| 出品公司: | Agilent |
|---|---|
| 载体名称: | pFB-Luc |
| 质粒类型: | 逆病毒载体 |
| 高拷贝/低拷贝: | 低拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | -- |
| 载体大小: | 6768 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 无 |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 逆转录病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
pFB-Luc载体限制性酶切位点
pFB-Luc, 6768 bp version 013001
Enzymes with 1-10 cleavage sites:
#sites -- Bp position of recognition site --
AatII 2 978, 6694
Acc65I 3 647, 1846, 4294
AccI 3 1967, 2057, 4654
AccIII 2 2759, 3275
AflII 3 202, 1265, 3849
AflIII 3 164, 2557, 4883
AgeI 1 1840
AhdI 6 687, 733, 1274, 4334, 4380
5771
Alw44I 4 1054, 4699, 5197, 6443
AlwNI 5 323, 398, 3970, 4045, 5294
ApaI 1 1238
ApoI 5 87, 1128, 2467, 2651, 2905
AvaI 10 577, 610, 643, 1241, 2018
3120, 3725, 4224, 4257, 4290
BamHI 1 3720
BanII 8 580, 593, 764, 1238, 3084
4227, 4240, 4411
BbeI 4 615, 1656, 2096, 4262
BbsI 4 2067, 3345, 3475, 6761
BbvCI 2 453, 4100
BciVI 8 656, 1986, 2762, 3067, 3191
4303, 5092, 6619
BglI 1 5890
BglII 1 1678
BpmI 5 1799, 2128, 3322, 3797, 5861
Bpu10I 7 337, 412, 453, 1548, 3984
4059, 4100
BsaAI 4 1816, 2000, 2216, 4635
BsaHI 9 615, 978, 1656, 2070, 2096
3489, 4262, 6312, 6694
BsaI 9 694, 715, 782, 1414, 1802
4341, 4362, 4429, 5843
BsaWI 9 138, 1840, 2759, 3258, 3275
3642, 5089, 5236, 6067
BseRI 6 718, 1068, 1561, 1600, 3604
4365
BsiEI 9 961, 1034, 1838, 3145, 3731
4796, 5220, 6143, 6292
BsiHKAI 8 580, 1054, 3525, 4227, 4699
5197, 6358, 6443
BsiWI 1 2218
BsmBI 6 976, 1093, 1337, 1396, 1582
4532
Bsp120I 1 1238
BspHI 3 5603, 6611, 6716
BspLU11I 2 164, 4883
BspMI 1 3471
BsrBI 5 1299, 3728, 3753, 4814, 6615
BsrDI 2 5830, 6012
BsrGI 2 1541, 2554
BssHII 2 563, 4210
BssSI 3 5056, 6440, 6747
Bst1107I 1 4654
BstAPI 5 322, 397, 3969, 4044, 4701
BstBI 2 2232, 3020
BstEII 2 1346, 2671
Bsu36I 2 1276, 2676
Cfr10I 4 1840, 2333, 3492, 5856
ClaI 1 3428
DraI 3 5640, 5659, 6351
DraIII 1 1873
DrdI 2 4572, 4985
DsaI 3 151, 931, 2062
EaeI 7 827, 961, 1368, 1389, 1668
3731, 6164
EarI 7 1334, 1564, 2153, 2317, 2871
4761, 6565
Ecl136II 2 580, 4227
Eco52I 2 961, 3731
Eco57I 3 2892, 5410, 6458
Eco72I 1 2000
EcoNI 2 1647, 3677
EcoO109I 6 499, 1477, 1924, 3242, 4146
6751
EcoRI 1 2651
EcoRV 5 308, 383, 3400, 3955, 4030
EheI 4 615, 1656, 2096, 4262
FspI 1 5996
HaeII 6 615, 1656, 2096, 4262, 4757
5127
HincII 3 2057, 3366, 6315
KasI 4 615, 1656, 2096, 4262
KpnI 3 647, 1846, 4294
MscI 3 827, 1368, 1668
MslI 4 159, 6024, 6183, 6542
MunI 4 11, 21, 2179, 2635
NarI 4 615, 1656, 2096, 4262
NcoI 1 2062
NdeI 3 1664, 1672, 4705
NheI 5 6, 16, 26, 197, 3844
NotI 1 3730
NspI 4 164, 2723, 4518, 4883
PacI 1 3384
PpuMI 5 499, 1477, 1924, 3242, 4146
PshAI 1 1015
Psp1406I 2 6001, 6374
PstI 3 1178, 1360, 6017
PvuI 2 1034, 6143
PvuII 4 286, 361, 3933, 4008
SacI 2 580, 4227
SacII 1 151
SalI 1 2057
SapI 2 2871, 4760
ScaI 1 6254
SexAI 1 1474
SfcI 7 182, 1178, 1360, 3829, 5148
5339, 6017
SgrAI 1 3491
SmaI 4 643, 1241, 2018, 4290
SmlI 8 202, 1265, 3725, 3849, 4989
