LOCUS pCD/NL-BH*DDD 9514 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pCD/NL-BH*DDD
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9514)
AUTHORS Zhang XY, La Russa VF, Reiser J
TITLE Transduction of bone-marrow-derived mesenchymal stem cells by using
lentivirus vectors pseudotyped with modified RD114 envelope
glycoproteins.
JOURNAL J Virol. 2004 Feb . 78(3):1219-29.
PUBMED 14722277
REFERENCE 2 (bases 1 to 9514)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9514
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(33..50)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(204..792)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(284..303)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(963..1823)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1586..1605
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1824..1928)
/gene="bla"
/label=AmpR promoter
primer_bind complement(2003..2022)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 2194..2573
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2574..2777
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2728..2748
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 2774..2798
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 2877..4379
/codon_start=1
/gene="gag"
/product="gag protein from human immunodeficiency virus 1"
/label=HIV-1 gag
/translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGP
IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
CDS 4172..7183
/codon_start=1
/gene="pol"
/product="pol protein from human immunodeficiency virus 1"
/label=HIV-1 pol
/translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
MAGDDCVASRQDED"
misc_feature 6868..6985
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
rep_origin 7765..7900
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 7827..7846
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
misc_feature 8068..8301
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 8486..8530
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
CDS 8824..8850
/codon_start=1
/product="nuclear export signal from the HIV Rev protein
(Fischer et al., 1995)"
/label=NES
/translation="LPPLERLTL"
primer_bind complement(9229..9246)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 9235..9459
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
ORIGIN
1 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
61 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
121 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
181 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
241 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
301 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
361 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
421 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
481 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
541 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
601 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
661 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
721 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
781 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
841 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
901 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
961 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
1021 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
1081 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
1141 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
1201 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
1261 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
1321 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
1381 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
1441 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
1501 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
1561 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
1621 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
1681 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
1741 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
1801 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
1861 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
1921 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg
