| 质粒名称: | pUDP004 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 101165 |
| 存储实验室: | Jean-Marc Daran |
|
载体骨架基本信息 |
|
| 载体名称: | not applicable |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 酵母表达,CRISPR |
| 筛选标记: | amdS（赋予乙酰胺生长的乙酰胺酶基因） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | ScTDH3 |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gibson 克隆 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pUDP004 was a gift from Jean-Marc Daran (Addgene plasmid # 101165 ; http://n2t.net/addgene:101165 ; RRID:Addgene_101165) |
| 参考文献部分: | CRISPR-Cas9 mediated gene deletions in lager yeast Saccharomyces pastorianus. de Vries ARG, de Groot PA, van den Broek M, Daran JG. Microb Cell Fact. 2017 Dec 5;16(1):222. doi: 10.1186/s12934-017-0835-1. 10.1186/s12934-017-0835-1 [pii] PubMed 29207996 |
买家导航
pUDP004载体质粒基本信息
pUDP004质粒图谱载体图谱和pUDP004载体序列质粒序列多克隆位点信息
pUDP004质粒载体简介
pUDP004质粒序列载体序列
LOCUS pUDP004 10664 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION E. coli/S. cerevisiae shuttle vector carrying amd S marker and
Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for
Cas9 editing (HH-gRNA-HDV).
ACCESSION .
VERSION .
KEYWORDS pUDP004
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10664)
AUTHORS de Vries ARG, de Groot PA, van den Broek M, Daran JG
TITLE CRISPR-Cas9 mediated gene deletions in lager yeast Saccharomyces
pastorianus.
JOURNAL Microb Cell Fact. 2017 Dec 5;16(1):222. doi:
10.1186/s12934-017-0835-1.
PUBMED 29207996
REFERENCE 2 (bases 1 to 10664)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10664
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 2574..2597
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 2601..2621
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 2622..6722
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTYKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSITHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 6726..6746
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
promoter 7665..8331
/gene="S. cerevisiae TDH3"
/label=GAP promoter
/note="promoter for glyceraldehyde-3-phosphate
dehydrogenase"
primer_bind 8201..8224
/label=GPDpro-F
/note="S. cerevisiae GPD promoter, forward primer"
primer_bind complement(8393..8411)
/label=CYC1
/note="CYC1 transcription termination signal, reverse
primer"
CDS complement(8845..9705)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGTRVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9468..9487
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9706..9810)
/gene="bla"
/label=AmpR promoter
rep_origin 9913..10501
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 10402..10421
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 gacatggagg cccagaatac cctccttgac agtcttgacg tgcgcagctc aggggcatga
61 tgtgactgtc gcccgtacat ttagcccata catccccatg tataatcatt tgcatccata
121 cattttgatg gccgcacggc gcgaagcaaa aattacggct cctcgctgca gacctgcgag
181 cagggaaacg ctcccctcac agacgcgttg aattgtcccc acgccgcgcc cctgtagaga
241 aatataaaag gttaggattt gccactgagg ttcttctttc atatacttcc ttttaaaatc