5251, 5528, 6396
SpeI 1 897
SphI 1 2723
SrfI 1 1240
SspI 1 6578
StyI 6 496, 706, 1504, 2062, 4143
4353
TatI 4 1541, 2554, 4689, 6254
TfiI 5 2293, 2520, 2716, 3426, 4858
Tsp45I 8 1282, 1491, 2565, 2626, 4538
4633, 6033, 6244
Tth111I 4 633, 1473, 4280, 4627
VspI 1 5947
XbaI 4 464, 2036, 2111, 4111
XcmI 1 2792
XhoI 1 3725
XmaI 4 643, 1241, 2018, 4290
XmnI 1 6371
Enzymes that do NOT cut molecule:
AscI BclI BlnI Bpu1102I BsaBI
BsgI BsmI BstXI Eco47III FseI
HindIII HpaI MluI NaeI NgoMIV
NruI NsiI PmeI Ppu10I RsrII
SanDI SbfI SfiI SgfI SnaBI
StuI SwaI Van91I
载体序列
pFB-Luc, 6768 bp version 013001 NOTE: The following sequence has been verified for accuracy at the junctions. The remainder of the sequence has been obtained from existing data. 1 GAATTGCTAG CAATTGCTAG CAATTGCTAG CAATTCATAC CAGATCACCG 51 AAAACTGTCC TCCAAATGTG TCCCCCTCAC ACTCCCAAAT TCGCGGGCTT 101 CTGCCTCTTA GACCACTCTA CCCTATTCCC CACACTCACC GGAGCCAAAG 151 CCGCGGGACA TATACATGTG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA 201 GCTTAAGTAA CGCCATTTTG CAAGGCATGG AAAAATACAT AACTGAGAAT 251 AGAAAAGTTC AGATCAAGGT CAGGAACAGA TGGAACAGCT GAATATGGGC 301 CAAAGCGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA 351 ACAGATGGAA CAGCTGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG 401 TTCCTGCCCC GGCTCAGGGC CAAGAACAGA TGGTCCCCAG ATGCGGTCCA 451 GCCCTCAGCA GTTTCTAGAG AACCATCAGA TGTTTCCAGG GTGCCCCAAG 501 GACCTGAAAT GACCCTGTGC CTTATTTGAA CTAACCAATC AGTTCGCTTC 551 TCGCTTCTGT TCGCGCGCTT CTGCTCCCCG AGCTCAATAA AAGAGCCCAC 601 AACCCCTCAC TCGGGGCGCC AGTCCTCCGA TTGACTGAGT CGCCCGGGTA 651 CCCGTGTATC CAATAAACCC TCTTGCAGTT GCATCCGACT TGTGGTCTCG 701 CTGTTCCTTG GGAGGGTCTC CTCTGAGTGA TTGACTACCC GTCAGCGGGG 751 GTCTTTCATT TGGGGGCTCG TCCGGGATCG GGAGACCCCT GCCCAGGGAC 801 CACCGACCCA CCACCGGGAG GTAAGCTGGC CAGCAACTTA TCTGTGTCTG 851 TCCGATTGTC TAGTGTCTAT GACTGATTTT ATGCGCCTGC GTCGGTACTA 901 GTTAGCTAAC TAGCTCTGTA TCTGGCGGAC CCGTGGTGGA ACTGACGAGT 951 TCGGAACACC CGGCCGCAAC CCTGGGAGAC GTCCCAGGGA CTTCGGGGGC 1001 CGTTTTTGTG GCCCGACCTG AGTCCAAAAA TCCCGATCGT TTTGGACTCT 1051 TTGGTGCACC CCCCTTAGAG GAGGGATATG TGGTTCTGGT AGGAGACGAG 1101 AACCTAAAAC AGTTCCCGCC TCCGTCTGAA TTTTTGCTTT CGGTTTGGGA 1151 CCGAAGCCGC GCCGCGCGTC TTGTCTGCTG CAGCATCGTT CTGTGTTGTC 1201 TCTGTCTGAC TGTGTTTCTG TATTTGTCTG AAAATATGGG CCCGGGCCAG 1251 ACTGTTACCA CTCCCTTAAG TTTGACCTTA GGTCACTGGA AAGATGTCGA 1301 GCGGATCGCT CACAACCAGT CGGTAGATGT CAAGAAGAGA CGTTGGGTTA 1351 CCTTCTGCTC TGCAGAATGG CCAACCTTTA ACGTCGGATG GCCGCGAGAC 1401 GGCACCTTTA ACCGAGACCT CATCACCCAG GTTAAGATCA AGGTCTTTTC 1451 ACCTGGCCCG CATGGACACC CAGACCAGGT CCCCTACATC GTGACCTGGG 1501 AAGCCTTGGC TTTTGACCCC CCTCCCTGGG TCAAGCCCTT TGTACACCCT 1551 AAGCCTCCGC CTCCTCTTCC TCCATCCGCC CCGTCTCTCC CCCTTGAACC 1601 TCCTCGTTCG ACCCCGCCTC GATCCTCCCT TTATCCAGCC CTCACTCCTT 1651 CTCTAGGCGC CCCCATATGG CCATATGAGA TCTTATATGG GGCACCCCCG 1701 CCCCTTGTAA ACTTCCCTGA CCCTGACATG ACAAGAGTTA CTAACAGCCC 1751 CTCTCTCCAA GCTCACTTAC AGGCTCTCTA CTTAGTCCAG CACGAAGTCT 1801 GGAGACCTCT GGCGGCACGT ACCAAGAACA ACTGGACCGA