| 质粒名称: | pBABE-hLin28B |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 26358 |
| 存储实验室: | George Daley |
|
载体骨架基本信息 |
|
| 载体名称: | pBabe.puro |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 5169 bp |
| 完整质粒大小: | 5922 bp |
| 载体类型: | 哺乳细胞表达，Retroviral |
| 筛选标记: | 嘌呤霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | lin-28 homolog B |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 753 bp |
| 基因突变: | None |
| GenBank编号 | NM_001004317 |
| 克隆方法: | 限制性内切酶 |
| 5' 测序引物 | pBABE 5' primer |
|
文献引用方法 |
|
| 材料和方法部分: | pBABE-hLin28B was a gift from George Daley (爱迪基因 plasmid # 26358) |
| 参考文献部分: | Lin28 promotes transformation and is associated with advanced human malignancies. Viswanathan SR, Powers JT, Einhorn W, Hoshida Y, Ng TL, Toffanin S, O'Sullivan M, Lu J, Phillips LA, Lockhart VL, Shah SP, Tanwar PS, Mermel CH, Beroukhim R, Azam M, Teixeira J, Meyerson M, Hughes TP, Llovet JM, Radich J, Mullighan CG, Golub TR, Sorensen PH, Daley GQ. Nat Genet. 2009 Jul . 41(7):843-8. 10.1038/ng.392 PubMed 19483683 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBABE-hLin28B 5875 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pBABE-hLin28B
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5875)
AUTHORS Viswanathan SR, Powers JT, Einhorn W, Hoshida Y, Ng TL, Toffanin S,
O'Sullivan M, Lu J, Phillips LA, Lockhart VL, Shah SP, Tanwar PS,
Mermel CH, Beroukhim R, Azam M, Teixeira J, Meyerson M, Hughes TP,
Llovet JM, Radich J, Mullighan CG, Golub TR, Sorensen PH, Daley GQ
TITLE Lin28 promotes transformation and is associated with advanced human
malignancies.
JOURNAL Nat Genet. 2009 Jul . 41(7):843-8.
PUBMED 19483683
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pBABE-hLin28B|
FEATURES Location/Qualifiers
source 1..5875
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 120..589
/note="long terminal repeat from Moloney murine leukemia
virus"
CDS complement(810..1409)
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(893..913)
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
primer_bind 1390..1409
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
promoter complement(1419..1748)
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 1433..1568
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(1487..1506)
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind 1733..1753
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
CDS complement(2560..2583)
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS complement(2600..3016)
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind complement(2616..2632)
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
primer_bind complement(2648..2670)
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
misc_feature 3217..3416
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
LTR 3481..3950
/note="long terminal repeat from Moloney murine leukemia
virus"
promoter 3981..4085
/gene="bla"
/label=AmpR promoter
CDS 4086..4946
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4304..4323)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5106..5694
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5595..5614
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 5848..5865
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaactatg
61 gccatttaat gtaaatactt aagaaaaaaa accaaattaa ttttgataca tgctgcatgt
121 gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga
181 gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgact
241 cagtcaatcg gaggactggc gccccgagtg aggggttgtg ggctctttta ttgagctcgg
301 ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca
361 cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc
421 tgagggctgg accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg
481 cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttct tggccctgag
541 ccggggcagg aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatct
601 gttcctgacc ttgatctgaa cttctctatt ctcagttatg tatttttcca tgccttgcaa
661 aatggcgtta cttaagctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt
721 ttctggagac taaataaaat cttttatttt atcgatgcat ggggtcgtgc gctcctttcg
781 gtcgggcgct gcgggtcgtg gggcgggcgt caggcaccgg gcttgcgggt catgcaccag
841 gtgcgcggtc cttcgggcac ctcgacgtcg gcggtgacgg tgaagccgag ccgctcgtag
901 aaggggaggt tgcggggcgc ggaggtctcc aggaaggcgg gcaccccggc gcgctcggcc
961 gcctccactc cggggagcac gacggcgctg cccagaccct tgccctggtg gtcgggcgag
1021 acgccgacgg tggccaggaa ccacgcgggc tccttgggcc ggtgcggcgc caggaggcct
1081 tccatctgtt gctgcgcggc cagccgggaa ccgctcaact cggccatgcg cgggccgatc
1141 tcggcgaaca ccgcccccgc ttcgacgctc tccggcgtgg tccagaccgc caccgcggcg
1201 ccgtcgtccg cgacccacac cttgccgatg tcgagcccga cgcgcgtgag gaagagttct
1261 tgcagctcgg tgacccgctc gatgtggcgg tccggatcga cggtgtggcg cgtggcgggg
1321 tagtcggcga acgcggcggc gagggtgcgt acggccctgg ggacgtcgtc gcgggtggcg
1381 aggcgcaccg tgggcttgta ctcggtcatg gtaagctttt tgcaaaagcc taggcctcca
