pBABE-hygro-hTERT

价格:1500元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品人: Bob Weinberg
载体名称: pBABE-hygro-hTERT
质粒类型: 逆病毒载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: MSCV
载体大小: 8703 bp
5' 测序引物及序列: pBABE 5' primer 5'CTTTATCCAGCCCTCAC3'
3' 测序引物及序列: pBABE 3' primer 5'ACCCTAACTGACACACATTCC3'
载体标签:
载体抗性: 氨苄青霉素
筛选标记: Hygromycin
克隆菌株: DH5α、HB101等
宿主细胞(系): 常规哺乳动物细胞系
备注:
逆病毒载体pBABE-hygro-hTERT表达telomerase reverse transcriptase,用来建永生细胞系。
推荐使用逆病毒包装载体:pUMVCpCMV-VSV-G
 
产品目录号: #1773
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 逆转录病毒

载体质粒图谱和多克隆位点信息

pBABE-hygro-hTERT载体图谱



载体简介

载体序列

LOCUS       pBABE-hygro-hTERT Sequencing Result	8703 bp 	DNA	SYN
DEFINITION  pBABE-hygro-hTERT Sequencing Result
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..8703
                     /organism="pBABE-hygro-hTERT Sequencing Result"
                     /mol_type="other DNA"
     misc_feature    10..477
                     /label="5_LTR2"
     misc_feature    16..477
                     /label="3MoMuLV_LTR"
     misc_feature    547..1356
                     /label="psi_plus_pack"
     misc_feature    943..1350
                     /label="gag"
     misc_feature    1286..1308
                     /label="MSCV_primer"
     misc_feature    1286..1308
                     /label="pLXSN_5_primer"
     misc_feature    1324..1340
                     /label="pBABE_5_primer"
     CDS             complement(1337..2050)
                     /label="ORF frame 3"
                     /translation="MVPGPFASQTPSGSTSVWRGPGLSGSAELVQRRPAHLVGAAGSH
                     QHKERAACQQVNQHVVAHAAQQQPPRPAPPQCVGHRVGQVAAHAGGEGLGGAPAGPVQ
                     QREAEGQHVLRAALAQPLQHSGHQLLQAGHLAEGGGGGGRPCVPGHAHQALGHQRAES
                     RRVPALHQPPALGPQAPHERGQRQHLAVVAAQQGAHGSAAGSARHRGGGRGQGFPRAQ
                     QDEFLRTTTLGSGRRLEKE*"
     CDS             1436..4834
                     /label="ORF frame 2"
                     /translation="MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDP
                     AAFRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFA
                     LLDGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFV
                     LVAPSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPA
                     PGARRRGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPA
                     RPAEEATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYS
                     SGDKEQLRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPL
                     FLELLGNHAQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQ
                     LLRQHSSPWQVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQEL
                     TWKMSVRGCAWLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTET
                     TFQKNRLFFYRKSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFI
                     PKPDGLRPIVNMDYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLG
                     LDDIHRAWRTFVLRVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYC
                     VRRYAVVQKAAHGHVRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSL
                     NEASSGLFDVFLRFMCHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAG
                     IRRDGLLLRLVDDFLLVTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEAL
                     GGTAFVQMPAHGLFPWCGLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRR
                     KLFGVLRLKCHSLFLDLQVNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPT
                     FFLRVISDTASLCYSILKAKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVT
                     YVPLLGSLRTAQTQLSRKLPGTTLTALEAAANPALPSDFKTILD*"
     CDS             complement(2471..3691)
                     /label="ORF frame 3"
                     /translation="MGGLLDHGIPTHAVRVLGFDDAGDDLREPVLGDGVVRARHIHLD
                     KVQLRRRVLGPHTQHEGAPGPVDIVQAQHRGAQEAGAPRPLVVEHAEQCLHPRGETLG
                     PLFSAERSGSHDVVHVHNRPQPVRLGDEAESGRQQGGPGFPMLPDLCFRQLPQLHPLQ
                     VLSDSNALQLAPDTLPVEKEPVLLKRGLRDIKERPEQLDDVHTHQPVQELGQDLLTQT
                     VLCGRNTANPWAPAQPSAAPHAHLPRQLLQRELGMLPQGDELLGVPEEAAFVVPGAPE
                     AWGHQPAQAGPHEAVHLPGAAVLAEQLHQATGVCVLLLGGRHRALGLLPGTDTGCWGD
                     RSSQRAVRLEEHPVGALRVVPKQLQKQGPHLPVALGQAGQPAGSPWHPGPGTQKDGLH
                     EPPSASQAGPQRAE*"
     misc_feature    complement(4846..4866)
                     /label="pBABE_3_primer"
                     /translation="MGGLLDHGIPTHAVRVLGFDDAGDDLREPVLGDGVVRARHIHLD
                     KVQLRRRVLGPHTQHEGAPGPVDIVQAQHRGAQEAGAPRPLVVEHAEQCLHPRGETLG
                     PLFSAERSGSHDVVHVHNRPQPVRLGDEAESGRQQGGPGFPMLPDLCFRQLPQLHPLQ
                     VLSDSNALQLAPDTLPVEKEPVLLKRGLRDIKERPEQLDDVHTHQPVQELGQDLLTQT
                     VLCGRNTANPWAPAQPSAAPHAHLPRQLLQRELGMLPQGDELLGVPEEAAFVVPGAPE
                     AWGHQPAQAGPHEAVHLPGAAVLAEQLHQATGVCVLLLGGRHRALGLLPGTDTGCWGD
                     RSSQRAVRLEEHPVGALRVVPKQLQKQGPHLPVALGQAGQPAGSPWHPGPGTQKDGLH
                     EPPSASQAGPQRAE*"
     misc_feature    complement(4852..5231)
                     /label="SV40_enhancer"
                     /translation="MGGLLDHGIPTHAVRVLGFDDAGDDLREPVLGDGVVRARHIHLD
                     KVQLRRRVLGPHTQHEGAPGPVDIVQAQHRGAQEAGAPRPLVVEHAEQCLHPRGETLG
                     PLFSAERSGSHDVVHVHNRPQPVRLGDEAESGRQQGGPGFPMLPDLCFRQLPQLHPLQ
                     VLSDSNALQLAPDTLPVEKEPVLLKRGLRDIKERPEQLDDVHTHQPVQELGQDLLTQT
