| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pSecTag2 B |
| 质粒类型: | 哺乳动物细胞载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | -- |
| 启动子: | -- |
| 载体大小: | 5163 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
pSecTag2 B载体 pSecTag2B质粒基本信息
pSecTag2 B质粒图谱 pSecTag2B载体图谱和pSecTag2 B载体序列 pSecTag2B质粒序列多克隆位点信息
pSecTag2 B质粒 pSecTag2B载体简介
pSecTag2 B质粒序列 pSecTag2B载体序列
LOCUS pSecTag2 B 5163 bp ds-DNA circular SYN 24-OCT-2017
DEFINITION Mammalian expression vector for the secretion of C-terminally
6xHis-tagged proteins. For other reading frames, use pSecTag2 A or
pSecTag2 C.
ACCESSION .
VERSION .
KEYWORDS pSecTag2 B
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5163)
AUTHORS Thermo Fisher (Invitrogen)
TITLE Direct Submission
JOURNAL Exported Tuesday, February 11, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5163
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 235..614
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 863..881
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 905..967
/codon_start=1
/product="leader sequence from mouse immunoglobulin kappa
light chain"
/note="Ig-kappa leader"
/translation="METDTLLLWVLLLWVPGSTGD"
misc_feature 970..1085
/note="MCS"
/note="multiple cloning site"
CDS 1086..1115
/codon_start=1
/product="Myc (human c-Myc oncogene) epitope tag"
/note="Myc"
/translation="EQKLISEEDL"
CDS 1131..1148
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
polyA_signal 1177..1401
/note="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
rep_origin 1447..1875
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1889..2218
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2069..2204
/note="SV40 ori"
/note="SV40 origin of replication"
promoter 2266..2313
/note="EM7 promoter"
/note="synthetic bacterial promoter "
CDS 2332..2706
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/note="BleoR"
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 2836..2957
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(3408..3996)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4167..5027)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5028..5132)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 caccatggag acagacacac tcctgctatg ggtactgctg ctctgggttc caggttccac
961 tggtgacgcg gcccagccgg ccaggcgcgc gcgccgtacg aagcttggta ccgagctcgg
1021 atccactcca gtgtggtgga attctgcaga tatccagcac agtggcggcc gctcgaggag
1081 ggcccgaaca aaaactcatc tcagaagagg atctgaatag cgccgtcgac catcatcatc
1141 atcatcattg agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
1201 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
1261 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
1321 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
1381 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
1441 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
1501 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
1561 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg gggcatccct ttagggttcc
1621 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
1681 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
1741 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
1801 atttataagg gattttgggg atttcggcct attggttaaa aaatgagctg atttaacaaa
1861 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
1921 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
1981 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
2041 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
2101 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
2161 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
2221 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca
2281 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag
2341 ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg
2401 accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg
2461 gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg
2521 gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc
2581 acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg
2641 cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag
2701 gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
2761 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
2821 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
2881 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
2941 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
3001 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
3061 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
3121 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
3181 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
3241 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
3301 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
3361 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
3421 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
3481 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
3541 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
3601 caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
3661 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
3721 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
3781 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
3841 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
3901 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
3961 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
4021 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
4081 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
4141 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
4201 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
4261 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
4321 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
4381 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
4441 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
4501 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
4561 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
4621 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
4681 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
4741 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
4801 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
4861 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
4921 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
4981 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
5041 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
5101 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
5161 gtc
//
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