pFB-Luc

价格:3000元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Agilent
载体名称: pFB-Luc
质粒类型: 逆病毒载体
高拷贝/低拷贝: 低拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: --
载体大小: 6768 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素
筛选标记:
克隆菌株: DH5α 等
宿主细胞(系): 常规细胞系(293、CV-1、CHO等)
备注: --
产品目录号: --
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 逆转录病毒

载体质粒图谱和多克隆位点信息

pFB-Luc载体图谱

载体简介

pFB-Luc载体限制性酶切位点

pFB-Luc, 6768 bp			     version 013001


Enzymes with 1-10 cleavage sites:

            #sites   --  Bp position of recognition site --

  AatII          2      978,   6694
  Acc65I         3      647,   1846,   4294
  AccI           3     1967,   2057,   4654
  AccIII         2     2759,   3275
  AflII          3      202,   1265,   3849
  AflIII         3      164,   2557,   4883
  AgeI           1     1840
  AhdI           6      687,    733,   1274,   4334,   4380
                       5771
  Alw44I         4     1054,   4699,   5197,   6443
  AlwNI          5      323,    398,   3970,   4045,   5294
  ApaI           1     1238
  ApoI           5       87,   1128,   2467,   2651,   2905
  AvaI          10      577,    610,    643,   1241,   2018
                       3120,   3725,   4224,   4257,   4290
  BamHI          1     3720
  BanII          8      580,    593,    764,   1238,   3084
                       4227,   4240,   4411
  BbeI           4      615,   1656,   2096,   4262
  BbsI           4     2067,   3345,   3475,   6761
  BbvCI          2      453,   4100
  BciVI          8      656,   1986,   2762,   3067,   3191
                       4303,   5092,   6619
  BglI           1     5890
  BglII          1     1678
  BpmI           5     1799,   2128,   3322,   3797,   5861
  Bpu10I         7      337,    412,    453,   1548,   3984
                       4059,   4100
  BsaAI          4     1816,   2000,   2216,   4635
  BsaHI          9      615,    978,   1656,   2070,   2096
                       3489,   4262,   6312,   6694
  BsaI           9      694,    715,    782,   1414,   1802
                       4341,   4362,   4429,   5843
  BsaWI          9      138,   1840,   2759,   3258,   3275
                       3642,   5089,   5236,   6067
  BseRI          6      718,   1068,   1561,   1600,   3604
                       4365
  BsiEI          9      961,   1034,   1838,   3145,   3731
                       4796,   5220,   6143,   6292
  BsiHKAI        8      580,   1054,   3525,   4227,   4699
                       5197,   6358,   6443
  BsiWI          1     2218
  BsmBI          6      976,   1093,   1337,   1396,   1582
                       4532
  Bsp120I        1     1238
  BspHI          3     5603,   6611,   6716
  BspLU11I       2      164,   4883
  BspMI          1     3471
  BsrBI          5     1299,   3728,   3753,   4814,   6615
  BsrDI          2     5830,   6012
  BsrGI          2     1541,   2554
  BssHII         2      563,   4210
  BssSI          3     5056,   6440,   6747
  Bst1107I       1     4654
  BstAPI         5      322,    397,   3969,   4044,   4701