301 ttgctaggat acagttctca catcacatcc gaacataaac aaggatccat gccacaatct
361 tgggaagaat tggctgctga caagagagct agattggcta agactatccc agacgaatgg
421 aaggttcaaa ctttgccagc tgaagactct gttatcgact tcccaaagaa gtctggtatc
481 ttgtctgaag ctgaattgaa gatcactgaa gcttctgctg ctgacttggt ttctaagttg
541 gctgctggtg aattgacttc tgttgaagtt actttggctt tctgtaagag agctgctatc
601 gctcaacaat tgactaactg tgctcacgaa ttcttcccag acgctgcttt ggctcaagct
661 agagaattgg acgaatacta cgctaagcac aaaagacctg ttggtccatt gcacggtttg
721 ccaatctctt tgaaggacca attgagagtt aagggttacg aaacttctat gggttacatc
781 tcttggttga acaagtacga cgaaggtgac tctgttttga ctactatgtt gagaaaggct
841 ggtgctgttt tctacgttaa gacttctgtt ccacaaactt tgatggtttg tgaaactgtt
901 aacaacatca tcggtagaac tgttaaccca agaaacaaga actggtcttg tggtggttct
961 tctggtggtg aaggtgctat cgttggtatc agaggtggtg ttatcggtgt tggtactgac
1021 atcggtggtt ctatcagagt tccagctgct ttcaacttct tgtacggttt aagaccatct
1081 cacggtagat tgccatacgc taagatggct aactctatgg aaggtcaaga aactgtacac
1141 tctgttgttg gtccaatcac tcactctgtt gaagacttga gattgttcac taagtctgtt
1201 ttgggtcaag aaccatggaa gtacgactct aaggttatcc caatgccatg gagacaatct
1261 gaatctgaca tcatcgcttc taagatcaag aacggtggtt tgaacatcgg ttactacaac
1321 ttcgacggta acgttttgcc acacccacca atcttgagag gtgttgaaac tactgttgct
1381 gctttggcta aggctggtca cactgttact ccatggactc catacaagca cgacttcggt
1441 cacgacttga tctctcacat ctacgctgct gacggttctg ctgacgttat gagagacatc
1501 tctgcttctg gtgaaccagc tatcccaaac atcaaggact tgttgaaccc aaacatcaag
1561 gctgttaaca tgaacgaatt gtgggacact cacttgcaaa agtggaacta ccaaatggaa
1621 tacttggaaa agtggagaga agctgaagaa aaggctggta aggaattgga cgctatcatc
1681 gctccaatca ctccaactgc tgctgttaga cacgaccaat tcagatacta cggttacgct
1741 tctgttatca acttgttgga cttcacttct gttgttgttc cagttacttt cgctgacaag
1801 aacatcgaca agaagaacga atctttcaag gctgtttctg aattggacgc tttggttcaa
1861 gaagaatacg acccagaagc ttaccacggt gctccagttg ctgttcaagt tatcggtaga
1921 agattgtctg aagaaagaac tttggctatc gctgaagaag ttggtaagtt gttgggtaac
1981 gttgttactc cataactcga ggacaataaa aagattcttg ttttcaagaa cttgtcattt
2041 gtatagtttt tttatattgt agttgttcta ttttaatcaa atgttagcgt gatttatatt
2101 ttttttcgcc tcgacatcat ctgcccagat gcgaagttaa gtgcgcagaa agtaatatca
2161 tgcgtcaatc gtatgtgaat gctggtcgct atactggagc tctcgagaac cctcaccttt
2221 cgagaggacg atgcccgtgt ctaaatgatt cgaccagcct aagaatgttc aacaatctat
2281 aatcagtcca tagtcaacaa gagccctcaa agttgatatc gtttagtgat ctccctcgag
2341 atgagcggag gatggttgct ttttggggag ccctaagtgg tacgtgcggc gagcgggatg
2401 tttccccatt aggcaaagga cgaccgggta acccctcgag aaaaaaaatt tttcgccgct
2461 aatctggtgt tatataaagc tccccctgct cctggaattt ttttccttgt caactcacac
2521 cggaaatcaa ggcatttcat tctgagtagt tcttaaaaac ataatcaaca atggattata
2581 aagatgacga tgacaaacct ccaaaaaaga agagaaaggt cgataagaaa tactcaatag
2641 gcttagatat cggcacaaat agcgtcggat gggcggtgat cactgatgaa tataaggttc
2701 cgtctaaaaa gttcaaggtt ctgggaaata cagaccgcca cagtatcaaa aaaaatctta
2761 taggggctct tttatttgac agtggagaga cagcggaagc gactcgtctc aaacggacag