                     VLCGRNTANPWAPAQPSAAPHAHLPRQLLQRELGMLPQGDELLGVPEEAAFVVPGAPE
                     AWGHQPAQAGPHEAVHLPGAAVLAEQLHQATGVCVLLLGGRHRALGLLPGTDTGCWGD
                     RSSQRAVRLEEHPVGALRVVPKQLQKQGPHLPVALGQAGQPAGSPWHPGPGTQKDGLH
                     EPPSASQAGPQRAE*"
     promoter        4864..5132
                     /label="SV40_promoter"
                     /translation="MGGLLDHGIPTHAVRVLGFDDAGDDLREPVLGDGVVRARHIHLD
                     KVQLRRRVLGPHTQHEGAPGPVDIVQAQHRGAQEAGAPRPLVVEHAEQCLHPRGETLG
                     PLFSAERSGSHDVVHVHNRPQPVRLGDEAESGRQQGGPGFPMLPDLCFRQLPQLHPLQ
                     VLSDSNALQLAPDTLPVEKEPVLLKRGLRDIKERPEQLDDVHTHQPVQELGQDLLTQT
                     VLCGRNTANPWAPAQPSAAPHAHLPRQLLQRELGMLPQGDELLGVPEEAAFVVPGAPE
                     AWGHQPAQAGPHEAVHLPGAAVLAEQLHQATGVCVLLLGGRHRALGLLPGTDTGCWGD
                     RSSQRAVRLEEHPVGALRVVPKQLQKQGPHLPVALGQAGQPAGSPWHPGPGTQKDGLH
                     EPPSASQAGPQRAE*"
     rep_origin      5031..5108
                     /label="SV40_origin"
                     /translation="MGGLLDHGIPTHAVRVLGFDDAGDDLREPVLGDGVVRARHIHLD
                     KVQLRRRVLGPHTQHEGAPGPVDIVQAQHRGAQEAGAPRPLVVEHAEQCLHPRGETLG
                     PLFSAERSGSHDVVHVHNRPQPVRLGDEAESGRQQGGPGFPMLPDLCFRQLPQLHPLQ
                     VLSDSNALQLAPDTLPVEKEPVLLKRGLRDIKERPEQLDDVHTHQPVQELGQDLLTQT
                     VLCGRNTANPWAPAQPSAAPHAHLPRQLLQRELGMLPQGDELLGVPEEAAFVVPGAPE
                     AWGHQPAQAGPHEAVHLPGAAVLAEQLHQATGVCVLLLGGRHRALGLLPGTDTGCWGD
                     RSSQRAVRLEEHPVGALRVVPKQLQKQGPHLPVALGQAGQPAGSPWHPGPGTQKDGLH
                     EPPSASQAGPQRAE*"
     CDS             complement(5092..6012)
                     /label="ORF frame 1"
                     /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
                     AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
                     YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
                     AGDAIGQALAKFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
                     TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPPGIRSAGLPNLTRGRPSWQ
                     AFCKSLGLQKSLLTTSGIAQRPRRPRPLHK*"
     misc_feature    5093..5112
                     /label="SV40pro_F_primer"
                     /translation="MPPLEVARLLLHTSQPRPPEEDVGDLVLGIPEHRLAPVNDRCYA
                     AIVRQDIVGAEIRVHEVPDFGAVLGPKHQLIESLRDGRTDGVVHHSLPVIHMGISNRA
                     YEITPCSVLTDSLRSEWAEPARLAKIGRSDRIHGLRDRLQNSGQFGFRQVLQRDTLCT
                     AGDAIGQALAKFPNVKHFRNRERGRCKVPINITIFVETIGAAIYPQDISTPSYIEAES
                     TRFFALRELHQVGDAVELFDQKLLDRRRGEFRLFHISLPPGIRSAGLPNLTRGRPSWQ
                     AFCKSLGLQKSLLTTSGIAQRPRRPRPLHK*"
     CDS             5254..6279
