| 质粒名称: | pIVTRup |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 101362 |
| 存储实验室: | Ángel Raya |
|
载体骨架基本信息 |
|
| 载体名称: | pCRII topo-TA |
| 载体出品公司: | Invitrogene |
| 空载体大小: | 3900bp |
| 完整质粒大小: | 4244bp |
| 载体类型: | For cloning a gene for in vitro transcription purposes |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | 5'UTR-EcoRV site-3'UTR-pAtrack(55 pA) |
| 物种来源: | X. laevis (frog) |
| 启动子: | T7 |
| 克隆方法: | Ligation Independent Cloning |
| 5’测序引物 | M13F |
| 3’测序引物 | M13R |
|
文献引用方法 |
|
| 材料和方法部分: | pIVTRup was a gift from Ángel Raya (Addgene plasmid # 101362 ; http://n2t.net/addgene:101362 ; RRID:Addgene_101362) |
买家导航
pIVTRup载体质粒基本信息
pIVTRup质粒图谱载体图谱和pIVTRup载体序列质粒序列多克隆位点信息
pIVTRup质粒载体简介
pIVTRup质粒序列载体序列
LOCUS pIVTRup 4202 bp DNA circular SYN 15-DEC-2025
DEFINITION Highly efficient in vitro transcription. Empty backbone..
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4202)
TITLE pIVTRup
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 4202)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec 15, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT Sequence Label: pIVTRup
FEATURES Location/Qualifiers
source 1..4202
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(277..293)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(277..293)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(290..312)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 301..317
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(325..331,332..349,350..355))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 370..391
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(508..525)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(679..1267)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(759..778)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(1438..2229,2230..2298))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 2061..2080
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
CDS complement(2316..3110)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2427..2446)
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind 3037..3056
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
rep_origin complement(3454..3882)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3564..3585)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 3776..3795
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4008..4030
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 4022..4039
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 4023..4039
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 4046..4064
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter 4136..4154
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 atcgctgcct tctgcggggc ttgccttctg gccatgccct tcttctctcc cttgcacctg
61 tacctcttgg tctttgaata aagcctgagt aggaagtgag ggtctagaaa aaaaaaaaaa
121 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aacgatcaat aaccgacact
181 cctgcgtcgc ctacgcgcta cgatctgcag aattccagca cactggcggc cgttactagt
241 ggatccgagc tcggtaccaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
301 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
361 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
421 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
481 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
541 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
601 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
661 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
721 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
781 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
841 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
901 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
961 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
1021 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
1081 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
1141 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
1201 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
1261 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
1321 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
1381 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
1441 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
1501 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
1561 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
1621 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
1681 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
1741 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
1801 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
1861 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
1921 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
1981 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
2041 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
2101 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
2161 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
2221 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
2281 gaaatgttga atactcatac tcttcctttt tcaattcaga agaactcgtc aagaaggcga
2341 tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca
2401 gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag
2461 cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc
2521 atgatattcg gcaagcaggc atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg
2581 cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga
2641 tcatcctgat cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc
2701 gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca
2761 gccatgatgg atactttctc ggcaggagca aggtgggatg acaggagatc ctgccccggc
2821 acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg
2881 caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg cagttcattc
2941 agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg
3001 aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc
3061 tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat gcgaaacgat
3121 cctcatcctg tctcttgatc agatcttgat cccctgcgcc atcagatcct tggcggcaag
3181 aaagccatcc agtttacttt gcagggcttc ccaaccttac cagagggcgc cccagctggc
3241 aattccggtt cgcttgctgt ccataaaacc gcccagtcta gctatcgcca tgtaagccca
3301 ctgcaagcta cctgctttct ctttgcgctt gcgttttccc ttgtccagat agcccagtag
3361 ctgacattca tccggggtca gcaccgtttc tgcggactgg ctttctacgt gttccgcttc
3421 ctttagcagc ccttgcgccc tgaattttgt taaaattcgc gttaaatttt tgttaaatca
3481 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
3541 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
3601 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
3661 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
3721 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
3781 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
3841 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct
3901 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
3961 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
4021 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tgggccctct
4081 agatgcatgc tcgagcggcc gccagtgtga tggatttgga ccctcgtaca gaagctaata
4141 cgactcacta tagggaaata agagagaaaa gaagagtaag aagaaatata agagccaccg
4201 at
//
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pIVTRup质粒载体应用举例
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