1981 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
2041 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
2101 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
2161 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
2221 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
2281 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
2341 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
2401 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
2461 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
2521 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
2581 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
2641 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
2701 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
2761 tctatataag cagagctcgt ttagtgaacc gtcagatctc tagaagctct ctcgacgcag
2821 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagagatgg
2881 gtgcgagagc gtcggtatta agcgggggag aattagataa atgggaaaaa attcggttaa
2941 ggccaggggg aaagaaacaa tataaactaa aacatatagt atgggcaagc agggagctag
3001 aacgattcgc agttaatcct ggccttttag agacatcaga aggctgtaga caaatactgg
3061 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacaa
3121 tagcagtcct ctattgtgtg catcaaagga tagatgtaaa agacaccaag gaagccttag
3181 ataagataga ggaagagcaa aacaaaagta agaaaaaggc acagcaagca gcagctgaca
3241 caggaaacaa cagccaggtc agccaaaatt accctatagt gcagaacctc caggggcaaa
3301 tggtacatca ggccatatca cctagaactt taaatgcatg ggtaaaagta gtagaagaga
3361 aggctttcag cccagaagta atacccatgt tttcagcatt atcagaagga gccaccccac
3421 aagatttaaa taccatgcta aacacagtgg ggggacatca agcagccatg caaatgttaa
3481 aagagaccat caatgaggaa gctgcagaat gggatagatt gcatccagtg catgcagggc
3541 ctattgcacc aggccagatg agagaaccaa ggggaagtga catagcagga actactagta
3601 cccttcagga acaaatagga tggatgacac ataatccacc tatcccagta ggagaaatct
3661 ataaaagatg gataatcctg ggattaaata aaatagtaag aatgtatagc cctaccagca
3721 ttctggacat aagacaagga ccaaaggaac cctttagaga ctatgtagac cgattctata
3781 aaactctaag agccgagcaa gcttcacaag aggtaaaaaa ttggatgaca gaaaccttgt
3841 tggtccaaaa tgcgaaccca gattgtaaga ctattttaaa agcattggga ccaggagcga
3901 cactagaaga aatgatgaca gcatgtcagg gagtgggggg acccggccat aaagcaagag
3961 ttttggctga agcaatgagc caagtaacaa atccagctac cataatgata cagaaaggca
4021 attttaggaa ccaaagaaag actgttaagt gtttcaattg tggcaaagaa gggcacatag
4081 ccaaaaattg cagggcccct aggaaaaagg gctgttggaa atgtggaaag gaaggacacc
4141 aaatgaaaga ttgtactgag agacaggcta attttttagg gaagatctgg ccttcccaca
4201 agggaaggcc agggaatttt cttcagagca gaccagagcc aacagcccca ccagaagaga
4261 gcttcaggtt tggggaagag acaacaactc cctctcagaa gcaggagccg atagacaagg
4321 aactgtatcc tttagcttcc ctcagatcac tctttggcag cgacccctcg tcacaataaa
4381 gatagggggg caattaaagg aagctctatt agatacagga gcagatgata cagtattaga
4441 agaaatgaat ttgccaggaa gatggaaacc aaaaatgata gggggaattg gaggttttat
4501 caaagtaaga cagtatgatc agatactcat agaaatctgc ggacataaag ctataggtac
4561 agtattagta ggacctacac ctgtcaacat aattggaaga aatctgttga ctcagattgg
4621 ctgcacttta aattttccca ttagtcctat tgagactgta ccagtaaaat taaagccagg
4681 aatggatggc ccaaaagtta aacaatggcc attgacagaa gaaaaaataa aagcattagt
4741 agaaatttgt acagaaatgg aaaaggaagg aaaaatttca aaaattgggc ctgaaaatcc
4801 atacaatact ccagtatttg ccataaagaa aaaagacagt actaaatgga gaaaattagt
4861 agatttcaga gaacttaata agagaactca agatttctgg gaagttcaat taggaatacc
4921 acatcctgca gggttaaaac agaaaaaatc agtaacagta ctggatgtgg gcgatgcata
4981 tttttcagtt cccttagata aagacttcag gaagtatact gcatttacca tacctagtat
5041 aaacaatgag acaccaggga ttagatatca gtacaatgtg cttccacagg gatggaaagg
5101 atcaccagca atattccagt gtagcatgac aaaaatctta gagcctttta gaaaacaaaa
5161 tccagacata gtcatctatc aatacatgga tgatttgtat gtaggatctg acttagaaat
5221 agggcagcat agaacaaaaa tagaggaact gagacaacat ctgttgaggt ggggatttac
5281 cacaccagac aaaaaacatc agaaagaacc tccattcctt tggatgggtt atgaactcca
5341 tcctgataaa tggacagtac agcctatagt gctgccagaa aaggacagct ggactgtcaa
5401 tgacatacag aaattagtgg gaaaattgaa ttgggcaagt cagatttatg cagggattaa
5461 agtaaggcaa ttatgtaaac ttcttagggg aaccaaagca ctaacagaag tagtaccact
5521 aacagaagaa gcagagctag aactggcaga aaacagggag attctaaaag aaccggtaca
5581 tggagtgtat tatgacccat caaaagactt aatagcagaa atacagaagc aggggcaagg
5641 ccaatggaca tatcaaattt atcaagagcc atttaaaaat ctgaaaacag gaaagtatgc
