pET-51b(+)

价格:800元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Novangen
载体名称: pET-51b
质粒类型: 大肠杆菌表达载体;蛋白纯化
高拷贝/低拷贝: 低拷贝
克隆方法: 多克隆位点;限制性内切酶
启动子: T7
载体大小: 5218 bp
5' 测序引物及序列: T7 Fwd:  5'd[TAATACGACTCACTATAGGG]3'
3' 测序引物及序列: --
载体标签: His Tag (C-末端);Strep TagII (N-末端)
载体抗性: 氨苄青霉素(Ampicillin)
表达宿主菌: BL21(DE3) Rosetta2(DE3)C43(DE3)
备注: Strep tagII 下游含EK蛋白酶识别位点。
产品目录号: 71553-3
稳定性: 稳表达
组成型/诱导型: 诱导型(IPTG)
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pET-51b载体图谱



pET-51b多克隆位点

pET-51b载体特征

载体简介

The pET-51b(+) vector carries an N-terminal Strep•Tag® II coding sequence (1) followed by a recognition site for enterokinase, and an optional C-terminal His•Tag® coding sequence. The presence of two "gentle elution" tags at both the N-terminus and C-terminus is ideal for dual purification strategies designed to isolate full-length fusion proteins (2). Unique restriction sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circle map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below the circle map. The f1 origin is oriented so that infection with the helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.


 
pET Vectors 选择指南

载体序列

LOCUS       pET-51b(+)   5218 bp    DNA   circular             1-SEP-2005
DEFINITION  pET-51b(+) vector sequence.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pET-51b(+)|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
FEATURES             Location/Qualifiers
     misc_RNA        complement(1656..1763)
                     /vntifkey="53"
                     /label=RNA\I
     misc_RNA        1648..2203
                     /vntifkey="53"
                     /label=RNA\II
     CDS             complement(2634..2825)
                     /vntifkey="4"
                     /label=ROP
     RBS             4944..4949
                     /vntifkey="32"
                     /label=RBS-1
     primer          4824..4839
                     /vntifkey="27"
                     /label=pET\Upstream\Primer
     promoter        4870..4886
                     /vntifkey="30"
                     /label=T7\promoter
     CDS             complement(3397..4479)
                     /vntifkey="4"
                     /label=lac\I
     rep_origin      2203..2203
                     /vntifkey="33"
                     /label=ColE1\pBR322\origin
     CDS             complement(582..1442)
                     /vntifkey="4"
                     /label=bla\(amp)\sequence
     rep_origin      13..460
                     /vntifkey="33"
                     /label=f1\origin
     CDS             4890..4911
                     /vntifkey="4"
                     /label=lac\operator
     terminator      5146..5193
                     /vntifkey="43"
                     /label=T7\Terminator
     -35_signal      1616..1621
                     /vntifkey="48"
                     /label=-35\RNA\II
     -10_signal      1639..1644
                     /vntifkey="47"
                     /label=-10\RNA\II
     misc_RNA        1652..2203
                     /vntifkey="53"
                     /label=RNA\II
     -35_signal      complement(1793..1798)
                     /vntifkey="48"
                     /label=-35\RNA\I
     -10_signal      complement(1769..1774)
                     /vntifkey="47"
                     /label=-10\RNA\I
     misc_RNA        complement(1626..1762)
                     /vntifkey="53"
                     /label=RNA\I
     terminator      1607..1625
                     /vntifkey="43"
                     /label=RNA\I\Terminator
     misc_feature    5056..5070
                     /vntifkey="21"
                     /label=C-Term\Ek/LIC\overhang
     protein_bind    4996..5010
                     /vntifkey="31"
                     /label=Ek\site
     protein_bind    4966..4989
                     /vntifkey="31"
                     /label=Strep-Tag\II
     misc_binding    5080..5110
                     /vntifkey="20"
                     /label=10x\His-Tag
     primer          4990..5073
                     /vntifkey="27"
                     /label=S-EkLIC\MCS
     primer          complement(4996..5079)
                     /vntifkey="27"
                     /label=AS-EkLIC\MCS
     primer          4956..4989
                     /vntifkey="27"
                     /label=S-NTerm\Strep
     primer          complement(4960..4995)
                     /vntifkey="27"
                     /label=AS-NTerm\Strep
BASE COUNT     1208 a      1398 c      1352 g      1260 t 
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttctg gcggcacgat 
      481 ggcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 
      541 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 
      601 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 
      661 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 
      721 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 
      781 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 
      841 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 
      901 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 
      961 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 
     1021 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 
     1081 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 
     1141 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 
     1201 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 
     1261 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 
     1321 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 
     1381 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 
     1441 atactcttcc tttttcaatc atgattgaag catttatcag ggttattgtc tcatgagcgg 
     1501 atacatattt gaatgtattt agaaaaataa acaaataggt catgaccaaa atcccttaac 
     1561 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 
     1621 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 
     1681 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 
     1741 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga 
     1801 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 
     1861 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 
     1921 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 
     1981 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 
     2041 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 
     2101 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 
     2161 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 
     2221 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 
     2281 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca 
     2341 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt 
     2401 attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac 
     2461 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg 
     2521 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg 
     2581 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg 
     2641 ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg 
     2701 tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga 
     2761 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg 
     2821 gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa 
     2881 cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt 
     2941 tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt 
     3001 caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct 
     3061 gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac 
     3121 gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag 
     3181 cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc 
     3241 cgccagccta gccgggtcct caacgacagg agcacgatca tgctagtcat gccccgcgcc 
     3301 caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc 
     3361 taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 
     3421 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt 
     3481 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt 
     3541 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg 
     3601 aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc 
     3661 gtatcccact accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat 
     3721 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt 
     3781 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc 
     3841 tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc 
     3901 cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag 
     3961 atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt 
     4021 ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat 
     4081 ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag 
     4141 attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac 
     4201 gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg 
     4261 cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg 
     4321 tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt 
     4381 tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc 
     4441 ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact 
     4501 ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg 
     4561 gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga 
     4621 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc 
     4681 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt 
     4741 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg 
     4801 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc gatctcgatc 
     4861 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag 
     4921 aaataatttt gtttaacttt aagaaggaga tataccatgg caagctggag ccacccgcag 
     4981 ttcgaaaagg gtgcagatga cgacgacaag gtaccggatc cgacgtcggt cgacaagctt 
     5041 gcggccgcag gagctccggg cttctcctca atttccgctc atcaccacca tcatcaccat 
     5101 caccaccact aattaaccta ggctgctgcc accgctgagc aataactagc ataacccctt 
     5161 ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggat 
//

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