pDD-tdTomato Reporter

价格:3000元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Clontech
载体名称: pDD-tdTomato Reporter
质粒类型: 无启动子载体;荧光报告载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子:
载体大小: 5351 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: DD tag  (N-端)
载体抗性: 卡那霉素
筛选标记: 新霉素(Neomycin)
克隆菌株: DH5α, HB101
宿主细胞(系): 常规细胞系(293、CV-1、CHO等)
备注: 哺乳动物载体pDD-tdTomato Reporter是研究启动子及其它顺式转录元件活性的载体;
DD-tdTomato与tdTomato相比,N端融合了ProteoTuner destabilization domain (DD)去稳定结构域,导致tdTomato在细胞内很快被蛋白酶降解,降低了背景荧光,是研究启动子活性的理想报告基因。。
产品目录号: 632193
稳定性: 稳表达 或 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pDD-tdTomato Reporter载体图谱



pDD-tdTomato Reporter 载体特征

载体简介

载体序列

LOCUS       pDD-tdTomato_Reporter   5351 bp    DNA   circular             8-MAR-2011
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No.  632193,632190
FEATURES             Location/Qualifiers
     CDS             3087..3881
                     /label=Kan(R)/Neo(R)
     rep_origin      4466..5109
                     /label=pUC\origin
     CDS             97..420
                     /label=DD
     CDS             427..1854
                     /label=tdTomato
     rep_origin      complement(2107..2562)
                     /label=f1\origin
     rep_origin      2903..3041
                     /label=SV40\origin
BASE COUNT     1269 a      1490 c      1460 g      1132 t 
ORIGIN
        1 tagttattac tagcgctacc ggactcagat ctcgagctca agcttcgaat tctgcagtcg 
       61 acggtaccgc gggcccggga tccaccggtc gccaccatgg gagtgcaggt ggaaaccatc 
      121 tccccaggag acgggcgcac cttccccaag cgcggccaga cctgtgtggt gcactacacc 
      181 gggatgcttg aagatggaaa gaaagtcgat tcctcccggg acagaaacaa gccctttaag 
      241 tttatgctag gcaagcagga ggtgatccga ggctgggaag aaggggttgc ccagatgagt 
      301 gtgggtcaga gagccaaact gactatatct ccagattatg cctatggtgc cactgggcac 
      361 ccaggcatca tcccaccaca tgccactctc gtcttcgatg tggagcttct aaaaccggaa 
      421 agatctatgg tgagcaaggg cgaggaggtc atcaaagagt tcatgcgctt caaggtgcgc 
      481 atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc 
      541 tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc 
      601 tgggacatcc tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc 
      661 gacatccccg attacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg 
      721 aacttcgagg acggcggtct ggtgaccgtg acccaggact cctccctgca ggacggcacg 
      781 ctgatctaca aggtgaagat gcgcggcacc aacttccccc ccgacggccc cgtaatgcag 
      841 aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg 
      901 aagggcgaga tccaccaggc cctgaagctg aaggacggcg gccactacct ggtggagttc 
      961 aagaccatct acatggccaa gaagcccgtg caactgcccg gctactacta cgtggacacc 
     1021 aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgctcc 
     1081 gagggccgcc accacctgtt cctggggcat ggcaccggca gcaccggcag cggcagctcc 
     1141 ggcaccgcct cctccgagga caacaacatg gccgtcatca aagagttcat gcgcttcaag 
     1201 gtgcgcatgg agggctccat gaacggccac gaattcgaga tcgagggcga gggcgagggc 
     1261 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc 
     1321 ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac 
     1381 cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc 
     1441 gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac 
     1501 ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta 
     1561 atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc 
     1621 gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg 
     1681 gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg 
     1741 gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag 
     1801 cgctccgagg gccgccacca cctgttcctg tacggcatgg acgagctgta caagtaggcg 
     1861 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa 
     1921 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa 
     1981 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 
     2041 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 
     2101 aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca 
     2161 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga 
     2221 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 
     2281 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 
     2341 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag 
     2401 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga 
     2461 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa 
     2521 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg 
     2581 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc 
     2641 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg 
     2701 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc 
     2761 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 
     2821 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 
     2881 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 
     2941 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 
     3001 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc 
     3061 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc 
     3121 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct 
     3181 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg 
     3241 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca 
     3301 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc 
     3361 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga 
     3421 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc 
     3481 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc 
     3541 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg 
     3601 ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct 
     3661 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc 
     3721 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc 
     3781 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc 
     3841 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga 
     3901 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt 
     3961 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg 
     4021 cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa 
     4081 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca 
     4141 cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga 
     4201 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca 
     4261 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc 
     4321 catagcctca ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa 
     4381 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 
     4441 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 
     4501 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 
     4561 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 
     4621 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt 
     4681 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 
     4741 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 
     4801 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 
     4861 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 
     4921 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 
     4981 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 
     5041 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 
     5101 acggttcctg gccttttgct ggccttttgc tcacatgtag agaaatgttc tggcacctgc 
     5161 acttgcactg gggacagcct attttgctag tttgttttgt ttcgttttgt tttgatggag 
     5221 agcgtatgtt agtactatcg attcacacaa aaaaccaaca cacagatgta atgaaaataa 
     5281 agatatttta ttacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta 
     5341 ccgccatgca t 
//

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