| 质粒名称: | pcDNA5FRT/Flag-OGT1 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 29760 |
| 存储实验室: | Joan Conaway |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA5FRT |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5100 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | OGT1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 3141 bp |
| 基因突变: | N764D and K1010E |
| GenBank编号 | NP_858059.1 NM_181672 |
| 启动子 | -- |
| 标签/融合蛋白: | Flag（插入N端子） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | T7 |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pcDNA5FRT/Flag-OGT1 was a gift from Joan Conaway (Addgene plasmid # 29760 ; http://n2t.net/addgene:29760 ; RRID:Addgene_29760) |
| 参考文献部分: | Subunit composition and substrate specificity of a MOF-containing histone acetyltransferase distinct from the male-specific lethal (MSL) complex. Cai Y, Jin J, Swanson SK, Cole MD, Choi SH, Florens L, Washburn MP, Conaway JW, Conaway RC. J Biol Chem. 2010 Feb 12. 285(7):4268-72. 10.1074/jbc.C109.087981 PubMed 20018852 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pcDNA5FRT/Flag-OGT1 8193 bp ds-DNA circular SYN 07-APR-2020
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pcDNA5FRT/Flag-OGT1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8193)
AUTHORS Cai Y, Jin J, Swanson SK, Cole MD, Choi SH, Florens L, Washburn MP,
Conaway JW, Conaway RC
TITLE Subunit composition and substrate specificity of a MOF-containing
histone acetyltransferase distinct from the male-specific lethal
(MSL) complex.
JOURNAL J Biol Chem. 2010 Feb 12. 285(7):4268-72.
PUBMED 20018852
REFERENCE 2 (bases 1 to 8193)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 7, 2020 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8193
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(54..73)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 245..624
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 625..828
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 779..799
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 873..892
/label=T7
/note="T7 promoter, forward primer"
promoter 873..891
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 917..940
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
primer_bind complement(4155..4172)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 4161..4385
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(4518..4537)
/label=F1ori-R
/note="F1 origin, reverse primer"
protein_bind 4669..4716
/label=FRT
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
CDS 4724..5746
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
"
polyA_signal 5876..5997
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5913..5932)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5967..5986
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(6046..6062)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6046..6062)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(6059..6081)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 6070..6086
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6094..6124)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6139..6160
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(6277..6294)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6448..7036)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6528..6547)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7207..8067)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7830..7849
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(8068..8172)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc
61 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
121 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
181 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
241 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
301 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
361 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
421 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
481 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
541 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
601 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
661 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
721 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
781 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact
841 agagaaccca ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa
901 gctggctagc atgccagact acaaggacga tgatgacaag ctcgacatgg cgtcttccgt
961 gggcaacgtg gccgacagca cagggttagc tgagttggca catcgagaat atcaggcagg
1021 agattttgag gcagctgaga gacactgcat gcagctctgg agacaagagc cagacaatac
1081 tggtgtgctt ttattacttt catctataca cttccagtgt cgaaggctgg acagatctgc
1141 tcactttagc actctggcaa ttaaacagaa cccccttctg gcagaagctt attcgaattt
1201 ggggaatgtg tacaaggaaa gagggcagtt gcaggaggca attgagcatt atcgacatgc
1261 attgcgtctc aaacctgatt tcatcgatgg ttatattaac ctggcagccg ccttggtagc
1321 agcgggtgac atggaagggg cagtacaagc ttacgtctct gctcttcagt acaatcctga
1381 tttgtactgt gttcgcagtg acctggggaa cctgctcaaa gccctgggtc gcttggaaga
1441 agccaaggca tgttatttga aagcaattga gacgcaaccg aactttgcag tagcttggag
1501 taatcttggc tgtgttttca atgcacaagg ggaaatttgg cttgcaattc atcactttga
1561 aaaggctgtc acccttgacc caaactttct ggatgcttat atcaatttag gaaatgtctt
1621 gaaagaggca cgcatttttg acagagctgt ggcagcttat cttcgtgccc taagtttgag
1681 tccaaatcac gcagtggtgc acggcaacct ggcttgtgta tactatgagc aaggcctgat
1741 agatctggca atagacacct acaggcgggc tatcgaacta caaccacatt tccctgatgc
1801 ttactgcaac ctagccaatg ctctcaaaga gaagggcagt gttgctgaag cagaagattg
1861 ttataataca gctctccgtc tgtgtcccac ccatgcagac tctctgaata acctagccaa
1921 tatcaaacga gaacagggaa acattgaaga ggcagttcgc ttgtatcgta aagcattaga
1981 agtcttccca gagtttgctg ctgcccattc aaatttagca agtgtactgc agcagcaggg
2041 aaaactgcag gaagctctga tgcattataa ggaggctatt cgaatcagtc ctacctttgc
2101 tgatgcctac tctaatatgg gaaacactct aaaggagatg caggatgttc agggagcctt
2161 gcagtgttat acgcgtgcca tccaaattaa tcctgcattt gcagatgcac atagcaatct
2221 ggcttccatt cataaggatt cagggaatat tccagaagcc atagcttctt accgcacggc
2281 tctgaaactt aagcctgatt ttcctgatgc ttattgtaac ttggctcatt gcctgcagat
2341 tgtctgtgat tggacagact atgatgagcg aatgaagaag ttggtcagta ttgtggctga
