pWJ66

价格:1000元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Addgene
载体名称: pwtCas9-bacteria
质粒类型: CRISPR/Cas9载体;细菌基因敲除载体
高拷贝/低拷贝: 低拷贝
插入位点: 限制性内切酶,多克隆位点
启动子: --
载体大小: 9552 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: rpoZ (C ter)
载体抗性: Chloramphenicol
克隆菌株: Top10
宿主细胞(系): 细菌,如大肠杆菌
备注:  
产品目录号: #46570
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

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载体质粒图谱和多克隆位点信息

pWJ66载体图谱

载体简介

载体序列

LOCUS       pWJ66	9552 bp 	DNA	SYN
DEFINITION  pWJ66
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..9552
                     /organism="pWJ66"
                     /mol_type="other DNA"
     gene            complement(4..219)
                     /label="CAT/CamR (659 - 444)"
                     /gene="CAT/CamR (659 - 444)"
     rep_origin      581..1493
                     /label="p15A_origin"
     CDS             2225..6607
                     /label="ORF frame 2"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIK
                     KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
                     ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH
                     MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK
                     SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD
                     NLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTL
                     LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK
                     LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
                     YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL
                     PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE
                     DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT
                     LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF
                     LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT
                     VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE
                     HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKV
                     LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA
                     GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG
                     KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
                     MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
                     AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF
                     ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH
                     KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA
                     PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDAAARVTVQDA
                     VEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDKTTVIALREIEEGLINNQILDVR
                     ERQEQQEQEAAELQAVTAIAEGRR*"
     gene            6996..8078
                     /label="tet (variant)"
                     /gene="tet (variant)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIK
                     KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
