pShuttle-IRES-hrGFP-2

价格:2000元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Agilent
载体名称: pShuttle-IRES-hrGFP-2
质粒类型: 腺病毒载体;双顺反子载体;荧光报告载体
高拷贝/低拷贝: 低拷贝
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 8.9 kb
5' 测序引物及序列: pShuttle-IRES-hrGFP-1-F: CTCACGGGGATTTCCAAGTC
3' 测序引物及序列: pShuttle-IRES-hrGFP-1-R: ATGCAGTCGTCGAGGAATTG
载体标签: --
载体抗性: 卡那霉素
备注: 载体内包含有IRES序列和hrGFP序列。
产品目录号: 240082
稳定性: 稳表达
组成型: 组成型
病毒/非病毒: 腺病毒

载体质粒图谱和多克隆位点信息

pShuttle-IRES-hrGFP-2载体图谱


pShuttle-IRES-hrGFP-2多克隆位点
pShuttle-IRES-hrGFP-2载体特征

载体简介

Two additional shuttle vectors, pShuttle-IRES-hrGFP-1 (Catalog #240081) and pShuttle-IRES-hrGFP-2 (Catalog #240082) are available separately. Both of these vectors contain the CMV promoter and a dicistronic expression cassette in which the multiple cloning site (MCS) is followed by the EMCV-IRES, which directs translation of a humanized recombinant green fluorescent protein (hrGFP) from a novel marine organism as a second open reading frame. This design allows the expression of the gene of interest (up to 5.2 kb) to be monitored at the single-cell level due to expression of the hrGFP on the same transcript. The gene of interest may be fused to three contiguous copies of either the FLAG® epitope (pShuttle-IRES-hrGFP-1) or the HA epitope (pShuttle-IRES-hrGFP-2).

载体序列

载体质粒图谱pdf版和相关资料下载

载体应用举例

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