| 质粒名称: | pAAVLINK-3'-BAZ2B-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001684 |
| 产品用途: | 3' AAVLINK plasmid for BAZ2B expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | Promoterless |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | BAZ2B |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-3'-BAZ2B-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-3'-BAZ2B-FLAG载体质粒基本信息
pAAVLINK-3'-BAZ2B-FLAG质粒图谱载体图谱和pAAVLINK-3'-BAZ2B-FLAG载体序列质粒序列多克隆位点信息
pAAVLINK-3'-BAZ2B-FLAG质粒载体简介
pAAVLINK-3'-BAZ2B-FLAG质粒序列载体序列
LOCUS pAAVLINK-3'-BAZ2B-FLAG 6815 bp DNA circular SYN 19-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6815)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 2, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6815
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
protein_bind 145..178
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 179..270
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 271..3554
/codon_start=1
/label=BAZ2B
/translation="DLFSLEVHFQTVSWWCSSYETLVKFWALM*ILMFQT*VFFKRDC*
I*GTAWVKYKTCL*GSSQLLYVIQV**QDTRLKQLLENIC*MLV*IETMFPRFYRYLWK
PTVDKLSLLKV*RPKLFRLTLQHRKLQSWLS*SMNWHAARVWSVKSTRTLIICQT*GEI
NGW*KVNSASSESFMLRKQAKETLQVALIWEKSSIPWAHPLQDASEEGREETVIMTMMM
TMTVMTKGMKMMRMKKIKKTKKEKRLISVKMRMKVTKQQVLKSWKNRLKN*VNNRVSTE
GSSLMRLTHCVQ*CLAKIVTDAGTGFFPNVGGFL*KAWRVVKD*KKLQKKEKN*KRQKV
SRSKKKCLRLLGTV*IVQIQITVNKRKILKKKITQIYSFRNLALFPN*ASFWK*LRCLL
SQRL*PPNQMLVQMGARCLIRTVENIHWAAFSQQQRKAMWKRQTLIICLILVQVVQGSS
TVLSPMTSY*KR*LKRIDNGLVFCHEHPVMTLHLLMPICQLLLW*LLSLSHHLSHLHLP
QLLLDLLLRILLA*IHLLYHLFR*KVEYL*WDFSFVDGPLVWLLLIFHLHHLYLV*DRG
*GYQKEMVIHS*LPMLLQVKVNLQYHRMKRPLQLNLQLLK*QNQ*IFLVQNLFQKFYLF
ILT*KCSLVGGELLTQRT*KLCSKCCISEE*EKRHYKNKFRNIWIILLKPASRIRMLLL
LN*MKMKKTK*LEILWRTGQ*KNKQWKWI*VSFNR*KI*KGELHQQVCK*RVGCVQSLH
QKGRTWYILNINHLLNCARSMMENLLAKTKAVHMH*NGRVTTP*I*L*PGWLIWSGTLK
EELKRILLQGSGYGEGHYQKLAVLHR*LCAFSNYRNQ*HGKNQL*KFTAKSVEREIMKN
CFFFVMAVTKAVIPTAIDPRLQQSQMETGFVQLALLRQVVKL*KSKNFMSKEKRLMSQR
KARR*L*QEILKMKTLHLQVVH*KEETKTSRKEKWRKTLLLTCQNKKVLLQLRNLKEMT
PRT*LFAV*F*LKWKLMRMHGLFYFL*T*NLFLVIRKLLRSLWIFPQLERN*VVDSIQT
LKPLL*MSGLFLTTVKHLMKMILI*AELATI*GSILKKSGQILSK"
misc_feature 1461..1466
/label=SDS
CDS 2164..2187
/codon_start=1
/label=BAZ2B
/translation="FYLFILT"
CDS 3561..3626
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3630..4061
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3774..3779
/label=SDS
repeat_region 4078..4218
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4078..4207
/label=AAV2 ITR
rep_origin 4293..4748
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5030..5134
/gene="bla"
/label=AmpR promoter
CDS 5135..5995
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6166..6754
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtataact tcgtatagga tactttatag caatttatga
181 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
241 ctgacatcca ctttgccttt ctctccacag gacttgttct ctctggaagt acattttcag
301 actgtctcat ggtggtgcag ttcttacgaa actttggtaa agttttgggc tttgatgtga
361 atattgatgt tccaaacctg agtgttcttc aagagggatt gctaaatata ggggacagca
421 tgggtgaagt acaagacttg cttgtgaggc tcctctcagc tgctgtatgt gatccaggtc
481 taataacagg atacaaggct aaaacagctc ttggagaaca tttgctgaat gttggtgtga
541 atcgagacaa tgtttccgag attttacaga tatttatgga agcccactgt ggacaaactg
601 agcttactga aagtctgaag accaaagctt ttcaggctca cactccagca cagaaagctt
661 cagtcctggc tttcctgatc aatgaactgg catgcagcaa gagtgtggtc agtgaaatcg
