pCambia3301

价格:1500元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: CAMBIA
载体名称: pCAMBIA3301
质粒类型: 植物载体;农杆菌双元表达载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: lac
载体大小: 11319 bp
5' 测序引物及序列: M13 Reverse:CAGGAAACAGCTATGAC
3' 测序引物及序列: M13/pUC Forward:CCCAGTCACGACGTTGTAAAACG
载体标签: --
载体抗性: 卡那霉素
筛选标记: 草丁膦(磷丝菌素)phosphinothricin、GUS
克隆菌株: HB101等菌株
宿主细胞(系): 植物细胞、农杆菌
备注: --
产品目录号: --
稳定性: 稳表达
组成型/诱导型: 诱导型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pCAMBIA3301载体图谱



pCAMBIA3301 多克隆位点

载体简介

载体序列

LOCUS       pCAMBIA3301    11319 bp    DNA     circular    SYN    16-Feb-2014
DEFINITION  pCAMBIA3301
ACCESSION    
KEYWORDS    
SOURCE    
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..11319
                     /organism="pCAMBIA3301"
                     /mol_type="other DNA"
     CDS             2..16
                     /label="GUS\First\Exon"
                     /translation="MVDLR"
     misc_feature    17..206
                     /label="Catalase\Intron"
     misc_feature    324..343
                     /label="gusA176"
     misc_feature    complement(521..540)
                     /label="gusA-354"
     misc_feature    561..587
                     /label="gusA350"
     misc_feature    624..645
                     /label="gusA478"
     misc_feature    complement(815..834)
                     /label="gusA-648"
     misc_feature    927..946
                     /label="gusA779"
     CDS             207..2024
                     /label="gusA\Second\Exon"
     misc_feature    complement(1119..1138)
                     /label="gusA-952"
     misc_feature    1247..1266
                     /label="gusA1099"
     misc_feature    complement(1423..1442)
                     /label="gusA-1256"
     misc_feature    1554..1573
                     /label="gusA1403"
     misc_feature    complement(1556..1579)
                     /label="gusA-1400"
     misc_feature    complement(1721..1740)
                     /label="gusA-1551"
     misc_feature    1852..1871
                     /label="gusA1701"
     misc_feature    2086..2338
                     /label="NOS\polyA"
     misc_feature    2376..2401
                     /label="T-BORDER\(R)"
     misc_feature    complement(3442..4442)
                     /label="pVS1\Sta"
     rep_origin      complement(5035..6035)
                     /label="pVS1-REP"
     misc_feature    complement(6445..6705)
                     /label="pBR322\bom\site"
     rep_origin      complement(6845..7125)
                     /label="pBR322\ori"
     CDS             complement(7419..8210)
                     /label="kanamycin\(R)"
     misc_feature    8635..8660
                     /label="T\BORDER\(L)"
     misc_feature    8801..8808
                     /label="POLY\A\SITE"
     CDS             complement(8918..9469)
                     /label="bar"
     misc_feature    complement(9482..10289)
                     /label="CAMV35S"
     misc_feature    10534..10584
                     /label="MCS"
     CDS             10510..10744
                     /label="LAC\Z\ALPHA"
     promoter        10776..11313
                     /label="35S\promoter"
ORIGIN
    1 catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct
   61 tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga
  121 ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt
  181 gtctatgatg atgatgatag ttacagaacc gacgactcgt ccgtcctgta gaaaccccaa
  241 cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc gaaaactgtg
  301 gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag
  361 gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgcgggc aacgtctggt
  421 atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg ctgcgtttcg
  481 atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg
  541 gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac
  601 gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga
  661 ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg
  721 gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg
  781 tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca
  841 atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag
  901 gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc
  961 tctatgaact cgaagtcaca gccaaaagcc agacagagtc tgatatctac ccgcttcgcg
 1021 tcggcatccg gtcagtggca gtgaagggcc aacagttcct gattaaccac aaaccgttct
 1081 actttactgg ctttggtcgt catgaagatg cggacttacg tggcaaagga ttcgataacg
 1141 tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct
 1201 cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga
 1261 ttgatgaaac tgctgctgtc ggctttcagc tgtctttagg cattggtttc gaagcgggca
 1321 acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact
 1381 tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga
 1441 gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg
 1501 cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct
 1561 gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttatt
 1621 acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac
 1681 ttctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata
 1741 cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat
 1801 ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat
 1861 ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag
 1921 ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga
 1981 ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa acaagctagc caccaccacc
 2041 accaccacgt gtgaattaca ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg
 2101 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
 2161 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
 2221 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
 2281 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa
 2341 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
 2401 attagaataa cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg
 2461 tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg
 2521 ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca
 2581 caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc
 2641 gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa
 2701 caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt
 2761 gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat
 2821 caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg
 2881 cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga
 2941 cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc
 3001 cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt
 3061 cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg
 3121 cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct
 3181 gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc
 3241 gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg
 3301 cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga
 3361 acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt
 3421 accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac
 3481 gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc
 3541 tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta
 3601 tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa
 3661 caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag
 3721 gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc
 3781 tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc
 3841 aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg
 3901 gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg
 3961 cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg
 4021 ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac
 4081 aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg
 4141 aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct
 4201 acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg
