West M, DiBenedetto JR, Nunnari J, Voeltz GK. Science. 2011 Oct 21;334(6054):358-62. doi: 10.1126/science.1207385. Epub 2011 Sep 1. 10.1126/science.1207385 PubMed 21885730 |
买家导航
mChDrp1载体基本信息
mChDrp1载体图谱质粒图谱和多克隆位点信息
mChDrp1载体简介
mChDrp1载体序列
LOCUS mCh-Drp1 6783 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION mammalian expression of Drp1 fused to mCherry.
ACCESSION .
VERSION .
KEYWORDS mCh-Drp1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6783)
AUTHORS Friedman JR, Lackner LL, West M, DiBenedetto JR, Nunnari J, Voeltz
GK
TITLE ER tubules mark sites of mitochondrial division.
JOURNAL Science. 2011 Oct 21;334(6054):358-62. doi: 10.1126/science.1207385.
Epub 2011 Sep 1.
PUBMED 21885730
REFERENCE 2 (bases 1 to 6783)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6783
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 68..371
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 372..575
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 526..546
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 614..623
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 620..1327
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(765..783)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 1033..1052
/label=mCherry-F
/note="mCherry, forward primer"
polyA_signal 3578..3699
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3615..3634)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 3669..3688
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(3706..4161)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3843..3864)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 4055..4074
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 4188..4292
/gene="bla"
/label=AmpR promoter
promoter 4294..4651
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(4317..4337)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 4502..4637
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4564..4583
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 4686..5480
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4740..4759)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 5350..5369
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(5668..5687)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 5712..5759
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 6088..6676
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6577..6596
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
541 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
601 agcgctaccg gtcgccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
661 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
721 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
781 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
841 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
901 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
961 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
1021 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
1081 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
1141 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
1201 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
1261 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
1321 gtacaagtcc ggactcagat ctcgagtgat ggaggcgcta attcctgtca taaacaagct
1381 ccaggacgtc ttcaacacgg tgggcgccga catcatccag ctgcctcaaa tcgtcgtagt
1441 gggaacgcag agcagcggaa agagctccgt gctagaaagc ctggtgggga gggacctgct
1501 tcccagaggt actggaattg tcacccggag acctctcatt ctgcaactgg tccatgtttc
1561 acaagaagat aaacggaaaa caacaggaga agaaaatgga gtggaagcag aagaatgggg
1621 taaatttctt cacaccaaaa ataagcttta cacggatttt gatgaaattc gacaagaaat
1681 tgaaaatgaa acagaaagaa tttcaggaaa taataaggga gtaagccctg aaccaattca
1741 tcttaagatt ttttcaccca acgttgtcaa tttgacactt gtggatttgc caggaatgac
1801 caaggtgcct gtaggtgatc aacctaagga tattgagctt caaatcagag agctcattct
1861 tcggttcatc agtaatccta attccattat cctcgctgtc actgctgcta atacagatat
1921 ggcaacatca gaggcactta aaatttcaag agaggtagat ccagatggtc gcagaaccct
1981 agctgtaatc actaaacttg atctcatgga tgcgggtact gatgccatgg atgtattgat
2041 gggaagggtt attccagtca aacttggaat aattggagta gttaacagga gccagctaga
2101 tattaacaac aagaagagtg taactgattc aatccgtgat gagtatgctt ttcttcaaaa
2161 gaaatatcca tctctggcca atagaaatgg aacaaagtat cttgctagga ctctaaacag
2221 gttactgatg catcacatca gagattgttt accagagttg aaaacgagaa taaatgttct
2281 agctgctcag tatcagtctc ttctaaatag ctacggtgaa cccgtggatg ataaaagtgc
2341 tactttactc caacttatta ccaaatttgc cacagaatat tgtaacacta ttgaaggaac
2401 tgcaaaatat attgaaactt cggagctatg cggtggtgct agaatttgtt atattttcca
2461 tgagactttt gggcgaacct tagaatctgt tgatccactt ggtggcctta acactattga
2521 cattttgact gccattagaa atgctactgg tcctcgtcct gctttatttg tgcctgaggt
2581 ttcatttgag ttactggtga agcggcaaat caaacgtcta gaagagccca gcctccgctg
2641 tgtggaactg gttcatgagg aaatgcaaag gatcattcag cactgtagca attacagtac
2701 acaggaattg ttacgatttc ctaaacttca tgatgccata gttgaagtgg tgacttgtct
2761 tcttcgtaaa aggttgcctg ttacaaatga aatggtccat aacttagtgg caattgaact
2821 ggcttatatc aacacaaaac atccagactt tgctgatgct tgtgggctaa tgaacaataa
2881 tatagaggaa caaaggagaa acaggctagc cagagaatta ccttcagctg tatcacgaga
2941 caaggttgca tctggaggtg gtggggttgg agatggtgtt caagaaccaa ccacaggcaa
3001 ctggagagga atgctgaaaa cttcaaaagc tgaagagtta ttagcagaag aaaaatcaaa
3061 acccattcca attatgccag ccagtccaca aaaaggtcat gccgtgaacc tgctagatgt
3121 gccagttcct gttgcacgaa aactatctgc tcgggaacag cgagattgtg aggttattga
3181 acgactcatt aaatcatatt ttctcattgt cagaaagaat attcaagaca gtgtgccaaa
3241 ggcagtaatg cattttttgg ttaatcatgt gaaagacact cttcagagtg agctagtagg
3301 ccagctgtat aaatcatcct tattggatga tcttctgaca gaatctgagg acatggcaca
3361 gcgcaggaaa gaagcagctg atatgctaaa ggcattacaa ggagccagtc aaattattgc
3421 tgaaatccgg gagactcatc tttggtgagg atccaccgga tctagataac tgatcataat
3481 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
3541 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
3601 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
3661 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta
3721 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
3781 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
3841 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
3901 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
3961 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
4021 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
4081 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
4141 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
4201 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
4261 aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa
4321 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
4381 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
4441 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
4501 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
4561 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
4621 aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt
4681 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
4741 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
4801 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
4861 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
4921 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
4981 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
5041 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
5101 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
5161 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc
5221 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
5281 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
5341 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
5401 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
5461 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
5521 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
5581 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
5641 cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac
5701 ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca
5761 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
5821 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg
5881 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata
5941 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
6001 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
6061 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
6121 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
6181 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
6241 agtgtagccg tagttaggcc accacttcaa gaactctgta tcaccgccta catacctcgc
6301 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
6361 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
6421 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
6481 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
6541 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
6601 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
6661 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
6721 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
6781 cgc
//
mChDrp1载体图谱质粒图谱pdf版和相关资料下载
mChDrp1载体质粒应用举例
- 上一篇：pNPTS138 Cm
- 下一篇：pHR CD19-PI3K CAR