CCGGTGGTAC 1851 CTCACCCTTA CCGAGTCGGC GACACAGTGT GGGTCCGCCG ACACCAGACT 1901 AAGAACCTAG AACCTCGCTG GAAAGGACCT TACACAGTCC TGCTGACCAC 1951 CCCCACCGCC CTCAAAGTAG ACGGCATCGC AGCTTGGATA CACGCCGCCC 2001 ACGTGAAGGC TGCCGACCCC GGGGGTGGAC CATCCTCTAG ACTGCCGGAT 2051 CGAATTGTCG ACCATGGAAG ACGCCAAAAA CATAAAGAAA GGCCCGGCGC 2101 CATTCTATCC TCTAGAGGAT GGAACCGCTG GAGAGCAACT GCATAAGGCT 2151 ATGAAGAGAT ACGCCCTGGT TCCTGGAACA ATTGCTTTTA CAGATGCACA 2201 TATCGAGGTG AACATCACGT ACGCGGAATA CTTCGAAATG TCCGTTCGGT 2251 TGGCAGAAGC TATGAAACGA TATGGGCTGA ATACAAATCA CAGAATCGTC 2301 GTATGCAGTG AAAACTCTCT TCAATTCTTT ATGCCGGTGT TGGGCGCGTT 2351 ATTTATCGGA GTTGCAGTTG CGCCCGCGAA CGACATTTAT AATGAACGTG 2401 AATTGCTCAA CAGTATGAAC ATTTCGCAGC CTACCGTAGT GTTTGTTTCC 2451 AAAAAGGGGT TGCAAAAAAT TTTGAACGTG CAAAAAAAAT TACCAATAAT 2501 CCAGAAAATT ATTATCATGG ATTCTAAAAC GGATTACCAG GGATTTCAGT 2551 CGATGTACAC GTTCGTCACA TCTCATCTAC CTCCCGGTTT TAATGAATAC 2601 GATTTTGTAC CAGAGTCCTT TGATCGTGAC AAAACAATTG CACTGATAAT 2651 GAATTCCTCT GGATCTACTG GGTTACCTAA GGGTGTGGCC CTTCCGCATA 2701 GAACTGCCTG CGTCAGATTC TCGCATGCCA GAGATCCTAT TTTTGGCAAT 2751 CAAATCATTC CGGATACTGC GATTTTAAGT GTTGTTCCAT TCCATCACGG 2801 TTTTGGAATG TTTACTACAC TCGGATATTT GATATGTGGA TTTCGAGTCG 2851 TCTTAATGTA TAGATTTGAA GAAGAGCTGT TTTTACGATC CCTTCAGGAT 2901 TACAAAATTC AAAGTGCGTT GCTAGTACCA ACCCTATTTT CATTCTTCGC 2951 CAAAAGCACT CTGATTGACA AATACGATTT ATCTAATTTA CACGAAATTG 3001 CTTCTGGGGG CGCACCTCTT TCGAAAGAAG TCGGGGAAGC GGTTGCAAAA 3051 CGCTTCCATC TTCCAGGGAT ACGACAAGGA TATGGGCTCA CTGAGACTAC 3101 ATCAGCTATT CTGATTACAC CCGAGGGGGA TGATAAACCG GGCGCGGTCG 3151 GTAAAGTTGT TCCATTTTTT GAAGCGAAGG TTGTGGATCT GGATACCGGG 3201 AAAACGCTGG GCGTTAATCA GAGAGGCGAA TTATGTGTCA GAGGACCTAT 3251 GATTATGTCC GGTTATGTAA ACAATCCGGA AGCGACCAAC GCCTTGATTG 3301 ACAAGGATGG ATGGCTACAT TCTGGAGACA TAGCTTACTG GGACGAAGAC 3351 GAACACTTCT TCATAGTTGA CCGCTTGAAG TCTTTAATTA AATACAAAGG 3401 ATATCAGGTG GCCCCCGCTG AATTGGAATC GATATTGTTA CAACACCCCA 3451 ACATCTTCGA CGCGGGCGTG GCAGGTCTTC CCGACGATGA CGCCGGTGAA 3501 CTTCCCGCCG CCGTTGTTGT TTTGGAGCAC GGAAAGACGA TGACGGAAAA 3551 AGAGATCGTG GATTACGTCG CCAGTCAAGT AACAACCGCG AAAAAGTTGC 3601 GCGGAGGAGT TGTGTTTGTG GACGAAGTAC CGAAAGGTCT TACCGGAAAA 3651 CTCGACGCAA GAAAAATCAG AGAGATCCTC ATAAAGGCCA AGAAGGGCGG 3701 AAAGTCCAAA TTGTAATGAG GATCCTCGAG CGGCCGCGAT CCGAGTTCTT 3751 CTGAGCGGGA CTCTGGGGTT CGATAAAATA AAAGATTTTA TTTAGTCTCC 3801 AGAAAAAGGG GGGAATGAAA GACCCCACCT GTAGGTTTGG CAAGCTAGCT 3851 TAAGTAACGC CATTTTGCAA GGCATGGAAA AATACATAAC TGAGAATAGA 3901 GAAGTTCAGA TCAAGGTCAG GAACAGATGG AACAGCTGAA TATGGGCCAA 3951 ACAGGATATC TGTGGTAAGC AGTTCCTGCC CCGGCTCAGG GCCAAGAACA 4001 GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC 4051 CTGCCCCGGC TCAGGGCCAA GAACAGATGG TCCCCAGATG CGGTCCAGCC 4101 CTCAGCAGTT TCTAGAGAAC CATCAGATGT TTCCAGGGTG CCCCAAGGAC 4151 CTGAAATGAC CCTGTGCCTT ATTTGAACTA ACCAATCAGT TCGCTTCTCG 4201 CTTCTGTTCG CGCGCTTCTG CTCCCCGAGC TCAATAAAAG AGCCCACAAC 4251 CCCTCACTCG GGGCGCCAGT CCTCCGATTG ACTGAGTCGC CCGGGTACCC 4301 GTGTATCCAA