| 质粒名称: | pET28a-MH6-RspCas13d_RspCasWYL1 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 108304 |
| 存储实验室: | Arbor Biotechnologies |
|
载体骨架基本信息 |
|
| 载体名称: | pET-28a(+) |
| 载体出品公司: | Novagen |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 5390 bp |
| 完整质粒大小: | 9420 bp |
| 载体类型: | 细菌表达，CRISPR |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | RspCas13d |
| 物种来源: | Synthetic |
| 插入大小: | 2763 bp |
| 启动子 | lac |
| 标签/融合蛋白: | mH6 tag（插入件上的N端子） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRI(未破坏) |
| 3' 克隆位点: | EcoRI(未破坏) |
| 5' 测序引物 | ATGGCCAAAAAGAACAAAATGAAACC |
|
文献引用方法 |
|
| 材料和方法部分: | pET28a-MH6-RspCas13d_RspCasWYL1 was a gift from Arbor Biotechnologies (Addgene plasmid # 108304 ; http://n2t.net/addgene:108304 ; RRID:Addgene_108304) |
| 参考文献部分: | Cas13d Is a Compact RNA-Targeting Type VI CRISPR Effector Positively Modulated by a WYL-Domain-Containing Accessory Protein. Yan WX, Chong S, Zhang H, Makarova KS, Koonin EV, Cheng DR, Scott DA. Mol Cell. 2018 Mar 9. pii: S1097-2765(18)30173-4. doi: 10.1016/j.molcel.2018.02.028. 10.1016/j.molcel.2018.02.028 PubMed 29551514 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pET28a-MH6-RspCas13d_RspCasWYL1 9416 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Expresses E. coli codon optimized RspCas13d and RspCasWYL1 in the
pET28a backbone..
ACCESSION .
VERSION .
KEYWORDS pET28a-MH6-RspCas13d_RspCasWYL1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9416)
AUTHORS Yan WX, Chong S, Zhang H, Makarova KS, Koonin EV, Cheng DR, Scott DA
TITLE Cas13d Is a Compact RNA-Targeting Type VI CRISPR Effector Positively
Modulated by a WYL-Domain-Containing Accessory Protein.
JOURNAL Mol Cell. 2018 Mar 9. pii: S1097-2765(18)30173-4. doi:
10.1016/j.molcel.2018.02.028.
PUBMED 29551514
REFERENCE 2 (bases 1 to 9416)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9416
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 1..20
/label=T7
/note="T7 promoter, forward primer"
promoter 1..19
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 20..44
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter 53..82
/label=lac promoter
/note="promoter for the E. coli lac operon"
RBS 102..124
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 156..173
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
RBS 2956..2978
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 3010..3027
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
promoter 4222..4256
/label=J23119 promoter
/note="bacterial promoter (Registry of Standard Biological
Parts BBa_J23119)"
primer_bind complement(4351..4369)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 4365..4412
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
rep_origin 4449..4904
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4536..4555)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4746..4767
/label=F1ori-F
/note="F1 origin, forward primer"
CDS complement(4996..5811)
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
primer_bind 5719..5738
/label=Kan-R
/note="Kanamycin resistance gene, reverse primer"
primer_bind 5803..5822
/label=pENTR-R
/note="pENTR vectors, reverse primer"
rep_origin 5933..6521
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6422..6441
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 6675..6692
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 6707..6846
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(6772..6791)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 6932..6954
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
CDS complement(6948..7139)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
CDS complement(7948..9030)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
primer_bind 8992..9011
/label=LacI-R
/note="LacI, reverse primer"
promoter complement(9031..9108)
/gene="lacI"
/label=lacI promoter
primer_bind 9314..9333
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
ORIGIN
1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gatttacact
61 ttatgcttcc ggctcgtatg tttctagaga attcaaataa ttttgtttaa ctttaagaag
121 gagatttaaa tatgaaaatc gaagaaggta aaggtcacca tcaccatcac cacatggcca
181 aaaagaacaa aatgaaaccg cgtgaactgc gtgaagcaca gaaaaaagca cgtcagctga
241 aagcagcaga aattaacaat aatgcagcac cggcaattgc agcaatgcct gcagcagaag
301 ttattgcacc ggttgcagaa aaaaagaaaa gcagcgttaa agcagccggt atgaaaagca
361 ttctggtgag caaaaacaaa atgtatatca ccagctttgg caaaggtaat agcgcagttc
421 tggaatatga agtggataac aacgattaca atcagaccca gctgagcagc aaaggtagca
481 gcaatattga gctgcgtggt gttaatgaag tgaacattac ctttagcagc aaacacggtt
541 ttgaaagcgg tgttgaaatc aataccagca atccgacaca tcgtagcggt gaaagcagtc
601 cggttcgtgg tgatatgctg ggcctgaaaa gcgaactgga aaaacgcttt tttggcaaaa
661 ccttcgatga caacattcat atccagctga tctataacat cctggacatc gaaaaaattc
