买家导航
pcDNA3.1+C-6HIS载体质粒基本信息
pcDNA3.1+C-6HIS质粒图谱载体图谱和pcDNA3.1+C-6HIS载体序列质粒序列多克隆位点信息
pcDNA3.1+C-6HIS质粒载体简介
pcDNA3.1+C-6HIS质粒序列载体序列
LOCUS pcDNA3.1+C-6HIS 5435 bp DNA circular SYN 17-NOV-2022
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTDATE|-14661511|
COMMENT VNTDBDATE|-14661511|
COMMENT LSOWNER|
COMMENT VNTNAME|pcDNA3.1+C-6HIS|
COMMENT VNTAUTHORNAME|Biofeng.com|
COMMENT VNTAUTHOREML|order@biofeng.com|
COMMENT VNTOAUTHORNAME|Invitrogen|
COMMENT VNTOAUTHORTEL|(800)-357-3114|
COMMENT VNTOAUTHORFAX|(760)-602-6500|
COMMENT VNTOAUTHOREML|bioinfosales@invitrogen.com|
COMMENT VNTOAUTHORAD1|Invitrogen Corporation|
COMMENT VNTOAUTHORAD2|1620 Faraday Avenue|
COMMENT VNTOAUTHORAD3|Carlsbad, CA 92008 U.S.A.|
FEATURES Location/Qualifiers
source 1..5435
/invitrogen="124"
/vntifkey="98"
CDS complement(4439..5299)
/invitrogen="1020000"
/vntifkey="4"
/label=Amp(R)
polyA_site 1035..1259
/invitrogen="1870000"
/vntifkey="26"
/label=BGH\pA
primer complement(1029..1046)
/invitrogen="1910000"
/vntifkey="27"
/label=BGH\reverse\primer
promoter complement(5300..5398)
/invitrogen="2330000"
/vntifkey="30"
/label=bla\promoter
primer 769..789
/invitrogen="1930000"
/vntifkey="27"
/label=CMV\forward\primer
promoter 232..819
/invitrogen="2180000"
/vntifkey="29"
/label=CMV\promoter
rep_origin 1305..1733
/invitrogen="2500000"
/vntifkey="33"
/label=f1\origin
misc_feature 895..985
/invitrogen="1630000"
/vntifkey="21"
/label=MCS
CDS 2143..2937
/invitrogen="1260000"
/vntifkey="4"
/label=Neo(R)
rep_origin complement(3624..4294)
/invitrogen="2540000"
/vntifkey="33"
/label=pUC\origin
promoter 1738..2107
/invitrogen="2290000"
/vntifkey="29"
/label=SV40\early\promoter
polyA_signal 3111..3241
/invitrogen="1840000"
/vntifkey="25"
/label=SV40\pA
primer 863..882
/invitrogen="2100000"
/vntifkey="27"
/label=T7\primer
promoter 863..879
/invitrogen="2370000"
/vntifkey="30"
/label=T7\promoter
misc_feature 986..1003
/vntifkey="21"
/label=His\Tag
BASE COUNT 1257 a 1416 c 1381 g 1381 t
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttggta ccgagctcgg atccgaattc tgcagatatc cagcacagtg
961 gcggccgctc gagtctagag ggccccatca tcaccatcac cattgataag tttaaacccg
1021 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
1081 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
1141 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
1201 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
1261 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
1321 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
1381 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
1441 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
1501 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
1561 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
1621 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
1681 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
1741 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
1801 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
1861 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
1921 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
1981 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
2041 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
2101 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
2161 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
2221 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
2281 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
2341 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
2401 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
2461 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
2521 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
2581 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
2641 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
2701 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
2761 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
2821 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
2881 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
2941 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
3001 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
3061 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
3121 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
3181 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
3241 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
3301 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
3361 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
3421 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
3481 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
3541 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
3601 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
3661 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
3721 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
3781 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
3841 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
3901 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
3961 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
4021 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
4081 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
4141 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
4201 aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
4261 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
4321 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
4381 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
4441 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
4501 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
4561 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
4621 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
4681 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
4741 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
4801 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
4861 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
4921 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
4981 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
5041 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
5101 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
5161 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
5221 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
5281 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
5341 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
5401 cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
pcDNA3.1+C-6HIS载体图谱质粒图谱pdf版和pcDNA3.1+C-6HIS载体序列质粒序列等相关资料下载
pcDNA3.1+C-6HIS质粒载体应用举例
- 上一篇：pcDNA3.1+N-HA
- 下一篇：pcDNA3.1+N-6HIS