2821 ctcgtagaag gtatacacgt cggaagaatc gtatttgtta tctacaggag attttttcaa
2881 atgagatggc gaaagtagat gatagtttct ttcatcgact tgaagagtct tttttggtgg
2941 aagaagacaa gaagcatgaa cgtcatccta tttttggaaa tatagtagat gaagttgctt
3001 atcatgagaa atatccaact atctatcatc tgcgaaaaaa attggtagat tctacttata
3061 aagcggattt gcgcttaatc tatttggcct tagcgcatat gattaagttt cgtggtcatt
3121 ttttgattga gggagattta aatcctgata atagtgatgt ggacaaacta tttatccagt
3181 tggtacaaac ctacaatcaa ttatttgaag aaaaccctat taacgcaagt ggagtagatg
3241 ctaaagcgat tctttctgca cgattgagta aatcaagacg attagaaaat ctcattgctc
3301 agctccccgg tgagaagaaa aatggcttat ttgggaatct cattgctttg tcattgggtt
3361 tgacccctaa ttttaaatca aattttgatt tggcagaaga tgctaaatta cagctttcaa
3421 aagatactta cgatgatgat ttagataatt tattggcgca aattggagat caatatgctg
3481 atttgttttt ggcagctaag aatttatcag atgctatttt actttcagat atcctaagag
3541 taaatactga aataactaag gctcccctat cagcttcaat gattaaacgc tacgatgaac
3601 atcatcaaga cttgactctt ttaaaagctt tagttcgaca acaacttcca gaaaagtata
3661 aagaaatctt ttttgatcaa tcaaaaaacg gatatgcagg ttatattgat gggggagcta
3721 gccaagaaga attttataaa tttatcaaac caattttaga aaaaatggat ggtactgagg
3781 aattattggt gaaactaaat cgtgaagatt tgctgcgcaa gcaacggacc tttgacaacg
3841 gctctattac ccatcaaatt cacttgggtg agctgcatgc tattttgaga agacaagaag
3901 acttttatcc atttttaaaa gacaatcgtg agaagattga aaaaatcttg acttttcgaa
3961 ttccttatta tgttggtcca ttggcgcgtg gcaatagtcg ttttgcatgg atgactcgga
4021 agtctgaaga aacaattacc ccatggaatt ttgaagaagt tgtcgataaa ggtgcttcag
4081 ctcaatcatt tattgaacgc atgacaaact ttgataaaaa tcttccaaat gaaaaagtac
4141 taccaaaaca tagtttgctt tatgagtatt ttacggttta taacgaattg acaaaggtca
4201 aatatgttac tgaaggaatg cgaaaaccag catttctttc aggtgaacag aagaaagcca
4261 ttgttgattt actcttcaaa acaaatcgaa aagtaaccgt taagcaatta aaagaagatt
4321 atttcaaaaa aatagaatgt tttgatagtg ttgaaatttc aggagttgaa gatagattta
4381 atgcttcatt aggtacctac catgatttgc taaaaattat taaagataaa gattttttgg
4441 ataatgaaga aaatgaagat atcttagagg atattgtttt aacattgacc ttatttgaag
4501 atagggagat gattgaggaa agacttaaaa catatgctca cctctttgat gataaggtga
4561 tgaaacagct taaacgtcgc cgttatactg gttggggacg tttgtctcga aaattgatta
4621 atggtattag ggataagcaa tctggcaaaa caatattaga ttttttgaaa tcagatggtt
4681 ttgccaatcg caattttatg cagctgatcc atgatgatag tttgacattt aaagaagaca
4741 ttcaaaaagc acaagtgtct ggacaaggcg atagtttaca tgaacatatt gcaaatttag
4801 ctggtagccc tgctattaaa aaaggtattt tacagactgt aaaagttgtt gatgaattgg
4861 tcaaagtaat ggggcggcat aagccagaaa atatcgttat tgaaatggca cgtgaaaatc
4921 agacaactca aaagggccag aaaaattcgc gagagcgtat gaaacgaatc gaagaaggta
4981 tcaaagaatt aggaagtcag attcttaaag agcatcctgt tgaaaatact caattgcaaa
5041 atgaaaagct ctatctctat tatctccaaa atggaagaga catgtatgtg gaccaagaat
5101 tagatattaa tcgtttaagt gattatgatg tcgatcacat tgttccacaa agtttcctta
5161 aagacgattc aatagacaat aaggtcttaa cgcgttctga taaaaatcgt ggtaaatcgg
5221 ataacgttcc aagtgaagaa gtagtcaaaa agatgaaaaa ctattggaga caacttctaa
5281 acgccaagtt aatcactcaa cgtaagtttg ataatttaac gaaagctgaa cgtggaggtt
5341 tgagtgaact tgataaagct ggttttatca aacgccaatt ggttgaaact cgccaaatca