721 tggccgttta tgtgaccaac attgtgtatg cactgaataa catgctgagc attaaagata
781 gcgagagcta tgatgatttc atgggttatc tgagcgcacg caatacctat gaagttttta
841 cccatccgga taaaagcaac ctgagcgata aagcaaaagg caacatcaaa aaatccttta
901 gcaccttcaa cgacctgctg aaaaccaaac gtctgggtta ttttggtctg gaagaaccga
961 aaacaaaaga tacccgtgtt agccaggcat acaaaaaacg tgtttatcac atgctggcaa
1021 ttgtgggtca gattcgtcag agcgtgtttc atgataaaag tagcaaactg gatgaggacc
1081 tgtatagctt tatcgatatc atcgattccg aatatcgtga aaccctggat tatctggttg
1141 atgaacgttt tgacagcatc aacaaaggtt ttatccaggg caacaaagtg aatattagcc
1201 tgctgatcga tatgatgaaa ggctatgaag ccgatgatat tatccgcctg tattatgatt
1261 ttatcgtgct gaaaagccag aagaatctgg gctttagtat caaaaaactg cgcgagaaaa
1321 tgctggatga atatggcttt cgcttcaaag ataaacagta tgatagcgtg cgtagcaaga
1381 tgtataaact gatggatttt ctgctgttct gcaactatta tcgcaatgat gttgttgccg
1441 gtgaagcact ggttcgtaaa ctgcgtttta gcatgaccga tgatgaaaaa gaaggtattt
1501 acgcagacga agcaagcaaa ctgtggggta aatttcgtaa cgattttgag aacattgccg
1561 accatatgaa cggtgatgtt attaaagaac tgggcaaagc cgatatggat ttcgatgaaa
1621 aaatactgga cagcgagaaa aaaaacgcaa gcgatctgct gtatttcagc aagatgattt
1681 atatgctgac ctatttcctg gatggcaaag aaattaatga tctgctgacc acgctgatta
1741 gcaaatttga taacatcaaa gaatttctga aaatcatgaa aagctcagcc gttgatgttg
1801 aatgtgaact gaccgcaggt tataaactgt ttaatgatag ccagcgcatt accaacgaac
1861 tgttcattgt taaaaacatt gcgagcatgc gtaaaccggc aagcagcgca aaactgacca
1921 tgtttcgtga tgcactgacc attctgggta ttgatgataa cattaccgat gatcgcatta
1981 gcgaaatcct gaagctgaaa gaaaaaggta aaggtattca tggcctgcgc aactttatta
2041 ccaataacgt gattgaaagc tcccgctttg tgtacctgat caaatatgca aatgcccaga
2101 aaattcgcaa agtggcgaaa aatgaaaagg tggtgatgtt tgtgttaggt ggtattccgg
2161 atacacagat tgaacgctat tacaaaagct gcgttgaatt tccggatatg aacagcagcc
2221 tggaagttaa acgtagtgaa ctggcacgta tgatcaaaaa catcagcttc gacgatttca
2281 agaacgttaa acagcaggca aaaggtcgtg aaaatgttgc aaaagaacgt gccaaagcag
2341 tgattggtct gtatctgacc gttatgtatc tgctggttaa aaatctggtt aacgtgaatg
2401 cccgttatgt gattgcaatt cattgtctgg aacgtgattt cggcctgtat aaagaaatca
2461 ttccggaact ggcaagcaag aacctgaaaa atgattatcg tattctgagt cagaccctgt
2521 gtgaactgtg tgataaaagc ccgaacctgt ttctgaagaa aaacgaacgt ctgcgcaaat
2581 gcgttgaggt ggatattaac aatgcagata gcagcatgac ccgcaaatat cgtaattgta
2641 ttgcacatct gacagttgtg cgcgaactga aagaatatat tggtgatatt cgtaccgtgg
2701 acagctactt tagcatctat cattatgtta tgcagcggtg tattaccaaa cgcgaaaatg
2761 ataccaaaca agaagagaaa atcaaatacg aggacgatct gcttaaaaac cacggctata
2821 ccaaagattt tgtgaaagcc ctgaatagcc cgttcggtta taacattccg cgtttcaaaa
2881 atctgagcat cgagcagctg tttgatcgta atgaatatct gacggaaaaa taataagaat
2941 tcggatccaa ataattttgt ttaactttaa gaaggagatt taaatatgaa aatcgaagaa
3001 ggtaaaggtc accatcacca tcaccacatg ctgattccgc ctagcacctt tctgccgaaa
3061 cgtgataaaa atgttccgta tattgcagag gttcagagca ttccgctgag cccgagcgca
3121 tatagcgtta ttatcaaaga taagagcatc tttgaaacca gcctgagtcc gaatggtagc
3181 gttagcatga gcagctttct gaccagcatt tttgatagcg cctatatcgc aagcctgaaa
3241 tacaaaagcg acgacaacta taaatacatt ggtattccgc tgctgaacgc ctttgttaaa
3301 tggcagattg aagaaattga tgatggcctg gatgacaaga gcaaagaaat cattaaaagc
3361 tatctgatca gcaagctgag cgccaaatat gaaaaaacca aaaccgaaaa tgccgttcgt
3421 gttcgtctga gcatttgtcg tgatctgtat gataccctga gcagcgacga tctgtattat
3481 gaaaataaag tgtatagcag caccctgcgt cgttttctga aagcagttta tgaagattat
3541 gcactgctga gcgattgtga acgtgaacgt ctgatttttg ccgataacat catcaaaatc
3601 aacgaagtga ttaagcagaa tggcagccgt tattatagct tcatttatgc ctatagcaac
3661 atgtacagcc gtgaaaaacg tcgtattcgc ctgattccgt atcgtattgt ttccgatgag
3721 tacaaaatgt ataactacct ggtttgtctg agcgacgaaa aaagcgcagg taaagaattt
3781 aaagcagata gctgtcgtat tagccgtctg agcggtctga gtattgcaga aaaactgagc
3841 cagaaagaat atagcagcgt gaccgaatat gaacgcctga aagaagttca cgttaaaagc
3901 gttaaacacc tgttaagcga tccgcgtttt ggtagtgatg aaagcgatat tagcaaagtg
3961 tatctgaccg aaaaaggcgt ggaaatgttt ggcaaaattc tgtatcagcg tccgattctg
4021 aaaggcaatg agaaaccgaa acctaatgcc gtgaatgaat ttatcagccc tccgattcag
4081 gtgaaatact actttaacaa attcggcaaa gacggcgtta ttctgagccc gtctgatagc
4141 tttgaagaga tgcgtaccct gtatgttgaa ggtgccgaag catataatcg tgaagttgaa
4201 atgtaataag gatccctcga gttgacagct agctcagtcc taggtataat gctagcagag
4261 accatggcca ccggtctctg ctaaagatat gaggagctca aactcgagct gctaacaaag
4321 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
4381 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggattgg
4441 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
4501 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
4561 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
4621 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
4681 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
4741 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