                     ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH
                     MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK
                     SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD
                     NLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTL
                     LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVK
                     LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
                     YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL
                     PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE
                     DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT
                     LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF
                     LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT
                     VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE
                     HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKV
                     LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA
                     GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG
                     KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
                     MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
                     AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF
                     ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH
                     KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA
                     PAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDAAARVTVQDA
                     VEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDKTTVIALREIEEGLINNQILDVR
                     ERQEQQEQEAAELQAVTAIAEGRR*"
     CDS             7050..8078
                     /label="ORF frame 3"
                     /translation="MQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWI
                     LYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGA
                     ISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAA
                     LMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATK
                     RFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDD
                     DHQGQLQGSLAALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRG
                     AWSRATST*"
     misc_feature    complement(7240..7259)
                     /label="pBRrevBam_primer"
                     /translation="MQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWI
                     LYAGRIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGA
                     ISLHAPFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAA
                     LMTVFFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATK
                     RFGEKQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDD
                     DHQGQLQGSLAALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRG
                     AWSRATST*"
     CDS             complement(7425..7883)
                     /label="ORF frame 2"
                     /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ
                     RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER
                     CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSTLSLMRLLH*"
     gene            complement(9112..219)
                     /label="CAT/CamR"
                     /gene="CAT/CamR"
                     /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ
                     RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER
                     CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSTLSLMRLLH*"
     CDS             complement(9112..219)
                     /label="ORF frame 1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
ORIGIN
    1 GAATTCCGGA TGAGCATTCA TCAGGCGGGC AAGAATGTGA ATAAAGGCCG GATAAAACTT
   61 GTGCTTATTT TTCTTTACGG TCTTTAAAAA GGCCGTAATA TCCAGCTGAA CGGTCTGGTT
  121 ATAGGTACAT TGAGCAACTG ACTGAAATGC CTCAAAATGT TCTTTACGAT GCCATTGGGA
  181 TATATCAACG GTGGTATATC CAGTGATTTT TTTCTCCATT TTAGCTTCCT TAGCTCCTGA
  241 AAATCTCGAT AACTCAAAAA ATACGCCCGG TAGTGATCTT ATTTCATTAT GGTGAAAGTT
  301 GGAACCTCTT ACGTGCCGAT CAACGTCTCA TTTTCGCCAA AAGTTGGCCC AGGGCTTCCC
  361 GGTATCAACA GGGACACCAG GATTTATTTA TTCTGCGAAG TGATCTTCCG TCACAGGTAT
  421 TTATTCGGCG CAAAGTGCGT CGGGTGATGC TGCCAACTTA CTGATTTAGT GTATGATGGT
  481 GTTTTTGAGG TGCTCCAGTG GCTTCTGTTT CTATCAGCTG TCCCTCCTGT TCAGCTACTG
  541 ACGGGGTGGT GCGTAACGGC AAAAGCACCG CCGGACATCA GCGCTAGCGG AGTGTATACT
  601 GGCTTACTAT GTTGGCACTG ATGAGGGTGT CAGTGAAGTG CTTCATGTGG CAGGAGAAAA
  661 AAGGCTGCAC CGGTGCGTCA GCAGAATATG TGATACAGGA TATATTCCGC TTCCTCGCTC
  721 ACTGACTCGC TACGCTCGGT CGTTCGACTG CGGCGAGCGG AAATGGCTTA CGAACGGGGC
  781 GGAGATTTCC TGGAAGATGC CAGGAAGATA CTTAACAGGG AAGTGAGAGG GCCGCGGCAA
  841 AGCCGTTTTT CCATAGGCTC CGCCCCCCTG ACAAGCATCA CGAAATCTGA CGCTCAAATC
  901 AGTGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGCGGCTCCC
  961 TCGTGCGCTC TCCTGTTCCT GCCTTTCGGT TTACCGGTGT CATTCCGCTG TTATGGCCGC
 1021 GTTTGTCTCA TTCCACGCCT GACACTCAGT TCCGGGTAGG CAGTTCGCTC CAAGCTGGAC
 1081 TGTATGCACG AACCCCCCGT TCAGTCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT
 1141 GAGTCCAACC CGGAAAGACA TGCAAAAGCA CCACTGGCAG CAGCCACTGG TAATTGATTT
 1201 AGAGGAGTTA GTCTTGAAGT CATGCGCCGG TTAAGGCTAA ACTGAAAGGA CAAGTTTTGG
 1261 TGACTGCGCT CCTCCAAGCC AGTTACCTCG GTTCAAAGAG TTGGTAGCTC AGAGAACCTT
 1321 CGAAAAACCG CCCTGCAAGG CGGTTTTTTC GTTTTCAGAG CAAGAGATTA CGCGCAGACC
 1381 AAAACGATCT CAAGAAGATC ATCTTATTAA TCAGATAAAA TATTTCTAGA TTTCAGTGCA
 1441 ATTTATCTCT TCAAATGTAG CACCTGAAGT CAGCCCCATA CGATATAAGT TGTAATTCTC
 1501 ATGTTTGACA GCTTATCATC GATAAGCTTT AATGCGGTAG TTTATCACAG TTAAATTGCT
 1561 AACGCAGTCA GGCACCGTGT ATGAAATCTA ACAATGCGCT CATCGTCATC CTCGGCACCG
 1621 TCACCCTGGA TGCTGTAGGC ATAGGCTTGG TTATGCCGGT ACTGCCGGGC CTCTTGCGGG
 1681 ATTACGAAAT CATCCTGTGG AGCTTAGTAG GTTTAGCAAG ATGGCAGCGC CTAAATGTAG
 1741 AATGATAAAA GGATTAAGAG ATTAATTTCC CTAAAAATGA TAAAACAAGC GTTTTGAAAG
 1801 CGCTTGTTTT TTTGGTTTGC AGTCAGAGTA GAATAGAAGT ATCAAAAAAA GCACCGACTC
 1861 GGTGCCACTT TTTCAAGTTG ATAACGGACT AGCCTTATTT TAACTTGCTA TGCTGTTTTG
