pLVX-DD-AmCyan1 Reporter

价格:2500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pLVX-DD-AmCyan1 Reporter
质粒类型: 慢病毒载体;荧光报告载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子:
载体大小: 8494 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: 青色荧光蛋白DD-AmCyan1
载体抗性: 氨苄青霉素
筛选标记: 嘌呤霉素(Puromycin)
克隆菌株: Stbl3
宿主细胞(系): 常规细胞系,293、CV-1、CHO等
备注: pLVX-DD-AmCyan1 Reporter载体没有启动子;
DD-AmCyan1与AmCyan1相比,N端融合了ProteoTuner destabilization domain (DD)去稳定结构域,
导致AmCyan1在细胞内很快被蛋白酶降解,降低了背景荧光,是研究启动子活性的理想报告基因。
产品目录号: 631749
稳定性: 稳表达
组成型/诱导型: --
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLVX-DD-AmCyan1 Reporter载体图谱




pLVX-DD-AmCyan1 Reporter 载体特征

载体简介

pLVX-DD-AmCyan1 Reporter is a promoterless reporter vector that allows the functional analysis of different promoters
and promoter/enhancer combinations inserted into its multiple cloning site (MCS). The vector encodes the reporter protein
DD-AmCyan1, a ligand-dependent, destabilized cyan fluorescent protein that minimizes background fluorescence from
leaky promoters. A promoter must be cloned into the MCS, located upstream of the DD-AmCyan1 coding sequence.
Without the addition of a functional promoter, the vector will not express DD-AmCyan1.

Location of Features
5' LTR: 1–635
PBS (primer binding site): 636–653
Ψ (packaging signal): 685–822
RRE (Rev-response element): 1303–1536
cPPT/CTS (central polypurine tract/central termination sequence): 2028–2151
MCS (multiple cloning site): 2195–2234
DD (FKBP-L106P destabilization domain): 2247–2570
AmCyan1 (Anemonia majano cyan fluorescent protein): 2577–3263
PPGK (phosphoglycerate kinase promoter): 3274–3782
Puror(puromycin resistance gene): 3803–4402
WPRE (woodchuck hepatitis virus posttranscriptional regulatory element): 4416–5007
3' LTR: 5211–5847
pUC origin of replication: 6317–6987 (complementary)
Ampr(ampicillin resistance gene; β-lactamase): 7132–8128 (complementary)

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