4801 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
4861 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
4921 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
4981 ctcatgaatt aattcttaga aaaactcatc gagcatcaaa tgaaactgca atttattcat
5041 atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc
5101 accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc
5161 aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc
5221 accatgagtg acgactgaat ccggtgagaa tggcaaaagt ttatgcattt ctttccagac
5281 ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt
5341 attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt
5401 acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc
5461 acctgaatca ggatattctt ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt
5521 gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa
5581 ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt
5641 gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc
5701 acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt
5761 ggaatttaat cgcggcctag agcaagacgt ttcccgttga atatggctca taacacccct
5821 tgtattactg tttatgtaag cagacagttt tattgttcat gaccaaaatc ccttaacgtg
5881 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
5941 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
6001 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
6061 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
6121 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
6181 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
6241 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
6301 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
6361 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
6421 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
6481 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
6541 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
6601 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
6661 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
6721 ttctccttac gcatctgtgc ggtatttcac accgcaatgg tgcactctca gtacaatctg
6781 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg
6841 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
6901 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
6961 ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc
7021 gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt
7081 aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact
7141 gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag
7201 aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag
7261 ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc
7321 cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg
7381 cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca
7441 cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg
7501 cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag
7561 cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg
7621 gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga
7681 gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag
7741 cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc
7801 atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag
7861 gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg
7921 agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
7981 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
8041 cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg
8101 gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg
8161 tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac
8221 taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag
8281 cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg
8341 catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg
8401 aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga
8461 acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac
8521 gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga
8581 gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg
8641 gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac
8701 cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc
8761 cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag
8821 actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg
8881 gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga
8941 aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc
9001 tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg
9061 gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac
9121 gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga
9181 gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca
9241 cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc
9301 gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg
9361 tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
//
