pAcHLT B

价格:1500元

联系方式:I47-825O-882O

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pAcHLTB载体基本信息

出品公司: BD Biosciences
载体名称: pAcHLT B
质粒类型: 昆虫细胞载体
高拷贝/低拷贝: 高拷贝
克隆方法: --
启动子: --
载体大小: 8110 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: --
克隆菌株: --
宿主细胞(系): --
备注: --
产品目录号: --
稳定性: --
组成型/诱导型: --
病毒/非病毒: --

pAcHLTB载体质粒图谱和多克隆位点信息

pAcHLT B载体图谱

pAcHLTB载体简介

pAcHLTB载体序列

LOCUS       pAcHLT B                8110 bp ds-DNA     circular SYN 19-APR-2014
DEFINITION  Baculovirus transfer vector for expressing proteins with an 
            N-terminal 6xHis-PKA-thrombin cassette. For other reading frames, 
            use pAcHLT A or pAcHLT C.
ACCESSION   .
VERSION     .
KEYWORDS    pAcHLT B
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8110)
  AUTHORS   BD Biosciences
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, February 5, 2020 from SnapGene Viewer 3.1.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8110
                     /organism="synthetic DNA construct"
                     /lab_host="Insect Cells"
                     /mol_type="other DNA"
     misc_recomb     1269..2096
                     /note="baculovirus recombination region (lef2/ORF603)"
                     /note="contains ORF603 and part of lef2"
     CDS             1269..1393
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /note="lef2"
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(1431..2036)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /note="ORF603"
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     promoter        2100..2191
                     /gene="polh from Autographa californica"
                     /note="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             2230..2247
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
     CDS             2254..2271
                     /codon_start=1
                     /product="recognition site for phosphorylation by protein 
                     kinase A"
                     /note="PKA site"
                     /translation="RRASVA"
     CDS             2281..2298
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /note="thrombin site"
                     /translation="LVPRGS"
     misc_feature    2321..2382
                     /note="MCS"
                     /note="multiple cloning site"
     misc_recomb     2402..3860
                     /note="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     CDS             complement(2519..3860)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /note="ORF1629"
                     /note="required for viral replication"
                     /translation="PSLRQSLYNTIAYIERLLNIGTVNDSEITMLIADFYDLYSNYNIE
                     LPPPQALPRSRRPSVVQPAAPAPVPTIVREQTKPEQIIPAAPPPPPSPVPNIPAPPPPP
                     PPSMSELPPAPPMPTEPQPAAPLDDRQQLLEAIRNEKNRTRLRPVKPKTAPETSTIVEV
                     PTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPPMVDLSSAPPPPPLVDLPSEMLPPP
                     APSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSSTTNLIADVLADTINRRRVAMAKSSS
                     EATSNDEGWDDDDNRPNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVE
                     PLLQNKTQTNIDKARLLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDL
                     IFKFRYKDAENHLIFALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     rep_origin      5233..5688
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5970..6074
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             6075..6935
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7106..7694
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tcgagcagtt cgttgacgcc ttcctccgtg tggccgaaca cgtcgagcgg gtggtcgatg
       61 accagcggcg tgccgcacgc gacgcacaag tatctgtaca ccgaatgatc gtcgggcgaa
      121 ggcacgtcgg cctccaagtg gcaatattgg caaattcgaa aatatataca gttgggttgt
      181 ttgcgcatat ctatcgtggc gttgggcatg tacgtccgaa cgttgatttg catgcaagcc
      241 gaaattaaat cattgcgatt agtgcgatta aaacgttgta catcctcgct tttaatcatg
      301 ccgtcgatta aatcgcgcaa tcgagtcaag tgatcaaagt gtggaataat gttttctttg
      361 tattcccgag tcaagcgcag cgcgtatttt aacaaactag ccatcttgta agttagtttc
      421 atttaatgca actttatcca ataatatatt atgtatcgca cgtcaagaat taacaatgcg
      481 cccgttgtcg catctcaaca cgactatgat agagatcaaa taaagcgcga attaaatagc
      541 ttgcgacgca acgtgcacga tctgtgcacg cgttccggca cgagctttga ttgtaataag
