| 质粒名称: | PCDH-METTL14-R298P |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 141113 |
| 存储实验室: | Jianjun Chen |
|
载体骨架基本信息 |
|
| 载体名称: | PCDH |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 嘌呤霉素 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | -- |
|
插入基因信息 |
|
| 插入基因名称: | HMETTL14 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | Substitution - Missense, position 298, R➞P |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | PCDH-METTL14-R298P was a gift from Jianjun Chen (Addgene plasmid # 141113 ; http://n2t.net/addgene:141113 ; RRID:Addgene_141113) |
| 参考文献部分: | METTL14 Inhibits Hematopoietic Stem/Progenitor Differentiation and Promotes Leukemogenesis via mRNA m(6)A Modification. Weng H, Huang H, Wu H, Qin X, Zhao BS, Dong L, Shi H, Skibbe J, Shen C, Hu C, Sheng Y, Wang Y, Wunderlich M, Zhang B, Dore LC, Su R, Deng X, Ferchen K, Li C, Sun M, Lu Z, Jiang X, Marcucci G, Mulloy JC, Yang J, Qian Z, Wei M, He C, Chen J. Cell Stem Cell. 2018 Feb 1;22(2):191-205.e9. doi: 10.1016/j.stem.2017.11.016. Epub 2017 Dec 28. 10.1016/j.stem.2017.11.016 PubMed 29290617 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS PCDH-METTL14-R298P 9590 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Overexpression of METTL14 mutant in mammalian cells..
ACCESSION .
VERSION .
KEYWORDS PCDH-METTL14-R298P
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9590)
AUTHORS Weng H, Huang H, Wu H, Qin X, Zhao BS, Dong L, Shi H, Skibbe J, Shen
C, Hu C, Sheng Y, Wang Y, Wunderlich M, Zhang B, Dore LC, Su R, Deng
X, Ferchen K, Li C, Sun M, Lu Z, Jiang X, Marcucci G, Mulloy JC,
Yang J, Qian Z, Wei M, He C, Chen J
TITLE METTL14 Inhibits Hematopoietic Stem/Progenitor Differentiation and
Promotes Leukemogenesis via mRNA m(6)A Modification.
JOURNAL Cell Stem Cell. 2018 Feb 1;22(2):191-205.e9. doi:
10.1016/j.stem.2017.11.016. Epub 2017 Dec 28.
PUBMED 29290617
REFERENCE 2 (bases 1 to 9590)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9590
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 52..168
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 258..461
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 412..432
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 458..482
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 541..567
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
promoter 1977..2188
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 2201..2469
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
regulatory 2498..2507
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 2528..3190
/codon_start=1
/product="green fluorescent protein 2 from Pontellina
plumata, also known as ppluGFP2 (Shagin et al., 2004)"
/label=CopGFP
/translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
CDS 3260..3313
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 3314..3913
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(3314..3333)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 3810..3830
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 3914..4502
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(3967..3987)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4385..4396)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 4576..4809
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 4881..5002
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4918..4937)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 4972..4991
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 5042..5177
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5104..5123
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(5210..5226)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5210..5226)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5223..5245)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5234..5250
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5258..5288)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5303..5324
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5441..5458)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5612..6200)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5692..5711)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6371..7231)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6994..7013
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7232..7336)
/gene="bla"
/label=AmpR promoter
primer_bind 7404..7422
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(7460..7482)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 7582..7601
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 7795..7817
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 7809..7826
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 7810..7826
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 7841..8067
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 8068..8248
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 8295..8420
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 8913..9146
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 9331..9375
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
ORIGIN
1 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag
61 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
121 catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat cgatactagt
181 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
241 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
301 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
361 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
421 gcggtaggcg tgtacggtgg gaggtttata taagcagagc tcgtttagtg aaccgtcaga
481 tcgcctggag acgccatcca cgctgttttg acctccatag aagattctag aaccaccatg
541 tacccatacg atgttccaga ttacgctgat agccgcttgc aggagatccg ggagcggcag
601 aagttacggc gacagctcct cgcgcagcag ttgggagctg aaagtgccga cagcattggt
661 gccgtgttaa atagcaaaga tgagcagaga gaaattgctg aaacaagaga aacttgcagg
721 gcttcctatg atacctctgc tccaaatgca aaacgtaagt atctggatga aggagagaca
781 gatgaagaca aaatggaaga atataaggat gaactagaaa tgcaacagga tgaagaaaat
841 ttgccatatg aagaagagat ttacaaagat tctagtactt ttcttaaggg aacacagagc
901 ttaaatcccc ataatgatta ctgccaacat tttgtagaca ctggacatag acctcagaat
961 ttcatcaggg atgtaggttt agctgacaga tttgaagaat atcctaaact gagggagctc
1021 atcaggctaa aggatgagtt aatagctaaa tctaacactc ctcccatgta cttacaagcc
1081 gatatagaag cctttgacat cagagaacta acacccaaat ttgatgtgat tcttctggaa
1141 ccccctttag aagaatatta cagagaaact ggcatcactg ctaatgaaaa atgctggact
1201 tgggatgata ttatgaagtt agaaattgat gagattgcag cacctcgatc atttattttt
1261 ctctggtgtg gttctgggga ggggttggac cttggaagag tgtgtttacg aaaatggggt
1321 tacagaagat gtgaagatat ttgttggatt aaaaccaata aaaacaatcc tgggaagact
1381 aagactttag atccaaaggc tgtctttcag agaacaaagg aacactgcct catggggatc
1441 aaaggaactg tgaagcctag cacagacggg gacttcattc atgctaatgt tgacattgac
1501 ttaattatca cagaagaacc tgaaattggc aatatagaaa aacctgtaga aatttttcat
1561 ataattgagc atttttgtct tggtagaaga cgccttcatc tatttggaag agatagtaca
1621 attcgaccag gctggctcac agttggacca acgcttacaa atagcaacta caatgcagaa
1681 acatatgcat cctatttcag tgctcctaat tcctacttga ctggttgtac agaagaaatt
1741 gagagacttc gaccaaaatc gcctcctccc aaatctaaat ctgaccgagg aggtggagct
1801 cccagaggtg gaggaagagg tggaacttct gctggccgtg gacgagaaag aaatagatct
1861 aacttccgag gagaaagagg tggctttaga gggggccgtg gaggagcaca cagaggtggc
1921 tttccacctc gataagcggc cgcgaaggat ctgcgatcgc tccggtgccc gtcagtgggc
1981 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg
2041 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
2101 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
2161 tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc
2221 acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc
2281 tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc
2341 gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac
2401 gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg
2461 ttacagatcc aagctgtgac cggcgcctac gctagacgcc accatggaga gcgacgagag
2521 cggcctgccc gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt
2581 cgagctggtg ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa
2641 gagcaccaaa ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg
2701 cttctaccac ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa
2761 caacggcggc tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt
2821 gagcttcagc taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac
2881 cggcttcccc gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt
2941 ggagcacctg caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag
3001 cctgcgcgac ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc
3061 catccacccc agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga
3121 gctgcacagc aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat
3181 cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc
3241 cggctccacc ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga
3301 gaatcccggc cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
3361 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
3421 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
3481 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
3541 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
3601 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
3661 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
3721 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
3781 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
3841 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
3901 gcccggtgcc tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
3961 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
4021 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
4081 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
4141 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
4201 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
4261 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
4321 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
4381 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
4441 tcttccgcgt ctccgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
4501 gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
4561 aagaaaaggg gggactggaa gggctaattc actcccaacg aagataagat ctgctttttg
4621 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
4681 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
4741 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
4801 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
4861 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
4921 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
4981 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
5041 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
5101 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
5161 aggctttttt ggaggcctag acttttgcag agaccaaatt cgtaatcatg tcatagctgt
5221 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
5281 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
5341 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
5401 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
5461 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
5521 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
5581 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
5641 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
5701 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
5761 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
5821 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
5881 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
5941 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
6001 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
6061 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
6121 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
6181 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
6241 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
6301 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
6361 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
6421 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
6481 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
6541 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
6601 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
6661 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
6721 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
6781 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
6841 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
6901 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
6961 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
7021 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
7081 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
7141 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
7201 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
7261 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
7321 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
7381 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
7441 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
7501 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
7561 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc
7621 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat
7681 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
7741 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
7801 cacgacgttg taaaacgacg gccagtgcca agctgacgcg tgtagtctta tgcaatactc
7861 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
7921 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa
7981 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta
8041 tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg
8101 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
8161 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
8221 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
8281 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
8341 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
8401 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
8461 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
8521 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
8581 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
8641 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
8701 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
8761 ccactgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
8821 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
8881 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
8941 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
9001 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
9061 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
9121 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
9181 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
9241 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
9301 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
9361 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
9421 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
9481 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
9541 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
//
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