1441 aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca
1501 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
1561 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
1621 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
1681 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
1741 tgacacacat tccacagggt cgacttatgt ctttttcctt ttttgaactg aaggcccctt
1801 tttgctttgc tcttctggtg ctatagatga cttcgtggag gaagcttctt gaggtgacct
1861 gcctgaccgt tctgagatct ctgccctagc ctcctgagga aacggtggtg atgtacagcc
1921 atgcccgcct cccacttctc gagggagagt tgaagtgcat ggctgggatt ctgcttcctg
1981 tcttccctga gaactcgcgg gtggctgtgc aacattttta tgtgggcagt ttgccaccat
2041 gtgcatgatg ctctgacagt aatggcactt ctttggctga ggaggtagac tacattcctt
2101 agcatgatga tcaaggccac cacagttgta gcatctatct ccctttggtt ttcttttctg
2161 tagtgtcttc cctttgggtc ttctttcact tcctaaacag gggctcccac caggtcctgt
2221 tacccgtatt gactcaaggc ctttggaaga ttttttaaat gtgaattcca ctggttctcc
2281 ttcttttagg cttctaaatc cttccatgaa tagtttgctt tggtgtacaa atacatcgac
2341 tggaatatcc aaggggcttc cctctcggtt tatcatggag atgaatccaa atcccatgcg
2401 cacattgaac cacttacagt ggccagttcc gcgcaaaacc tgggattcct cctctgccgg
2461 ctccggcagc ttcccgggct cttctccacc acctttgcta gccccgcctt cggccattgg
2521 taccgatatc agatctatcg atgaattcgc ggccgcaagc ttgtcgtcat cgtctttgta
2581 gtccatggtg gatccggccg gcgcctagag aaggagtgag ggctggataa agggaggatc
2641 gaggcggggt cgaacgagga ggttcaaggg ggagagacgg ggcggatgga ggaagaggag
2701 gcggaggctt agggtgtaca aagggcttga cccagggagg ggggtcaaaa gccaaggctt
2761 cccaggtcac gatgtagggg acctggtctg ggtgtccatg cgggccaggt gaaaagacct
2821 tgatcttaac ctgggtgatg aggtctcggt taaaggtgcc gtctcgcggc catccgacgt
2881 taaaggttgg ccattctgca gagcagaagg taacccaacg tctcttcttg acatctaccg
2941 actggttgtg agcgatccgc tcgacatctt tccagtgacc taaggtcaaa cttaagggag
3001 tggtaacagt ctggccctaa ttttcagaca aatacagaaa cacagtcaga cagagacaac
3061 acagaacgat gctgcagcgc tgcagcagac aagacgcgcg gcttcggttc caaaccgaaa
3121 gcaaaaattc agacggaggc gggaactgtt ttaggttctc gtctcctacc agaaccacat
3181 atcctgacgg ggtcggattc cacatcgact cccttcctca ggtcgggcca caaaaacggc
3241 ccccaaagtc cctgggacgt ctcccagggt tgcggccggg tgttcagaac tcgtcagttc
3301 caccacgggt ccgccagata cagagctagt tagctaacta gtaccgacgc aggcgcataa
3361 aatcagtcat agacactaga caatcggaca gacacagata agttgctggc cagcttgcct
3421 cccggtggtg ggtcggtggt ccctgggcag gggtctcccg atcccggacg agcccccaaa
3481 tgaaagaccc ccgctgacgg gtagtcaatc actcagagga gaccctccca aggaacagcg
3541 agaccacaag tcggatgcaa ctgcaagagg gtttattgga tacacgggta cccgggcgac
3601 tcagtcaatc ggaggactgg cgccccgagt gaggggttgt gggctctttt attgagctcg
3661 gggagcagaa gcgcgcgaac agaagcgaga agcgaactga ttggttagtt caaataaggc
3721 acagggtcat ttcaggtcct tggggcaccc tggaaacatc tgatggttct ctagaaactg
3781 ctgagggctg gaccgcatct ggggaccatc tgttcttggc cctgagccgg ggcaggaact
3841 gcttaccaca gatatcctgt ttggcccata ttcagctgtt ccatctgttc ttggccctga
3901 gccggggcag gaactgctta ccacagatat cctgtttggc ccatattcag gctgcaggtg
3961 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
4021 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
4081 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
4141 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
4201 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
4261 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
4321 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
4381 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
4441 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
4501 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
4561 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
4621 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
4681 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
4741 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
4801 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
4861 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
4921 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
4981 ttgatttgcg gccgcaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
5041 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
5101 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
5161 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
5221 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
5281 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
5341 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
5401 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
5461 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
5521 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
5581 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
5641 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
5701 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
5761 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
5821 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaag
//
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