  BstBI          2     2232,   3020
  BstEII         2     1346,   2671
  Bsu36I         2     1276,   2676
  Cfr10I         4     1840,   2333,   3492,   5856
  ClaI           1     3428
  DraI           3     5640,   5659,   6351
  DraIII         1     1873
  DrdI           2     4572,   4985
  DsaI           3      151,    931,   2062
  EaeI           7      827,    961,   1368,   1389,   1668
                       3731,   6164
  EarI           7     1334,   1564,   2153,   2317,   2871
                       4761,   6565
  Ecl136II       2      580,   4227
  Eco52I         2      961,   3731
  Eco57I         3     2892,   5410,   6458
  Eco72I         1     2000
  EcoNI          2     1647,   3677
  EcoO109I       6      499,   1477,   1924,   3242,   4146
                       6751
  EcoRI          1     2651
  EcoRV          5      308,    383,   3400,   3955,   4030
  EheI           4      615,   1656,   2096,   4262
  FspI           1     5996
  HaeII          6      615,   1656,   2096,   4262,   4757
                       5127
  HincII         3     2057,   3366,   6315
  KasI           4      615,   1656,   2096,   4262
  KpnI           3      647,   1846,   4294
  MscI           3      827,   1368,   1668
  MslI           4      159,   6024,   6183,   6542
  MunI           4       11,     21,   2179,   2635
  NarI           4      615,   1656,   2096,   4262
  NcoI           1     2062
  NdeI           3     1664,   1672,   4705
  NheI           5        6,     16,     26,    197,   3844
  NotI           1     3730
  NspI           4      164,   2723,   4518,   4883
  PacI           1     3384
  PpuMI          5      499,   1477,   1924,   3242,   4146
  PshAI          1     1015
  Psp1406I       2     6001,   6374
  PstI           3     1178,   1360,   6017
  PvuI           2     1034,   6143
  PvuII          4      286,    361,   3933,   4008
  SacI           2      580,   4227
  SacII          1      151
  SalI           1     2057
  SapI           2     2871,   4760
  ScaI           1     6254
  SexAI          1     1474
  SfcI           7      182,   1178,   1360,   3829,   5148
                       5339,   6017
  SgrAI          1     3491
  SmaI           4      643,   1241,   2018,   4290
  SmlI           8      202,   1265,   3725,   3849,   4989
                       5251,   5528,   6396
  SpeI           1      897
  SphI           1     2723
  SrfI           1     1240
  SspI           1     6578
  StyI           6      496,    706,   1504,   2062,   4143
                       4353
  TatI           4     1541,   2554,   4689,   6254
  TfiI           5     2293,   2520,   2716,   3426,   4858
  Tsp45I         8     1282,   1491,   2565,   2626,   4538
                       4633,   6033,   6244
  Tth111I        4      633,   1473,   4280,   4627
  VspI           1     5947
  XbaI           4      464,   2036,   2111,   4111
  XcmI           1     2792
  XhoI           1     3725
  XmaI           4      643,   1241,   2018,   4290
  XmnI           1     6371