      601 tttttacgaa gcgatgacat gacccccgta gtgacaacga tcacgcccaa aagaactgcc
      661 gactacaaaa ttaccgagta tgtcggtgac gttaaaacta ttaagccatc caatcgaccg
      721 ttagtcgaat caggaccgct ggtgcgagaa gccgcgaagt atggcgaatg catcgtataa
      781 cgtgtggagt ccgctcatta gagcgtcatg tttagacaag aaagctacat atttaattga
      841 tcccgatgat tttattgata aattgaccct aactccatac acggtattct acaatggcgg
      901 ggttttggtc aaaatttccg gactgcgatt gtacatgctg ttaacggctc cgcccactat
      961 taatgaaatt aaaaattcca attttaaaaa acgcagcaag agaaacattt gtatgaaaga
     1021 atgcgtagaa ggaaagaaaa atgtcgtcga catgctgaac aacaagatta atatgcctcc
     1081 gtgtataaaa aaaatattga acgatttgaa agaaaacaat gtaccgcgcg gcggtatgta
     1141 caggaagagg tttatactaa actgttacat tgcaaacgtg gtttcgtgtg ccaagtgtga
     1201 aaaccgatgt ttaatcaagg ctctgacgca tttctacaac cacgactcca agtgtgtggg
     1261 tgaagtcatg catcttttaa tcaaatccca agatgtgtat aaaccaccaa actgccaaaa
     1321 aatgaaaact gtcgacaagc tctgtccgtt tgctggcaac tgcaagggtc tcaatcctat
     1381 ttgtaattat tgaataataa aacaattata aatgctaaat ttgtttttta ttaacgatac
     1441 aaaccaaacg caacaagaac atttgtagta ttatctataa ttgaaaacgc gtagttataa
     1501 tcgctgaggt aatatttaaa atcattttca aatgattcac agttaatttg cgacaatata
     1561 attttatttt cacataaact agacgccttg tcgtcttctt cttcgtattc cttctctttt
     1621 tcatttttct cctcataaaa attaacatag ttattatcgt atccatatat gtatctatcg
     1681 tatagagtaa attttttgtt gtcataaata tatatgtctt ttttaatggg gtgtatagta
     1741 ccgctgcgca tagtttttct gtaatttaca acagtgctat tttctggtag ttcttcggag
     1801 tgtgttgctt taattattaa atttatataa tcaatgaatt tgggatcgtc ggttttgtac
     1861 aatatgttgc cggcatagta cgcagcttct tctagttcaa ttacaccatt ttttagcagc
     1921 accggattaa cataactttc caaaatgttg tacgaaccgt taaacaaaaa cagttcacct
     1981 cccttttcta tactattgtc tgcgagcagt tgtttgttgt taaaaataac agccattgta
     2041 atgagacgca caaactaata tcacaaactg gaaatgtcta tcaatatata gttgctgata
     2101 tcatggagat aattaaaatg ataaccatct cgcaaataaa taagtatttt actgttttcg
     2161 taacagtttt gtaataaaaa aacctataaa tacggatctg tattcatgtc ccctatagat
     2221 ccgatgggac atcatcatca tcatcacgga aggagaaggg ccagtgttgc ggcgggaatt
     2281 ttggtccctc gtggaagccc aggactcgat ggcatatatg ctcgaggaat tcaggcctcc
     2341 atgggagctc gcggccgcct gcagggtacc cccgggagat ctgtaccgac tctgctgaag
     2401 aggaggaaat tctccttgaa gtttccctgg tgttcaaagt aaaggagttt gcaccagacg
     2461 cacctctgtt cactggtccg gcgtattaaa acacgataca ttgttattag tacatttatt
     2521 aagcgctaga ttctgtgcgt tgttgattta cagacaattg ttgtacgtat tttaataatt
     2581 cattaaattt ataatcttta gggtggtatg ttagagcgaa aatcaaatga ttttcagcgt
     2641 ctttatatct gaatttaaat attaaatcct caatagattt gtaaaatagg tttcgattag
     2701 tttcaaacaa gggttgtttt tccgaaccga tggctggact atctaatgga ttttcgctca
     2761 acgccacaaa acttgccaaa tcttgtagca gcaatctagc tttgtcgata ttcgtttgtg
     2821 ttttgttttg taataaaggt tcgacgtcgt tcaaaatatt atgcgctttt gtatttcttt
     2881 catcactgtc gttagtgtac aattgactcg acgtaaacac gttaaataaa gcttggacat
     2941 atttaacatc gggcgtgtta gctttattag gccgattatc gtcgtcgtcc caaccctcgt
     3001 cgttagaagt tgcttccgaa gacgattttg ccatagccac acgacgccta ttaattgtgt
     3061 cggctaacac gtccgcgatc aaatttgtag ttgagctttt tggaattatt tctgattgcg
     3121 ggcgtttttg ggcgggtttc aatctaactg tgcccgattt taattcagac aacacgttag
     3181 aaagcgatgg tgcaggcggt ggtaacattt cagacggcaa atctactaat ggcggcggtg
     3241 gtggagctga tgataaatct accatcggtg gaggcgcagg cggggctggc ggcggaggcg
     3301 gaggcggagg tggtggcggt gatgcagacg gcggtttagg ctcaaatgtc tctttaggca
     3361 acacagtcgg cacctcaact attgtactgg tttcgggcgc cgtttttggt ttgaccggtc
     3421 tgagacgagt gcgatttttt tcgtttctaa tagcttccaa caattgttgt ctgtcgtcta
     3481 aaggtgcagc gggttgaggt tccgtcggca ttggtggagc gggcggcaat tcagacatcg
     3541 atggtggtgg tggtggtgga ggcgctggaa tgttaggcac gggagaaggt ggtggcggcg
     3601 gtgccgccgg tataatttgt tctggtttag tttgttcgcg cacgattgtg ggcaccggcg
     3661 caggcgccgc tggctgcaca acggaaggtc gtctgcttcg aggcagcgct tggggtggtg
     3721 gcaattcaat attataattg gaatacaaat cgtaaaaatc tgctataagc attgtaattt
     3781 cgctatcgtt taccgtgccg atatttaaca accgctcaat gtaagcaatt gtattgtaaa
     3841 gagattgtct caagctcgga tcgatcccgc acgccgataa caagcctttt catttttact
     3901 acagcattgt agtggcgaga cacttcgctg tcgtcgacgt acatgtatgc tttgttgtca
     3961 aaaacgtcgt tggcaagctt taaaatattt aaaagaacat ctctgttcag caccactgtg
     4021 ttgtcgtaaa tgttgttttt gataatttgc gcttccgcag tatcgacacg ttcaaaaaat
