pwtCas9-bacteria

价格:1000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Addgene
载体名称: pwtCas9-bacteria
质粒类型: CRISPR/Cas9载体;细菌基因敲除载体
高拷贝/低拷贝: 高拷贝
插入位点: 限制性内切酶,多克隆位点
启动子: TetR
载体大小: 6932 bp
5' 测序引物及序列: agaaaagaattcaaaAGATCTaaagaggag-
aaaGGATCTATGGATAAGAAATACTCAATAGG
3' 测序引物及序列: ggagatccttactcgagttaGTCAC-
CTCCTAGCTGACTCAAATCAAT
载体标签: mRFP (N-ter)
载体抗性: 氨苄青霉素、四环素
克隆菌株: Top10
宿主细胞(系): 细菌,如大肠杆菌
备注:  
产品目录号: #44250
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pwtCas9-bacteria 载体图谱



载体简介

载体序列

LOCUS       pwtCas9-bacteria	6932 bp 	DNA	SYN
DEFINITION  pwtCas9-bacteria
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6932
                     /organism="pwtCas9-bacteria"
                     /mol_type="other DNA"
     CDS             complement(7..633)
                     /label="ORF frame 3"
     misc_feature    complement(21..627)
                     /label="TetR"
     CDS             740..4846
                     /label="ORF frame 2"
     terminator      4873..4916
                     /label="rrnB_T1_terminator"
     gene            complement(5951..6811)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(5951..6811)
                     /label="ORF frame 2"
     promoter        complement(6853..6881)
                     /label="AmpR_promoter"
ORIGIN
    1 GCATGCTTAA GACCCACTTT CACATTTAAG TTGTTTTTCT aatccgcata tgatcaattc
   61 aaggccgaat aagaaggctg gctctgcacc ttggtgatca aataattcga tagcttgtcg
  121 taataatggc ggcatactat cagtagtagg tgtttccctt tcttctttag cgacttgatg
  181 ctcttgatct tccaatacgc aacctaaagt aaaatgcccc acagcgctga gtgcatataa
  241 tgcattctct agtgaaaaac cttgttggca taaaaaggct aattgatttt cgagagtttc
  301 atactgtttt tctgtaggcc gtgtacctaa atgtactttt gctccatcgc gatgacttag
  361 taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc tttccccttc
  421 taaagggcaa aagtgagtat ggtgcctatc taacatctca atggctaagg cgtcgagcaa
  481 agcccgctta ttttttacat gccaatacaa tgtaggctgc tctacaccta gcttctgggc
  541 gagtttacgg gttgttaaac cttcgattcc gacctcatta agcagctcta atgcgctgtt
  601 aatcacttta cttttatcta atctagacat cattaattcc taatttttgt tgacactcta
  661 tcgttgatag agttatttta ccactcccta tcagtgatag agaaaagaat tcaaaAGATC
  721 Taaagaggag aaaGGATCTA TGGATAAGAA ATACTCAATA GGCTTAGATA TCGGCACAAA
  781 TAGCGTCGGA TGGGCGGTGA TCACTGATGA ATATAAGGTT CCGTCTAAAA AGTTCAAGGT
  841 TCTGGGAAAT ACAGACCGCC ACAGTATCAA AAAAAATCTT ATAGGGGCTC TTTTATTTGA
  901 CAGTGGAGAG ACAGCGGAAG CGACTCGTCT CAAACGGACA GCTCGTAGAA GGTATACACG
  961 TCGGAAGAAT CGTATTTGTT ATCTACAGGA GATTTTTTCA AATGAGATGG CGAAAGTAGA
 1021 TGATAGTTTC TTTCATCGAC TTGAAGAGTC TTTTTTGGTG GAAGAAGACA AGAAGCATGA
 1081 ACGTCATCCT ATTTTTGGAA ATATAGTAGA TGAAGTTGCT TATCATGAGA AATATCCAAC
 1141 TATCTATCAT CTGCGAAAAA AATTGGTAGA TTCTACTGAT AAAGCGGATT TGCGCTTAAT
 1201 CTATTTGGCC TTAGCGCATA TGATTAAGTT TCGTGGTCAT TTTTTGATTG AGGGAGATTT
 1261 AAATCCTGAT AATAGTGATG TGGACAAACT ATTTATCCAG TTGGTACAAA CCTACAATCA
 1321 ATTATTTGAA GAAAACCCTA TTAACGCAAG TGGAGTAGAT GCTAAAGCGA TTCTTTCTGC
 1381 ACGATTGAGT AAATCAAGAC GATTAGAAAA TCTCATTGCT CAGCTCCCCG GTGAGAAGAA
 1441 AAATGGCTTA TTTGGGAATC TCATTGCTTT GTCATTGGGT TTGACCCCTA ATTTTAAATC
