pDONR207

价格:3500元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Invitrogen
载体名称: pDONR207
质粒类型: Gateway系统的DONR载体
高拷贝/低拷贝: 低拷贝
克隆方法: Gateway
载体大小: 5585
5' 测序引物及序列: pDONR-F: 5'-TCGCGTTAACGCTAGCATGGATCTC-3'
3' 测序引物及序列: pDONR-R: 5'-GTAACATCAGAGATTTTGAGACAC-3'
载体标签: --
载体抗性: 庆大霉素、 氯霉素
备注:
载体本身含有CCDb致死基因,能够使得普通的DH5alpha等细菌死亡,必须 使用DB3.1感受态细胞进行质粒扩增,用来构建Gateway系统入门载体

pDONR207尽管含有pUC质粒的起始复制点,但是细菌内仍然是低产量质粒;

构建Gateway系统入门克隆时需要使用ccdB敏感的 OmniMAX2 感受态细胞。
 
产品目录号: --
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pDONR207载体图谱



pDONR207多克隆位点

pDONR207载体特征

载体简介

载体序列

LOCUS       pDONR207	5585 bp 	DNA   circular	SYN
DEFINITION  pDONR207
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="pDONR207"
                     /mol_type="other DNA"
     terminator      complement(73..100)
                     /label="rrnB_T2_terminator"
     terminator      complement(232..275)
                     /label="rrnB_T1_terminator"
     misc_feature    complement(331..426)
                     /label="attL1"
     misc_feature    332..563
                     /label="attP"
     misc_feature    430..530
                     /label="attR1"
     misc_feature    complement(430..530)
                     /label="attR2"
     misc_feature    complement(959..1264)
                     /label="ccdB"
     gene            complement(1606..2265)
                     /label="CAT/CamR"
                     /gene="CAT/CamR"
     CDS             complement(1606..2265)
                     /label="ORF frame 3"
     misc_feature    complement(2513..2744)
                     /label="attP"
     misc_feature    2546..2646
                     /label="attR2"
     misc_feature    complement(2546..2653)
                     /label="attR1"
     misc_feature    2670..2745
                     /label="attL2"
     CDS             2868..3998
                     /label="ORF frame 3"
     gene            complement(3528..4061)
                     /label="gent"
                     /gene="gent"
     CDS             complement(3528..4061)
                     /label="ORF frame 1"
     CDS             4163..4792
                     /label="ORF frame 2"
     rep_origin      4919..5538
                     /label="pBR322_origin"
ORIGIN
    1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCTAGCC AGGAAGAGTT
   61 TGTAGAAACG CAAAAAGGCC ATCCGTCAGG ATGGCCTTCT GCTTAGTTTG ATGCCTGGCA
  121 GTTTATGGCG GGCGTCCTGC CCGCCACCCT CCGGGCCGTT GCTTCACAAC GTTCAAATCC
  181 GCTCCCGGCG GATTTGTCCT ACTCAGGAGA GCGTTCACCG ACAAACAACA GATAAAACGA
  241 AAGGCCCAGT CTTCCGACTG AGCCTTTCGT TTTATTTGAT GCCTGGCAGT TCCCTACTCT
  301 CGCGTTAACG CTAGCATGGA TCTCGGGCCC CAAATAATGA TTTTATTTTG ACTGATAGTG
  361 ACCTGTTCGT TGCAACAAAT TGATGAGCAA TGCTTTTTTA TAATGCCAAG TTTGTACAAA
  421 AAAGCAGAAC GAGAAACGTA AAATGATATA AATATCAATA TATTAAATTA GATTTTGCAT
  481 AAAAAACAGA CTACATAATA CTGTAAAACA CAACATATCC AGTCACTATG AATCAACTAC
  541 TTAGATGGTA TTAGTGACCT GTAGTCGACC GACAGCCTTC CAAATGTTCT TCGGGTGATG
