| 质粒名称: | TFORF2826 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 143631 |
| 存储实验室: | Feng Zhang |
|
载体骨架基本信息 |
|
| 载体名称: | pLX_TRC317 |
| 载体出品公司: | Broad Institute Genetic Perturbation Platform |
| 空载体大小: | 8264bp |
| 完整质粒大小: | 7981bp |
| 载体类型: | 哺乳细胞表达, Lentiviral |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | IKZF1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1232bp |
| GenBank编号 | JN247409 |
| 启动子: | EF1a |
| 标签/融合蛋白: | 人细胞色素c氧化酶亚基VIII（N端） |
| 克隆方法: | 未知 |
| 5’测序引物 | GGGTGGAGACTGAAGTTAGGCCAG |
| 3’测序引物 |
CACATAGCGTAAAAGGAGCAACATAG
(Common Sequencing Primers)
|
|
文献引用方法 |
|
| 材料和方法部分: | TFORF2826 was a gift from Feng Zhang (Addgene plasmid # 143631 ; http://n2t.net/addgene:143631 ; RRID:Addgene_143631) |
| 参考文献部分: | A transcription factor atlas of directed differentiation. Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F. Cell. 2023 Jan 5;186(1):209-229.e26. doi: 10.1016/j.cell.2022.11.026. 10.1016/j.cell.2022.11.026 PubMed 36608654 |
买家导航
TFORF2826载体质粒基本信息
TFORF2826质粒图谱载体图谱和TFORF2826载体序列质粒序列多克隆位点信息
TFORF2826质粒载体简介
TFORF2826质粒序列载体序列
LOCUS TFORF2826 9496 bp DNA circular SYN 15-APR-2025
DEFINITION Lentiviral vector for overexpressing transcription factor ORFs with
unique 24-bp barcodes. Barcodes facilitate identification of
transcription factors in pooled screens..
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9496)
AUTHORS Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine
VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
TITLE A transcription factor atlas of directed differentiation.
JOURNAL Cell. 2023 Jan 5;186(1):209-229.e26. doi:
10.1016/j.cell.2022.11.026.
PUBMED 36608654
REFERENCE 2 (bases 1 to 9496)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 15, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi:
"10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT Sequence Label: TFORF2826
FEATURES Location/Qualifiers
source 1..9496
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 5..231
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 232..412
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 459..584
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1077..1310
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1495..1539
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
promoter 1770..1980
/label=SV40 promoter
/note="SV40 early promoter"
promoter 1792..1988
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 1839..1974
/label=SV40 ori
/note="SV40 origin of replication"
CDS 2020..2622
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
primer_bind complement(2020..2039)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 2516..2536
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 2665..2782
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2920..4098
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 3151..4089
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 4046..4066
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
regulatory 4109..4118
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
misc_feature 5353..5941
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5406..5426)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5824..5835)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 6013..6246
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 6324..6445
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6361..6380)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6415..6434
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 6485..6620
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(6640..6659)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(6641..6659)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(6669..6686)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(6669..6685)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6678..6700)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 6827..7282
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6914..6933)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 7124..7145
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 7308..7412
/gene="bla"
/label=AmpR promoter
CDS join(7413..7481,7482..8273)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7631..7650)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 8444..9032
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8933..8952
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 9186..9203
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 9320..9341
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter join(9356..9361,9362..9379,9380..9386)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9394..9410
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 9399..9421
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 9418..9434
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 9418..9434
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 9453..9473
/label=T3
/note="T3 promoter, forward primer"
promoter 9455..9473
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggat gctgatgagg gtcaagacat
4141 gtcccaagtt tcagggaagg aaagcccccc tgtaagcgat actccagatg agggcgatga
4201 gcccatgccg atccccgagg acctctccac cacctcggga ggacagcaaa gctccaagag
4261 tgacagagtc gtggccagta atgttaaagt agagactcag agtgatgaag agaatgggcg
4321 tgcctgtgaa atgaatgggg aagaatgtgc ggaggattta cgaatgcttg atgcctcggg
4381 agagaaaatg aatggctccc acagggacca aggcagctcg gctttgtcgg gagttggagg
4441 cattcgactt cctaacggaa aactaaagtg tgatatctgt gggatcattt gcatcgggcc
4501 caatgtgctc atggttcaca aaagaagcca cactggggac aagggcctgt ccgacacgcc
4561 ctacgacagc agcgccagct acgagaagga gaacgaaatg atgaagtccc acgtgatgga
4621 ccaagccatc aacaacgcca tcaactacct gggggccgag tccctgcgcc cgctggtgca
4681 gacgcccccg ggcggttccg aggtggtccc ggtcatcagc ccgatgtacc agctgcacaa
4741 gccgctcgcg gagggcaccc cgcgctccaa ccactcggcc caggacagcg ccgtggagaa
4801 cctgctgctg ctctccaagg ccaagttggt gccctcggag cgcgaggcgt ccccgagcaa
4861 cagctgccaa gactccacgg acaccgagag caacaacgag gagcagcgca gcggtctcat
4921 ctacctgacc aaccacatcg ccccgcacgc gcgcaacggg ctgtcgctca aggaggagca
4981 ccgcgcctac gacctgctgc gcgccgcctc cgagaactcg caggacgcgc tccgcgtggt
5041 cagcaccagc ggggagcaga tgaaggtgta caagtgcgaa cactgccggg tgctcttcct
5101 ggatcacgtc atgtacacca tccacatggg ctgccacggc ttccgtgatc cttttgagtg
5161 caacatgtgc ggctaccaca gccaggaccg gtacgagttc tcgtcgcaca taacgcgagg
5221 ggagcaccgc ttccacatga gctagtaatg aactagtccg taacttgaaa gtatttcgat
5281 ttcttggctt tatatatctt gtggaaagga cgacggagta tccttccatg acactctacg
5341 cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
5401 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
5461 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
5521 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
5581 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
5641 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
5701 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
5761 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
5821 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
5881 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
5941 cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
6001 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
6061 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
6121 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
6181 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
6241 ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
6301 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
6361 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
6421 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
6481 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
6541 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
6601 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
6661 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
6721 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
6781 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
6841 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
6901 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
6961 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
7021 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
7081 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
7141 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
7201 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
7261 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
7321 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
7381 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
7441 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
7501 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
7561 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
7621 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
7681 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
7741 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
7801 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
7861 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
7921 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
7981 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
8041 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
8101 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
8161 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
8221 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
8281 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
8341 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
8401 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
8461 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
8521 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
8581 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
8641 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
8701 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
8761 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
8821 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
8881 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
8941 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
9001 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
9061 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
9121 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
9181 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
9241 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
9301 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
9361 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
9421 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
9481 agctggagct gcaagc
//
