| 出品公司: | Biofeng |
|---|---|
| 载体名称: | pcDNA3.1-EGFP, pcDNA3.1(+)EGFP |
| 质粒类型: | 哺乳动物细胞表达载体 |
| 启动子: | CMV |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6119 bp |
| 5' 测序引物: | pcDNA3.1-F:CTAGAGAACCCACTGCTTAC |
| 3' 测序引物: | pcDNA3.1-R (BGH-R):TAGAAGGCACAGTCGAGG |
| 载体标签: | EGFP |
| 载体抗性: | Ampicillin |
| 筛选标记: | Neomycin/G418 |
| 备注: |
EGFP were inserted into pcDNA3.1(+) by BamHI and Not I.
|
| 产品目录号: | -- |
| 稳定性: | 瞬表达 Transient |
| 组成型: | 组成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pcDNA3.1(+)-EGFP 6119 bp DNA circular SYN 21-JUN-2012 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT VNTAUTHORNAME|biofeng.com| FEATURES Location/Qualifiers CDS complement(5123..5983) /invitrogen="1020000" /vntifkey="4" /label=Amp(R) polyA_site 1719..1943 /invitrogen="1870000" /vntifkey="26" /label=BGH\pA primer complement(1713..1730) /invitrogen="1910000" /vntifkey="27" /label=BGH\reverse\primer promoter complement(5984..6082) /invitrogen="2330000" /vntifkey="30" /label=bla\promoter primer 769..789 /invitrogen="1930000" /vntifkey="27" /label=CMV\forward\primer promoter 232..819 /invitrogen="2180000" /vntifkey="29" /label=CMV\promoter rep_origin 1989..2417 /invitrogen="2500000" /vntifkey="33" /label=f1\origin CDS 2827..3621 /invitrogen="1260000" /vntifkey="4" /label=Neo(R) rep_origin complement(4308..4978) /invitrogen="2540000" /vntifkey="33" /label=pUC\origin promoter 2422..2791 /invitrogen="2290000" /vntifkey="29" /label=SV40\early\promoter polyA_signal 3795..3925 /invitrogen="1840000" /vntifkey="25" /label=SV40\pA primer 863..882 /invitrogen="2100000" /vntifkey="27" /label=T7\primer promoter 863..879 /invitrogen="2370000" /vntifkey="30" /label=T7\promoter misc_feature 948..1667 /vntifkey="21" /label=EGFP misc_feature 942..947 /vntifkey="21" /label=Kozak\Sequence BASE COUNT 1417 a 1649 c 1580 g 1473 t ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccaccggt cgccaccatg gtgagcaagg 961 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg 1021 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc 1081 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc 1141 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct 1201 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg 1261 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg 1321 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca 1381 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga 1441 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc 1501 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc 1561 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg 1621 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaagc ggccgctcga 1681 gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc 1741 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt 1801 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct 1861 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc 1921 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg 1981 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag 2041 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt 2101 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt 2161 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg 2221 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt 2281 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt 2341 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca 2401 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca 2461 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt 2521 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 2581 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc 2641 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct 2701 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa 2761 aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg 2821 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg 2881 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg 2941 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat 3001 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca 3061 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg 3121 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat 3181 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa 3241 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg 3301 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg 3361 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg 3421 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat 3481 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac 3541 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc 3601 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc 3661 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg 3721 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt 3781 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca 3841 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac 3901 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat 3961 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 4021 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa 4081 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 4141 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 4201 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 4261 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 4321 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 4381 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 4441 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 4501 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 4561 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 4621 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 4681 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 4741 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 4801 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 4861 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggttttttt gtttgcaagc 4921 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 4981 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 5041 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 5101 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 5161 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 5221 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 5281 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 5341 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 5401 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 5461 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 5521 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 5581 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 5641 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 5701 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 5761 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 5821 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 5881 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 5941 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 6001 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 6061 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc //
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