Enzymes that do NOT cut molecule:

AscI        BclI        BlnI        Bpu1102I    BsaBI       
BsgI        BsmI        BstXI       Eco47III    FseI        
HindIII     HpaI        MluI        NaeI        NgoMIV      
NruI        NsiI        PmeI        Ppu10I      RsrII       
SanDI       SbfI        SfiI        SgfI        SnaBI       
StuI        SwaI        Van91I      



载体序列

pFB-Luc, 6768 bp                              version 013001

NOTE: The following sequence has been verified for accuracy
at the junctions. The remainder of the sequence has been 
obtained from existing data. 

   1  GAATTGCTAG CAATTGCTAG CAATTGCTAG CAATTCATAC CAGATCACCG  
  51  AAAACTGTCC TCCAAATGTG TCCCCCTCAC ACTCCCAAAT TCGCGGGCTT  
 101  CTGCCTCTTA GACCACTCTA CCCTATTCCC CACACTCACC GGAGCCAAAG  
 151  CCGCGGGACA TATACATGTG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA  
 201  GCTTAAGTAA CGCCATTTTG CAAGGCATGG AAAAATACAT AACTGAGAAT  
 251  AGAAAAGTTC AGATCAAGGT CAGGAACAGA TGGAACAGCT GAATATGGGC  
 301  CAAAGCGGAT ATCTGTGGTA AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA  
 351  ACAGATGGAA CAGCTGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG  
 401  TTCCTGCCCC GGCTCAGGGC CAAGAACAGA TGGTCCCCAG ATGCGGTCCA  
 451  GCCCTCAGCA GTTTCTAGAG AACCATCAGA TGTTTCCAGG GTGCCCCAAG  
 501  GACCTGAAAT GACCCTGTGC CTTATTTGAA CTAACCAATC AGTTCGCTTC  
 551  TCGCTTCTGT TCGCGCGCTT CTGCTCCCCG AGCTCAATAA AAGAGCCCAC  
 601  AACCCCTCAC TCGGGGCGCC AGTCCTCCGA TTGACTGAGT CGCCCGGGTA  
 651  CCCGTGTATC CAATAAACCC TCTTGCAGTT GCATCCGACT TGTGGTCTCG  
 701  CTGTTCCTTG GGAGGGTCTC CTCTGAGTGA TTGACTACCC GTCAGCGGGG  
 751  GTCTTTCATT TGGGGGCTCG TCCGGGATCG GGAGACCCCT GCCCAGGGAC  
 801  CACCGACCCA CCACCGGGAG GTAAGCTGGC CAGCAACTTA TCTGTGTCTG  
 851  TCCGATTGTC TAGTGTCTAT GACTGATTTT ATGCGCCTGC GTCGGTACTA  
 901  GTTAGCTAAC TAGCTCTGTA TCTGGCGGAC CCGTGGTGGA ACTGACGAGT  
 951  TCGGAACACC CGGCCGCAAC CCTGGGAGAC GTCCCAGGGA CTTCGGGGGC  
1001  CGTTTTTGTG GCCCGACCTG AGTCCAAAAA TCCCGATCGT TTTGGACTCT  
1051  TTGGTGCACC CCCCTTAGAG GAGGGATATG TGGTTCTGGT AGGAGACGAG  
1101  AACCTAAAAC AGTTCCCGCC TCCGTCTGAA TTTTTGCTTT CGGTTTGGGA  
1151  CCGAAGCCGC GCCGCGCGTC TTGTCTGCTG CAGCATCGTT CTGTGTTGTC  
1201  TCTGTCTGAC TGTGTTTCTG TATTTGTCTG AAAATATGGG CCCGGGCCAG  
1251  ACTGTTACCA CTCCCTTAAG TTTGACCTTA GGTCACTGGA AAGATGTCGA  
1301  GCGGATCGCT CACAACCAGT CGGTAGATGT CAAGAAGAGA CGTTGGGTTA  
1351  CCTTCTGCTC TGCAGAATGG CCAACCTTTA ACGTCGGATG GCCGCGAGAC  
1401  GGCACCTTTA ACCGAGACCT CATCACCCAG GTTAAGATCA AGGTCTTTTC  
1451  ACCTGGCCCG CATGGACACC CAGACCAGGT CCCCTACATC GTGACCTGGG  
1501  AAGCCTTGGC TTTTGACCCC CCTCCCTGGG TCAAGCCCTT TGTACACCCT  
1551  AAGCCTCCGC CTCCTCTTCC TCCATCCGCC CCGTCTCTCC CCCTTGAACC  
1601  TCCTCGTTCG ACCCCGCCTC GATCCTCCCT TTATCCAGCC CTCACTCCTT  
1651  CTCTAGGCGC CCCCATATGG CCATATGAGA TCTTATATGG GGCACCCCCG  
1701  CCCCTTGTAA ACTTCCCTGA CCCTGACATG ACAAGAGTTA CTAACAGCCC  
1751  CTCTCTCCAA GCTCACTTAC AGGCTCTCTA CTTAGTCCAG CACGAAGTCT  
1801  GGAGACCTCT GGCGGCACGT ACCAAGAACA ACTGGACCGA CCGGTGGTAC  
1851  CTCACCCTTA CCGAGTCGGC GACACAGTGT GGGTCCGCCG ACACCAGACT  
1901  AAGAACCTAG AACCTCGCTG GAAAGGACCT TACACAGTCC TGCTGACCAC  