                     /label="ORF frame 1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     gene            5269..6276
                     /label="hygroB"
                     /gene="hygroB"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     gene            complement(7661..8521)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE*"
     CDS             complement(7661..8521)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(8563..8591)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    8661..477
                     /label="5_LTR2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    8667..477
                     /label="3MoMuLV_LTR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 gcagcctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccGGCTCAGG
   61 GCCAAGAACA GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC
  121 CTGCCCCGGC TCAGGGCCAA GAACAGATGG TCCCCAGATG CGGTCCAGCC CTCAGCAGTT
  181 TCTAGAGAAC CATCAGATGT TTCCAGGGTG CCCCAAGGAC CTGAAATGAC CCTGTGCCTT
  241 ATTTGAACTA ACCAATCAGT TCGCTTCTCG CTTCTGTTCG CGCGCTTCTG CTCCCCGAGC
  301 TCAATAAAAG AGCCCACAAC CCCTCACTCG GGGCGCCAGT CCTCCGATTG ACTGAGTCGC
  361 CCGGGTACCC GTGTATCCAA TAAACCCTCT TGCAGTTGCA TCCGACTTGT GGTCTCGCTG
  421 TTCCTTGGGA GGGTCTCCTC TGAGTGATTG ACTACCCGTC AGCGGGGGTC TTTCATTTGG
  481 GGGCTCGTCC GGGATCGGGA GACCCCTGCC CAGGGACCAC CGACCCACCA CCGGGAGGCA
  541 AGCTGGCCAG CAACTTATCT GTGTCTGTCC GATTGTCTAG TGTCTATGAC TGATTTTATG
  601 CGCCTGCGTC GGTACTAGTT AGCTAACTAG CTCTGTATCT GGCGGACCCG TGGTGGAACT
  661 GACGAGTTCT GAACACCCGG CCGCAACCCT GGGAGACGTC CCAGGGACTT TGGGGGCCGT
  721 TTTTGTGGCC CGACCTGAGG AAGGGAGTCG ATGTGGAATC CGACCCCGTC AGGATATGTG
  781 GTTCTGGTAG GAGACGAGAA CCTAAAACAG TTCCCGCCTC CGTCTGAATT TTTGCTTTCG
  841 GTTTGGAACC GAAGCCGCGC GTCTTGTCTG CTGCAGCGCT GCAGCATCGT TCTGTGTTGT
  901 CTCTGTCTGA CTGTGTTTCT GTATTTGTCT GAAAATTAGG GCCAGACTGT TACCACTCCC
  961 TTAAGTTTGA CCTTAGGTCA CTGGAAAGAT GTCGAGCGGA TCGCTCACAA CCAGTCGGTA
 1021 GATGTCAAGA AGAGACGTTG GGTTACCTTC TGCTCTGCAG AATGGCCAAC CTTTAACGTC
 1081 GGATGGCCGC GAGACGGCAC CTTTAACCGA GACCTCATCA CCCAGGTTAA GATCAAGGTC
 1141 TTTTCACCTG GCCCGCATGG ACACCCAGAC CAGGTCCCCT ACATCGTGAC CTGGGAAGCC
 1201 TTGGCTTTTG ACCCCCCTCC CTGGGTCAAG CCCTTTGTAC ACCCTAAGCC TCCGCCTCCT
 1261 CTTCCTCCAT CCGCCCCGTC TCTCCCCCTT GAACCTCCTC GTTCGACCCC GCCTCGATCC
 1321 TCCCTTTATC CAGCCCTCAC TCCTTCTCTA GGCGCCGGCC GGATCCCAGT GTGGTGGTAC
 1381 GTAGGAATTC GTCCTGCTGC GCACGTGGGA AGCCCTGGCC CCGGCCACCC CCGCGATGCC
 1441 GCGCGCTCCC CGCTGCCGAG CCGTGCGCTC CCTGCTGCGC AGCCACTACC GCGAGGTGCT
 1501 GCCGCTGGCC ACGTTCGTGC GGCGCCTGGG GCCCCAGGGC TGGCGGCTGG TGCAGCGCGG
 1561 GGACCCGGCG GCTTTCCGCG CGCTGGTGGC CCAGTGCCTG GTGTGCGTGC CCTGGGACGC
 1621 ACGGCCGCCC CCCGCCGCCC CCTCCTTCCG CCAGGTGTCC TGCCTGAAGG AGCTGGTGGC
