pgRNA-bacteria

价格:1000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Addgene
载体名称: pgRNA-bacteria
质粒类型: CRISPR/Cas9载体;细菌基因敲除载体
高拷贝/低拷贝: 高拷贝
插入位点: 限制性内切酶,多克隆位点
启动子: J23119(SpeI)
载体大小: 2584 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: mRFP (N-ter)
载体抗性: Ampicillin
克隆菌株: Top10
宿主细胞(系): 细菌,如大肠杆菌
备注:  
产品目录号: #44251
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pgRNA-bacteria 载体图谱



pgRNA-bacteria 多克隆位点

载体简介

载体序列

LOCUS       pgRNA-bacteria	2584 bp 	DNA	SYN
DEFINITION  pgRNA-bacteria
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="pgRNA-bacteria"
                     /mol_type="other DNA"
     misc_feature    complement(285..302)
                     /label="pTrcHis_rev_primer"
     misc_feature    complement(285..302)
                     /label="pBAD_rev_primer"
     terminator      335..492
                     /label="rrnB_terminator"
     terminator      458..501
                     /label="rrnB_T1_terminator"
     gene            complement(1484..2344)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(1484..2344)
                     /label="ORF frame 1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(2386..2414)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 gAATTCTAAA GATCTTTGAC AGCTAGCTCA GTCCTAGGTA TAATACTAGT AACTTTCAGT
   61 TTAGCGGTCT GTTTTAGAGC TAGAAATAGC AAGTTAAAAT AAGGCTAGTC CGTTATCAAC
  121 TTGAAAAAGT GGCACCGAGT CGGTGCTTTT TTTGAAGCTT GGGCCCGAAC AAAAACTCat
  181 ctcagaagag gatctgaata gcgccgtcga ccatcatcat catcatcatt gagtttaaac
  241 ggtctccagc ttggctgttt tggcggatga gagaagattt tcagcctgat acagattaaa
  301 tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc
  361 ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg
  421 tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa
  481 agactGGGCC TTTCGTTTTA TCTGTTGTTT GTCGGTGAAC TGGATCCTTA CTCGAGTCTA
  541 GACTGCAGgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
  601 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
  661 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
  721 cgtttttcca Caggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
  781 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
  841 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
  901 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
  961 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
 1021 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
 1081 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
 1141 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
 1201 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
 1261 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
 1321 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
 1381 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
 1441 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
 1501 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
 1561 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
 1621 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
 1681 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
 1741 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
 1801 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
 1861 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
 1921 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
 1981 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
 2041 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
 2101 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
 2161 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
 2221 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
 2281 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
 2341 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
 2401 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
 2461 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
 2521 ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt
 2581 tctg
//

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