pCMV-VSV-G

价格:1250元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Addgene
试剂名称: pCMV-VSV-G
质粒类型: 哺乳动物载体;逆病毒包装;双质粒包装系统;信封载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 6507 bp
5' 测序引物及序列: CMV fwd 5’CGCAAATGGGCGGTAGGCGTG 3’
3' 测序引物及序列: --
载体标签:
载体抗性: Ampicillin
筛选标记:
克隆菌株: DH5α等
宿主细胞(系): 包装细胞系如293T
备注: 逆病毒包装载体pCMV-VSV-G需要与pUMVC共同使用,使用方法见下文。
产品目录号: #8454
稳定性: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pCMV-VSV-G载体图谱



载体简介

双质粒系统逆病毒包装质粒pCMV-VSV-G使用方法

Day 1

1.      Plate 5 x 105 293T cells in 6 cm2 dishes containing 5 mL of media. (This can be scaled up if desired)


Day 2

1.      Set up (use polypropylene tubes for this; polystyrene tubes DO NOT work!):

           1 µg retroviral DNA encoding gene X
           1 µg packaging plasmid Mix
                the packaging plasmid (pUMVC3) at a 8:1 ratio with the envelope plasmid (pCVM-VSV-G)-a total of 1µg
           DME without serum to 94μL total
           6 μL Fugene

       Mix and wait 15 to 30 minutes at room temperature

       Add to 293T cells without touching the sides of the dish (DO NOT CHANGE MEDIA)

       If you are using amphotropic virus then move immediately to BL2+ in a secondary container, which has an absorbent material. 

       (This does not mean a couple of hours; it means Immediately!).  The rest of this protocol is the same for all viruses---the BL2+ safety practices are in place if you are using amphotropic viruses 


Day 3

1.      Change the media to whatever media you wish to use when infecting target cells. 293T cells are easily detached so remember not to put the media directly onto to cells, but rather “run” it down the side of the dish. Remember that you will get the highest titer virus when your cells are “happy.”

2.      Plate out your target cells


Day 4

1.      Remove the medium from the 293T cells and use a 0.45 u syringe filter to remove any 293T cells. DO NOT use the 0.2 u filter, as it is likely to shear the envelope from your virus
making it noninfectious

        Note: After filtering, the filter should be removed and placed in the biohazard bag in the hood and the syringe rinsed with bleach and decontaminated for a minimum of 20 min. It is useful to place a plastic beaker with bleach in the hood in advance

2.      Add 8 to 10 μg/ml of polybrene (Hexadimethrine bromide) or protamine sulfate to the target cells

3.      Carry out infection for 1 to 4 hours. Remove virus and replace with fresh media

        Note: If you wish to do a second infection the following day, it is important to put fresh media on the cells and not let the virus remain on the cells overnight. The media contains huge amounts of envelope, both associated and unassociated with viral particles, which will bind all the cell surface receptors required for virus adsorption, resulting in their down-regulation. Hence, if you don’t change the media after the initial infection, very few receptors will be available for the next round of infection. In addition, very few cells tolerate the presence of high levels of the VSV-G envelope for extended periods of time (i.e. a lot of your cells may die)


Day 5 +

1.      Allow the cells to recover and begin to express the virus-encoded genes. The cells usually require 48 hours for this to occur

2.      Add drug if you are scoring for the presence of a vector that carries a drug resistance marker. Prior to this step it is advisable that you titrate the drug to be used for selection in order to know precisely how much to add. In addition, it is necessary to bring an extra plate of uninfected cells (often referred to as “canaries”) which will function as a positive control in the kill assay.  Add drug to both plates. When your canaries are dead, you can remove the drug.

载体序列

Please note that depositor's sequence is not 100% complete. The flanking sequence of VSV-G actually contains XhoI (at both the 5' and 3'end).