 4261 cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg
 4321 taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag
 4381 cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt
 4441 tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac
 4501 cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat
 4561 aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag
 4621 gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg
 4681 gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt
 4741 gacggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg
 4801 gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc
 4861 ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca
 4921 gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt
 4981 ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc
 5041 cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg
 5101 cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta
 5161 ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac
 5221 aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc
 5281 gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac
 5341 gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt
 5401 gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc
 5461 gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg
 5521 ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc
 5581 ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa
 5641 cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg
 5701 tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta
 5761 gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag
 5821 cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg
 5881 gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg
 5941 aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa
 6001 aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg
 6061 gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt
 6121 cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc
 6181 tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg
 6241 ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg
 6301 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
 6361 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
 6421 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
 6481 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
 6541 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
 6601 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
 6661 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
 6721 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
 6781 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
 6841 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
 6901 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
 6961 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
 7021 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
 7081 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
 7141 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
 7201 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
 7261 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
 7321 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
 7381 tcacgttaag ggattttggt catgcattct aggtactaaa acaattcatc cagtaaaata
 7441 taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca
 7501 tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac
 7561 ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca
 7621 aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc
 7681 gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt
 7741 tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg
 7801 tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac
 7861 tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa
 7921 aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc
 7981 aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc
 8041 acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt
 8101 tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg
 8161 tatttttcga tcagtttttt caattccggt gatattctca ttttagccat ttattatttc
 8221 cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact
 8281 ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt
 8341 tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat
 8401 cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc
 8461 gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa
 8521 agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt
 8581 atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag
 8641 gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat tgcggacgtt
 8701 tttaatgtac tgaattaacg ccgaattaat tcgggggatc tggattttag tactggattt
 8761 tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta
 8821 agggtttctt atatgctcaa cacatgagcg aaaccctata ggaaccctaa ttcccttatc
 8881 tgggaactac tcacacatta ttatggagaa actcgagtca aatctcggtg acgggcagga
 8941 ccggacgggg cggtaccggc aggctgaagt ccagctgcca gaaacccacg tcatgccagt
 9001 tcccgtgctt gaagccggcc gcccgcagca tgccgcgggg ggcatatccg agcgcctcgt
 9061 gcatgcgcac gctcgggtcg ttgggcagcc cgatgacagc gaccacgctc ttgaagccct
 9121 gtgcctccag ggacttcagc aggtgggtgt agagcgtgga gcccagtccc gtccgctggt
 9181 ggcgggggga gacgtacacg gtcgactcgg ccgtccagtc gtaggcgttg cgtgccttcc
 9241 aggggcccgc gtaggcgatg ccggcgacct cgccgtccac ctcggcgacg agccagggat
 9301 agcgctcccg cagacggacg aggtcgtccg tccactcctg cggttcctgc ggctcggtac
 9361 ggaagttgac cgtgcttgtc tcgatgtagt ggttgacgat ggtgcagacc gccggcatgt
 9421 ccgcctcggt ggcacggcgg atgtcggccg ggcgtcgttc tgggctcatg gtagactcga
 9481 gagagataga tttgtagaga gagactggtg atttcagcgt gtcctctcca aatgaaatga
 9541 acttccttat atagaggaag gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg
 9601 tcagtggaga tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt
 9661 tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct
 9721 tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct
 9781 actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat
 9841 attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata
 9901 ttcttggagt agacgagagt gtcgtgctcc accatgttat cacatcaatc cacttgcttt
 9961 gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct
10021 ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc gcaatgatgg
10081 catttgtagg tgccaccttc cttttctact gtccttttga tgaagtgaca gatagctggg
10141 caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc aatagccctt
10201 tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc gtgctccacc
10261 atgttggcaa gctgctctag ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
10321 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
10381 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
10441 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
10501 attacgaatt cgagctcggt acccggggat cctctagagt cgacctgcag gcatgcaagc
10561 ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
10621 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
10681 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gctagagcag cttgagcttg
10741 gatcagattg tcgtttcccg ccttcagttt agcttcatgg agtcaaagat tcaaatagag
10801 gacctaacag aactcgccgt aaagactggc gaacagttca tacagagtct cttacgactc
10861 aatgacaaga agaaaatctt cgtcaacatg gtggagcacg acacacttgt ctactccaaa
10921 aatatcaaag atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta
10981 atatccggaa acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata
11041 gtggaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt
11101 gaagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
11161 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga tatctccact
11221 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
11281 agttcatttc atttggagag aacacggggg actcttgac
//

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