| 出品公司: | BD Biosciences |
|---|---|
| 载体名称: | pAcHLT B |
| 质粒类型: | 昆虫细胞载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 8110 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
pAcHLT B载体 pAcHLTB质粒基本信息
pAcHLT B质粒图谱 pAcHLTB载体图谱和pAcHLT B载体序列 pAcHLTB质粒序列多克隆位点信息
pAcHLT B质粒 pAcHLTB载体简介
pAcHLT B质粒序列 pAcHLTB载体序列
LOCUS pAcHLT B 8110 bp ds-DNA circular SYN 19-APR-2014
DEFINITION Baculovirus transfer vector for expressing proteins with an
N-terminal 6xHis-PKA-thrombin cassette. For other reading frames,
use pAcHLT A or pAcHLT C.
ACCESSION .
VERSION .
KEYWORDS pAcHLT B
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8110)
AUTHORS BD Biosciences
TITLE Direct Submission
JOURNAL Exported Wednesday, February 5, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8110
/organism="synthetic DNA construct"
/lab_host="Insect Cells"
/mol_type="other DNA"
misc_recomb 1269..2096
/note="baculovirus recombination region (lef2/ORF603)"
/note="contains ORF603 and part of lef2"
CDS 1269..1393
/codon_start=3
/product="baculovirus late expression factor 2"
/note="lef2"
/translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
CDS complement(1431..2036)
/codon_start=1
/product="baculovirus ORF603 protein"
/note="ORF603"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
promoter 2100..2191
/gene="polh from Autographa californica"
/note="polyhedrin promoter"
/note="promoter for the baculovirus polyhedrin gene"
CDS 2230..2247
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
CDS 2254..2271
/codon_start=1
/product="recognition site for phosphorylation by protein
kinase A"
/note="PKA site"
/translation="RRASVA"
CDS 2281..2298
/codon_start=1
/product="thrombin recognition and cleavage site"
/note="thrombin site"
/translation="LVPRGS"
misc_feature 2321..2382
/note="MCS"
/note="multiple cloning site"
misc_recomb 2402..3860
/note="baculovirus recombination region (ORF1629)"
/note="contains part of ORF1629"
CDS complement(2519..3860)
/codon_start=2
/product="baculovirus capsid-associated protein"
/note="ORF1629"
/note="required for viral replication"
/translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
rep_origin 5233..5688
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5970..6074
/gene="bla"
/note="AmpR promoter"
CDS 6075..6935
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 7106..7694
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tcgagcagtt cgttgacgcc ttcctccgtg tggccgaaca cgtcgagcgg gtggtcgatg
61 accagcggcg tgccgcacgc gacgcacaag tatctgtaca ccgaatgatc gtcgggcgaa
121 ggcacgtcgg cctccaagtg gcaatattgg caaattcgaa aatatataca gttgggttgt
181 ttgcgcatat ctatcgtggc gttgggcatg tacgtccgaa cgttgatttg catgcaagcc
241 gaaattaaat cattgcgatt agtgcgatta aaacgttgta catcctcgct tttaatcatg
301 ccgtcgatta aatcgcgcaa tcgagtcaag tgatcaaagt gtggaataat gttttctttg
361 tattcccgag tcaagcgcag cgcgtatttt aacaaactag ccatcttgta agttagtttc
421 atttaatgca actttatcca ataatatatt atgtatcgca cgtcaagaat taacaatgcg
481 cccgttgtcg catctcaaca cgactatgat agagatcaaa taaagcgcga attaaatagc
541 ttgcgacgca acgtgcacga tctgtgcacg cgttccggca cgagctttga ttgtaataag
601 tttttacgaa gcgatgacat gacccccgta gtgacaacga tcacgcccaa aagaactgcc
661 gactacaaaa ttaccgagta tgtcggtgac gttaaaacta ttaagccatc caatcgaccg
721 ttagtcgaat caggaccgct ggtgcgagaa gccgcgaagt atggcgaatg catcgtataa
781 cgtgtggagt ccgctcatta gagcgtcatg tttagacaag aaagctacat atttaattga
841 tcccgatgat tttattgata aattgaccct aactccatac acggtattct acaatggcgg
901 ggttttggtc aaaatttccg gactgcgatt gtacatgctg ttaacggctc cgcccactat
961 taatgaaatt aaaaattcca attttaaaaa acgcagcaag agaaacattt gtatgaaaga
1021 atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac aacaagatta atatgcctcc
1081 gtgtataaaa aaaatattga acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta
1141 caggaagagg tttatactaa actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga
