| 出品公司: | -- |
|---|---|
| 载体名称: | pCBC-DT1T2(Cm) 载体 |
| 质粒类型: | 植物表达载体,CRISPR/CAS9系统 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | -- |
| 载体大小: | 3525 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氯霉素 |
| 筛选标记: | 嘌呤霉素（Puromycin） |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: | |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
 载体载体图谱.png)
载体简介
载体序列
LOCUS pCBC-DT1T2(Cm) 3525 bp DNA circular SYN 13-JUL-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3525)
AUTHORS Biofeng
TITLE Direct Submission
JOURNAL Exported Jul 13, 2023 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..3525
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_RNA 1..76
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
gap 555..573
/estimated_length=19
promoter complement(625..643)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(661..677)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(716..763)
/label=EM7 promoter
/note="synthetic bacterial promoter "
terminator complement(805..832)
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator complement(924..1010)
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
rep_origin complement(1175..1763)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1988..2647)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKSKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
CDS complement(2996..3298)
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 3436..3452
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 3459..3477
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
61 ggcaccgagt cggtgctttt ttttgcaaaa ttttccagat cgatttcttc ttcctctgtt
121 cttcggcgtt caatttctgg ggttttctct tcgttttctg taactgaaac ctaaaatttg
181 acctaaaaaa aatctcaaat aatatgattc agtggttttg tacttttcag ttagttgagt
241 tttgcagttc cgatgagata aaccaatatt aatccaaact actgcagcct gacagacaaa
301 tgaggatgca aacaatttta aagtttatct aacgctagct gttttgtttc ttctctctgg
361 tgcaccaacg acggcgtttt ctcaatcata aagaggcttg ttttacttaa ggccaataat
421 gttgatggat cgaaagaaga gggcttttaa taaacgagcc cgtttaagct gtaaacgatg
481 tcaaaaacat cccacatcgt tcagttgaaa atagaagctc tgtttatata ttggtagagt
541 cgactaagag attgnnnnnn nnnnnnnnnn nnngtttaga gaccaataat ccttggcctt
601 aagggccttg catagcttga gtattctata gtgtcaccta aatagcttgg cgtaatcatg
661 gtcatagctg tttcctgtgt gaaattgtta tccgctcggt ttagttcctc accttgtcgt
721 attatactat gccgatatac tatgccgatg attaattgtc aacacttaag taactaacta
781 acaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt
841 tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca
901 acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa
961 cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca
1021 gttccctact ctcgcttagt agttagacgt gcccgagatc catcctagcg gtaatacggt
1081 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
1141 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
1201 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
1261 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
1321 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
1381 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
1441 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
1501 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
1561 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
1621 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
1681 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
1741 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctaccggg tctgacgctc
1801 agtggaacgg ggcgggcggc gttgtgacaa tttaccgaac aactccgcgg gacttgacct
1861 gatagtttgg ctgtgagcaa ttatgtgctt agtgcatcta acgcttgagt taagccgcgc
1921 cgcgaagcgg cgtcggcttg aacgaatttc tagctagagg atcgcaccaa taactgcctt
1981 aaaaaaatta cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc
2041 tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca
2101 ccttgtcgcc ttgcgtataa tatttgccca ttgtgaaaac gggggcgaag aagttgtcca
2161 tattggccac gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa
2221 acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat
2281 cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg
2341 aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca
2401 ccagctcacc gtctttcatt gccatacgga actccggatg agcattcatc aggcgggcaa
2461 gaatgtgaat aaaggccgga taaaacttgt gcttactttt ctttacggtc tttaaaaagg
2521 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
2581 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
2641 tctccatgat gtttaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc
2701 tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca
2761 tagactgtac cccaaaaaaa cagtcataac aagccatgaa aaccgccact gcgccgttac
2821 caccgctgcg ttcggtcaag gttctggacc agttgcgtga gcgcatacgc tacttgcatt
2881 acagcttacg aaccgaacga ggcttatgtc cactcatggt ccgtttctgc ggactggctt
2941 tctacgtgaa aaggatctag gtgaagatcc tttttgataa tctcatgcct gacatttata
3001 ttccccagaa catcaggtta atggcgtttt tgatgtcatt ttcgcggtgg ctgagatcag
3061 ccacttcttc cccgataacg gatactggca cacttgccat atcggtggtc atcatgcgcc
3121 agctttcatc cccgatatgc accaccgggt aaagttcacg ggagacttta tctgacagca
3181 gacgtgcact ggccaggggg atcaccatcc gtcgccccgg cgtgtcaata atatcactct
3241 gtacatccac aaacagacga taacggctct ctcttttata ggtgtaaacc ttaaactgcc
3301 gtacgtatag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
3361 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
3421 cccagtcacg acgttgtaaa acgacggcca gtgaattgta atacgactca ctatagggcg
3481 aattgggcca ggccaaatag gccaaaggaa taatggtctc tattg
//
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