pLVX-ZsGreen1-C1

价格:1500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pLVX-ZsGreen1-C1
质粒类型: 慢病毒载体;哺乳动物细胞表达载体;荧光报告载体
高拷贝/低拷贝: 高拷贝
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 8763 bp
5' 测序引物及序列: CMV-F: CGCAAATGGGCGGTAGGCGTG (Invitrogen)
3' 测序引物及序列: --
载体标签: ZsGreen1(N-端)
载体抗性: 氨苄青霉素
筛选标记: 嘌呤霉素(Puromycin)
克隆菌株: Stbl3
备注: pLVX-ZsGreen1-C1载体表达的目的蛋白N端融ZsGreen1荧光蛋白;
CMV启动子驱动目的蛋白的过表达;
ZsGreen1是第三代绿色荧光蛋白,亮度极高,可用流式细胞仪筛选。
产品目录号: 632566
稳定性: 稳表达
组成型: 组成型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLVX-ZsGreen1-C1载体图谱



pLVX-ZsGreen1-C1多克隆位点

pLVX-ZsGreen1-C1载体特征

载体简介

载体序列

LOCUS       pLVX-ZsGreen1-C1   8763 bp    DNA   circular             7-OCT-2009
COMMENT     Created by Clontech Laboratories Inc.
COMMENT     Cat. No. 632566
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=packaging\signal
     misc_feature    4731..5322
                     /label=WPRE
     CDS             complement(7449..8445)
                     /label=Amp(R)
     misc_binding    1303..1536
                     /label=RRE
     misc_binding    636..653
                     /label=PBS
     LTR             5525..6161
                     /label=3'\LTR
     promoter        3589..4097
                     /label=PGK\Promoter
     CDS             4118..4717
                     /label=Puro(R)
     misc_feature    3574..3576
                     /label=Stop
     misc_feature    3578..3580
                     /label=Stop
     CDS             2807..3499
                     /label=ZsGreen1
     misc_feature    2028..2151
                     /label=cPPT/CTS\(DNA\flap)
     promoter        2185..2787
                     /label=CMV\IE\Promoter
     rep_origin      complement(6631..7304)
                     /label=pUC\ori
     misc_feature    3582..3584
                     /label=Stop
     misc_feature    3513..3565
                     /label=MCS
BASE COUNT     2162 a      2283 c      2311 g      2007 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 
     2761 tccatagaag acaccgactc tactagagga tctaccggtc gccaccatgg cccagtccaa 
     2821 gcacggcctg accaaggaga tgaccatgaa gtaccgcatg gagggctgcg tggacggcca 
     2881 caagttcgtg atcaccggcg agggcatcgg ctaccccttc aagggcaagc aggccatcaa 
     2941 cctgtgcgtg gtggagggcg gccccttgcc cttcgccgag gacatcttgt ccgccgcctt 
     3001 catgtacggc aaccgcgtgt tcaccgagta cccccaggac atcgtcgact acttcaagaa 
     3061 ctcctgcccc gccggctaca cctgggaccg ctccttcctg ttcgaggacg gcgccgtgtg 
     3121 catctgcaac gccgacatca ccgtgagcgt ggaggagaac tgcatgtacc acgagtccaa 
     3181 gttctacggc gtgaacttcc ccgccgacgg ccccgtgatg aagaagatga ccgacaactg 
     3241 ggagccctcc tgcgagaaga tcatccccgt gcccaagcag ggcatcttga agggcgacgt 
     3301 gagcatgtac ctgctgctga aggacggtgg ccgcttgcgc tgccagttcg acaccgtgta 
     3361 caaggccaag tccgtgcccc gcaagatgcc cgactggcac ttcatccagc acaagctgac 
     3421 ccgcgaggac cgcagcgacg ccaagaacca gaagtggcac ctgaccgagc acgccatcgc 
     3481 ctccggctcc gccttgccct ccggactcag atctcgagct caagcttcga attctgcagt 
     3541 cgacggtacc gcgggcccgg gatccaccgg atctagataa ctgatcataa ttctaccggg 
     3601 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca 
     3661 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc 
     3721 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga 
     3781 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc 
     3841 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg 
     3901 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg 
     3961 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg 
     4021 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct 
     4081 catctccggg cctttcgacc tgcagcccaa gcttaccatg accgagtaca agcccacggt 
     4141 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc 
     4201 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga 
     4261 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga 
     4321 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt 
     4381 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca 
     4441 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt 
     4501 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt 
     4561 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct 
     4621 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc 
     4681 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgaccg cgtctggaac aatcaacctc 
     4741 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc 
     4801 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca 
     4861 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg 
     4921 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca 
     4981 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg 
     5041 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg 
     5101 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg 
     5161 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg 
     5221 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc 
     5281 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg 
     5341 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg 
     5401 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc 
     5461 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag 
     5521 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta 
     5581 ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata 
     5641 tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga 
     5701 ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga 
     5761 cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc 
     5821 ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact 
     5881 ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct 
     5941 cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga 
     6001 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct 
     6061 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat 
     6121 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta 
     6181 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt 
     6241 gctagcgttt taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt 
     6301 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 
     6361 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 
     6421 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 
     6481 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 
     6541 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 
     6601 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 
     6661 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 
     6721 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 
     6781 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 
     6841 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 
     6901 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 
     6961 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 
     7021 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 
     7081 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 
     7141 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 
     7201 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 
     7261 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 
     7321 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 
     7381 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 
     7441 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 
     7501 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 
     7561 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 
     7621 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 
     7681 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 
     7741 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 
     7801 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 
     7861 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 
     7921 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 
     7981 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 
     8041 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 
     8101 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 
     8161 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 
     8221 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 
     8281 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 
     8341 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 
     8401 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat 
     8461 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 
     8521 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 
     8581 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac 
     8641 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc 
     8701 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt 
     8761 agt 
//

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