1201 aaaccgatgt ttaatcaagg ctctgacgca tttctacaac cacgactcca agtgtgtggg
1261 tgaagtcatg catcttttaa tcaaatccca agatgtgtat aaaccaccaa actgccaaaa
1321 aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat
1381 ttgtaattat tgaataataa aacaattata aatgctaaat ttgtttttta ttaacgatac
1441 aaaccaaacg caacaagaac atttgtagta ttatctataa ttgaaaacgc gtagttataa
1501 tcgctgaggt aatatttaaa atcattttca aatgattcac agttaatttg cgacaatata
1561 attttatttt cacataaact agacgccttg tcgtcttctt cttcgtattc cttctctttt
1621 tcatttttct cctcataaaa attaacatag ttattatcgt atccatatat gtatctatcg
1681 tatagagtaa attttttgtt gtcataaata tatatgtctt ttttaatggg gtgtatagta
1741 ccgctgcgca tagtttttct gtaatttaca acagtgctat tttctggtag ttcttcggag
1801 tgtgttgctt taattattaa atttatataa tcaatgaatt tgggatcgtc ggttttgtac
1861 aatatgttgc cggcatagta cgcagcttct tctagttcaa ttacaccatt ttttagcagc
1921 accggattaa cataactttc caaaatgttg tacgaaccgt taaacaaaaa cagttcacct
1981 cccttttcta tactattgtc tgcgagcagt tgtttgttgt taaaaataac agccattgta
2041 atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctgata
2101 tcatggagat aattaaaatg ataaccatct cgcaaataaa taagtatttt actgttttcg
2161 taacagtttt gtaataaaaa aacctataaa tacggatctg tattcatgtc ccctatagat
2221 ccgatgggac atcatcatca tcatcacgga aggagaaggg ccagtgttgc ggcgggaatt
2281 ttggtccctc gtggaagccc aggactcgat ggcatatatg ctcgaggaat tcaggcctcc
2341 atgggagctc gcggccgcct gcagggtacc cccgggagat ctgtaccgac tctgctgaag
2401 aggaggaaat tctccttgaa gtttccctgg tgttcaaagt aaaggagttt gcaccagacg
2461 cacctctgtt cactggtccg gcgtattaaa acacgataca ttgttattag tacatttatt
2521 aagcgctaga ttctgtgcgt tgttgattta cagacaattg ttgtacgtat tttaataatt
2581 cattaaattt ataatcttta gggtggtatg ttagagcgaa aatcaaatga ttttcagcgt
2641 ctttatatct gaatttaaat attaaatcct caatagattt gtaaaatagg tttcgattag
2701 tttcaaacaa gggttgtttt tccgaaccga tggctggact atctaatgga ttttcgctca
2761 acgccacaaa acttgccaaa tcttgtagca gcaatctagc tttgtcgata ttcgtttgtg
2821 ttttgttttg taataaaggt tcgacgtcgt tcaaaatatt atgcgctttt gtatttcttt
2881 catcactgtc gttagtgtac aattgactcg acgtaaacac gttaaataaa gcttggacat
2941 atttaacatc gggcgtgtta gctttattag gccgattatc gtcgtcgtcc caaccctcgt
3001 cgttagaagt tgcttccgaa gacgattttg ccatagccac acgacgccta ttaattgtgt
3061 cggctaacac gtccgcgatc aaatttgtag ttgagctttt tggaattatt tctgattgcg
3121 ggcgtttttg ggcgggtttc aatctaactg tgcccgattt taattcagac aacacgttag
3181 aaagcgatgg tgcaggcggt ggtaacattt cagacggcaa atctactaat ggcggcggtg
3241 gtggagctga tgataaatct accatcggtg gaggcgcagg cggggctggc ggcggaggcg
3301 gaggcggagg tggtggcggt gatgcagacg gcggtttagg ctcaaatgtc tctttaggca
3361 acacagtcgg cacctcaact attgtactgg tttcgggcgc cgtttttggt ttgaccggtc
3421 tgagacgagt gcgatttttt tcgtttctaa tagcttccaa caattgttgt ctgtcgtcta
3481 aaggtgcagc gggttgaggt tccgtcggca ttggtggagc gggcggcaat tcagacatcg
3541 atggtggtgg tggtggtgga ggcgctggaa tgttaggcac gggagaaggt ggtggcggcg
3601 gtgccgccgg tataatttgt tctggtttag tttgttcgcg cacgattgtg ggcaccggcg
3661 caggcgccgc tggctgcaca acggaaggtc gtctgcttcg aggcagcgct tggggtggtg
3721 gcaattcaat attataattg gaatacaaat cgtaaaaatc tgctataagc attgtaattt
3781 cgctatcgtt taccgtgccg atatttaaca accgctcaat gtaagcaatt gtattgtaaa
3841 gagattgtct caagctcgga tcgatcccgc acgccgataa caagcctttt catttttact
3901 acagcattgt agtggcgaga cacttcgctg tcgtcgacgt acatgtatgc tttgttgtca
3961 aaaacgtcgt tggcaagctt taaaatattt aaaagaacat ctctgttcag caccactgtg
4021 ttgtcgtaaa tgttgttttt gataatttgc gcttccgcag tatcgacacg ttcaaaaaat
4081 tgatgcgcat caattttgtt gttcctatta ttgaataaat aagattgtac agattcatat
4141 ctacgattcg tcatggccac cacaaatgct acgctgcaaa cgctggtaca attttacgaa
4201 aactgcaaaa acgtcaaaac tcggtataaa ataatcaacg ggcgctttgg caaaatatct
4261 attttatcgc acaagcccac tagcaaattg tatttgcaga aaacaatttc ggcgcacaat
4321 tttaacgctg acgaaataaa agttcaccag ttaatgagcg accacccaaa ttttataaaa