721 acaagaacat tgattatatg tcaaacttga ggagagataa atgggtggta gaaggtaaac
781 tccgcaagct cagaatcatt catgctaaga aaacaggcaa aagagacact tcaggtggca
841 ttgatctggg agaagagcag catcccttgg gcacacccac tccaggacgc aagcgaagaa
901 ggaagggagg agacagtgat tatgacgatg atgatgacga tgacagtgat gaccaagggg
961 atgaagatga tgaggatgaa gaagataaag aagacaaaaa aggaaaaaag actgatatct
1021 gtgaagatga ggatgaaggt gaccaagcag caagtgttga agagctggaa aaacagattg
1081 aaaaactgag taaacaacag agtcagtaca gaaggaagct ctttgatgcg tctcactcat
1141 tgcgttcagt gatgtttggc caagatcgtt acagacgccg gtactggatt cttccccaat
1201 gtggggggat ttttgtagaa ggcatggaga gtggtgaagg actagaagaa attgcaaaag
1261 aaagagaaaa actgaaaaag gcagaaagtg tccagatcaa agaagaaatg tttgagactt
1321 ctggggacag tttaaattgt tcaaatacag atcactgtga acaaaaggaa gatcttaaag
1381 aaaaagataa cacaaatcta ttccttcaga aacctggctc tttttccaaa ttaagcaagc
1441 ttttggaagt agctaagatg cctcctgagt cagaggttat gacccccaaa ccaaatgctg
1501 gtgcaaatgg gtgcacgttg tcttatcaga acagtggaaa acattcactg ggcagcgttc
1561 agtcaacagc aacgcaaagc aatgtggaaa aggcagactc taataatctg tttaatactg
1621 gttcaagtgg tccagggaag ttctacagtc ctctccccaa tgaccagtta ctaaaaacgc
1681 tgactgaaaa gaatagacaa tggtttagtc ttttgccacg aacaccctgt gatgacactt
1741 cacttactca tgccgatatg tcaactgctt ctttggtgac tcctcagtct cagccaccat
1801 ctaagtcacc ttcacctacc ccagctcctc ttggatcttc tgctcagaat cctgttggct
1861 taaatccatt tgctttatca cctcttcagg tgaaaggtgg agtatctatg atgggacttc
1921 agttttgtgg atggcccact ggtgtggtta cttctaatat tccatttaca tcatctgtac
1981 ctagtctagg atcggggtta gggttatcag aaggaaatgg taattcattc ttgacttcca
2041 atgttgcttc aagtaaaagt gaatctccag taccacagaa tgaaaaggcc acttcagctc
2101 aacctgcagc tgttgaagta gcaaaaccag tagattttcc tagtccaaaa cctattccag
2161 aagttctact tatttatctt gacttagaaa tgcagtttgg ttggtggaga attattgacc
2221 cagaggacct aaaagctttg ctcaaagtgc tgcatctcag aggaataaga gaaaaggcat
2281 tacaaaaaca aattcagaaa catttggatt atattactca agcctgcctc aagaataagg
2341 atgttgctat tattgaactg aatgaaaatg aagaaaacca agtaactcga gatattgtgg
2401 agaactggtc agtagaagaa caagcaatgg aaatggattt gagtgtcctt caacaggtag
2461 aagatctaga aaggagagtt gcatcagcaa gtttgcaagt gaagggttgg atgtgtccag
2521 agcctgcatc agaaagggag gacttggtat attttgaaca taaatcattt actaaattgt
2581 gcaaggagca tgatggagaa tttactggcg aagacgaaag cagtgcacat gcactagaac
2641 ggaagagtga caacccccta gatatagctg taaccaggct ggctgatttg gagcggaaca
2701 ttgaaagaag aattgaagag gatattgctc cagggctcag ggtatggaga agggcattat
2761 cagaagctcg cagtgctgca caggtagctc tgtgcattca gcaattacag aaatcaatag
2821 catgggaaaa atcaattatg aaagtttact gccaaatctg tcgaaaggga gataatgaag
2881 aactgcttct tctttgtgat ggctgtgaca aaggctgtca tacctactgc catagaccca
2941 agattacaac aatcccagat ggagactggt tttgtccagc ttgcattgct aaggcaagtg
3001 gtcaaactct aaaaatcaaa aaacttcatg tcaaaggaaa aaagactaat gagtcaaaga
3061 aaggcaagaa ggtaacttta acaggagata ctgaagatga agactctgca tctacaagta
3121 gttcactaaa aagaggaaac aaagacctca agaaaagaaa aatggaggaa aacacttcta
3181 ttaacttgtc aaaacaagaa agttttactt cagttaagaa acctaaaaga gatgactcca
3241 aggacctagc tctttgcagt atgattctga ctgaaatgga aactcatgag gatgcatggc
3301 cttttctact tcctgtaaac ttgaaacttg ttcctggtta taagaaagtt attaagaagc
3361 ctatggattt ttccacaatt agagagaaac taagtagtgg acagtatcca aaccttgaaa
3421 cctttgctct agatgtcagg cttgtttttg acaactgtga aacatttaat gaagatgatt
3481 ctgatatagg cagagctggc cacaatatga ggaagtattt tgaaaaaaag tggacagata
3541 ctttcaaagt gagctgtaca gactacaaag accatgacgg tgattataaa gatcatgaca