LOCUS       pCMV-VSV-G Sequencing Result	6507 bp 	DNA	SYN
DEFINITION  pCMV-VSV-G Sequencing Result
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6507
                     /organism="pCMV-VSV-G Sequencing Result"
                     /mol_type="other DNA"
     promoter        84..660
                     /label="CMV_immearly_promoter"
     misc_feature    148..974
                     /label="5_LTR"
     misc_feature    163..450
                     /label="CAG_enhancer"
     misc_feature    617..637
                     /label="CMV_fwd_primer"
     promoter        618..687
                     /label="CMV_promoter"
     promoter        630..749
                     /label="CMV2_promoter"
     misc_feature    661..680
                     /label="pCEP_fwd_primer"
     misc_feature    663..687
                     /label="LNCX_primer"
     misc_feature    778..1350
                     /label="bGlob_int"
     CDS             1322..2971
                     /label="ORF frame 2"
                     /translation="MFMPSSLSYSSWATCWLLCCLIILAKNSSTDPSRNSDTMKCLLY
                     LAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSDLNWHNDLIGTALQVKMPKS
                     HKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPSVEQCKESIEQTKQGTWLN
                     PGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQFINGKCSNYICPTVHNS
                     TTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFRSNYFAYETGGKACKMQ
                     YCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQTSVDVSLIQDVERILD
                     YSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLKYFETRYIRVDIAAP
                     ILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFPLYMIGHGMLDSDL
                     HLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWFSSWKSSIASFFF
                     IIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK*"
     gene            1436..2959
                     /label="vsv-G"
                     /gene="vsv-G"
                     /translation="MFMPSSLSYSSWATCWLLCCLIILAKNSSTDPSRNSDTMKCLLY
                     LAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSDLNWHNDLIGTALQVKMPKS
                     HKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPSVEQCKESIEQTKQGTWLN
                     PGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQFINGKCSNYICPTVHNS
                     TTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFRSNYFAYETGGKACKMQ
                     YCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQTSVDVSLIQDVERILD
                     YSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLKYFETRYIRVDIAAP
                     ILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFPLYMIGHGMLDSDL
                     HLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWFSSWKSSIASFFF
                     IIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK*"
     misc_feature    2936..2968
                     /label="VSVG Tag"
                     /translation="MFMPSSLSYSSWATCWLLCCLIILAKNSSTDPSRNSDTMKCLLY
                     LAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSDLNWHNDLIGTALQVKMPKS
                     HKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPSVEQCKESIEQTKQGTWLN
                     PGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQFINGKCSNYICPTVHNS
                     TTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFRSNYFAYETGGKACKMQ
                     YCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQTSVDVSLIQDVERILD
                     YSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLKYFETRYIRVDIAAP
                     ILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFPLYMIGHGMLDSDL
                     HLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWFSSWKSSIASFFF
                     IIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK*"
     terminator      3118..3644
                     /label="rb_glob_PA_terminator"
                     /translation="MFMPSSLSYSSWATCWLLCCLIILAKNSSTDPSRNSDTMKCLLY
                     LAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSDLNWHNDLIGTALQVKMPKS
                     HKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPSVEQCKESIEQTKQGTWLN
                     PGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQFINGKCSNYICPTVHNS
                     TTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFRSNYFAYETGGKACKMQ
                     YCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQTSVDVSLIQDVERILD
                     YSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLKYFETRYIRVDIAAP
                     ILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFPLYMIGHGMLDSDL
                     HLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWFSSWKSSIASFFF
                     IIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK*"
     misc_feature    complement(3689..3711)
                     /label="M13_pUC_rev_primer"
                     /translation="MFMPSSLSYSSWATCWLLCCLIILAKNSSTDPSRNSDTMKCLLY
                     LAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSDLNWHNDLIGTALQVKMPKS
                     HKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPSVEQCKESIEQTKQGTWLN
                     PGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQFINGKCSNYICPTVHNS
                     TTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFRSNYFAYETGGKACKMQ
                     YCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQTSVDVSLIQDVERILD
                     YSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLKYFETRYIRVDIAAP
                     ILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFPLYMIGHGMLDSDL
                     HLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWFSSWKSSIASFFF
                     IIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK*"
     promoter        complement(3725..3754)
                     /label="lac_promoter"
                     /translation="MFMPSSLSYSSWATCWLLCCLIILAKNSSTDPSRNSDTMKCLLY
                     LAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSDLNWHNDLIGTALQVKMPKS
                     HKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPSVEQCKESIEQTKQGTWLN
                     PGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQFINGKCSNYICPTVHNS
                     TTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFRSNYFAYETGGKACKMQ
                     YCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQTSVDVSLIQDVERILD
                     YSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLKYFETRYIRVDIAAP
                     ILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFPLYMIGHGMLDSDL
                     HLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWFSSWKSSIASFFF
                     IIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK*"
     gene            complement(4837..5697)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MFMPSSLSYSSWATCWLLCCLIILAKNSSTDPSRNSDTMKCLLY
                     LAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSDLNWHNDLIGTALQVKMPKS
                     HKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPSVEQCKESIEQTKQGTWLN
                     PGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQFINGKCSNYICPTVHNS
                     TTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFRSNYFAYETGGKACKMQ
                     YCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQTSVDVSLIQDVERILD
                     YSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLKYFETRYIRVDIAAP
                     ILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFPLYMIGHGMLDSDL
                     HLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWFSSWKSSIASFFF
                     IIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK*"
     CDS             complement(4837..5697)
                     /label="ORF frame 1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(5739..5767)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    complement(6284..6439)
                     /label="lacZ_a"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    6410..6432
                     /label="M13_pUC_fwd_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        6425..6441
                     /label="M13_forward20_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        6451..6469
                     /label="T7_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 gagcttggcc cattgcatac gttgtatcca tatcataata tgtacattta tattggctca
   61 tgtccaacat taccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt
  121 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
  181 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
  241 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
  301 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
  361 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
  421 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
  481 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
  541 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
  601 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
  661 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
  721 catagaagac accgggaccg atccagcctc cggtcgaccg atcctgagaa cttcagggtg
  781 agtttgggga cccttgattg ttctttcttt ttcgctattg taaaattcat gttatatgga
  841 gggggcaaag ttttcagggt gttgtttaga atgggaagat gtcccttgta tcaccatgga
  901 ccctcatgat aattttgttt ctttcacttt ctactctgtt gacaaccatt gtctcctctt
  961 attttctttt cattttctgt aactttttcg ttaaacttta gcttgcattt gtaacgaatt
 1021 tttaaattca cttttgttta tttgtcagat tgtaagtact ttctctaatc actttttttt
 1081 caaggcaatc agggtatatt atattgtact tcagcacagt tttagagaac aattgttata
 1141 attaaatgat aaggtagaat atttctgcat ataaattctg gctggcgtgg aaatattctt
 1201 attggtagaa acaactacac cctggtcatc atcctgcctt tctctttatg gttacaatga
 1261 tatacactgt ttgagatgag gataaaatac tctgagtcca aaccgggccc ctctgctaac
 1321 catgttcatg ccttcttctc tttcctacag ctcctgggca acgtgctggt tgttgtgctg
 1381 tctcatcatt ttggcaaaga attcctcgac ggatccctcg aggaattctg acactatgaa
 1441 gtgccttttg tacttagcct ttttattcat tggggtgaat tgcaagttca ccatagtttt
 1501 tccacacaac caaaaaggaa actggaaaaa tgttccttct aattaccatt attgcccgtc
 1561 aagctcagat ttaaattggc ataatgactt aataggcaca gccttacaag tcaaaatgcc
 1621 caagagtcac aaggctattc aagcagacgg ttggatgtgt catgcttcca aatgggtcac
 1681 tacttgtgat ttccgctggt atggaccgaa gtatataaca cattccatcc gatccttcac