5401 ctaagcatgt ggcacaaatt ttggatagtc gcatgaatac taaatacgat gaaaatgata
5461 aacttattcg agaggttaaa gtgattacct taaaatctaa attagtttct gacttccgaa
5521 aagatttcca attctataaa gtacgtgaga ttaacaatta ccatcatgcc catgatgcgt
5581 atctaaatgc cgtcgttgga actgctttga ttaagaaata tccaaaactt gaatcggagt
5641 ttgtctatgg tgattataaa gtttatgatg ttcgtaaaat gattgctaag tctgagcaag
5701 aaataggcaa agcaaccgca aaatatttct tttactctaa tatcatgaac ttcttcaaaa
5761 cagaaattac acttgcaaat ggagagattc gcaaacgccc tctaatcgaa actaatgggg
5821 aaactggaga aattgtctgg gataaagggc gagattttgc cacagtgcgc aaagtattgt
5881 ccatgcccca agtcaatatt gtcaagaaaa cagaagtaca gacaggcgga ttctccaagg
5941 agtcaatttt accaaaaaga aattcggaca agcttattgc tcgtaaaaaa gactgggatc
6001 caaaaaaata tggtggtttt gatagtccaa cggtagctta ttcagtccta gtggttgcta
6061 aggtggaaaa agggaaatcg aagaagttaa aatccgttaa agagttacta gggatcacaa
6121 ttatggaaag aagttccttt gaaaaaaatc cgattgactt tttagaagct aaaggatata
6181 aggaagttaa aaaagactta atcattaaac tacctaaata tagtcttttt gagttagaaa
6241 acggtcgtaa acggatgctg gctagtgccg gagaattaca aaaaggaaat gagctggctc
6301 tgccaagcaa atatgtgaat tttttatatt tagctagtca ttatgaaaag ttgaagggta
6361 gtccagaaga taacgaacaa aaacaattgt ttgtggagca gcataagcat tatttagatg
6421 agattattga gcaaatcagt gaattttcta agcgtgttat tttagcagat gccaatttag
6481 ataaagttct tagtgcatat aacaaacata gagacaaacc aatacgtgaa caagcagaaa
6541 atattattca tttatttacg ttgacgaatc ttggagctcc cgctgctttt aaatattttg
6601 atacaacaat tgatcgtaaa cgatatacgt ctacaaaaga agttttagat gccactctta
6661 tccatcaatc catcactggt ctttatgaaa cacgcattga tttgagtcag ctaggaggtg
6721 accctccaaa aaagaagaga aaggtctgag cggttttgta taactaaata atattggaaa
6781 ctaaatacga atacccaaat tttttatcta aattttgccg aaagattaaa atctgcagag
6841 atatccgaaa caggtaaatg gatgtttcaa tccctgtagt cagtcaggaa cccatattat
6901 attacagtat tagtcgccgc ttaggcacgc ctttaattag caaaatcaaa ccttaagtgc
6961 atatgccgta taagggaaac tcaaagaact ggcatcgcaa aaatgaaaac atacgttgaa
7021 actacggcaa aggattggtc agatcgcttc atacagggaa agttcggcat caacatcttt
7081 ggataatatc agaatgagaa agaacagata cgcagtacgt tttttggtga gctctttgca
7141 cttctttagt tctttccatc aatatcagtt ttttaaactt ttaggactaa aagtgatgtt
7201 taacttcaaa atgtttaaaa ttttgttctt cccgacgttc attaagaata ctaatacact
7261 ttaataatta ttttaatatt ttgttctaaa taatgacttt taattaaaaa agataaaata
7321 taaaaacatc ataataactc accagaggtt aagaacaaaa aaacaaatta gatatctgct
7381 aatccaatat agttaaatca atctttcctt ggtataatgg gtatattaca tatatttcaa
7441 ggaccgacac tcctaccaaa tatctaaaat ttaccatatt aacataacat gtatataaac
7501 gtcaaatcat aatcagcact aacgtctcac ggatcgtata tgccgtagcg acaatctaag
7561 aactatgcga ggacacgcta gggccaaggc aaaaagattc cttgattacg taagggagtt
7621 agaatcattt tgaataaaaa acacgctttt tcagttcgag tttatcatta tcaatactgc
7681 catttcaaag aatacgtaaa taattaatag tagtgatttt cctaacttta tttagtcaaa
7741 aaattagcct tttaattctg ctgtaacccg tacatgccca aaataggggg cgggttacac
7801 agaatatata acatcgtagg tgtctgggtg aacagtttat tcttggcatc cactaaatat
7861 aatggagccc gctttttaag ctggcatcca gaaaaaaaaa gaatcccagc accaaaatat
7921 tgttttcttc accaaccatc agttcatagg tccattctct tagcgcaact acagagaaca