 1921 AATGGTTCCA ACAAGATTAT TTTATAACTT TTATAACAAA TAATCAAGGA GAAATTCAAA
 1981 GAAATTTATC AGCCATAAAA CAATACTTAA TACTATAGAA TGATAACAAA ATAAACTACT
 2041 TTTTAAAAGA ATTTTGTGTT ATAATCTATT TATTATTAAG TATTGGGTAA TATTTTTTGA
 2101 AGAGATATTT TGAAAAAGAA AAATTAAAGC ATATTAAACT AATTTCGGAG GTCATTAAAA
 2161 CTATTATTGA AATCATCAAA CTCATTATGG ATTTAATTTA AACTTTTTAT TTTAGGAGGC
 2221 AAAAATGGAT AAGAAATACT CAATAGGCTT AGCTATCGGC ACAAATAGCG TCGGATGGGC
 2281 GGTGATCACT GATGAATATA AGGTTCCGTC TAAAAAGTTC AAGGTTCTGG GAAATACAGA
 2341 CCGCCACAGT ATCAAAAAAA ATCTTATAGG GGCTCTTTTA TTTGACAGTG GAGAGACAGC
 2401 GGAAGCGACT CGTCTCAAAC GGACAGCTCG TAGAAGGTAT ACACGTCGGA AGAATCGTAT
 2461 TTGTTATCTA CAGGAGATTT TTTCAAATGA GATGGCGAAA GTAGATGATA GTTTCTTTCA
 2521 TCGACTTGAA GAGTCTTTTT TGGTGGAAGA AGACAAGAAG CATGAACGTC ATCCTATTTT
 2581 TGGAAATATA GTAGATGAAG TTGCTTATCA TGAGAAATAT CCAACTATCT ATCATCTGCG
 2641 AAAAAAATTG GTAGATTCTA CTGATAAAGC GGATTTGCGC TTAATCTATT TGGCCTTAGC
 2701 GCATATGATT AAGTTTCGTG GTCATTTTTT GATTGAGGGA GATTTAAATC CTGATAATAG
 2761 TGATGTGGAC AAACTATTTA TCCAGTTGGT ACAAACCTAC AATCAATTAT TTGAAGAAAA
 2821 CCCTATTAAC GCAAGTGGAG TAGATGCTAA AGCGATTCTT TCTGCACGAT TGAGTAAATC
 2881 AAGACGATTA GAAAATCTCA TTGCTCAGCT CCCCGGTGAG AAGAAAAATG GCTTATTTGG
 2941 GAATCTCATT GCTTTGTCAT TGGGTTTGAC CCCTAATTTT AAATCAAATT TTGATTTGGC
 3001 AGAAGATGCT AAATTACAGC TTTCAAAAGA TACTTACGAT GATGATTTAG ATAATTTATT
 3061 GGCGCAAATT GGAGATCAAT ATGCTGATTT GTTTTTGGCA GCTAAGAATT TATCAGATGC
 3121 TATTTTACTT TCAGATATCC TAAGAGTAAA TACTGAAATA ACTAAGGCTC CCCTATCAGC
 3181 TTCAATGATT AAACGCTACG ATGAACATCA TCAAGACTTG ACTCTTTTAA AAGCTTTAGT
 3241 TCGACAACAA CTTCCAGAAA AGTATAAAGA AATCTTTTTT GATCAATCAA AAAACGGATA
 3301 TGCAGGTTAT ATTGATGGGG GAGCTAGCCA AGAAGAATTT TATAAATTTA TCAAACCAAT
 3361 TTTAGAAAAA ATGGATGGTA CTGAGGAATT ATTGGTGAAA CTAAATCGTG AAGATTTGCT
 3421 GCGCAAGCAA CGGACCTTTG ACAACGGCTC TATTCCCCAT CAAATTCACT TGGGTGAGCT
 3481 GCATGCTATT TTGAGAAGAC AAGAAGACTT TTATCCATTT TTAAAAGACA ATCGTGAGAA
 3541 GATTGAAAAA ATCTTGACTT TTCGAATTCC TTATTATGTT GGTCCATTGG CGCGTGGCAA
 3601 TAGTCGTTTT GCATGGATGA CTCGGAAGTC TGAAGAAACA ATTACCCCAT GGAATTTTGA
 3661 AGAAGTTGTC GATAAAGGTG CTTCAGCTCA ATCATTTATT GAACGCATGA CAAACTTTGA
 3721 TAAAAATCTT CCAAATGAAA AAGTACTACC AAAACATAGT TTGCTTTATG AGTATTTTAC
 3781 GGTTTATAAC GAATTGACAA AGGTCAAATA TGTTACTGAA GGAATGCGAA AACCAGCATT
 3841 TCTTTCAGGT GAACAGAAGA AAGCCATTGT TGATTTACTC TTCAAAACAA ATCGAAAAGT
 3901 AACCGTTAAG CAATTAAAAG AAGATTATTT CAAAAAAATA GAATGTTTTG ATAGTGTTGA
 3961 AATTTCAGGA GTTGAAGATA GATTTAATGC TTCATTAGGT ACCTACCATG ATTTGCTAAA
 4021 AATTATTAAA GATAAAGATT TTTTGGATAA TGAAGAAAAT GAAGATATCT TAGAGGATAT
 4081 TGTTTTAACA TTGACCTTAT TTGAAGATAG GGAGATGATT GAGGAAAGAC TTAAAACATA
 4141 TGCTCACCTC TTTGATGATA AGGTGATGAA ACAGCTTAAA CGTCGCCGTT ATACTGGTTG
 4201 GGGACGTTTG TCTCGAAAAT TGATTAATGG TATTAGGGAT AAGCAATCTG GCAAAACAAT
 4261 ATTAGATTTT TTGAAATCAG ATGGTTTTGC CAATCGCAAT TTTATGCAGC TGATCCATGA
 4321 TGATAGTTTG ACATTTAAAG AAGACATTCA AAAAGCACAA GTGTCTGGAC AAGGCGATAG
 4381 TTTACATGAA CATATTGCAA ATTTAGCTGG TAGCCCTGCT ATTAAAAAAG GTATTTTACA
