pAc5.1B-EGFP

价格:1700元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Addgene
载体名称: pAc5.1B-EGFP
质粒类型: 昆虫细胞表达载体
高拷贝/低拷贝: 高拷贝
启动子: --
克隆方法: 多克隆位点,限制性内切酶
载体大小: 6089 bp
5' 测序引物及序列: Ac5 forward:ACACAAAGCCGCTCCATCAG
3' 测序引物及序列: BGH reverse:TAGAAGGCACAGTCGAGG
载体标签: EGFP
载体抗性: 氨苄青霉素(Ampicillin)
筛选标记: --
备注: 克隆菌株:Dh5 alpha 或 XL1-blue
产品目录号: 21181
稳定性: --
组成型: --
病毒/非病毒: --

载体质粒图谱和多克隆位点信息

pAc5.1B-EGFP载体图谱



载体简介

载体序列

LOCUS       pAc5.1B-EGFP	6089 bp 	DNA	SYN
DEFINITION  pAc5.1B-EGFP
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="pAc5.1B-EGFP"
                     /mol_type="other DNA"
     CDS             726..1454
                     /label="ORF frame 3"
                     /translation="MMPDHRLISASFAERSACAVVVFWNSFNFRTVRFDFSFLAYSKS
                     KVKPKKRDGNTKCGKTVVEGKGCGAAEGRVGRGVAGSVAGRDVTDVGATPLTMCACVY
                     YSCLATRRLFFSFYLALSSAISYACSTHRMLPCPLCVILARNRQLFKQRLVNENAKIN
                     KSTIWLLIAMCVQPTIATKATTQWLSLFHFLFASACEQDGTTGKRNYADKEQTKFWPK
                     NIKAPDTNAFAITIAIFNIVYEAV*"
     CDS             complement(1940..2416)
                     /label="ORF frame 2"
                     /translation="MLSALKSRSKLLPYMGMNCSPIGFSFTENFLRNWVFVLVCVLYI
                     AMKSVLPWVLSVAACDSIWGLLIFDTSAGYLYAVMVTVLLHIFSPTTHCMPKHTYVHV
                     FIVGLLVMSSDIKTLQLNNIHISLRLLLSALQIGYSGTFVQHTSSSRGITSRRKHT*"
     misc_feature    2525..2544
                     /label="AC5_primer"
                     /translation="MLSALKSRSKLLPYMGMNCSPIGFSFTENFLRNWVFVLVCVLYI
                     AMKSVLPWVLSVAACDSIWGLLIFDTSAGYLYAVMVTVLLHIFSPTTHCMPKHTYVHV
                     FIVGLLVMSSDIKTLQLNNIHISLRLLLSALQIGYSGTFVQHTSSSRGITSRRKHT*"
     CDS             2588..3445
                     /label="ORF frame 2"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKPSLNSADIQHSGGRSSLEGPRFEGKPIPNPLLGLDST
                     RTGHHHHHH*"
     gene            2591..3304
                     /label="EGFP"
                     /gene="EGFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKPSLNSADIQHSGGRSSLEGPRFEGKPIPNPLLGLDST
                     RTGHHHHHH*"
     misc_feature    complement(2633..2654)
                     /label="EGFP_N_primer"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKPSLNSADIQHSGGRSSLEGPRFEGKPIPNPLLGLDST
                     RTGHHHHHH*"
     misc_feature    2771..2800
                     /label="Y66 (EGFP)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKPSLNSADIQHSGGRSSLEGPRFEGKPIPNPLLGLDST
                     RTGHHHHHH*"
     misc_feature    3241..3262
                     /label="EGFP_C_primer"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKPSLNSADIQHSGGRSSLEGPRFEGKPIPNPLLGLDST
                     RTGHHHHHH*"
     misc_feature    3374..3415
                     /label="V5"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKPSLNSADIQHSGGRSSLEGPRFEGKPIPNPLLGLDST
                     RTGHHHHHH*"
     misc_feature    3425..3442
                     /label="6xHIS"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKPSLNSADIQHSGGRSSLEGPRFEGKPIPNPLLGLDST
                     RTGHHHHHH*"
     misc_feature    complement(3465..3482)
                     /label="BGH_rev_primer"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKPSLNSADIQHSGGRSSLEGPRFEGKPIPNPLLGLDST
                     RTGHHHHHH*"
     terminator      complement(3525..3644)
                     /label="SV40_PA_terminator"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKPSLNSADIQHSGGRSSLEGPRFEGKPIPNPLLGLDST
                     RTGHHHHHH*"
     misc_feature    complement(3539..3558)
                     /label="EBV_rev_primer"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYKPSLNSADIQHSGGRSSLEGPRFEGKPIPNPLLGLDST
                     RTGHHHHHH*"
     CDS             complement(3672..4301)
                     /label="ORF frame 1"
                     /translation="MLVRGAEPMEKRQQRGLFTVPGLLLAGKSRERSLKLSLMVVIYL
                     PGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQRVGRHAGDNGLLLAETFGGG
                     TSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPERCRHLSYELHDKEDSHKCGDD
                     SHAPRPPEGADWVEGSQGHRSTDHNQPYHICRGFTCFKKPPTPPPEPET*"
     CDS             3750..4352
                     /label="ORF frame 3"
                     /translation="MISRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPA
                     ALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADA
                     LGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSQ
                     QKARNRKKAALLAFFHRLRPPDEHHKNRRSSQRWRNPTGL*"
     rep_origin      complement(4256..4875)
                     /label="pBR322_origin"
                     /translation="MISRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPA
                     ALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADA
                     LGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSQ
                     QKARNRKKAALLAFFHRLRPPDEHHKNRRSSQRWRNPTGL*"
     gene            complement(5030..5890)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MISRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPA
                     ALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADA
                     LGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSQ
                     QKARNRKKAALLAFFHRLRPPDEHHKNRRSSQRWRNPTGL*"
     CDS             complement(5030..5890)
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(5932..5960)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
   61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
  121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
  181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
  241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
  301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