4381 atctatttta atcacggttc catcaacaac caagtgatcg tgatggacta cattgactgt
4441 cccgatttat ttgaaacact acaaattaaa ggcgagcttt cgtaccaact tgttagcaat
4501 attattagac agctgtgtga agcgctcaac gatttgcaca agcacaattt catacacaac
4561 gacataaaac tcgaaaatgt cttatatttc gaagcacttg atcgcgtgta tgtttgcgat
4621 tacggattgt gcaaacacga aaactcactt agcgtgcacg acggcacgtt ggagtatttt
4681 agtccggaaa aaattcgaca cacaactatg cacgtttcgt ttgactggta cgcggcgtgt
4741 taacatacaa gttgctaacc ggcggccgac acccatttga aaaaagcgaa gacgaaatgt
4801 tggacttgaa tagcatgaag cgtcgtcagc aatacaatga cattggcgtt ttaaaacacg
4861 ttcgtaacgt taacgctcgt gactttgtgt actgcctaac aagatacaac atagattgta
4921 gactcacaaa ttacaaacaa attataaaac atgagttttt gtcgtaaaaa tgccacttgt
4981 tttacgagta gaattagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac
5041 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
5101 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
5161 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
5221 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
5281 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
5341 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
5401 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
5461 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
5521 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt
5581 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
5641 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
5701 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
5761 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
5821 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
5881 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
5941 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
6001 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
6061 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
6121 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
6181 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
6241 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
6301 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
6361 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
6421 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
6481 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
6541 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
6601 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
6661 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
6721 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
6781 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
6841 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
6901 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
6961 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
7021 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
7081 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
7141 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
7201 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
7261 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
7321 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
7381 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
7441 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
7501 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
7561 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
7621 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
7681 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
7741 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
7801 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
7861 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
7921 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
7981 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
8041 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
8101 attacgaatt
//