5701 aagaatgaag ggtgcccaca ctaatgatgt gaaacaatta acagaggcag tacaaaaaat
5761 agccacagaa agcatagtaa tatggggaaa gactcctaaa tttaaattac ccatacaaaa
5821 ggaaacatgg gaagcatggt ggacagagta ttggcaagcc acctggattc ctgagtggga
5881 gtttgtcaat acccctccct tagtgaagtt atggtaccag ttagagaaag aacccataat
5941 aggagcagaa actttctatg tagatggggc agccaatagg gaaactaaat taggaaaagc
6001 aggatatgta actgacagag gaagacaaaa agttgtcccc ctaacggaca caacaaatca
6061 gaagactgag ttacaagcaa ttcatctagc tttgcaggat tcgggattag aagtaaacat
6121 agtgacagac tcacaatatg cattgggaat cattcaagca caaccagata agagtgaatc
6181 agagttagtc agtcaaataa tagagcagtt aataaaaaag gaaaaagtct acctggcatg
6241 ggtaccagca cacaaaggaa ttggaggaaa tgaacaagta gataaattgg tcagtgctgg
6301 aatcaggaaa gtactatttt tagatggaat agataaggcc caagaagaac atgagaaata
6361 tcacagtaat tggagagcaa tggctagtga ttttaaccta ccacctgtag tagcaaaaga
6421 aatagtagcc agctgtgata aatgtcagct aaaaggggaa gccatgcatg gacaagtaga
6481 ctgtagccca ggaatatggc agctagattg tacacattta gaaggaaaag ttatcttggt
6541 agcagttcat gtagccagtg gatatataga agcagaagta attccagcag agacagggca
6601 agaaacagca tacttcctct taaaattagc aggaagatgg ccagtaaaaa cagtacatac
6661 agacaatggc agcaatttca ccagtactac agttaaggcc gcctgttggt gggcggggat
6721 caagcaggaa tttggcattc cctacaatcc ccaaagtcaa ggagtaatag aatctatgaa
6781 taaagaatta aagaaaatta taggacaggt aagagatcag gctgaacatc ttaagacagc
6841 agtacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
6901 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
6961 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg
7021 gaaaggacca gcaaagctcc tctggaaagg tgaaggggca gtagtaatac aagataatag
7081 tgacataaaa gtagtgccaa gaagaaaagc aaagatcatc agggattatg gaaaacagat
7141 ggcaggtgat gattgtgtgg caagtagaca ggatgaggat taacacatgg aaaagattag
7201 taaaataata agaattctgc aacaactgct gtttatccat ttcagaattg ggtgtcgaca
7261 tagcagaata ggcgttactc gacagaggag agcaagaaat ggagccagta gatcctagac
7321 tagagccctg gaagcatcca ggaagtcagc ctaaaactgc ttgtaccaat tgctattgta
7381 aaaagtgttg ctttcattgc caagtttgtt tcatgacaaa agccttaggc atctcctatg
7441 gcaggaagaa gcggagacag cgacgaagag ctcatcagaa cagtcagact catcaagctt
7501 ctctatcaaa gcagtaagta gtacatgtaa tttaattgat agactaatag aaagagcaga
7561 agacagtggc aatgagagtg aaggagaagt atcagcactt gtggagatgg gggtggaaat
7621 ggggcaccat gctccttggg atattgatga tctgtagtgc tacagaaaaa ttgtgggtca
7681 cagtctatta tggggtacct gtgtggaagg aagcaaccac cactctattt tgtgcatcag
7741 atgctaaagc atatgactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
7801 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
7861 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaatgcata
7921 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
7981 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
8041 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
8101 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
8161 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
8221 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
8281 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
8341 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaataacat
8401 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
8461 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
8521 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
8581 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctgtagtgaa
8641 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa tcccgagggg
8701 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
8761 tcgattagtg aacggatcct tggcacttat ctgggacgat ctgcggagcc tgtgcctctt
8821 cagctaccac cgcttgagag acttactctt gattgtaacg aggattgtgg aacttctggg
8881 acgcaggggg tgggaagccc tcaaatattg gtggaatctc ctacagtatt ggagtcagga
8941 actaaagaat agtgctgtta gcttgctcaa tgccacagcc atagcagtag ctgaggggac
9001 agatagggtt atagaagtag tacaaggagc ttgtagagct attcgccaca tacctagaag
9061 aataagacag ggcttggaaa ggattttgct ataagatggg tggcaagtgg tcaaaaagta
9121 gtgtgattgg atggcctact gtaagggaaa gaatgagacg agctgagcca gcagcagata
9181 gggtgggagc agcatctcga gtctagagcg tttaaacccg ctgatcagcc tcgactgtgc
9241 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
9301 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
9361 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
9421 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
9481 gctgcattaa tgaatcggcc aacgcgcggg gaga
//