 1681 CCGAGTGCTG CAGAGGCTGT GCGAGCGCGG CGCGAAGAAC GTGCTGGCCT TCGGCTTCGC
 1741 GCTGCTGGAC GGGGCCCGCG GGGGCCCCCC CGAGGCCTTC ACCACCAGCG TGCGCAGCTA
 1801 CCTGCCCAAC ACGGTGACCG ACGCACTGCG GGGGAGCGGG GCGTGGGGGC TGCTGCTGCG
 1861 CCGCGTGGGC GACGACGTGC TGGTTCACCT GCTGGCACGC TGCGCGCTCT TTGTGCTGGT
 1921 GGCTCCCAGC TGCGCCTACC AGGTGTGCGG GCCGCCGCTG TACCAGCTCG GCGCTGCCAC
 1981 TCAGGCCCGG CCCCCGCCAC ACGCTAGTGG ACCCCGAAGG CGTCTGGGAT GCGAACGGGC
 2041 CTGGAACCAT AGCGTCAGGG AGGCCGGGGT CCCCCTGGGC CTGCCAGCCC CGGGTGCGAG
 2101 GAGGCGCGGG GGCAGTGCCA GCCGAAGTCT GCCGTTGCCC AAGAGGCCCA GGCGTGGCGC
 2161 TGCCCCTGAG CCGGAGCGGA CGCCCGTTGG GCAGGGGTCC TGGGCCCACC CGGGCAGGAC
 2221 GCGTGGACCG AGTGACCGTG GTTTCTGTGT GGTGTCACCT GCCAGACCCG CCGAAGAAGC
 2281 CACCTCTTTG GAGGGTGCGC TCTCTGGCAC GCGCCACTCC CACCCATCCG TGGGCCGCCA
 2341 GCACCACGCG GGCCCCCCAT CCACATCGCG GCCACCACGT CCCTGGGACA CGCCTTGTCC
 2401 CCCGGTGTAC GCCGAGACCA AGCACTTCCT CTACTCCTCA GGCGACAAGG AGCAGCTGCG
 2461 GCCCTCCTTC CTACTCAGCT CTCTGAGGCC CAGCCTGACT GGCGCTCGGA GGCTCGTGGA
 2521 GACCATCTTT CTGGGTTCCA GGCCCTGGAT GCCAGGGACT CCCCGCAGGT TGCCCCGCCT
 2581 GCCCCAGCGC TACTGGCAAA TGCGGCCCCT GTTTCTGGAG CTGCTTGGGA ACCACGCGCA
 2641 GTGCCCCTAC GGGGTGCTCC TCAAGACGCA CTGCCCGCTG CGAGCTGCGG TCACCCCAGC
 2701 AGCCGGTGTC TGTGCCCGGG AGAAGCCCCA GGGCTCTGTG GCGGCCCCCG AGGAGGAGGA
 2761 CACAGACCCC CGTCGCCTGG TGCAGCTGCT CCGCCAGCAC AGCAGCCCCT GGCAGGTGTA
 2821 CGGCTTCGTG CGGGCCTGCC TGCGCCGGCT GGTGCCCCCA GGCCTCTGGG GCTCCAGGCA
 2881 CAACGAACGC CGCTTCCTCA GGAACACCAA GAAGTTCATC TCCCTGGGGA AGCATGCCAA
 2941 GCTCTCGCTG CAGGAGCTGA CGTGGAAGAT GAGCGTGCGG GGCTGCGCTT GGCTGCGCAG
 3001 GAGCCCAGGG GTTGGCTGTG TTCCGGCCGC AGAGCACCGT CTGCGTGAGG AGATCCTGGC
 3061 CAAGTTCCTG CACTGGCTGA TGAGTGTGTA CGTCGTCGAG CTGCTCAGGT CTTTCTTTTA
 3121 TGTCACGGAG ACCACGTTTC AAAAGAACAG GCTCTTTTTC TACCGGAAGA GTGTCTGGAG
 3181 CAAGTTGCAA AGCATTGGAA TCAGACAGCA CTTGAAGAGG GTGCAGCTGC GGGAGCTGTC
 3241 GGAAGCAGAG GTCAGGCAGC ATCGGGAAGC CAGGCCCGCC CTGCTGACGT CCAGACTCCG
 3301 CTTCATCCCC AAGCCTGACG GGCTGCGGCC GATTGTGAAC ATGGACTACG TCGTGGGAGC
 3361 CAGAACGTTC CGCAGAGAAA AGAGGGCCGA GCGTCTCACC TCGAGGGTGA AGGCACTGTT
 3421 CAGCGTGCTC AACTACGAGC GGGCGCGGCG CCCCGGCCTC CTGGGCGCCT CTGTGCTGGG
 3481 CCTGGACGAT ATCCACAGGG CCTGGCGCAC CTTCGTGCTG CGTGTGCGGG CCCAGGACCC
 3541 GCCGCCTGAG CTGTACTTTG TCAAGGTGGA TGTGACGGGC GCGTACGACA CCATCCCCCA
 3601 GGACAGGCTC ACGGAGGTCA TCGCCAGCAT CATCAAACCC CAGAACACGT ACTGCGTGCG
 3661 TCGGTATGCC GTGGTCCAGA AGGCCGCCCA TGGGCACGTC CGCAAGGCCT TCAAGAGCCA
 3721 CGTCTCTACC TTGACAGACC TCCAGCCGTA CATGCGACAG TTCGTGGCTC ACCTGCAGGA
 3781 GACCAGCCCG CTGAGGGATG CCGTCGTCAT CGAGCAGAGC TCCTCCCTGA ATGAGGCCAG
 3841 CAGTGGCCTC TTCGACGTCT TCCTACGCTT CATGTGCCAC CACGCCGTGC GCATCAGGGG
 3901 CAAGTCCTAC GTCCAGTGCC AGGGGATCCC GCAGGGCTCC ATCCTCTCCA CGCTGCTCTG
