pGL4.13

价格:1250元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Promega
载体名称: pGL4.13 、 pGL4.13[luc2/SV40]
质粒类型: 哺乳细胞表达载体;信号通路报告载体
高拷贝/低拷贝: --
启动子: SV40
克隆方法: 多克隆位点,限制性内切酶
载体大小: 4641 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素Ampicillin
筛选标记: --
备注: pGL4.13载体含有SV40启动子,能够高水平组成型表达荧光素酶。
产品目录号: E6681
稳定性: --
组成型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pGL4.13载体图谱
pGL4.13载体特征

载体简介

The pGL4.13[luc2/SV40] Vector(a–e) encodes the luciferase reporter gene luc2 (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been codon optimized for mammalian expression.
The pGL4.13[luc2/ SV40] Vector contains the luc2 reporter gene and the SV40 early enhancer/promoter for use as an expression control or a co-reporter vector.

载体序列

LOCUS       pGL4.13[luc2/SV40]	4641 bp 	DNA	SYN
DEFINITION  pGL4.13[luc2/SV40]
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="pGL4.13[luc2/SV40]"
                     /mol_type="other DNA"
     misc_feature    complement(129..149)
                     /label="pBABE_3_primer"
     misc_feature    complement(135..350)
                     /label="SV40_enhancer"
     promoter        147..415
                     /label="SV40_promoter"
     rep_origin      314..391
                     /label="SV40_origin"
     misc_feature    376..395
                     /label="SV40pro_F_primer"
     CDS             499..2151
                     /label="ORF frame 1"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                     AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
                     APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
                     FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
                     FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
                     LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
                     GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
                     RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
                     PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
                     AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
     terminator      complement(2192..2311)
                     /label="SV40_PA_terminator"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                     AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
                     APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
                     FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
                     FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
                     LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
                     GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
                     RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
                     PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
                     AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
     misc_feature    complement(2206..2225)
                     /label="EBV_rev_primer"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                     AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
                     APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
                     FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
                     FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
                     LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
                     GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
                     RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
                     PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
                     AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
     rep_origin      complement(2720..3339)
                     /label="pBR322_origin"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                     AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
                     APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
                     FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
                     FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
                     LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
                     GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
                     RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
                     PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
                     AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV*"
     CDS             complement(3523..4383)
                     /label="ORF frame 1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     terminator      4488..4641
                     /label="up_PA_terminator"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     terminator      4488..4536
                     /label="synth_PA_terminator"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    4488..4641
                     /label="TB"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    4590..4609
                     /label="RVprimer3_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA TATCAGATCT GCGCAGCACC
