pRSV-rev

价格:850元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Addgene
载体名称: pRSV-rev
质粒类型: 慢病毒包装载体;哺乳动物表达载体
高拷贝/低拷贝: 高拷贝
启动子: RSV U3 
克隆方法: 多克隆位点,限制性内切酶
载体大小: 4174bp
5' 测序引物及序列: pREP fwd:  GCTCGATACAATAAACGCC
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素(Ampicillian)
筛选标记: --
备注: pRSV-rev第三代慢病毒包装载体,与之配套的包装载体为pMD2.GpMDLg/pRRE
产品目录号: 12253
稳定性: 瞬表达
组成型: 组成型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pRSV-rev载体图谱



载体简介

Rev cDNA expressing plasmid in which the joined second and third exons of HIV-1 rev are under the 
transcriptional control of RSV U3 promoter.
Part of the 3rd generation packaging system. Please note that most of Trono lab lentiviral vectors 
can only be used with the 2nd generation packaging system.

载体序列

LOCUS       pRSV-Rev	4174 bp 	DNA	SYN
DEFINITION  pRSV-Rev
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="pRSV-Rev"
                     /mol_type="other DNA"
     promoter        143..172
                     /label="lac_promoter"
     misc_feature    186..208
                     /label="M13_pUC_rev_primer"
     promoter        207..225
                     /label="M13_reverse_primer"
     misc_feature    579..597
                     /label="pREP_fwd_primer"
     misc_feature    638..662
                     /label="LNCX_primer"
     misc_feature    978..1007
                     /label="HIV_Rev_NES"
     promoter        complement(1190..1206)
                     /label="M13_forward20_primer"
     rep_origin      1549..1855
                     /label="f1_origin"
     misc_feature    2123..2145
                     /label="pGEX_3_primer"
     promoter        2304..2332
                     /label="AmpR_promoter"
     gene            2374..3234
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             2374..3234
                     /label="ORF frame 1"

     rep_origin      3389..4008
                     /label="pBR322_origin"

ORIGIN
    1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
   61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
  121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
  181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gaattcgatg
  241 tacgggccag atatacgcgt atctgagggg actaggtgtg tttaggcgaa aagcggggct
  301 tcggttgtac gcggttagga gtcccctcag gattagtagt ttcgcttttg catagggagg
  361 gggaaatgta gtcttatgca atacacttgt agtcttgcaa catggtaacg atgagttagc
  421 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
  481 acgatcgtgc cttattagga aggcaacaga caggtctgac atggattgga cgaaccactg
  541 aattccgcat tgcagagata attgtattta agtgcctagc tcgatacaat aaacgccatt
  601 tgaccattca ccacattggt gtgcacctcc aagctcgagc tcgtttagtg aaccgtcaga
  661 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca
  721 gcctcccctc gaagctagtc gattaggcat ctcctatggc aggaagaagc ggagacagcg
  781 acgaagacct cctcaaggca gtcagactca tcaagtttct ctatcaaagc aacccacctc
  841 ccaatcccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
  901 agagacagat ccattcgatt agtgaacgga tccttagcac ttatctggga cgatctgcgg
  961 agcctgtgcc tcttcagcta ccaccgcttg agagacttac tcttgattgt aacgaggatt
 1021 gtggaacttc tgggacgcag ggggtgggaa gccctcaaat attggtggaa tctcctacaa
 1081 tattggagtc aggagctaaa gaatagtgct gttagcttgc tcaatgccac agctatagca
 1141 gtagctgagg ggacagatag ggttatagaa gtagtacaag aagcttggca ctggccgtcg
 1201 ttttacatga tctgagcctg ggagatctct ggctaactag ggaacccact gcttaagcct
 1261 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
 1321 aactagagat caggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
 1381 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
 1441 cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
 1501 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
 1561 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
 1621 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
 1681 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
 1741 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
 1801 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
 1861 aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg
 1921 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
 1981 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
 2041 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
 2101 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
 2161 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
 2221 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
 2281 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
 2341 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
 2401 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
 2461 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
 2521 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
 2581 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
 2641 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
 2701 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
 2761 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
 2821 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
 2881 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
 2941 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
 3001 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
 3061 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
 3121 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
 3181 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
 3241 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
 3301 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
 3361 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
 3421 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
 3481 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
 3541 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
 3601 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
 3661 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
 3721 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
 3781 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
 3841 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
 3901 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
 3961 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
 4021 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
 4081 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
 4141 gagcgcagcg agtcagtgag cgaggaagcg gaag
//

载体质粒图谱pdf版和相关资料下载

载体应用举例

分享到:

全部载体分类

相关载体

相关文章

相关问答

Copyright © 20012-2013 BIOFENG. 生物风 版权所有 Powered by Biofeng