| 出品公司: | Novagen (EMD Millipore) |
|---|---|
| 载体名称: | pET-9a |
| 质粒类型: | 细菌表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | T7 |
| 载体大小: | 4341 bp |
| 5' 测序引物及序列: | T7-Fwd |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 卡那霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | 与pET3相同，但kanR；a、b、c、d因MCS细菌表达载体的不同而不同，其克隆位点为BamHI。 |
| 产品目录号: | -- |
| 稳定性: | 瞬态表达 |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | 非病毒 |
买家导航
pET9a载体基本信息
pET9a载体图谱质粒图谱和多克隆位点信息
pET9a载体简介
pET9a载体序列
LOCUS pET-9a 4341 bp ds-DNA circular SYN 23-OCT-2018
DEFINITION Bacterial expression vector with a BamHI cloning site. For
alternative reading frames, use pET-9b or pET-9c.
ACCESSION .
VERSION .
KEYWORDS pET-9a
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4341)
AUTHORS MilliporeSigma (Novagen)
TITLE Direct Submission
JOURNAL Exported Saturday, January 11, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4341
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
terminator 404..451
/note="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
CDS complement(519..551)
/codon_start=1
/product="epitope tag from a T7 major capsid protein"
/note="T7 tag"
/translation="MASMTGGQQMG"
RBS 560..565
/note="ribosome binding site"
promoter complement(613..631)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 2192..2383
/codon_start=1
/gene="rop"
/product="Rop protein"
/note="rop"
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
rep_origin complement(2813..3401)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS 3523..4338
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga
421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact
481 cagcttcctt tcgggctttg ttagcagccg gatccgcgac ccatttgctg tccaccagtc
541 atgctagcca tatgtatatc tccttcttaa agttaaacaa aattatttct agagggaaac
601 cgttgtggtc tccctatagt gagtcgtatt aatttcgcgg gatcgagatc tcgatcctct
661 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata
721 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt
781 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc
841 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc
901 taatgcagga gtcgcataag ggagagcgtc gaccgatgcc cttgagagcc ttcaacccag
961 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct
1021 ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc ggcgaggacc
1081 gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg
1141 ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag aagcaggcca
1201 ttatcgccgg catggcggcc gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag
1261 gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg atgcccgcgt
1321 tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt caaggatcgc
1381 tcgcggctct taccagccta acttcgatca ctggaccgct gatcgtcacg gcgatttatg
1441 ccgcctcggc gagcacatgg aacgggttgg catggattgt aggcgccgcc ctataccttg
1501 tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc tgaatggaag
1561 ccggcggcac ctcgctaacg gattcaccac tccaagaatt ggagccaatc aattcttgcg
1621 gagaactgtg aatgcgcaaa ccaacccttg gcagaacata tccatcgcgt ccgccatctc
1681 cagcagccgc acgcggcgca tctcgggcag cgttgggtcc tggccacggg tgcgcatgat
1741 cgtgctcctg tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa
1801 tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg
1861 agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc
1921 agcgccctgc accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg
1981 aacacctaca tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc
2041 ccgccgcatc cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc
2101 atcatcagta acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat
2161 gaacagaaat cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa
2221 catggcccgc tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga
2281 cgcggatgaa caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg
2341 cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
2401 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
2461 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
2521 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatatgc
2581 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt
2641 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
2701 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
2761 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
2821 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
2881 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
2941 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
3001 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
3061 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
3121 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
3181 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
3241 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
3301 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
3361 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
3421 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgaaca
3481 ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg
3541 gaaacgtctt gctcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
3601 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag
3661 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
3721 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
3781 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg gaaaacagca
3841 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
3901 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta
3961 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
4021 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taagcttttg
4081 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
4141 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
4201 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
4261 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
4321 ctcgatgagt ttttctaaga a
//
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