 3961 CAGCCTGTGC TACGGCGACA TGGAGAACAA GCTGTTTGCG GGGATTCGGC GGGACGGGCT
 4021 GCTCCTGCGT TTGGTGGATG ATTTCTTGTT GGTGACACCT CACCTCACCC ACGCGAAAAC
 4081 CTTCCTCAGG ACCCTGGTCC GAGGTGTCCC TGAGTATGGC TGCGTGGTGA ACTTGCGGAA
 4141 GACAGTGGTG AACTTCCCTG TAGAAGACGA GGCCCTGGGT GGCACGGCTT TTGTTCAGAT
 4201 GCCGGCCCAC GGCCTATTCC CCTGGTGCGG CCTGCTGCTG GATACCCGGA CCCTGGAGGT
 4261 GCAGAGCGAC TACTCCAGCT ATGCCCGGAC CTCCATCAGA GCCAGTCTCA CCTTCAACCG
 4321 CGGCTTCAAG GCTGGGAGGA ACATGCGTCG CAAACTCTTT GGGGTCTTGC GGCTGAAGTG
 4381 TCACAGCCTG TTTCTGGATT TGCAGGTGAA CAGCCTCCAG ACGGTGTGCA CCAACATCTA
 4441 CAAGATCCTC CTGCTGCAGG CGTACAGGTT TCACGCATGT GTGCTGCAGC TCCCATTTCA
 4501 TCAGCAAGTT TGGAAGAACC CCACATTTTT CCTGCGCGTC ATCTCTGACA CGGCCTCCCT
 4561 CTGCTACTCC ATCCTGAAAG CCAAGAACGC AGGGATGTCG CTGGGGGCCA AGGGCGCCGC
 4621 CGGCCCTCTG CCCTCCGAGG CCGTGCAGTG GCTGTGCCAC CAAGCATTCC TGCTCAAGCT
 4681 GACTCGACAC CGTGTCACCT ACGTGCCACT CCTGGGGTCA CTCAGGACAG CCCAGACGCA
 4741 GCTGAGTCGG AAGCTCCCGG GGACGACGCT GACTGCCCTG GAGGCCGCAG CCAACCCGGC
 4801 ACTGCCCTCA GACTTCAAGA CCATCCTGGA CTGAGTCGAC CCTGTGGAAT GTGTGTCAGT
 4861 TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA
 4921 ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA
 4981 GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC
 5041 TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG
 5101 CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG
 5161 GAGGCCTAGG CTTTTGCAAA AAGCTTGCCA GCTGGGGCGC CCTCTGGTAA GGTTGGGAAG
 5221 CCCTGCAGAT CTGATCCCGG GGGGCAATGA GATATGAAAA AGCCTGAACT CACCGCGACG
 5281 TCTGTCGAGA AGTTTCTGAT CGAAAAGTTC GACAGCGTCT CCGACCTGAT GCAGCTCTCG
 5341 GAGGGCGAAG AATCTCGTGC TTTCAGCTTC GATGTAGGAG GGCGTGGATA TGTCCTGCGG
 5401 GTAAATAGCT GCGCCGATGG TTTCTACAAA GATCGTTATG TTTATCGGCA CTTTGCATCG
 5461 GCCGCGCTCC CGATTCCGGA AGTGCTTGAC ATTGGGGAAT TTAGCGAGAG CCTGACCTAT
 5521 TGCATCTCCC GCCGTGCACA GGGTGTCACG TTGCAAGACC TGCCTGAAAC CGAACTGCCC
 5581 GCTGTTCTGC AGCCGGTCGC GGAGGCCATG GATGCGATCG CTGCGGCCGA TCTTAGCCAG
 5641 ACGAGCGGGT TCGGCCCATT CGGACCGCAA GGAATCGGTC AATACACTAC ATGGCGTGAT
 5701 TTCATATGCG CGATTGCTGA TCCCCATGTG TATCACTGGC AAACTGTGAT GGACGACACC
 5761 GTCAGTGCGT CCGTCGCGCA GGCTCTCGAT GAGCTGATGC TTTGGGCCGA GGACTGCCCC
 5821 GAAGTCCGGC ACCTCGTGCA CGCGGATTTC GGCTCCAACA ATGTCCTGAC GGACAATGGC
 5881 CGCATAACAG CGGTCATTGA CTGGAGCGAG GCGATGTTCG GGGATTCCCA ATACGAGGTC
 5941 GCCAACATCT TCTTCTGGAG GCCGTGGTTG GCTTGTATGG AGCAGCAGAC GCGCTACTTC
 6001 GAGCGGAGGC ATCCGGAGCT TGCAGGATCG CCGCGGCTCC GGGCGTATAT GCTCCGCATT
 6061 GGTCTTGACC AACTCTATCA GAGCTTGGTT GACGGCAATT TCGATGATGC AGCTTGGGCG
 6121 CAGGGTCGAT GCGACGCAAT CGTCCGATCC GGAGCCGGGA CTGTCGGGCG TACACAAATC
 6181 GCCCGCAGAA GCGCGGCCGT CTGGACCGAT GGCTGTGTAG AAGTACTCGC CGATAGTGGA
 6241 AACCGACGCC CCAGCACTCG TCCGAGGGCA AAGGAATAGA GTAGATGCCG ACCGGGATCT