7981 ggggcacaaa caggcaaaaa acgggcacaa cctcaatgga gtgatgcaac ctgcctggag
8041 taaatgatga cacaaggcaa ttgacccacg catgtatcta tctcattttc ttacaccttc
8101 tattaccttc tgctctctct gatttggaaa aagctgaaaa aaaaggttga aaccagttcc
8161 ctgaaattat tcccctactt gactaataag tatataaaga cggtaggtat tgattgtaat
8221 tctgtaaatc tatttcttaa acttcttaaa ttctactttt atagttagtc ttttttttag
8281 ttttaaaaca ccaagaactt agtttcgaat aaacacacat aaacaaacaa acgagacctg
8341 cggaggtctc cacaggcccc ttttcctttg tcgatatcat gtaattagtt atgtcacgct
8401 tacattcacg ccctcccccc acatccgctc taaccgaaaa ggaaggagtt agacaacctg
8461 aagtctaggt ccctatttat ttttttatag ttatgttagt attaagaacg ttatttatat
8521 ttcaaatttt tctttttttt ctgtacaaac gcgtgtacgc atgtaacatt atactgaaaa
8581 ccttgcttga gaaggttttg ggacaagctt acgtctcacg gatcgtatat gccgtagcga
8641 caatctaaga actatgcgag gacacgctag cagctgtatt cacgtagacg gataggtata
8701 gccagacatc agcagcatac ttcgggaacc gtaggccacc gagcggccgc gattatcaaa
8761 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
8821 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
8881 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
8941 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgggacc cacgctcacc
9001 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
9061 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
9121 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
9181 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
9241 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
9301 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
9361 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
9421 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
9481 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
9541 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgtac ccaactgatc
9601 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
9661 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
9721 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
9781 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgtcat
9841 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
9901 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
9961 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
10021 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
10081 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
10141 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
10201 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
10261 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
10321 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
10381 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcc
10441 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
10501 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
10561 gttctttcct gcgttatccc ctgattctgt ggataaccgt gcggactata tgtgaaggca
10621 tggctatggc acggcagaca ttccgccaga tcatcaatag gcac
//
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pUDP004质粒载体应用举例
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