pGL4.83[hRlucP/Puro]

价格:1500元

联系方式:I47-825O-882O

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pGL4.83载体基本信息

出品公司: Promega
载体名称: pGL4.83 ; pGL4.83[hRlucP/Puro]
质粒类型: 信号通路报告载体;哺乳动物载体;海肾荧光素酶报告载体
高拷贝/低拷贝: --
克隆方法: 限制性内切酶,多克隆位点
启动子:
载体大小: 4815 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: hRlucP
载体抗性: 氨苄青霉素
筛选标记: 嘌呤霉素(Puromycin)
克隆菌株: TOP10等常规菌株
宿主细胞(系): HEK293等
备注: pGL4.83[hRlucP/Puro]载体是信号通路报告载体;无启动子;含海肾荧光素酶报告基因hRlucP。
产品目录号: E7511
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

pGL4.83载体质粒图谱和多克隆位点信息

pGL4.83载体图谱



pGL4.83 多克隆位点

pGL4.83 载体特征

pGL4.83载体简介

The pGL4.83[hRlucP/Puro] Vector encodes the luciferase reporter gene hRlucP (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker for puromycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. It has also been engineered with fewer consensus regulatory sequences than the pGL3 Vectors and a synthetic reporter gene that has been codon optimized for mammalian expression. The pGL4.83[hRlucP/Puro] Vector is a basic vector with no promoter. However, it contains a multiple cloning region that allows cloning of a promoter of choice. The hRlucP reporter gene contains hPEST, a protein destabilization sequence. The protein encoded by hRlucP responds more quickly and with a greater magnitude to changes in transcriptional activity than the hRluc gene, its more stable counterpart.