  361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
  421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
  481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
  541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
  601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
  661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
  721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
  781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
  841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
  901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
  961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
 1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
 1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
 1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
 1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
 1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
 1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
 1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
 1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
 1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
 1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
 1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
 1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
 1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
 1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
 1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
 1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
 1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
 2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
 2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
 2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
 2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
 2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
 2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
 2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
 2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
 2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
 2581 taccaacatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
 2641 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
 2701 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
 2761 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
 2821 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
 2881 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
 2941 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
 3001 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
 3061 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
 3121 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
 3181 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
 3241 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
 3301 caagccaagc ttgaattctg cagatatcca gcacagtggc ggccgctcga gtctagaggg
 3361 cccgcggttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac
 3421 cggtcatcat caccatcacc attgagttta aacccgctga tcagcctcga ctgtgccttc
 3481 taaggcctga gctcgctgat cagcctcgat cgaggatcca gacatgataa gatacattga
 3541 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
 3601 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
 3661 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
 3721 aaacctctac aaatgtggta tggctgatta tgatcagtcg accgatgccc ttgagagcct
 3781 tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga
 3841 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg
 3901 gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa
 3961 tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga
 4021 agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg
 4081 cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga
 4141 tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc
 4201 aaggatcgct cgcggctctt accagccagc aaaaggccag gaaccgtaaa aaggccgcgt
 4261 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
 4321 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
 4381 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
 4441 cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg
 4501 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
 4561 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
 4621 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
 4681 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
 4741 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
 4801 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
 4861 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
 4921 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
 4981 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
 5041 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
 5101 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
 5161 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
 5221 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
 5281 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
 5341 ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
 5401 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
 5461 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
 5521 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
 5581 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
 5641 cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
 5701 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
 5761 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
 5821 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
 5881 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
 5941 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
 6001 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
 6061 aaaataggcg tatcacgagg ccctttcgt
//

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