 4441 GACTGTAAAA GTTGTTGATG AATTGGTCAA AGTAATGGGG CGGCATAAGC CAGAAAATAT
 4501 CGTTATTGAA ATGGCACGTG AAAATCAGAC AACTCAAAAG GGCCAGAAAA ATTCGCGAGA
 4561 GCGTATGAAA CGAATCGAAG AAGGTATCAA AGAATTAGGA AGTCAGATTC TTAAAGAGCA
 4621 TCCTGTTGAA AATACTCAAT TGCAAAATGA AAAGCTCTAT CTCTATTATC TCCAAAATGG
 4681 AAGAGACATG TATGTGGACC AAGAATTAGA TATTAATCGT TTAAGTGATT ATGATGTCGA
 4741 TGCCATTGTT CCACAAAGTT TCCTTAAAGA CGATTCAATA GACAATAAGG TCTTAACGCG
 4801 TTCTGATAAA AATCGTGGTA AATCGGATAA CGTTCCAAGT GAAGAAGTAG TCAAAAAGAT
 4861 GAAAAACTAT TGGAGACAAC TTCTAAACGC CAAGTTAATC ACTCAACGTA AGTTTGATAA
 4921 TTTAACGAAA GCTGAACGTG GAGGTTTGAG TGAACTTGAT AAAGCTGGTT TTATCAAACG
 4981 CCAATTGGTT GAAACTCGCC AAATCACTAA GCATGTGGCA CAAATTTTGG ATAGTCGCAT
 5041 GAATACTAAA TACGATGAAA ATGATAAACT TATTCGAGAG GTTAAAGTGA TTACCTTAAA
 5101 ATCTAAATTA GTTTCTGACT TCCGAAAAGA TTTCCAATTC TATAAAGTAC GTGAGATTAA
 5161 CAATTACCAT CATGCCCATG ATGCGTATCT AAATGCCGTC GTTGGAACTG CTTTGATTAA
 5221 GAAATATCCA AAACTTGAAT CGGAGTTTGT CTATGGTGAT TATAAAGTTT ATGATGTTCG
 5281 TAAAATGATT GCTAAGTCTG AGCAAGAAAT AGGCAAAGCA ACCGCAAAAT ATTTCTTTTA
 5341 CTCTAATATC ATGAACTTCT TCAAAACAGA AATTACACTT GCAAATGGAG AGATTCGCAA
 5401 ACGCCCTCTA ATCGAAACTA ATGGGGAAAC TGGAGAAATT GTCTGGGATA AAGGGCGAGA
 5461 TTTTGCCACA GTGCGCAAAG TATTGTCCAT GCCCCAAGTC AATATTGTCA AGAAAACAGA
 5521 AGTACAGACA GGCGGATTCT CCAAGGAGTC AATTTTACCA AAAAGAAATT CGGACAAGCT
 5581 TATTGCTCGT AAAAAAGACT GGGATCCAAA AAAATATGGT GGTTTTGATA GTCCAACGGT
 5641 AGCTTATTCA GTCCTAGTGG TTGCTAAGGT GGAAAAAGGG AAATCGAAGA AGTTAAAATC
 5701 CGTTAAAGAG TTACTAGGGA TCACAATTAT GGAAAGAAGT TCCTTTGAAA AAAATCCGAT
 5761 TGACTTTTTA GAAGCTAAAG GATATAAGGA AGTTAAAAAA GACTTAATCA TTAAACTACC
 5821 TAAATATAGT CTTTTTGAGT TAGAAAACGG TCGTAAACGG ATGCTGGCTA GTGCCGGAGA
 5881 ATTACAAAAA GGAAATGAGC TGGCTCTGCC AAGCAAATAT GTGAATTTTT TATATTTAGC
 5941 TAGTCATTAT GAAAAGTTGA AGGGTAGTCC AGAAGATAAC GAACAAAAAC AATTGTTTGT
 6001 GGAGCAGCAT AAGCATTATT TAGATGAGAT TATTGAGCAA ATCAGTGAAT TTTCTAAGCG
 6061 TGTTATTTTA GCAGATGCCA ATTTAGATAA AGTTCTTAGT GCATATAACA AACATAGAGA
 6121 CAAACCAATA CGTGAACAAG CAGAAAATAT TATTCATTTA TTTACGTTGA CGAATCTTGG
 6181 AGCTCCCGCT GCTTTTAAAT ATTTTGATAC AACAATTGAT CGTAAACGAT ATACGTCTAC
 6241 AAAAGAAGTT TTAGATGCCA CTCTTATCCA TCAATCCATC ACTGGTCTTT ATGAAACACG
 6301 CATTGATTTG AGTCAGCTAG GAGGTGACGC AGCTGCACGC GTAACTGTTC AGGACGCTGT
 6361 AGAGAAAATT GGTAACCGTT TTGACCTGGT ACTGGTCGCC GCGCGTCGCG CTCGTCAGAT
 6421 GCAGGTAGGC GGAAAGGATC CGCTGGTACC GGAAGAAAAC GATAAAACCA CTGTAATCGC
 6481 GCTGCGCGAA ATCGAAGAAG GTCTGATCAA CAACCAGATC CTCGACGTTC GCGAACGCCA
 6541 GGAACAGCAA GAGCAGGAAG CCGCTGAATT ACAAGCCGTT ACCGCTATTG CTGAAGGTCG
 6601 TCGTTAATAC TCTTAATAAA TGCAGTAATA CAGGGGCTTT TCAAGACTGA AGTCTAGCTG
 6661 AGACAAATAG TGCGATTACG AAATTTTTTA GACAAAAATA GTCTACGAGG TTTTAGAGCT
 6721 ATGCTGTTTT GAATGGTCCC AAAACTGAGA CCAGTCTCGG AAGCTCAAAG GTCTCGTTTT
 6781 AGAGCTATGC TGTTTTGAAT GGTCCCAAAA CTTCAGCACA CTGAGACTTG TTGAGTTCCA
 6841 TGTTTTAGAG CTATGCTGTT TTGAATGGAC TCCATTCAAC ATTGCCGATG ATAACTTGAG
 6901 AAAGAGGGTT AATACCAGCA GTCGGATACC TTCCTATTCT TTCTGTTAAA GCGTTTTCAT
 6961 GTTATAATAG GCAAAAGAAG AGTAGTGTGA TCGTCCATTC CGACAGCATC GCCAGTCACT