pGL4.83载体序列

LOCUS       DQ188847                4815 bp    DNA     circular SYN 16-NOV-2005
DEFINITION  Synthetic Renilla luciferase reporter vector pGL4.83[hRlucP/Puro],
            complete sequence.
ACCESSION   DQ188847
VERSION     DQ188847.1  GI:76364318
KEYWORDS    .
SOURCE      Renilla luciferase reporter vector pGL4.83[hRlucP/Puro]
  ORGANISM  Renilla luciferase reporter vector pGL4.83[hRlucP/Puro]
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 4815)
  AUTHORS   Stecha,P., Paguio,A. and Chauvin,F.
  TITLE     pGL4 Luciferase Reporter Vectors Technical Manual, TM259, Promega
            Corporation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4815)
  AUTHORS   Stecha,P., Paguio,A. and Chauvin,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-2005) Scientific Communications, Promega
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
FEATURES             Location/Qualifiers
     source          1..4815
                     /organism="Renilla luciferase reporter vector
                     pGL4.83[hRlucP/Puro]"
                     /mol_type="other DNA"
                     /db_xref="taxon:346094"
     misc_feature    1..70
                     /note="multiple cloning region"
     CDS             100..1158
                     /note="synthetic Renilla luciferase; contains hPEST
                     destabilization sequence"
                     /codon_start=1
                     /product="hRlucP"
                     /protein_id="ABA41683.1"
                     /db_xref="GI:76364319"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAE
                     NAVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLT
                     AWFELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIE
                     EDIALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLS
                     WPREIPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNT
                     EFVKVKGLHFSQEDAPDEMGKYIKSFVERVLKNEQNSHGFPPEVEEQAAGTLPMSCAQ
                     ESGMDRHPAACASARINV"
     regulatory      1198..1419
                     /regulatory_class="polyA_signal_sequence"
                     /note="SV40 late poly(A) signal"
     regulatory      1467..1885
                     /regulatory_class="promoter"
                     /note="SV40 early enhancer/promoter"
     CDS             1910..2509
                     /note="puromycin resistance"
                     /codon_start=1
                     /product="puromycin-N-acetyltransferase"
                     /protein_id="ABA41684.1"
                     /db_xref="GI:76364320"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSR
                     LAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFL
                     ETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     regulatory      2534..2582
                     /regulatory_class="polyA_signal_sequence"
                     /note="synthetic poly(A) signal"
     primer_bind     2649..2668
                     /note="Reporter Vector primer 4 (RVprimer4) binding
                     region"
     rep_origin      2906
                     /note="ColEI-derived plasmid replication origin"
     gene            complement(3697..4557)
                     /gene="bla"
     CDS             complement(3697..4557)
                     /gene="bla"
                     /note="ampicillin resistance"
                     /codon_start=1
                     /product="beta-lactamase"
                     /protein_id="ABA41685.1"
                     /db_xref="GI:76364321"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     regulatory      3697..4557
                     /regulatory_class="polyA_signal_sequence"
                     /note="synthetic poly(A) signal/transcriptional pause
                     site"
     primer_bind     4764..4783
                     /note="Reporter Vector primer 3 (RVprimer3) binding
                     region"
ORIGIN      
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggcttccaa ggtgtacgac
      121 cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg caagcaaatg
      181 aacgtgctgg actccttcat caactactat gattccgaga agcacgccga gaacgccgtg
      241 atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc
      301 gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc cggcaagagc
      361 gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg gttcgagctg
      421 ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg tctggccttt
      481 cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga gagtgtcgtg
      541 gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc cctgatcaag
      601 agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac catgctccca
      661 agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga gccattcaag
      721 gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc tctcgttaag
      781 ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct tcgggccagc
      841 gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa cgctattgtc
      901 gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct ccacttcagc
      961 caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg
     1021 aagaacgagc agaattctca cggcttccct cccgaggtgg aggagcaggc cgccggcacc
     1081 ctgcccatga gctgcgccca ggagagcggc atggatagac accctgctgc ttgcgccagc
     1141 gccaggatca acgtctaagg ccgcgactct agagtcgggg cggccggccg cttcgagcag
     1201 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     1261 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     1321 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     1381 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgtttg
     1441 cgtattgggc gctcttccgc tgatctgcgc agcaccatgg cctgaaataa cctctgaaag
     1501 aggaacttgg ttagctacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
     1561 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     1621 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     1681 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     1741 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     1801 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     1861 aggcctaggc ttttgcaaaa agctcgattc ttctgacact agcgccacca tgaccgagta
     1921 caagcctacc gtgcgcctgg ccactcgcga tgatgtgccc cgcgccgtcc gcactctggc
     1981 cgccgctttc gccgactacc ccgctacccg gcacaccgtg gaccccgacc ggcacatcga
     2041 gcgtgtgaca gagttgcagg agctgttcct gacccgcgtc gggctggaca tcggcaaggt
     2101 gtgggtagcc gacgacggcg cggccgtggc cgtgtggact acccccgaga gcgttgaggc
     2161 cggcgccgtg ttcgccgaga tcggcccccg aatggccgag ctgagcggca gccgcctggc
     2221 cgcccagcag caaatggagg gcctgcttgc cccccatcgt cccaaggagc ctgcctggtt
     2281 tctggccact gtaggagtga gccccgacca ccagggcaag ggcttgggca gcgccgtcgt
     2341 gttgcccggc gtagaggccg ccgaacgcgc cggtgtgccc gcctttctcg aaacaagcgc
     2401 accaagaaac cttccattct acgagcgcct gggcttcacc gtgaccgccg atgtcgaggt
     2461 gcccgaggga cctaggacct ggtgtatgac acgaaaacct ggcgcctaat gatctagaac
     2521 cggtcatggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg
     2581 tgttcgaact agatgctgtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt
     2641 ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca
     2701 actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac tcgctgcgct
     2761 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     2821 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     2881 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     2941 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     3001 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     3061 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     3121 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     3181 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     3241 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     3301 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     3361 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     3421 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     3481 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     3541 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     3601 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     3661 ctgacagcgg ccgcaaatgc taaaccactg cagtggttac cagtgcttga tcagtgaggc
     3721 accgatctca gcgatctgcc tatttcgttc gtccatagtg gcctgactcc ccgtcgtgta
     3781 gatcactacg attcgtgagg gcttaccatc aggccccagc gcagcaatga tgccgcgaga
     3841 gccgcgttca ccggcccccg atttgtcagc aatgaaccag ccagcaggga gggccgagcg
     3901 aagaagtggt cctgctactt tgtccgcctc catccagtct atgagctgct gtcgtgatgc
     3961 tagagtaaga agttcgccag tgagtagttt ccgaagagtt gtggccattg ctactggcat
     4021 cgtggtatca cgctcgtcgt tcggtatggc ttcgttcaac tctggttccc agcggtcaag
     4081 ccgggtcaca tgatcaccca tattatgaag aaatgcagtc agctccttag ggcctccgat
     4141 cgttgtcaga agtaagttgg ccgcggtgtt gtcgctcatg gtaatggcag cactacacaa
     4201 ttctcttacc gtcatgccat ccgtaagatg cttttccgtg accggcgagt actcaaccaa
     4261 gtcgttttgt gagtagtgta tacggcgacc aagctgctct tgcccggcgt ctatacggga
     4321 caacaccgcg ccacatagca gtactttgaa agtgctcatc atcgggaatc gttcttcggg
     4381 gcggaaagac tcaaggatct tgccgctatt gagatccagt tcgatatagc ccactcttgc
     4441 acccagttga tcttcagcat cttttacttt caccagcgtt tcggggtgtg caaaaacagg
     4501 caagcaaaat gccgcaaaga agggaatgag tgcgacacga aaatgttgga tgctcatact
     4561 cgtccttttt caatattatt gaagcattta tcagggttac tagtacgtct ctcaaggata
     4621 agtaagtaat attaaggtac gggaggtatt ggacaggccg caataaaata tctttatttt
     4681 cattacatct gtgtgttggt tttttgtgtg aatcgatagt actaacatac gctctccatc
     4741 aaaacaaaac gaaacaaaac aaactagcaa aataggctgt ccccagtgca agtgcaggtg
     4801 ccagaacatt tctct
//

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