TAAACCCTCT TGCAGTTGCA TCCGACTTGT GGTCTCGCTG 4351 TTCCTTGGGA GGGTCTCCTC TGAGTGATTG ACTACCCGTC AGCGGGGGTC 4401 TTTCATTTGG GGGCTCGTCC GGGATCGGGA GACCCCTGCC CAGGGACCAC 4451 CGACCCACCA CCGGGAGGTA AGCTGGCTGC CTCGCGCGTT TCGGTGATGA 4501 CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC 4551 TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT 4601 GTTGGCGGGT GTCGGGGCGC AGCCATGACC CAGTCACGTA GCGATAGCGG 4651 AGTGTATACT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT 4701 GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC 4751 GCATCAGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC 4801 GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 4851 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC 4901 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT 4951 AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 5001 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 5051 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG 5101 TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 5151 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 5201 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT 5251 CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 5301 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 5351 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC 5401 TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 5451 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC 5501 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT 5551 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 5601 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA 5651 AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC 5701 AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT 5751 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC 5801 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA 5851 CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC 5901 CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA 5951 ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC 6001 AACGTTGTTG CCATTGCTGC AGGCATCGTG GTGTCACGCT CGTCGTTTGG 6051 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT 6101 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT 6151 GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT 6201 GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG 6251 GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT 6301 TGCTCTTGCC CGGCGTCAAC ACGGGATAAT ACCGCGCCAC ATAGCAGAAC 6351 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA 6401 GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC 6451 AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA 6501 AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT 6551 GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 6601 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA 6651 ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCT 6701 AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG 6751 AGGCCCTTTC GTCTTCAA