     4081 tgatgcgcat caattttgtt gttcctatta ttgaataaat aagattgtac agattcatat
     4141 ctacgattcg tcatggccac cacaaatgct acgctgcaaa cgctggtaca attttacgaa
     4201 aactgcaaaa acgtcaaaac tcggtataaa ataatcaacg ggcgctttgg caaaatatct
     4261 attttatcgc acaagcccac tagcaaattg tatttgcaga aaacaatttc ggcgcacaat
     4321 tttaacgctg acgaaataaa agttcaccag ttaatgagcg accacccaaa ttttataaaa
     4381 atctatttta atcacggttc catcaacaac caagtgatcg tgatggacta cattgactgt
     4441 cccgatttat ttgaaacact acaaattaaa ggcgagcttt cgtaccaact tgttagcaat
     4501 attattagac agctgtgtga agcgctcaac gatttgcaca agcacaattt catacacaac
     4561 gacataaaac tcgaaaatgt cttatatttc gaagcacttg atcgcgtgta tgtttgcgat
     4621 tacggattgt gcaaacacga aaactcactt agcgtgcacg acggcacgtt ggagtatttt
     4681 agtccggaaa aaattcgaca cacaactatg cacgtttcgt ttgactggta cgcggcgtgt
     4741 taacatacaa gttgctaacc ggcggccgac acccatttga aaaaagcgaa gacgaaatgt
     4801 tggacttgaa tagcatgaag cgtcgtcagc aatacaatga cattggcgtt ttaaaacacg
     4861 ttcgtaacgt taacgctcgt gactttgtgt actgcctaac aagatacaac atagattgta
     4921 gactcacaaa ttacaaacaa attataaaac atgagttttt gtcgtaaaaa tgccacttgt
     4981 tttacgagta gaattagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     5041 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     5101 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     5161 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
     5221 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     5281 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     5341 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     5401 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
     5461 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     5521 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt
     5581 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     5641 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
     5701 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     5761 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     5821 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     5881 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     5941 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     6001 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     6061 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6121 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6181 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6241 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6301 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6361 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6421 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6481 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6541 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     6601 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     6661 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     6721 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     6781 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     6841 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     6901 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     6961 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     7021 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     7081 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7141 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7201 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     7261 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7321 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7381 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7441 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7501 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7561 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7621 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7681 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7741 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     7801 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     7861 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     7921 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     7981 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     8041 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     8101 attacgaatt
//

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