pENTR5-UbCp

价格:2900元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Invitrogen
载体名称: pENTR5-UbCp
质粒类型: Gateway载体;入门载体
高拷贝/低拷贝: 高拷贝
启动子:
克隆方法: 限制性内切酶;多克隆位点
载体大小: 2720 bp
5' 测序引物及序列: pENTR-F: CTACAAACTCTTCCTGTTAGTTAG
3' 测序引物及序列: pENTR-R: ATGGCTCATAACACCCCTTG
载体标签:
载体抗性: Kanamycin.html' target='_blank'>卡那霉素
真核筛选标记:
备注:
pENTR5-UbCp载体本身含有CCDb致死基因,能够使得普通的DH5alpha等细菌死亡,必须使用DB3.1感受态细胞进行质粒扩增;

构建Gateway系统入门克隆时需要使用ccdB敏感的 OmniMAX2 感受态细胞。
 
产品目录号: --
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pENTR5-UbCp载体图谱

载体简介

载体序列

LOCUS       Exported                3861 bp ds-DNA     circular SYN 13-AUG-2016
KEYWORDS    pENTR5-UbCp
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Biofeng Lab.
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     546..562
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    601..697
                     /gene="mutant version of attL"
                     /bound_moiety="LR Clonase(TM)"
                     /note="attL4"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        698..1906
                     /note="Ubc promoter"
                     /note="human Ubc promoter"
     protein_bind    1907..2030
                     /gene="mutant version of attR"
                     /bound_moiety="LR Clonase(TM)"
                     /note="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(2103..2121)
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2126..2142)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2255..3064
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /note="KanR"
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      3211..3799
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aaacggaaga
      121 gcgcccaata cgcaaaccgc cctctccccg cgcgttggcc gattcattaa atgcagctgg
      181 cacgacaggt tttcccgact ggaaagcggg cagtggagcg caacgcaatt aatacccgta
      241 cccgcttacc caggaaaagt ttgtaaaaac gcaaaaaagg ccatccctcc aggatggcct
      301 tctgcttaat ttggatgcct ggcaagttta tggggggggt ctgcccgcca ccctccgggc
      361 cgttgcttcc acaacgttca aatccgctcc ccgcggattt gtcctactca ggagagcgtt
      421 caccgacaaa caacagataa aacgaaaggc ccagttcccg actgagcctt tcgttttatt
      481 tgatgcctgg cagttcccta ctctcgcgtt aacgctagca tggatgtttt cccagtcacg
      541 acgttgtaaa acgacggcca gtcttaagct cgggcccgcg ttaacgctac catggagctc
      601 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa
      661 tgctttttta taatgccaac tttgtataga aaagttgggc ctcgcgccgg gttttggcgc
      721 ctcccgcggg cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagga
      781 gcgtcctgat ccttccgccc ggacgctcag gacagcggcc cgctgctcat aagactcggc
      841 cttagaaccc cagtatcagc agaaggacat tttaggacgg gacttgggtg actctagggc
      901 actggttttc tttccagaga gcggaacagg cgaggaaaag tagtcccttc tcggcgattc
      961 tgcggaggga tctccgtggg gcggtgaacg ccgatgatta tataaggacg cgccgggtgt
     1021 ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg
     1081 tgatcgtcac ttggtgagta gcgggctgct gggctggccg gggctttcgt ggccgccggg
     1141 ccgctcggtg ggacggaagc gtgtggagag accgccaagg gctgtagtct gggtccgcga
     1201 gcaaggttgc cctgaactgg gggttggggg gagcgcagca aaatggcggc tgttcccgag
     1261 tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca
     1321 tggtgggcgg caagaaccca aggtcttgag gccttcgcta atgcgggaaa gctcttattc
     1381 gggtgagatg ggctggggca ccatctgggg accctgacgt gaagtttgtc actgactgga
     1441 gaactcggtt tgtcgtctgt tgcgggggcg gcagttatgc ggtgccgttg ggcagtgcac
     1501 ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta
     1561 taatgcaggg tggggccacc tgccggtagg tgtgcggtag gcttttctcc gtcgcaggac
     1621 gcagggttcg ggcctagggt aggctctcct gaatcgacag gcgccggacc tctggtgagg
     1681 ggagggataa gtgaggcgtc agtttctttg gtcggtttta tgtacctatc ttcttaagta
     1741 gctgaagctc cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt
     1801 ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt aattttcagt gttagactag
     1861 taaattgtcc gctaaattct ggccgttttt ggcttttttg ttagaccaag tttgtacaaa
     1921 aaagttgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat
     1981 aaaaaacaga ctacataata ctgtaaaaca caacatatgc agtcactatg aatcaactac
     2041 ttagatggta ttagtgacct gtagaattcg agctctagag ctgcagggcg gccgcgatat
     2101 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc
     2161 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact
     2221 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc
     2281 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
     2341 taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga
     2401 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag
     2461 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc
     2521 tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga
     2581 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt
     2641 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca
     2701 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa
     2761 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga
     2821 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
     2881 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat
     2941 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
     3001 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt
     3061 ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga
     3121 cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc
     3181 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtactctg
     3241 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3301 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     3361 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     3421 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     3481 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     3541 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     3601 gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     3661 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     3721 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3781 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3841 ctggcctttt gctcacatgt t
//

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