 1501 AAATTTTGAT TTGGCAGAAG ATGCTAAATT ACAGCTTTCA AAAGATACTT ACGATGATGA
 1561 TTTAGATAAT TTATTGGCGC AAATTGGAGA TCAATATGCT GATTTGTTTT TGGCAGCTAA
 1621 GAATTTATCA GATGCTATTT TACTTTCAGA TATCCTAAGA GTAAATACTG AAATAACTAA
 1681 GGCTCCCCTA TCAGCTTCAA TGATTAAACG CTACGATGAA CATCATCAAG ACTTGACTCT
 1741 TTTAAAAGCT TTAGTTCGAC AACAACTTCC AGAAAAGTAT AAAGAAATCT TTTTTGATCA
 1801 ATCAAAAAAC GGATATGCAG GTTATATTGA TGGGGGAGCT AGCCAAGAAG AATTTTATAA
 1861 ATTTATCAAA CCAATTTTAG AAAAAATGGA TGGTACTGAG GAATTATTGG TGAAACTAAA
 1921 TCGTGAAGAT TTGCTGCGCA AGCAACGGAC CTTTGACAAC GGCTCTATTC CCCATCAAAT
 1981 TCACTTGGGT GAGCTGCATG CTATTTTGAG AAGACAAGAA GACTTTTATC CATTTTTAAA
 2041 AGACAATCGT GAGAAGATTG AAAAAATCTT GACTTTTCGA ATTCCTTATT ATGTTGGTCC
 2101 ATTGGCGCGT GGCAATAGTC GTTTTGCATG GATGACTCGG AAGTCTGAAG AAACAATTAC
 2161 CCCATGGAAT TTTGAAGAAG TTGTCGATAA AGGTGCTTCA GCTCAATCAT TTATTGAACG
 2221 CATGACAAAC TTTGATAAAA ATCTTCCAAA TGAAAAAGTA CTACCAAAAC ATAGTTTGCT
 2281 TTATGAGTAT TTTACGGTTT ATAACGAATT GACAAAGGTC AAATATGTTA CTGAAGGAAT
 2341 GCGAAAACCA GCATTTCTTT CAGGTGAACA GAAGAAAGCC ATTGTTGATT TACTCTTCAA
 2401 AACAAATCGA AAAGTAACCG TTAAGCAATT AAAAGAAGAT TATTTCAAAA AAATAGAATG
 2461 TTTTGATAGT GTTGAAATTT CAGGAGTTGA AGATAGATTT AATGCTTCAT TAGGTACCTA
 2521 CCATGATTTG CTAAAAATTA TTAAAGATAA AGATTTTTTG GATAATGAAG AAAATGAAGA
 2581 TATCTTAGAG GATATTGTTT TAACATTGAC CTTATTTGAA GATAGGGAGA TGATTGAGGA
 2641 AAGACTTAAA ACATATGCTC ACCTCTTTGA TGATAAGGTG ATGAAACAGC TTAAACGTCG
 2701 CCGTTATACT GGTTGGGGAC GTTTGTCTCG AAAATTGATT AATGGTATTA GGGATAAGCA
 2761 ATCTGGCAAA ACAATATTAG ATTTTTTGAA ATCAGATGGT TTTGCCAATC GCAATTTTAT
 2821 GCAGCTGATC CATGATGATA GTTTGACATT TAAAGAAGAC ATTCAAAAAG CACAAGTGTC
 2881 TGGACAAGGC GATAGTTTAC ATGAACATAT TGCAAATTTA GCTGGTAGCC CTGCTATTAA
 2941 AAAAGGTATT TTACAGACTG TAAAAGTTGT TGATGAATTG GTCAAAGTAA TGGGGCGGCA
 3001 TAAGCCAGAA AATATCGTTA TTGAAATGGC ACGTGAAAAT CAGACAACTC AAAAGGGCCA
 3061 GAAAAATTCG CGAGAGCGTA TGAAACGAAT CGAAGAAGGT ATCAAAGAAT TAGGAAGTCA
 3121 GATTCTTAAA GAGCATCCTG TTGAAAATAC TCAATTGCAA AATGAAAAGC TCTATCTCTA
 3181 TTATCTCCAA AATGGAAGAG ACATGTATGT GGACCAAGAA TTAGATATTA ATCGTTTAAG
 3241 TGATTATGAT GTCGATCACA TTGTTCCACA AAGTTTCCTT AAAGACGATT CAATAGACAA
 3301 TAAGGTCTTA ACGCGTTCTG ATAAAAATCG TGGTAAATCG GATAACGTTC CAAGTGAAGA
 3361 AGTAGTCAAA AAGATGAAAA ACTATTGGAG ACAACTTCTA AACGCCAAGT TAATCACTCA
 3421 ACGTAAGTTT GATAATTTAA CGAAAGCTGA ACGTGGAGGT TTGAGTGAAC TTGATAAAGC
 3481 TGGTTTTATC AAACGCCAAT TGGTTGAAAC TCGCCAAATC ACTAAGCATG TGGCACAAAT
 3541 TTTGGATAGT CGCATGAATA CTAAATACGA TGAAAATGAT AAACTTATTC GAGAGGTTAA
 3601 AGTGATTACC TTAAAATCTA AATTAGTTTC TGACTTCCGA AAAGATTTCC AATTCTATAA
 3661 AGTACGTGAG ATTAACAATT ACCATCATGC CCATGATGCG TATCTAAATG CCGTCGTTGG
 3721 AACTGCTTTG ATTAAGAAAT ATCCAAAACT TGAATCGGAG TTTGTCTATG GTGATTATAA
 3781 AGTTTATGAT GTTCGTAAAA TGATTGCTAA GTCTGAGCAA GAAATAGGCA AAGCAACCGC
 3841 AAAATATTTC TTTTACTCTA ATATCATGAA CTTCTTCAAA ACAGAAATTA CACTTGCAAA
 3901 TGGAGAGATT CGCAAACGCC CTCTAATCGA AACTAATGGG GAAACTGGAG AAATTGTCTG
 3961 GGATAAAGGG CGAGATTTTG CCACAGTGCG CAAAGTATTG TCCATGCCCC AAGTCAATAT
 4021 TGTCAAGAAA ACAGAAGTAC AGACAGGCGG ATTCTCCAAG GAGTCAATTT TACCAAAAAG
