| 质粒名称: | pAAVLINK-EF1a-HA-5'-TCOF1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001659 |
| 产品用途: | 5' AAVLINK plasmid for TCOF1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | TCOF1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-TCOF1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-TCOF1载体质粒基本信息
pAAVLINK-EF1a-HA-5'-TCOF1质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-TCOF1载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-TCOF1质粒载体简介
pAAVLINK-EF1a-HA-5'-TCOF1质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-TCOF1 6213 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6213)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 1, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6213
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 158..700
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..3353
/codon_start=1
/label=TCOF1
/translation="MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVT
LLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPR
LASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEP
SANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQ
VKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEE
EAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSE
SSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVA
KAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRK
TGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPL
GKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNIL
QAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPA
LKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSS
SSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQ
VKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKV
VTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDS"
misc_feature 1017..1022
/label=SDS
misc_feature 1269..1274
/label=SDS
misc_feature 1272..1277
/label=SDS
misc_feature 1507..1512
/label=SDS
misc_feature 1569..1574
/label=SDS
misc_feature 1587..1592
/label=SDS
misc_feature 1590..1595
/label=SDS
misc_feature 1755..1760
/label=SDS
misc_feature 1797..1802
/label=SDS
misc_feature 1968..1973
/label=SDS
misc_feature 2007..2012
/label=SDS
misc_feature 2010..2015
/label=SDS
misc_feature 2111..2116
/label=SDS
misc_feature 2160..2165
/label=SDS
misc_feature 2163..2168
/label=SDS
misc_feature 2373..2378
/label=SDS
misc_feature 2610..2615
/label=SDS
misc_feature 3045..3050
/label=SDS
misc_feature 3342..3347
/label=SDS
intron 3354..3394
/label=Splicing Donor
misc_feature 3398..3431
/label=lox2272-JT1571
repeat_region 3476..3616
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3476..3605
/label=AAV2 ITR
rep_origin 3691..4146
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4428..4532
/gene="bla"
/label=AmpR promoter
CDS 4533..5393
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5564..6152
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctat ggccgaggcc aggaagcggc gggagctact
781 tcccctgatc taccaccatc tgctgcgggc tggctatgtg cgtgcggcgc gggaagtgaa
841 ggagcagagc ggccagaagt gtttcctggc tcagcccgta acccttctgg acatctatac
901 acactggcaa caaacctcag agcttggtcg gaagcggaag gcagaggaag atgcggcact
961 gcaagctaag aaaacccgtg tgtcagaccc catcagcacc tcggagagct cggaagagga
1021 ggaagaagca gaagccgaaa ccgccaaagc caccccaaga ctagcatcta ccaactcctc
1081 agtcctgggg gcggacttgc catcaagcat gaaagaaaaa gccaaggcag agacagagaa
1141 agctggcaag actgggaatt ccatgccaca ccctgccact gggaagacgg tggccaacct
1201 tctttctggg aagtctccca ggaagtcagc agagccctca gcaaatacta cgttggtctc
1261 agaaactgag gaggagggca gcgtcccggc ctttggagct gctgccaagc ctgggatggt
1321 gtcagcgggc caggccgaca gctccagcga ggacacctcc agctccagtg atgagacaga
1381 cgtggagggg aaaccctcag taaaaccagc ccaggtcaaa gcctcatcag tttctactaa
1441 ggagtctcca gcaagaaagg cggccccagc ccctgggaag gtgggggatg tgacacccca
1501 ggtcaaagga ggggccctgc ccccagccaa gagggccaag aagccagaag aggagtcaga
1561 gagtagtgag gagggatctg aaagtgagga ggaggcccct gcagggacac gaagccaggt
1621 aaaggcctct gaaaaaattc tccaggtcag agctgcctca gcccctgcca aggggacccc
1681 tgggaaaggg gctaccccag caccccctgg gaaggcaggg gctgtagcct cccagaccaa
1741 ggcagggaag ccagaggagg actcagagag cagcagcgag gagtcatctg acagtgagga
1801 ggagacgcca gctgccaagg ccctgcttca ggcgaaggcc tcaggaaaaa cctctcaggt
1861 cggagctgcc tcagcccctg ccaaggagtc ccccaggaaa ggagctgccc cagcgccccc
1921 tgggaagaca gggcctgcag ttgccaaggc ccaggcgggg aagcgggagg aggactcgca
1981 gagcagcagc gaggaatcgg acagtgagga ggaggcgcct gctcaggcga agccttcagg
2041 gaaggccccc caggtcagag ccgcctcggc ccctgccaag gagtccccca ggaaaggggc
