| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL2A1-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001658 |
| 产品用途: | 3' AAVLINK plasmid for COL2A1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | COL2A1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL2A1-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL2A1-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL2A1-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL2A1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL2A1-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL2A1-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL2A1-FLAG 6200 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6200)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 1, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6200
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..2913
/codon_start=1
/label=COL2A1
/translation="VNLDEREAPVLMAPLAEMALLESRVIVVRLVLWELLEPLGPLAPL
APLVQLASKETEEKLVHKAPWDPQDQLEPGESRVLKAPEVTKERLESLAREA*RDTVAS
LVCRVCPALLVLLETKVLLVLLVLLALEVLLAPSVPLAKMVLMESLAPLGLLVPVDDQA
KPALLVLLEILDPLVLQVPLALASTCPPLLA*ARERRAPTPCSTCGPTRQPVA*DSMTP
RWMPHSSPSTTRLRASAAPRAPARTLLAPAET*NSATLSGRVETTGLTPTKAAPWTP*R
FSATWRLARLASTPIQQTFPRRTGGAARARRRNTSGLEKPSMVASISAMEMTIWLPTLP
TSR*PSYACCPRKAPRTSPTTARTALPIWTKQLATSRRPCSSRAPMTWRSGQRAIAGSR
TLP*RMAARNIPVSGARLLSSTGHRRPHASPSLTLHPWT*EGPSRNSVWT*GRSAS"
misc_feature 1882..1887
/label=SDS
misc_feature 1945..1950
/label=SDS
misc_feature 2008..2013
/label=SDS
misc_feature 2053..2058
/label=SDS
misc_feature 2862..2867
/label=SDS
CDS 2920..2985
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 2989..3420
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3133..3138
/label=SDS
repeat_region 3463..3603
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3463..3592
/label=AAV2 ITR
rep_origin 3678..4133
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4415..4519
/gene="bla"
/label=AmpR promoter
CDS 4520..5380
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5551..6139
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtga acctggacga
1561 gagggaagcc ccggtgctga tggcccccct ggcagagatg gcgctgctgg agtcaagggt
1621 gatcgtggtg agactggtgc tgtgggagct cctggagccc ctgggccccc tggctcccct
1681 ggccccgctg gtccaactgg caagcaagga gacagaggag aagctggtgc acaaggcccc
1741 atgggaccct caggaccagc tggagcccgg ggaatccagg gtcctcaagg ccccagaggt
1801 gacaaaggag aggctggaga gcctggcgag agaggcctga agggacaccg tggcttcact
1861 ggtctgcagg gtctgcccgg ccctcctggt ccttctggag accaaggtgc ttctggtcct
1921 gctggtcctt ctggccctag aggtcctcct ggccccgtcg gtccctctgg caaagatggt
1981 gctaatggaa tccctggccc cattgggcct cctggtcccc gtggacgatc aggcgaaacc
2041 ggccctgctg gtcctcctgg aaatcctgga ccccctggtc ctccaggtcc ccctggccct
2101 ggcatcgaca tgtccgcctt tgctggctta ggcccgagag agaagggccc cgaccccctg
2161 cagtacatgc gggccgacca ggcagccggt ggcctgagac agcatgacgc cgaggtggat
2221 gccacactca agtccctcaa caaccagatt gagagcatcc gcagccccga gggctcccgc
2281 aagaaccctg ctcgcacctg cagagacctg aaactctgcc accctgagtg gaagagtgga
2341 gactactgga ttgaccccaa ccaaggctgc accttggacg ccatgaaggt tttctgcaac
2401 atggagactg gcgagacttg cgtctacccc aatccagcaa acgttcccaa gaagaactgg
2461 tggagcagca agagcaagga gaagaaacac atctggtttg gagaaaccat caatggtggc
2521 ttccatttca gctatggaga tgacaatctg gctcccaaca ctgccaacgt ccagatgacc
2581 ttcctacgcc tgctgtccac ggaaggctcc cagaacatca cctaccactg caagaacagc
2641 attgcctatc tggacgaagc agctggcaac ctcaagaagg ccctgctcat ccagggctcc
2701 aatgacgtgg agatccgggc agagggcaat agcaggttca cgtacactgc cctgaaggat
2761 ggctgcacga aacataccgg taagtggggc aagactgtta tcgagtaccg gtcacagaag
2821 acctcacgcc tccccatcat tgacattgca cccatggaca taggagggcc cgagcaggaa
2881 ttcggtgtgg acatagggcc ggtctgcttc ttgtgtacag actacaaaga ccatgacggt
2941 gattataaag atcatgacat cgactacaag gatgacgatg acaagtaact cgagataatc
3001 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
3061 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
3121 ctttcatttt ctcctccttg tataaatcct ggttagttct tgccacggcg gaactcatcg
3181 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
3241 tgtttatttg tgaaatttgt gatgctattg ctttatttgt aaccatctag ctttatttgt
3301 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
3361 aacaacaatt gcattcattt tatgtttcag gttcaggggg agatgtggga ggttttttaa
3421 agatctggag accggtgtgg cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg
3481 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
3541 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc
3601 aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
3661 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
3721 tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt tcgctttctt
3781 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
3841 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
3901 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
3961 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact ctatctcggg
4021 ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa aaaatgagct
4081 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
4141 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
4201 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
4261 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
4321 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
4381 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
4441 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
4501 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
4561 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
4621 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
4681 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
4741 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
4801 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
4861 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
4921 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
4981 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
5041 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
5101 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
5161 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
5221 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
5281 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
5341 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
5401 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
5461 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
5521 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
5581 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
5641 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
5701 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
5761 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
5821 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
5881 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
5941 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
6001 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
6061 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
6121 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
6181 ctggcctttt gctcacatgt
//
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL2A1-FLAG载体图谱质粒图谱pdf版和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-COL2A1-FLAG载体序列质粒序列等相关资料下载
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