  601 CTGCCAACTT AGTCGACCGA CAGCCTTCCA AATGTTCTTC TCAAACGGAA TCGTCGTATC
  661 CAGCCTACTC GCTATTGTCC TCAATGCCGT ATTAAATCAT AAAAAGAAAT AAGAAAAAGA
  721 GGTGCGAGCC TCTTTTTTGT GTGACAAAAT AAAAACATCT ACCTATTCAT ATACGCTAGT
  781 GTCATAGTCC TGAAAATCAT CTGCATCAAG AACAATTTCA CAACTCTTAT ACTTTTCTCT
  841 TACAAGTCGT TCGGCTTCAT CTGGATTTTC AGCCTCTATA CTTACTAAAC GTGATAAAGT
  901 TTCTGTAATT TCTACTGTAT CGACCTGCAG ACTGGCTGTG TATAAGGGAG CCTGACATTT
  961 ATATTCCCCA GAACATCAGG TTAATGGCGT TTTTGATGTC ATTTTCGCGG TGGCTGAGAT
 1021 CAGCCACTTC TTCCCCGATA ACGGAGACCG GCACACTGGC CATATCGGTG GTCATCATGC
 1081 GCCAGCTTTC ATCCCCGATA TGCACCACCG GGTAAAGTTC ACGGGAGACT TTATCTGACA
 1141 GCAGACGTGC ACTGGCCAGG GGGATCACCA TCCGTCGCCC GGGCGTGTCA ATAATATCAC
 1201 TCTGTACATC CACAAACAGA CGATAACGGC TCTCTCTTTT ATAGGTGTAA ACCTTAAACT
 1261 GCATTTCACC AGTCCCTGTT CTCGTCAGCA AAAGAGCCGT TCATTTCAAT AAACCGGGCG
 1321 ACCTCAGCCA TCCCTTCCTG ATTTTCCGCT TTCCAGCGTT CGGCACGCAG ACGACGGGCT
 1381 TCATTCTGCA TGGTTGTGCT TACCAGACCG GAGATATTGA CATCATATAT GCCTTGAGCA
 1441 ACTGATAGCT GTCGCTGTCA ACTGTCACTG TAATACGCTG CTTCATAGCA CACCTCTTTT
 1501 TGACATACTT CGGGTATACA TATCAGTATA TATTCTTATA CCGCAAAAAT CAGCGCGCAA
 1561 ATACGCATAC TGTTATCTGG CTTTTAGTAA GCCGGATCCA CGCGATTACG CCCCGCCCTG
 1621 CCACTCATCG CAGTACTGTT GTAATTCATT AAGCATTCTG CCGACATGGA AGCCATCACA
 1681 GACGGCATGA TGAACCTGAA TCGCCAGCGG CATCAGCACC TTGTCGCCTT GCGTATAATA
 1741 TTTGCCCATG GTGAAAACGG GGGCGAAGAA GTTGTCCATA TTGGCCACGT TTAAATCAAA
 1801 ACTGGTGAAA CTCACCCAGG GATTGGCTGA GACGAAAAAC ATATTCTCAA TAAACCCTTT
 1861 AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT TGCGAATATA TGTGTAGAAA
 1921 CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA AACGTTTCAG TTTGCTCATG
 1981 GAAAACGGTG TAACAAGGGT GAACACTATC CCATATCACC AGCTCACCGT CTTTCATTGC
 2041 CATACGGAAT TCCGGATGAG CATTCATCAG GCGGGCAAGA ATGTGAATAA AGGCCGGATA
 2101 AAACTTGTGC TTATTTTTCT TTACGGTCTT TAAAAAGGCC GTAATATCCA GCTGAACGGT
 2161 CTGGTTATAG GTACATTGAG CAACTGACTG AAATGCCTCA AAATGTTCTT TACGATGCCA
 2221 TTGGGATATA TCAACGGTGG TATATCCAGT GATTTTTTTC TCCATTTTAG CTTCCTTAGC
 2281 TCCTGAAAAT CTCGATAACT CAAAAAATAC GCCCGGTAGT GATCTTATTT CATTATGGTG
 2341 AAAGTTGGAA CCTCTTACGT GCCGATCAAC GTCTCATTTT CGCCAAAAGT TGGCCCAGGG
 2401 CTTCCCGGTA TCAACAGGGA CACCAGGATT TATTTATTCT GCGAAGTGAT CTTCCGTCAC
 2461 AGGTATTTAT TCGGCGCAAA GTGCGTCGGG TGATGCTGCC AACTTAGTCG ACTACAGGTC
 2521 ACTAATACCA TCTAAGTAGT TGATTCATAG TGACTGGATA TGTTGTGTTT TACAGTATTA
 2581 TGTAGTCTGT TTTTTATGCA AAATCTAATT TAATATATTG ATATTTATAT CATTTTACGT
 2641 TTCTCGTTCA GCTTTCTTGT ACAAAGTGGG CATTATAAGA AAGCATTGCT TATCAATTTG
 2701 TTGCAACGAA CAGGTCACTA TCAGTCAAAA TAAAATCATT ATTTGCCATC CAGCTGCAGC
 2761 TCTGGCCCGT GTCTCAAAAT CTCTGATGTT ACATTGCACA AGATAAAAAT ATATCATCAT
 2821 GAACAATAAA ACTGTCTGCT TACATAAACA GTAATACAAG GGGTGTTATG AGCCATATTC
 2881 AACGGGAAAC GTCGAGGCCG CGATTAAATT CCAACATGGA TGCTGATTTA TATGGGTATA
 2941 AATGGGCTCG CGATAATGTC GGGCAATCAG GTGCGACAAT CTATCGCTTG TATGGGAAGC
 3001 CCGATGCGCC AGAGTTGTTT CTGAAACATG GCAAAGGTAG CGTTGCCAAT GATGTTACAG