2101 tgccccagca cctcctagga aaacagggcc tgcagccgcc caggtccagg tggggaagca
2161 ggaggaggac tcaagaagca gcagcgagga gtcagacagt gacagagagg cactggcagc
2221 catgaatgca gctcaggtga agcccttggg gaaaagcccc caggtgaaac ctgcctctac
2281 catgggcatg gggcccttgg ggaaaggcgc cggcccagtg ccacccggga aggtggggcc
2341 tgcaaccccc tcagcccagg tggggaagtg ggaggaggac tcagagagca gtagtgagga
2401 gtcatcagac agcagtgatg gagaggtgcc cacagctgtg gccccggctc aggaaaagtc
2461 cttggggaac atcctccagg ccaaacccac ctccagtcct gccaaggggc cccctcagaa
2521 ggcagggcct gtagccgtcc aggtcaaggc tgaaaagccc atggacaact cggagagcag
2581 cgaggagtca tcggacagtg cggacagtga ggaggcacca gcagccatga ctgcagctca
2641 ggcaaaacca gctctgaaaa ttcctcagac caaggcctgc ccaaagaaaa ccaataccac
2701 tgcatctgcc aaggtcgccc ctgtgcgagt gggcacccaa gccccccgga aagcaggaac
2761 tgcgacttct ccagcaggct catccccagc tgtggctggg ggcacccaga gaccagcaga
2821 ggattcttca agcagtgagg aatcagatag tgaggaagag aagacaggtc ttgcagtaac
2881 cgtgggacag gcaaagtctg tggggaaagg cctccaggtg aaagcagcct cagtgcctgt
2941 caaggggtcc ttggggcaag ggactgctcc agtactccct gggaagacgg ggcctacagt
3001 cacccaggtg aaagctgaaa agcaggaaga ctctgagagc agtgaggagg aatcagacag
3061 tgaggaagca gctgcatctc cagcacaggt gaaaacctca gtaaagaaaa cccaggccaa
3121 agccaaccca gctgccgcca gagcaccttc agcaaaaggg acaatttcag cccctggaaa
3181 agttgtcact gcagctgctc aagccaagca gaggtctcca tccaaggtga agccaccagt
3241 gagaaacccc cagaacagta ccgtcttggc gaggggccca gcatctgtgc catctgtggg
3301 gaaggccgtg gctacagcag ctcaggccca gacagggcca gaggaggact caggtaagta
3361 tcaaggttac aagacaggtt taaggagacc aataagctat tattcgtata ggatacttta
3421 tacgaagtta ttaagttatg gagaccggtg tggcacgtgc ggaccgagcg gccgcaggaa
3481 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
3541 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
3601 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
3661 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
3721 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc
3781 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
3841 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
3901 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
3961 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
4021 actctatctc gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt
4081 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
4141 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
4201 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
4261 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
4321 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
4381 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
4441 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
4501 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
4561 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
4621 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
4681 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
4741 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
4801 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
4861 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
4921 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
4981 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
5041 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
5101 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
5161 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
5221 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
5281 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
5341 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
5401 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
5461 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
5521 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
5581 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
5641 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
5701 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
5761 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
5821 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
5881 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
5941 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
6001 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
6061 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
6121 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
6181 tcctggcctt ttgctggcct tttgctcaca tgt
//
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pAAVLINK-EF1a-HA-5'-TCOF1质粒载体应用举例
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