 6301 ATCGATGCGG CGGGTGTGGT GGTTACGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC
 6361 GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC
 6421 TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT AGTTAGGCCA
 6481 CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT
 6541 GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC
 6601 GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG
 6661 AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC
 6721 CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC
 6781 GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT
 6841 CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC
 6901 CAGCAACGCA TCGATAAAAT AAAAGATTTT ATTTAGTCTC CAGAAAAAGG GGGGAATGAA
 6961 AGACCCCACC TGTAGGTTTG GCAAGCTAGC TTAAGTAACG CCATTTTGCA AGGCATGGAA
 7021 AAATACATAA CTGAGAATAG AGAAGTTCAG ATCAAGGTCA GGAACAGATG GAACAGCTGA
 7081 ATATGGGCCA AACAGGATAT CTGTGGTAAG CAGTTCCTGC CCCGGCTCAG GGCCAAGAAC
 7141 AGATGGAACA GCTGAATATG GGCCAAACAG GATATCTGTG GTAAGCAGTT CCTGCCCCGG
 7201 CTCAGGGCCA AGAACAGATG GTCCCCAGAT GCGGTCCAGC CCTCAGCAGT TTCTAGAGAA
 7261 CCATCAGATG TTTCCAGGGT GCCCCAAGGA CCTGAAATGA CCCTGTGCCT TATTTGAACT
 7321 AACCAATCAG TTCGCTTCTC GCTTCTGTTC GCGCGCTTCT GCTCCCCGAG CTCAATAAAA
 7381 GAGCCCACAA CCCCTCACTC GGGGCGCCAG TCCTCCGATT GACTGAGTCG CCCGGGTACC
 7441 CGTGTATCCA ATAAACCCTC TTGCAGTTGC ATCCGACTTG TGGTCTCGCT GTTCCTTGGG
 7501 AGGGTCTCCT CTGAGTGATT GACTACCCGT CAGCGGGGGT CTTTCACATG CAGCATGTAT
 7561 CAAAATTAAT TTGGTTTTTT TTCTTAAGTA TTTACATTAA ATGGCCATAG TGCGGCCGCA
 7621 AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG
 7681 AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG
 7741 TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC
 7801 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG
 7861 AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG
 7921 AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG
 7981 GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT
 8041 CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC
 8101 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC
 8161 ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA
 8221 CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC
 8281 GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT
 8341 CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC
 8401 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA
 8461 CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA
 8521 TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT
 8581 ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA
 8641 AAGTGCCACC Tgcagcctga atatgggcca aacaggatat ctgtggtaag cagttcctgc
 8701 ccc
//

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