1951  CCCCACCGCC CTCAAAGTAG ACGGCATCGC AGCTTGGATA CACGCCGCCC  
2001  ACGTGAAGGC TGCCGACCCC GGGGGTGGAC CATCCTCTAG ACTGCCGGAT  
2051  CGAATTGTCG ACCATGGAAG ACGCCAAAAA CATAAAGAAA GGCCCGGCGC  
2101  CATTCTATCC TCTAGAGGAT GGAACCGCTG GAGAGCAACT GCATAAGGCT  
2151  ATGAAGAGAT ACGCCCTGGT TCCTGGAACA ATTGCTTTTA CAGATGCACA  
2201  TATCGAGGTG AACATCACGT ACGCGGAATA CTTCGAAATG TCCGTTCGGT  
2251  TGGCAGAAGC TATGAAACGA TATGGGCTGA ATACAAATCA CAGAATCGTC  
2301  GTATGCAGTG AAAACTCTCT TCAATTCTTT ATGCCGGTGT TGGGCGCGTT  
2351  ATTTATCGGA GTTGCAGTTG CGCCCGCGAA CGACATTTAT AATGAACGTG  
2401  AATTGCTCAA CAGTATGAAC ATTTCGCAGC CTACCGTAGT GTTTGTTTCC  
2451  AAAAAGGGGT TGCAAAAAAT TTTGAACGTG CAAAAAAAAT TACCAATAAT  
2501  CCAGAAAATT ATTATCATGG ATTCTAAAAC GGATTACCAG GGATTTCAGT  
2551  CGATGTACAC GTTCGTCACA TCTCATCTAC CTCCCGGTTT TAATGAATAC  
2601  GATTTTGTAC CAGAGTCCTT TGATCGTGAC AAAACAATTG CACTGATAAT  
2651  GAATTCCTCT GGATCTACTG GGTTACCTAA GGGTGTGGCC CTTCCGCATA  
2701  GAACTGCCTG CGTCAGATTC TCGCATGCCA GAGATCCTAT TTTTGGCAAT  
2751  CAAATCATTC CGGATACTGC GATTTTAAGT GTTGTTCCAT TCCATCACGG  
2801  TTTTGGAATG TTTACTACAC TCGGATATTT GATATGTGGA TTTCGAGTCG  
2851  TCTTAATGTA TAGATTTGAA GAAGAGCTGT TTTTACGATC CCTTCAGGAT  
2901  TACAAAATTC AAAGTGCGTT GCTAGTACCA ACCCTATTTT CATTCTTCGC  
2951  CAAAAGCACT CTGATTGACA AATACGATTT ATCTAATTTA CACGAAATTG  
3001  CTTCTGGGGG CGCACCTCTT TCGAAAGAAG TCGGGGAAGC GGTTGCAAAA  
3051  CGCTTCCATC TTCCAGGGAT ACGACAAGGA TATGGGCTCA CTGAGACTAC  
3101  ATCAGCTATT CTGATTACAC CCGAGGGGGA TGATAAACCG GGCGCGGTCG  
3151  GTAAAGTTGT TCCATTTTTT GAAGCGAAGG TTGTGGATCT GGATACCGGG  
3201  AAAACGCTGG GCGTTAATCA GAGAGGCGAA TTATGTGTCA GAGGACCTAT  
3251  GATTATGTCC GGTTATGTAA ACAATCCGGA AGCGACCAAC GCCTTGATTG  
3301  ACAAGGATGG ATGGCTACAT TCTGGAGACA TAGCTTACTG GGACGAAGAC  
3351  GAACACTTCT TCATAGTTGA CCGCTTGAAG TCTTTAATTA AATACAAAGG  
3401  ATATCAGGTG GCCCCCGCTG AATTGGAATC GATATTGTTA CAACACCCCA  
3451  ACATCTTCGA CGCGGGCGTG GCAGGTCTTC CCGACGATGA CGCCGGTGAA  
3501  CTTCCCGCCG CCGTTGTTGT TTTGGAGCAC GGAAAGACGA TGACGGAAAA  
3551  AGAGATCGTG GATTACGTCG CCAGTCAAGT AACAACCGCG AAAAAGTTGC  
3601  GCGGAGGAGT TGTGTTTGTG GACGAAGTAC CGAAAGGTCT TACCGGAAAA  
3651  CTCGACGCAA GAAAAATCAG AGAGATCCTC ATAAAGGCCA AGAAGGGCGG  
3701  AAAGTCCAAA TTGTAATGAG GATCCTCGAG CGGCCGCGAT CCGAGTTCTT  
3751  CTGAGCGGGA CTCTGGGGTT CGATAAAATA AAAGATTTTA TTTAGTCTCC  
3801  AGAAAAAGGG GGGAATGAAA GACCCCACCT GTAGGTTTGG CAAGCTAGCT  
3851  TAAGTAACGC CATTTTGCAA GGCATGGAAA AATACATAAC TGAGAATAGA  
3901  GAAGTTCAGA TCAAGGTCAG GAACAGATGG AACAGCTGAA TATGGGCCAA  
3951  ACAGGATATC TGTGGTAAGC AGTTCCTGCC CCGGCTCAGG GCCAAGAACA  
4001  GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC  
4051  CTGCCCCGGC TCAGGGCCAA GAACAGATGG TCCCCAGATG CGGTCCAGCC  
4101  CTCAGCAGTT TCTAGAGAAC CATCAGATGT TTCCAGGGTG CCCCAAGGAC  
4151  CTGAAATGAC CCTGTGCCTT ATTTGAACTA ACCAATCAGT TCGCTTCTCG  
4201  CTTCTGTTCG CGCGCTTCTG CTCCCCGAGC TCAATAAAAG AGCCCACAAC  
4251  CCCTCACTCG GGGCGCCAGT CCTCCGATTG ACTGAGTCGC CCGGGTACCC  
4301  GTGTATCCAA TAAACCCTCT TGCAGTTGCA TCCGACTTGT GGTCTCGCTG  
4351  TTCCTTGGGA GGGTCTCCTC TGAGTGATTG ACTACCCGTC AGCGGGGGTC  