 7021 ATGGCGTGCT GCTAGCGCTA TATGCGTTGA TGCAATTTCT ATGCGCACCC GTTCTCGGAG
 7081 CACTGTCCGA CCGCTTTGGC CGCCGCCCAG TCCTGCTCGC TTCGCTACTT GGAGCCACTA
 7141 TCGACTACGC GATCATGGCG ACCACACCCG TCCTGTGGAT CCTCTACGCC GGACGCATCG
 7201 TGGCCGGCAT CACCGGCGCC ACAGGTGCGG TTGCTGGCGC CTATATCGCC GACATCACCG
 7261 ATGGGGAAGA TCGGGCTCGC CACTTCGGGC TCATGAGCGC TTGTTTCGGC GTGGGTATGG
 7321 TGGCAGGCCC CGTGGCCGGG GGACTGTTGG GCGCCATCTC CTTGCATGCA CCATTCCTTG
 7381 CGGCGGCGGT GCTCAACGGC CTCAACCTAC TACTGGGCTG CTTCCTAATG CAGGAGTCGC
 7441 ATAAGGGAGA GCGTCGACCG ATGCCCTTGA GAGCCTTCAA CCCAGTCAGC TCCTTCCGGT
 7501 GGGCGCGGGG CATGACTATC GTCGCCGCAC TTATGACTGT CTTCTTTATC ATGCAACTCG
 7561 TAGGACAGGT GCCGGCAGCG CTCTGGGTCA TTTTCGGCGA GGACCGCTTT CGCTGGAGCG
 7621 CGACGATGAT CGGCCTGTCG CTTGCGGTAT TCGGAATCTT GCACGCCCTC GCTCAAGCCT
 7681 TCGTCACTGG TCCCGCCACC AAACGTTTCG GCGAGAAGCA GGCCATTATC GCCGGCATGG
 7741 CGGCCGACGC GCTGGGCTAC GTCTTGCTGG CGTTCGCGAC GCGAGGCTGG ATGGCCTTCC
 7801 CCATTATGAT TCTTCTCGCT TCCGGCGGCA TCGGGATGCC CGCGTTGCAG GCCATGCTGT
 7861 CCAGGCAGGT AGATGACGAC CATCAGGGAC AGCTTCAAGG ATCGCTCGCG GCTCTTACCA
 7921 GCCTAACTTC GATCATTGGA CCGCTGATCG TCACGGCGAT TTATGCCGCC TCGGCGAGCA
 7981 CATGGAACGG GTTGGCATGG ATTGTAGGCG CCGCCCTATA CCTTGTCTGC CTCCCCGCGT
 8041 TGCGTCGCGG TGCATGGAGC CGGGCCACCT CGACCTGAAT GGAAGCCGGC GGCACCTCGC
 8101 TAACGGATTC ACCACTCCAA GAATTGGAGC CAATCAATTC TTGCGGAGAA CTGTGAATGC
 8161 GCAAACCAAC CCTTGGCAGA ACATATCCAT CGCGTCCGCC ATCTCCAGCA GCCGCACGCG
 8221 GCGCATCTCG GGCAGCGTTG GGTCCTGGCC ACGGGTGCGC ATGATCGTGC TCCTGTCGTT
 8281 GAGGACCCGG CTAGGCTGGC GGGGTTGCCT TACTGGTTAG CAGAATGAAT CACCGATACG
 8341 CGAGCGAACG TGAAGCGACT GCTGCTGCAA AACGTCTGCG ACCTGAGCAA CAACATGAAT
 8401 GGTCTTCGGT TTCCGTGTTT CGTAAAGTCT GGAAACGCGG AAGTCCCCTA CGTGCTGCTG
 8461 AAGTTGCCCG CAACAGAGAG TGGAACCAAC CGGTGATACC ACGATACTAT GACTGAGAGT
 8521 CAACGCCATG AGCGGCCTCA TTTCTTATTC TGAGTTACAA CAGTCCGCAC CGCTGTCCGG
 8581 TAGCTCCTTC CGGTGGGCGC GGGGCATGAC TATCGTCGCC GCACTTATGA CTGTCTTCTT
 8641 TATCATGCAA CTCGTAGGAC AGGTGCCGGC AGCGCCCAAC AGTCCCCCGG CCACGGGGCC
 8701 TGCCACCATA CCCACGCCGA AACAAGCGCC CTGCACCATT ATGTTCCGGA TCTGCATCGC
 8761 AGGATGCTGC TGGCTACCCT GTGGAACACC TACATCTGTA TTAACGAAGC GCTAACCGTT
 8821 TTTATCAGGC TCTGGGAGGC AGAATAAATG ATCATATCGT CAATTATTAC CTCCACGGGG
 8881 AGAGCCTGAG CAAACTGGCC TCAGGCATTT GAGAAGCACA CGGTCACACT GCTTCCGGTA
 8941 GTCAATAAAC CGGTAAACCA GCAATAGACA TAAGCGGCTA TTTAACGACC CTGCCCTGAA
 9001 CCGACGACCG GGTCGAATTT GCTTTCGAAT TTCTGCCATT CATCCGCTTA TTATCACTTA
 9061 TTCAGGCGTA GCACCAGGCG TTTAAGGGCA CCAATAACTG CCTTAAAAAA ATTACGCCCC
 9121 GCCCTGCCAC TCATCGCAGT ACTGTTGTAA TTCATTAAGC ATTCTGCCGA CATGGAAGCC
 9181 ATCACAGACG GCATGATGAA CCTGAATCGC CAGCGGCATC AGCACCTTGT CGCCTTGCGT
 9241 ATAATATTTG CCCATGGTGA AAACGGGGGC GAAGAAGTTG TCCATATTGG CCACGTTTAA
 9301 ATCAAAACTG GTGAAACTCA CCCAGGGATT GGCTGAGACG AAAAACATAT TCTCAATAAA
 9361 CCCTTTAGGG AAATAGGCCA GGTTTTCACC GTAACACGCC ACATCTTGCG AATATATGTG
 9421 TAGAAACTGC CGGAAATCGT CGTGGTATTC ACTCCAGAGC GATGAAAACG TTTCAGTTTG
 9481 CTCATGGAAA ACGGTGTAAC AAGGGTGAAC ACTATCCCAT ATCACCAGCT CACCGTCTTT
 9541 CATTGCCATA CG
//

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