2401 ccagttagag aagaataggt tgccttctgt gcatcctcat catagtatgc tatatcctct
2461 ttctcatggc ttcaggaagg ctattgctga gaggcacggc aacctgtgct tagataagat
2521 taatgttctt cataaaccac catatgaaca tccaaaagac ttgaagctca gtgatggtcg
2581 gctgcgtgta ggatatgtga gttccgactt tgggaatcat cctacttctc accttatgca
2641 gtctattcca ggcatgcaca atcctgataa atttgaggtg ttctgttatg ccctgagccc
2701 agacgatggc acaaacttcc gagtgaaggt gatggcagaa gccaatcatt tcattgatct
2761 ttctcagatt ccatgcaatg gaaaagcagc tgatcgcatc catcaggatg gaattcatat
2821 ccttgtaaat atgaatggct atactaaggg cgctcgaaat gagctttttg ctctcaggcc
2881 agctcctatt caggcaatgt ggctgggata ccctgggacg agtggtgcgc ttttcatgga
2941 ttatattatc actgatcagg aaacttcgcc agctgaagtt gctgagcagt attccgagaa
3001 attggcttat atgccccaca ctttttttat tggtgatcat gctaatatgt tccctcacct
3061 gaagaaaaaa gcagtcatcg attttaagtc caatgggcac atttatgaca atcggatagt
3121 tctgaatggc atcgacctca aagcatttct tgatagtcta ccagatgtga aaattgtcaa
3181 gatgaagtgt cctgatggag gagacgatgc agatagcagt aacacagctc ttaatatgcc
3241 tgttattcct atgaatacta ttgcagaagc agttattgaa atgattaacc gaggacagat
3301 tcaaataaca attaatggat tcagtattag caatggactg gcaactactc agatcaacaa
3361 taaggctgca actggagagg aggttccccg taccattatt gtaaccaccc gttctcagta
3421 cgggttacca gaagatgcca tcgtatactg taactttaat cagttgtata aaattgaccc
3481 ttctactttg cagatgtggg caaacattct gaagcgtgtt cccaatagtg tactctggct
3541 gttgcgtttt ccagcagtag gagaacctaa tattcaacag tatgcacaga acatgggcct
3601 gccccagaac cgtatcattt tttcacctgt tgctcctaaa gaggaacacg tcaggagagg
3661 ccagctggct gatgtctgct tggacactcc actctgtaat gggcacacca cagggatgga
3721 tgtcctctgg gcagggaccc ccatggtgac tatgccagga gagactcttg cttctcgagt
3781 tgcagcatcc cagctcactt gcttaggttg tcttgagctt attgctaaaa acagacaaga
3841 atatgaagac atagctgtga agctgggaac tgatctagaa tacctgaaga aagttcgtgg
3901 caaagtctgg aagcaaagaa tatctagccc tctgttcaac accgaacaat acacaatgga
3961 actagagcgg ctctatctac agatgtggga gcattatgca gccggcaaca aacctgacca
4021 catgattaag cctgttgaag tcactgagtc agcataagga tccactagtc cagtgtggtg
4081 gaattctgca gatatccagc acagtggcgg ccgctcgatc gagtctagag ggcccgttta
4141 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
4201 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
4261 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
4321 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
4381 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
4441 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
4501 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
4561 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
4621 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtacctaga agttcctatt
4681 ccgaagttcc tattctctag aaagtatagg aacttccttg gccaaaaagc ctgaactcac
4741 cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
4801 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
4861 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt
4921 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct
4981 gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga
5041 actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct
5101 tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg
5161 gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga
5221 cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga
5281 ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga
5341 caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata
5401 cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg
5461 ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct
5521 ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc
5581 ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac
5641 acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga
5701 tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaatagcacg tactacgaga
5761 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
5821 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
5881 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
5941 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
6001 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
6061 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
6121 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
6181 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
6241 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
6301 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
6361 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
6421 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
6481 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
6541 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
6601 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
6661 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
6721 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
6781 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
6841 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
6901 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
6961 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
7021 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
7081 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
7141 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
7201 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
7261 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
7321 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
7381 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
7441 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
7501 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
7561 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
7621 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
7681 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
7741 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
7801 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
7861 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
7921 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
7981 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
8041 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
8101 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
8161 aggggttccg cgcacatttc cccgaaaagt gcc
//
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