 4081 AAATTCGGAC AAGCTTATTG CTCGTAAAAA AGACTGGGAT CCAAAAAAAT ATGGTGGTTT
 4141 TGATAGTCCA ACGGTAGCTT ATTCAGTCCT AGTGGTTGCT AAGGTGGAAA AAGGGAAATC
 4201 GAAGAAGTTA AAATCCGTTA AAGAGTTACT AGGGATCACA ATTATGGAAA GAAGTTCCTT
 4261 TGAAAAAAAT CCGATTGACT TTTTAGAAGC TAAAGGATAT AAGGAAGTTA AAAAAGACTT
 4321 AATCATTAAA CTACCTAAAT ATAGTCTTTT TGAGTTAGAA AACGGTCGTA AACGGATGCT
 4381 GGCTAGTGCC GGAGAATTAC AAAAAGGAAA TGAGCTGGCT CTGCCAAGCA AATATGTGAA
 4441 TTTTTTATAT TTAGCTAGTC ATTATGAAAA GTTGAAGGGT AGTCCAGAAG ATAACGAACA
 4501 AAAACAATTG TTTGTGGAGC AGCATAAGCA TTATTTAGAT GAGATTATTG AGCAAATCAG
 4561 TGAATTTTCT AAGCGTGTTA TTTTAGCAGA TGCCAATTTA GATAAAGTTC TTAGTGCATA
 4621 TAACAAACAT AGAGACAAAC CAATACGTGA ACAAGCAGAA AATATTATTC ATTTATTTAC
 4681 GTTGACGAAT CTTGGAGCTC CCGCTGCTTT TAAATATTTT GATACAACAA TTGATCGTAA
 4741 ACGATATACG TCTACAAAAG AAGTTTTAGA TGCCACTCTT ATCCATCAAT CCATCACTGG
 4801 TCTTTATGAA ACACGCATTG ATTTGAGTCA GCTAGGAGGT GACtaactcg agtaaggatc
 4861 tccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
 4921 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tggGCCTTTC
 4981 TGCGTTTATA CCTAGGGAGA CGTCGGCGTT CGGCTGCGGC GAGCGGTATc agctcactca
 5041 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
 5101 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
 5161 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
 5221 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
 5281 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
 5341 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
 5401 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
 5461 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
 5521 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
 5581 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
 5641 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
 5701 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
 5761 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgactagt
 5821 gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag
 5881 atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgtaaactt
 5941 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
 6001 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
 6061 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
 6121 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
 6181 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
 6241 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
 6301 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
 6361 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
 6421 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
 6481 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
 6541 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
 6601 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
 6661 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
 6721 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
 6781 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
 6841 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
 6901 aaataggggt tcCGCGCACA TTTCCCCGAA AA
//

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