pAAV-CA

价格:1100元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Addgene
载体名称: pAAV-CA
质粒类型: 腺病毒载体; Cre-LoxP重组酶系统
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: CAG (CMV enhancer/chicken β-actin promoter)
载体大小: 6060 bp
5' 测序引物及序列: XpAAVE-F (GCTAACCATGTTCATGCCTTC)
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素
筛选标记:
克隆菌株: Stbl3 或 NEB Stble
宿主细胞(系): 常规哺乳动物细胞系
备注: --
产品目录号: #69616
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 腺病毒

载体质粒图谱和多克隆位点信息

pAAV-CA载体图谱



pAAV-CA 多克隆位点

载体简介

载体序列

LOCUS       pAAV-CA	6060 bp 	DNA	SYN
DEFINITION  pAAV-CA
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6060
                     /organism="pAAV-CA"
                     /mol_type="other DNA"
     misc_feature    239..526
                     /label="CAG_enhancer"
     CDS             410..889
                     /label="ORF frame 2"
                     /translation="MPSTPPIDVNDGKWPAWHYAQYMTLWDFPTWQYIYVLVIAITMG
                     RGEPHVLLHSPHLPPLPTPNFVFIYFLIILCSDGGGGGGGARQAGRGGARGGAGRGGE
                     VRRQPIRAARSESFLLWRGGGGGGPIKSEARGGRESLRCLRPVPRSAPPRAARPGSD*"
     CDS             complement(998..1501)
                     /label="ORF frame 3"
                     /translation="MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCRHPPPG
                     TASPAPRPVRSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA
                     PPHRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRT
                     KGPPGAL*"
     misc_feature    1836..1855
                     /label="pCAG_F_primer"
                     /translation="MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCRHPPPG
                     TASPAPRPVRSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA
                     PPHRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRT
                     KGPPGAL*"
     CDS             2145..2888
                     /label="ORF frame 3"
                     /translation="MYAIRGYRMLELIDNQPLDYKICERLTGILNYVAPFTLCGYAAL
                     MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTC
                     QLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGKSSSFPWLLAYVATW
                     ILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPHRYRALLE
                     RSTGGIPVTPPQCLSWPWKLPLQCPPALS*"
     misc_feature    2189..2776
                     /label="WPRE"
                     /translation="MYAIRGYRMLELIDNQPLDYKICERLTGILNYVAPFTLCGYAAL
                     MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTC
                     QLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGKSSSFPWLLAYVATW
                     ILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPHRYRALLE
                     RSTGGIPVTPPQCLSWPWKLPLQCPPALS*"
     CDS             2290..2823
                     /label="ORF frame 1"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTERCSRDLRVASL*"
     terminator      2805..3283
                     /label="hGH_PA_terminator"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTERCSRDLRVASL*"
     rep_origin      3555..3861
                     /label="f1_origin"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTERCSRDLRVASL*"
     misc_feature    4129..4151
                     /label="pGEX_3_primer"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTERCSRDLRVASL*"
     promoter        4310..4338
                     /label="AmpR_promoter"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTERCSRDLRVASL*"
     gene            4380..5240
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP
                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF
                     LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP
                     PRIDTERCSRDLRVASL*"
     CDS             4380..5240
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      5395..6014
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
   61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
  121 actccatcac taggggttcc tgcggccgca cgcgtAAGCT GGGctgcagg aatctagtta
  181 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
  241 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
  301 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
  361 ggactattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
  421 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
  481 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggg
  541 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
  601 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
  661 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
  721 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
  781 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgtt gccttcgccc
  841 cgtgccccgc tccgcgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc
  901 ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa
  961 tgacggctcg tttcttttct gtggctgcgt gaaagcctta aagggctccg ggagggccct
 1021 ttgtgcgggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc
 1081 gtgcggcccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg
 1141 ctccgcgtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc gggggggctg
 1201 cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg
 1261 cgcggcggtc gggctgtaac ccccccctgc acccccctcc ccgagttgct gagcacggcc
 1321 cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg
 1381 ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg
 1441 gggaggggcg cggcggcccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca
 1501 ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctgTgc
 1561 ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggG cgaagcggtg
 1621 cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc gccgcCgtcc
 1681 ccttctccCt ctccagcctc ggggctgTcc gcGgggggac ggctgccttc gggggggacg
 1741 gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct ctgctaacca
 1801 tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggttA ttgtgctgtc
 1861 tcatcatttt ggcaaagaat tgatttgata cgtacggttg aattcaTTAC CTCGTATAGG
 1921 ATACTTTATA CGAAGTTATG CAGAATGGTA GCTGGATTGT AAGTACTATT AGCAATATGA
 1981 AACCTCTTAA TAACCTCGTA TAGCATACAT TATACGAAGT AATAgatcta agcttgatat
 2041 cgtcGACATA ACTTCGTATA AAGTATCCTA TACGAGGTAA TTTGCCTTAA CCCAGAAATT
 2101 ATCAGTACTA TTCTTTAGAA TGGTGCAAAG AATTACTTCG TATAATGTAT GCTATACGAG
 2161 GTTATCGCAT Gctagagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
 2221 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
 2281 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
 2341 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
 2401 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
 2461 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
 2521 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
 2581 aatcatcgtc ctttccttgg ctgctcgcct atgttgccac ctggattctg cgcgggacgt
 2641 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
 2701 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
 2761 gggccgcctc cccgcatcga taccgagcgc tgctcgagag atctacgggt ggcatccctg
 2821 tgacccctcc ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct
 2881 tgtcctaata aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatattat
 2941 ggggtggagg ggggtggtat ggagcaaggg gcaagttggg aagacaacct gtagggcctg
 3001 cggggtctat tgggaaccaa gctggagtgc agtggcacaa tcttggctca ctgcaatctc
 3061 cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc gagttgttgg gattccaggc
 3121 atgcatgacc aggctcagct aatttttgtt tttttggtag agacggggtt tcaccatatt
 3181 ggccaggctg gtctccaact cctaatctca ggtgatctac ccaccttggc ctcccaaatt
 3241 gctgggatta caggcgtgaa ccactgctcc cttccctgtc cttctgattt tgtaggtaac
 3301 cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg
 3361 cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc
 3421 cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat
 3481 tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg
 3541 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
 3601 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
 3661 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
 3721 ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat
 3781 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
 3841 tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag
 3901 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
 3961 cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct
 4021 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
 4081 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
 4141 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
 4201 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
 4261 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
 4321 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
 4381 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
 4441 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
 4501 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
 4561 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
 4621 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
 4681 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
 4741 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
 4801 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
 4861 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
 4921 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
 4981 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
 5041 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
 5101 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
 5161 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
 5221 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
 5281 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
 5341 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
 5401 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
 5461 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
 5521 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
 5581 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
 5641 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
 5701 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
 5761 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
 5821 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
 5881 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
 5941 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
 6001 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt

//

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