4401  TTTCATTTGG GGGCTCGTCC GGGATCGGGA GACCCCTGCC CAGGGACCAC  
4451  CGACCCACCA CCGGGAGGTA AGCTGGCTGC CTCGCGCGTT TCGGTGATGA  
4501  CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC  
4551  TGTAAGCGGA TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT  
4601  GTTGGCGGGT GTCGGGGCGC AGCCATGACC CAGTCACGTA GCGATAGCGG  
4651  AGTGTATACT GGCTTAACTA TGCGGCATCA GAGCAGATTG TACTGAGAGT  
4701  GCACCATATG CGGTGTGAAA TACCGCACAG ATGCGTAAGG AGAAAATACC  
4751  GCATCAGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC  
4801  GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT  
4851  ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC  
4901  AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT  
4951  AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG  
5001  GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA  
5051  GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG  
5101  TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG  
5151  TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC  
5201  ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT  
5251  CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC  
5301  TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT  
5351  TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC  
5401  TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG  
5451  ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC  
5501  AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT  
5551  TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT  
5601  GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA  
5651  AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC  
5701  AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT  
5751  TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC  
5801  GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA  
5851  CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC  
5901  CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA  
5951  ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC  
6001  AACGTTGTTG CCATTGCTGC AGGCATCGTG GTGTCACGCT CGTCGTTTGG  
6051  TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT  
6101  CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT  
6151  GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT  
6201  GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG  
6251  GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT  
6301  TGCTCTTGCC CGGCGTCAAC ACGGGATAAT ACCGCGCCAC ATAGCAGAAC  
6351  TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA  
6401  GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC  
6451  AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA  
6501  AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT  
6551  GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG  
6601  GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA  
6651  ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCT  
6701  AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG  
6751  AGGCCCTTTC GTCTTCAA

载体质粒图谱pdf版和相关资料下载

载体应用举例

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