 3061 ATGAGATGGT CAGACTAAAC TGGCTGACGG AATTTATGCC TCTTCCGACC ATCAAGCATT
 3121 TTATCCGTAC TCCTGATGAT GCATGGTTAC TCACCACTGC GATCCCCGGA AAAACAGCAT
 3181 TCCAGGTATT AGAAGAATAT CCTGATTCAG GTGAAAATAT TGTTGATGCG CTGGCAGTGT
 3241 TCCTGCGCCG GTTGCATTCG ATTCCTGTTT GTAATTGTCC TTTTAACAGC GATCGCGTAT
 3301 TTCGTCTCGC TCAGGCGCAA TCACGAATGA ATAACGGTTT GGTTGATGCG AGTGATTTTG
 3361 ATGACGAGCG TAATGGCTGG CCTGTTGAAC AAGTCTGGAA AGAAATGCAT AAACTTTTGC
 3421 CATTCTCACC GGATTCAGTC GTCACTCATG GTGATTTCTC ACTTGATAAC CTTATTTTTG
 3481 ACGAGGGGAA ATTAGGCCGG GAAGCCGATC TCGGCTTGAA CGAATTGTTA GGTGGCGGTA
 3541 CTTGGGTCGA TATCAAAGTG CATCACTTCT TCCCGTATGC CCAACTTTGT ATAGAGAGCC
 3601 ACTGCGGGAT CGTCACCGTA ATCTGCTTGC ACGTAGATCA CATAAGCACC AAGCGCGTTG
 3661 GCCTCATGCT TGAGGAGATT GATGAGCGCG GTGGCAATGC CCTGCCTCCG GTGCTCGCCG
 3721 GAGACTGCGA GATCATAGAT ATAGATCTCA CTACGCGGCT GCTCAAACCT GGGCAGAACG
 3781 TAAGCCGCGA GAGCGCCAAC AACCGCTTCT TGGTCGAAGG CAGCAAGCGC GATGAATGTC
 3841 TTACTACGGA GCAAGTTCCC GAGGTAATCG GAGTCCGGCT GATGTTGGGA GTAGGTGGCT
 3901 ACGTCTCCGA ACTCACGACC GAAAAGATCA AGAGCAGCCC GCATGGATTT GACTTGGTCA
 3961 GGGCCGAGCC TACATGTGCG AATGATGCCC ATACTTGAGC CACCTAACTT TGTTTTAGGG
 4021 CGACTGCCCT GCTGCGTAAC ATCGTTGCTG CTGCGTAACA TCGTTGCTGC TCCATAACAT
 4081 CAAACATCGA CCCACGGCGT AACGCGCTTG CTGCTTGGAT GCCCGAGGCA TAGACTGTAC
 4141 AAAAAAACAG TCATAACAAG CCATGAAAAC CGCCACTGCG CCGTTACCAC CGCTGCGTTC
 4201 GGTCAAGGTT CTGGACCAGT TGCGTGAGCG CATACGCTAC TTGCATTACA GTTTACGAAC
 4261 CGAACAGGCT TATGTCAACT GGGTTCGTGC CTTCATCCGT TTCCACGGTG TGCGTCACCC
 4321 GGCAACCTTG GGCAGCAGCG AAGTCGAGGC ATTTCTGTCC TGGCTGGCGA ACGAGCGCAA
 4381 GGTTTCGGTC TCCACGCATC GTCAGGCATT GGCGGCCTTG CTGTTCTTCT ACGGCAAGGT
 4441 GCTGTGCACG GATCTGCCCT GGCTTCAGGA GATCGGAAGA CCTCGGCCGT CGCGGCGCTT
 4501 GCCGGTGGTG CTGACCCCGG ATGAAGTGGT TCGCATCCTC GGTTTTCTGG AAGGCGAGCA
 4561 TCGTTTGTTC GCCCAGGACT CTAGCTATAG TTCTAGTGGT TGGCTACTAA TAGGTTGTAT
 4621 TGATGTTGGA CGAGTCGGAA TCGCAGACCG ATACCAGGAT CTTGCCATCC TATGGAACTG
 4681 CCTCGGTGAG TTTTCTCCTT CATTACAGAA ACGGCTTTTT CAAAAATATG GTATTGATAA
 4741 TCCTGATATG AATAAATTGC AGTTTCATTT GATGCTCGAT GAGTTTTTCT AATCAGAATT
 4801 GGTTAATTGG TTGTAACACT GGCAGAGCAT TACGCTGACT TGACGGGACG GCGCAAGCTC
 4861 ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG
 4921 ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA
 4981 AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG
 5041 AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG
 5101 TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG
 5161 TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA
 5221 TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC
 5281 TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC
 5341 ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA
 5401 GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT
 5461 CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG
 5521 AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC
 5581 ATGTT
//

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