| 质粒名称: | pFLPo |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 13792 |
| 存储实验室: | Philippe Soriano |
|
载体骨架基本信息 |
|
| 载体名称: | na |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 2900bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | FLPo |
| 物种来源: | -- |
| 插入大小: | 1300 bp |
| 基因突变: | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AgeI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pFLPo was a gift from Philippe Soriano (Addgene plasmid # 13792 ; http://n2t.net/addgene:13792 ; RRID:Addgene_13792) |
| 参考文献部分: | High-Efficiency FLP and PhiC31 Site-Specific Recombination in Mammalian Cells. Raymond CS, Soriano P. PLoS ONE. 2007 . 2():e162. 10.1371/journal.pone.0000162 PubMed 17225864 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pFLPo 4161 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Expression of mouse codon-optimized FLP recombinase.
ACCESSION .
VERSION .
KEYWORDS pFLPo
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4161)
AUTHORS Raymond CS, Soriano P
TITLE High-Efficiency FLP and PhiC31 Site-Specific Recombination in
Mammalian Cells.
JOURNAL PLoS ONE. 2007 . 2():e162.
PUBMED 17225864
REFERENCE 2 (bases 1 to 4161)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4161
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin complement(3..458)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(140..161)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 352..371
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 585..607
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 599..616
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 600..616
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 626..645
/label=T7
/note="T7 promoter, forward primer"
promoter 626..644
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(1973..1993)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(1973..1991)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(2012..2028)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2012..2028)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(2025..2047)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 2036..2052
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(2060..2090)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2105..2126
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(2243..2260)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2414..3002)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2494..2513)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3173..4033)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 3796..3815
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4034..4138)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
181 caacgtcaaa gggcgaaaaa ccgtctatca gggctatggc ccactacgtg aaccatcacc
241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccga
661 tatcgccgcc accatgagcc agttcgacat cctgtgcaag acccccccca aggtgctggt
721 gcggcagttc gtggagagat tcgagaggcc cagcggcgag aagatcgcca gctgtgccgc
781 cgagctgacc tacctgtgct ggatgatcac ccacaacggc accgccatca agagggccac
841 cttcatgagc tacaacacca tcatcagcaa cagcctgagc ttcgacatcg tgaacaagag
901 cctgcagttc aagtacaaga cccagaaggc caccatcctg gaggccagcc tgaagaagct
961 gatccccgcc tgggagttca ccatcatccc ttacaacggc cagaagcacc agagcgacat
1021 caccgacatc gtgtccagcc tgcagctgca gttcgagagc agcgaggagg ccgacaaggg
1081 caacagccac agcaagaaga tgctgaaggc cctgctgtcc gagggcgaga gcatctggga
1141 gatcaccgag aagatcctga acagcttcga gtacaccagc aggttcacca agaccaagac
1201 cctgtaccag ttcctgttcc tggccacatt catcaactgc ggcaggttca gcgacatcaa
1261 gaacgtggac cccaagagct tcaagctggt gcagaacaag tacctgggcg tgatcattca
1321 gtgcctggtg accgagacca agacaagcgt gtccaggcac atctactttt tcagcgccag
1381 aggcaggatc gaccccctgg tgtacctgga cgagttcctg aggaacagcg agcccgtgct
1441 gaagagagtg aacaggaccg gcaacagcag cagcaacaag caggagtacc agctgctgaa
1501 ggacaacctg gtgcgcagct acaacaaggc cctgaagaag aacgccccct accccatctt
1561 cgctatcaag aacggcccta agagccacat cggcaggcac ctgatgacca gctttctgag
1621 catgaagggc ctgaccgagc tgacaaacgt ggtgggcaac tggagcgaca agagggcctc
1681 cgccgtggcc aggaccacct acacccacca gatcaccgcc atccccgacc actacttcgc
1741 cctggtgtcc aggtactacg cctacgaccc catcagcaag gagatgatcg ccctgaagga
1801 cgagaccaac cccatcgagg agtggcagca catcgagcag ctgaagggca gcgccgaggg
1861 cagcatcaga taccccgcct ggaacggcat catcagccag gaggtgctgg actacctgag
1921 cagctacatc aacaggcgga tctgatgaga tatcgagctc cagcttttgt tccctttagt
1981 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
2041 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
2101 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
2161 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
2221 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
2281 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
2341 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
2401 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
2461 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
2521 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
2581 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
2641 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
2701 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
2761 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
2821 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
2881 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
2941 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
3001 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
3061 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
3121 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
3181 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
3241 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
3301 caatgatacc gcgagaacca cgctcaccgg ctccagattt atcagcaata aaccagccag
3361 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
3421 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
3481 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
3541 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
3601 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
3661 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
3721 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
3781 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
3841 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
3901 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
3961 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
4021 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
4081 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
4141 catttccccg aaaagtgcca c
//