 1741 tccatctgta gaacaatgca aggaaagcat tgaacaaacg aaacaaggaa cttggctgaa
 1801 tccaggcttc cctcctcaaa gttgtggata tgcaactgtg acggatgccg aagcagtgat
 1861 tgtccaggtg actcctcacc atgtgctggt tgatgaatac acaggagaat gggttgattc
 1921 acagttcatc aacggaaaat gcagcaatta catatgcccc actgtccata actctacaac
 1981 ctggcattct gactataagg tcaaagggct atgtgattct aacctcattt ccatggacat
 2041 caccttcttc tcagaggacg gagagctatc atccctggga aaggagggca cagggttcag
 2101 aagtaactac tttgcttatg aaactggagg caaggcctgc aaaatgcaat actgcaagca
 2161 ttggggagtc agactcccat caggtgtctg gttcgagatg gctgataagg atctctttgc
 2221 tgcagccaga ttccctgaat gcccagaagg gtcaagtatc tctgctccat ctcagacctc
 2281 agtggatgta agtctaattc aggacgttga gaggatcttg gattattccc tctgccaaga
 2341 aacctggagc aaaatcagag cgggtcttcc aatctctcca gtggatctca gctatcttgc
 2401 tcctaaaaac ccaggaaccg gtcctgcttt caccataatc aatggtaccc taaaatactt
 2461 tgagaccaga tacatcagag tcgatattgc tgctccaatc ctctcaagaa tggtcggaat
 2521 gatcagtgga actaccacag aaagggaact gtgggatgac tgggcaccat atgaagacgt
 2581 ggaaattgga cccaatggag ttctgaggac cagttcagga tataagtttc ctttatacat
 2641 gattggacat ggtatgttgg actccgatct tcatcttagc tcaaaggctc aggtgttcga
 2701 acatcctcac attcaagacg ctgcttcgca acttcctgat gatgagagtt tattttttgg
 2761 tgatactggg ctatccaaaa atccaatcga gcttgtagaa ggttggttca gtagttggaa
 2821 aagctctatt gcctcttttt tctttatcat agggttaatc attggactat tcttggttct
 2881 ccgagttggt atccatcttt gcattaaatt aaagcacacc aagaaaagac agatttatac
 2941 agacatagag atgaaccgac ttggaaagta actcaaatcc tgcacaacag attcttcatg
 3001 tttggaccaa atcaacttgt gataccatgc tcaaagaggc ctcaattata tttgagtttt
 3061 taatttttat gaaaaaaaaa aaaaaaaacg gaattcctcg agggatccgt cgaggaattc
 3121 actcctcagg tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct
 3181 cacaaatacc actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc
 3241 cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg
 3301 aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag
 3361 aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa
 3421 ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc
 3481 catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt
 3541 tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc
 3601 tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga cggatcggcc
 3661 gcaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
 3721 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
 3781 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
 3841 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
 3901 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
 3961 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
 4021 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
 4081 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
 4141 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
 4201 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
 4261 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
 4321 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
 4381 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
 4441 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
 4501 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
 4561 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
 4621 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
 4681 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
 4741 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
 4801 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
 4861 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
 4921 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
 4981 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
 5041 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
 5101 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
 5161 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
 5221 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
 5281 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
 5341 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
 5401 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
 5461 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
 5521 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
 5581 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
 5641 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
 5701 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
 5761 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
 5821 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
 5881 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
 5941 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
 6001 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
 6061 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
 6121 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
 6181 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
 6241 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg
 6301 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
 6361 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
 6421 cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg cgaattggag
 6481 ctccaccgcg gtggcggccg ctctaga
//

载体质粒图谱pdf版和相关资料下载

载体应用举例

分享到:
Copyright © 20012-2013 BIOFENG. 生物风 版权所有 Powered by Biofeng