3601 tcgactacaa ggatgacgat gacaagtaac tcgagataat caacctctgg attacaaaat
3661 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
3721 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
3781 gtataaatcc tggttagttc ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
3841 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgtttattt gtgaaatttg
3901 tgatgctatt gctttatttg taaccatcta gctttatttg tgaaatttgt gatgctattg
3961 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
4021 ttatgtttca ggttcagggg gagatgtggg aggtttttta acggaccgag cggccgcagg
4081 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
4141 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
4201 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
4261 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
4321 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc
4381 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
4441 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
4501 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
4561 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
4621 caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg
4681 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
4741 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
4801 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
4861 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
4921 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
4981 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
5041 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
5101 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
5161 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
5221 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
5281 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
5341 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
5401 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
5461 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
5521 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
5581 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
5641 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
5701 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
5761 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
5821 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
5881 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
5941 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
6001 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
6061 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
6121 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
6181 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
6241 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
6301 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
6361 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
6421 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
6481 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
6541 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
6601 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
6661 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
6721 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
6781 gttcctggcc ttttgctggc cttttgctca catgt
//
pAAVLINK-3'-BAZ2B-FLAG载体图谱质粒图谱pdf版和pAAVLINK-3'-BAZ2B-FLAG载体序列质粒序列等相关资料下载
pAAVLINK-3'-BAZ2B-FLAG质粒载体应用举例
- 上一篇：pAAVLINK-EF1a-HA-5'-BAZ2B
- 下一篇：没有了
