PCDH-METTL14-R298P

LOCUS       PCDH-METTL14-R298P                9590 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Overexpression of METTL14 mutant in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    PCDH-METTL14-R298P
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9590)
  AUTHORS   Weng H, Huang H, Wu H, Qin X, Zhao BS, Dong L, Shi H, Skibbe J, Shen
            C, Hu C, Sheng Y, Wang Y, Wunderlich M, Zhang B, Dore LC, Su R, Deng
            X, Ferchen K, Li C, Sun M, Lu Z, Jiang X, Marcucci G, Mulloy JC, 
            Yang J, Qian Z, Wei M, He C, Chen J
  TITLE     METTL14 Inhibits Hematopoietic Stem/Progenitor Differentiation and 
            Promotes Leukemogenesis via mRNA m(6)A Modification.
  JOURNAL   Cell Stem Cell. 2018 Feb 1;22(2):191-205.e9. doi: 
            10.1016/j.stem.2017.11.016. Epub 2017 Dec 28.
  PUBMED    29290617
REFERENCE   2  (bases 1 to 9590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9590
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    52..168
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        258..461
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     412..432
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     458..482
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             541..567
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        1977..2188
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2201..2469
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      2498..2507
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2528..3190
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             3260..3313
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3314..3913
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3314..3333)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3810..3830
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3914..4502
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3967..3987)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4385..4396)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4576..4809
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4881..5002
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4918..4937)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4972..4991
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5042..5177
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5104..5123
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(5210..5226)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5210..5226)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5223..5245)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5234..5250
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5258..5288)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5303..5324
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5441..5458)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5612..6200)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5692..5711)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6371..7231)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6994..7013
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7232..7336)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7404..7422
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7460..7482)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7582..7601
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7795..7817
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7809..7826
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7810..7826
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7841..8067
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8068..8248
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8295..8420
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8913..9146
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9331..9375
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag
       61 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
      121 catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat cgatactagt
      181 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      241 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      301 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      361 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      421 gcggtaggcg tgtacggtgg gaggtttata taagcagagc tcgtttagtg aaccgtcaga
      481 tcgcctggag acgccatcca cgctgttttg acctccatag aagattctag aaccaccatg
      541 tacccatacg atgttccaga ttacgctgat agccgcttgc aggagatccg ggagcggcag
      601 aagttacggc gacagctcct cgcgcagcag ttgggagctg aaagtgccga cagcattggt
      661 gccgtgttaa atagcaaaga tgagcagaga gaaattgctg aaacaagaga aacttgcagg
      721 gcttcctatg atacctctgc tccaaatgca aaacgtaagt atctggatga aggagagaca
      781 gatgaagaca aaatggaaga atataaggat gaactagaaa tgcaacagga tgaagaaaat
      841 ttgccatatg aagaagagat ttacaaagat tctagtactt ttcttaaggg aacacagagc
      901 ttaaatcccc ataatgatta ctgccaacat tttgtagaca ctggacatag acctcagaat
      961 ttcatcaggg atgtaggttt agctgacaga tttgaagaat atcctaaact gagggagctc
     1021 atcaggctaa aggatgagtt aatagctaaa tctaacactc ctcccatgta cttacaagcc
     1081 gatatagaag cctttgacat cagagaacta acacccaaat ttgatgtgat tcttctggaa
     1141 ccccctttag aagaatatta cagagaaact ggcatcactg ctaatgaaaa atgctggact
     1201 tgggatgata ttatgaagtt agaaattgat gagattgcag cacctcgatc atttattttt
     1261 ctctggtgtg gttctgggga ggggttggac cttggaagag tgtgtttacg aaaatggggt
     1321 tacagaagat gtgaagatat ttgttggatt aaaaccaata aaaacaatcc tgggaagact
     1381 aagactttag atccaaaggc tgtctttcag agaacaaagg aacactgcct catggggatc
     1441 aaaggaactg tgaagcctag cacagacggg gacttcattc atgctaatgt tgacattgac
     1501 ttaattatca cagaagaacc tgaaattggc aatatagaaa aacctgtaga aatttttcat
     1561 ataattgagc atttttgtct tggtagaaga cgccttcatc tatttggaag agatagtaca
     1621 attcgaccag gctggctcac agttggacca acgcttacaa atagcaacta caatgcagaa
     1681 acatatgcat cctatttcag tgctcctaat tcctacttga ctggttgtac agaagaaatt
     1741 gagagacttc gaccaaaatc gcctcctccc aaatctaaat ctgaccgagg aggtggagct
     1801 cccagaggtg gaggaagagg tggaacttct gctggccgtg gacgagaaag aaatagatct
     1861 aacttccgag gagaaagagg tggctttaga gggggccgtg gaggagcaca cagaggtggc
     1921 tttccacctc gataagcggc cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc
     1981 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg
     2041 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     2101 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     2161 tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc
     2221 acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc
     2281 tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc
     2341 gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac
     2401 gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg
     2461 ttacagatcc aagctgtgac cggcgcctac gctagacgcc accatggaga gcgacgagag
     2521 cggcctgccc gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt
     2581 cgagctggtg ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa
     2641 gagcaccaaa ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg
     2701 cttctaccac ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa
     2761 caacggcggc tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt
     2821 gagcttcagc taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac
     2881 cggcttcccc gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt
     2941 ggagcacctg caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag
     3001 cctgcgcgac ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc
     3061 catccacccc agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga
     3121 gctgcacagc aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat
     3181 cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc
     3241 cggctccacc ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga
     3301 gaatcccggc cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     3361 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     3421 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     3481 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     3541 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     3601 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     3661 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     3721 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     3781 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     3841 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     3901 gcccggtgcc tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     3961 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     4021 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     4081 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     4141 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     4201 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     4261 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     4321 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     4381 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     4441 tcttccgcgt ctccgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     4501 gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     4561 aagaaaaggg gggactggaa gggctaattc actcccaacg aagataagat ctgctttttg
     4621 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     4681 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     4741 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     4801 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     4861 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     4921 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     4981 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     5041 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     5101 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     5161 aggctttttt ggaggcctag acttttgcag agaccaaatt cgtaatcatg tcatagctgt
     5221 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     5281 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     5341 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     5401 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     5461 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     5521 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     5581 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     5641 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     5701 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     5761 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     5821 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     5881 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     5941 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     6001 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     6061 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     6121 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     6181 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     6241 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     6301 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     6361 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     6421 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     6481 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     6541 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     6601 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     6661 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     6721 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     6781 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     6841 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     6901 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     6961 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     7021 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     7081 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     7141 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     7201 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     7261 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     7321 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
     7381 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
     7441 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
     7501 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
     7561 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
     7621 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
     7681 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     7741 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
     7801 cacgacgttg taaaacgacg gccagtgcca agctgacgcg tgtagtctta tgcaatactc
     7861 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
     7921 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa
     7981 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta
     8041 tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg
     8101 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     8161 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     8221 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     8281 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     8341 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     8401 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     8461 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     8521 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     8581 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     8641 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     8701 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     8761 ccactgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     8821 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     8881 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     8941 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     9001 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     9061 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     9121 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     9181 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     9241 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     9301 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     9361 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     9421 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     9481 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     9541 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
//