| 出品公司: | Novagen |
|---|---|
| 载体名称: | pET-51b(+) , pET51b , pET51b载体 , pET-51b载体 |
| 质粒类型: | 大肠杆菌表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | T7 |
| 载体大小: | 5.2 kb |
| 5’测序引物及序列: | T7: 5'-TAATACGACTCACTATAGGG-3' |
| 3’测序引物及序列: | T7t: 5'-GCTAGTTATTGCTCAGCGG-3' |
| 载体标签: | N-Strep tag , C-His tag |
| 载体抗性: | 氨苄青霉素 |
| 克隆菌株: | DH5 alpha |
| 表达细胞: | BL21(DE3), Rosetta(DE3), OrigamiB(DE3) |
| 备注: | |
| 产品目录号: | 71553 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
pET-51b(+)
价格：1500元
联系方式：I47-825O-882O
买家导航
pET51b载体基本信息
pET51b载体质粒图谱和多克隆位点信息
pET51b载体简介
pET-51b（+）载体携带N-Strep Tag，N-EK蛋白酶位点和可选的C-His Tag。存在N端和C端的两个纯化标签是双重纯化策略，本设计用于分离全长融合蛋白。pET51b载体的单一的多克隆位点见上面的环状质粒图谱。注意：载体序列是以pBR322质粒的编码规矩进行编码的，所以T7蛋白表达区在质粒图谱上面是反向的。T7 RNA聚合酶启动的克隆和表达区域在质粒图谱中也被标注了出来。质粒的F1复制子是被定向的，所以在T7噬菌体聚合酶的作用下，包含有蛋白编码序列的病毒粒子能够产生，并启动蛋白表达，同时蛋白表达将被T7终止子序列(Cat. No. 69337-3)的作用下终止蛋白翻译。
pET51b载体序列
LOCUS pET-51b(+) 5218 bp DNA circular 1-SEP-2005
DEFINITION Reverse complement of pET-51b.
SOURCE
ORGANISM
COMMENT VNTNAME|pET-51b(+) rc|
COMMENT VNTREPLTYPE|Plasmid
COMMENT VNTEXTCHREPL|Bacteria
COMMENT http://www.biofeng.com
FEATURES Location/Qualifiers
misc_RNA 3456..3563
/vntifkey="53"
/label=RNA\I
misc_RNA complement(3016..3571)
/vntifkey="53"
/label=RNA\II
CDS 2394..2585
/vntifkey="4"
/label=ROP
RBS complement(270..275)
/vntifkey="32"
/label=RBS-1
primer complement(380..395)
/vntifkey="27"
/label=pET\Upstream\Primer
promoter complement(333..349)
/vntifkey="30"
/label=T7\promoter
CDS 740..1822
/vntifkey="4"
/label=lac\I
rep_origin complement(3016..3016)
/vntifkey="33"
/label=ColE1\pBR322\origin
CDS 3777..4637
/vntifkey="4"
/label=bla\(amp)\sequence
rep_origin complement(4759..5206)
/vntifkey="33"
/label=f1\origin
CDS complement(308..329)
/vntifkey="4"
/label=lac\operator
terminator complement(26..73)
/vntifkey="43"
/label=T7\Terminator
-35_signal complement(3598..3603)
/vntifkey="48"
/label=-35\RNA\II
-10_signal complement(3575..3580)
/vntifkey="47"
/label=-10\RNA\II
misc_RNA complement(3016..3567)
/vntifkey="53"
/label=RNA\II
-35_signal 3421..3426
/vntifkey="48"
/label=-35\RNA\I
-10_signal 3445..3450
/vntifkey="47"
/label=-10\RNA\I
misc_RNA 3457..3593
/vntifkey="53"
/label=RNA\I
terminator complement(3594..3612)
/vntifkey="43"
/label=RNA\I\Terminator
misc_feature complement(149..163)
/vntifkey="21"
/label=C-Term\Ek/LIC\overhang
protein_bind complement(209..223)
/vntifkey="31"
/label=Ek\site
protein_bind complement(230..253)
/vntifkey="31"
/label=Strep-Tag\II
misc_binding complement(109..139)
/vntifkey="20"
/label=10x\His-Tag
primer complement(146..229)
/vntifkey="27"
/label=S-EkLIC\MCS
primer 140..223
/vntifkey="27"
/label=AS-EkLIC\MCS
primer complement(230..263)
/vntifkey="27"
/label=S-NTerm\Strep
primer 224..259
/vntifkey="27"
/label=AS-NTerm\Strep
BASE COUNT 1260 a 1352 c 1398 g 1208 t
ORIGIN
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccta ggttaattag tggtggtgat
121 ggtgatgatg gtggtgatga gcggaaattg aggagaagcc cggagctcct gcggccgcaa
181 gcttgtcgac cgacgtcgga tccggtacct tgtcgtcgtc atctgcaccc ttttcgaact
241 gcgggtggct ccagcttgcc atggtatatc tccttcttaa agttaaacaa aattatttct
301 agaggggaat tgttatccgc tcacaattcc cctatagtga gtcgtattaa tttcgcggga
361 tcgagatcga tctcgatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca
421 ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac
481 ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga
541 ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc
601 aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgagatccc
661 ggacaccatc gaatggcgca aaacctttcg cggtatggca tgatagcgcc cggaagagag
721 tcaattcagg gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg
781 tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac
841 gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca
901 acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca
961 cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt
1021 ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct
1081 tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat
1141 tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac
1201 acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct
1261 ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc
1321 gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc
1381 ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa
1441 tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat
1501 gcgcgccatt accgagtccg ggctgcgcgt tggtgcggac atctcggtag tgggatacga
1561 cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg
1621 cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa
1681 gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac
1741 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
1801 ccgactggaa agcgggcagt gagcgcaacg caattaatgt aagttagctc actcattagg
1861 caccgggatc tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg
1921 cgcggggcat gactagcatg atcgtgctcc tgtcgttgag gacccggcta ggctggcggg
1981 gttgccttac tggttagcag aatgaatcac cgatacgcga gcgaacgtga agcgactgct
2041 gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt
2101 aaagtctgga aacgcggaag tcagcgccct gcaccattat gttccggatc tgcatcgcag
2161 gatgctgctg gctaccctgt ggaacaccta catctgtatt aacgaagcgc tggcattgac
2221 cctgagtgat ttttctctgg tcccgccgca tccataccgc cagttgttta ccctcacaac
2281 gttccagtaa ccgggcatgt tcatcatcag taacccgtat cgtgagcatc ctctctcgtt
2341 tcatcggtat cattaccccc atgaacagaa atccccctta cacggaggca tcagtgacca
2401 aacaggaaaa aaccgccctt aacatggccc gctttatcag aagccagaca ttaacgcttc
2461 tggagaaact caacgagctg gacgcggatg aacaggcaga catctgtgaa tcgcttcacg
2521 accacgctga tgagctttac cgcagctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
2581 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
2641 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
2701 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
2761 actgagagtg caccatatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
2821 cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
2881 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
2941 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
3001 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
3061 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
3121 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
3181 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
3241 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
3301 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
3361 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
3421 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
3481 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
3541 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
3601 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
3661 taagggattt tggtcatgac ctatttgttt atttttctaa atacattcaa atatgtatcc
3721 gctcatgaga caataaccct gataaatgct tcaatcatga ttgaaaaagg aagagtatga
3781 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
3841 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
3901 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
3961 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
4021 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
4081 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
4141 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
4201 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
4261 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
4321 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
4381 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
4441 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
4501 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
4561 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
4621 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
4681 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgccat
4741 cgtgccgcca gaaattgtaa acgttaatat tttgttaaaa ttcgcgttaa atttttgtta
4801 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
4861 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
4921 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
4981 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
5041 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
5101 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
5161 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgcca
//
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