   61 ATGGCCTGAA ATAACCTCTG AAAGAGGAAC TTGGTTAGGT ACCTTCTGAG GCGGAAAGAA
  121 CCAGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG
  181 AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC
  241 CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC
  301 CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG
  361 CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA
  421 GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTTG GCAATCCGGT
  481 ACTGTTGGTA AAGCCACCAT GGAAGATGCC AAAAACATTA AGAAGGGCCC AGCGCCATTC
  541 TACCCACTCG AAGACGGGAC CGCCGGCGAG CAGCTGCACA AAGCCATGAA GCGCTACGCC
  601 CTGGTGCCCG GCACCATCGC CTTTACCGAC GCACATATCG AGGTGGACAT TACCTACGCC
  661 GAGTACTTCG AGATGAGCGT TCGGCTGGCA GAAGCTATGA AGCGCTATGG GCTGAATACA
  721 AACCATCGGA TCGTGGTGTG CAGCGAGAAT AGCTTGCAGT TCTTCATGCC CGTGTTGGGT
  781 GCCCTGTTCA TCGGTGTGGC TGTGGCCCCA GCTAACGACA TCTACAACGA GCGCGAGCTG
  841 CTGAACAGCA TGGGCATCAG CCAGCCCACC GTCGTATTCG TGAGCAAGAA AGGGCTGCAA
  901 AAGATCCTCA ACGTGCAAAA GAAGCTACCG ATCATACAAA AGATCATCAT CATGGATAGC
  961 AAGACCGACT ACCAGGGCTT CCAAAGCATG TACACCTTCG TGACTTCCCA TTTGCCACCC
 1021 GGCTTCAACG AGTACGACTT CGTGCCCGAG AGCTTCGACC GGGACAAAAC CATCGCCCTG
 1081 ATCATGAACA GTAGTGGCAG TACCGGATTG CCCAAGGGCG TAGCCCTACC GCACCGCACC
 1141 GCTTGTGTCC GATTCAGTCA TGCCCGCGAC CCCATCTTCG GCAACCAGAT CATCCCCGAC
 1201 ACCGCTATCC TCAGCGTGGT GCCATTTCAC CACGGCTTCG GCATGTTCAC CACGCTGGGC
 1261 TACTTGATCT GCGGCTTTCG GGTCGTGCTC ATGTACCGCT TCGAGGAGGA GCTATTCTTG
 1321 CGCAGCTTGC AAGACTATAA GATTCAATCT GCCCTGCTGG TGCCCACACT ATTTAGCTTC
 1381 TTCGCTAAGA GCACTCTCAT CGACAAGTAC GACCTAAGCA ACTTGCACGA GATCGCCAGC
 1441 GGCGGGGCGC CGCTCAGCAA GGAGGTAGGT GAGGCCGTGG CCAAACGCTT CCACCTACCA
 1501 GGCATCCGCC AGGGCTACGG CCTGACAGAA ACAACCAGCG CCATTCTGAT CACCCCCGAA
 1561 GGGGACGACA AGCCTGGCGC AGTAGGCAAG GTGGTGCCCT TCTTCGAGGC TAAGGTGGTG
 1621 GACTTGGACA CCGGTAAGAC ACTGGGTGTG AACCAGCGCG GCGAGCTGTG CGTCCGTGGC
 1681 CCCATGATCA TGAGCGGCTA CGTTAACAAC CCCGAGGCTA CAAACGCTCT CATCGACAAG
 1741 GACGGCTGGC TGCACAGCGG CGACATCGCC TACTGGGACG AGGACGAGCA CTTCTTCATC
 1801 GTGGACCGGC TGAAGAGCCT GATCAAATAC AAGGGCTACC AGGTAGCCCC AGCCGAACTG
 1861 GAGAGCATCC TGCTGCAACA CCCCAACATC TTCGACGCCG GGGTCGCCGG CCTGCCCGAC
 1921 GACGATGCCG GCGAGCTGCC CGCCGCAGTC GTCGTGCTGG AACACGGTAA AACCATGACC
 1981 GAGAAGGAGA TCGTGGACTA TGTGGCCAGC CAGGTTACAA CCGCCAAGAA GCTGCGCGGT
 2041 GGTGTTGTGT TCGTGGACGA GGTGCCTAAA GGACTGACCG GCAAGTTGGA CGCCCGCAAG
 2101 ATCCGCGAGA TTCTCATTAA GGCCAAGAAG GGCGGCAAGA TCGCCGTGTA ATAATTCTAG
 2161 AGTCGGGGCG GCCGGCCGCT TCGAGCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA
 2221 ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT
 2281 TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT
 2341 ATGTTTCAGG TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA
 2401 TGTGGTAAAA TCGATAAGGA TCCGTCGACC GATGCCCTTG AGAGCCTTCA ACCCAGTCAG
 2461 CTCCTTCCGG TGGGCGCGGG GCATGACTAT CGTCGCCGCA CTTATGACTG TCTTCTTTAT
 2521 CATGCAACTC GTAGGACAGG TGCCGGCAGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC
 2581 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT
 2641 TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG
 2701 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG
 2761 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT
 2821 ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA
 2881 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT
 2941 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC
 3001 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA
 3061 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG
 3121 TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG
 3181 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT
 3241 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA
 3301 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC
 3361 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA
 3421 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA
 3481 CTTGGTCTGA CAGCGGCCGC AAATGCTAAA CCACTGCAGT GGTTACCAGT GCTTGATCAG
 3541 TGAGGCACCG ATCTCAGCGA TCTGCCTATT TCGTTCGTCC ATAGTGGCCT GACTCCCCGT
 3601 CGTGTAGATC ACTACGATTC GTGAGGGCTT ACCATCAGGC CCCAGCGCAG CAATGATGCC
 3661 GCGAGAGCCG CGTTCACCGG CCCCCGATTT GTCAGCAATG AACCAGCCAG CAGGGAGGGC
 3721 CGAGCGAAGA AGTGGTCCTG CTACTTTGTC CGCCTCCATC CAGTCTATGA GCTGCTGTCG
 3781 TGATGCTAGA GTAAGAAGTT CGCCAGTGAG TAGTTTCCGA AGAGTTGTGG CCATTGCTAC
 3841 TGGCATCGTG GTATCACGCT CGTCGTTCGG TATGGCTTCG TTCAACTCTG GTTCCCAGCG
 3901 GTCAAGCCGG GTCACATGAT CACCCATATT ATGAAGAAAT GCAGTCAGCT CCTTAGGGCC
 3961 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC GGTGTTGTCG CTCATGGTAA TGGCAGCACT
 4021 ACACAATTCT CTTACCGTCA TGCCATCCGT AAGATGCTTT TCCGTGACCG GCGAGTACTC
 4081 AACCAAGTCG TTTTGTGAGT AGTGTATACG GCGACCAAGC TGCTCTTGCC CGGCGTCTAT
 4141 ACGGGACAAC ACCGCGCCAC ATAGCAGTAC TTTGAAAGTG CTCATCATCG GGAATCGTTC
 4201 TTCGGGGCGG AAAGACTCAA GGATCTTGCC GCTATTGAGA TCCAGTTCGA TATAGCCCAC
 4261 TCTTGCACCC AGTTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCGG GGTGTGCAAA
 4321 AACAGGCAAG CAAAATGCCG CAAAGAAGGG AATGAGTGCG ACACGAAAAT GTTGGATGCT
 4381 CATACTCGTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTACTAGT ACGTCTCTCA
 4441 AGGATAAGTA AGTAATATTA AGGTACGGGA GGTATTGGAC AGGCCGCAAT AAAATATCTT
 4501 TATTTTCATT ACATCTGTGT GTTGGTTTTT TGTGTGAATC GATAGTACTA ACATACGCTC
 4561 TCCATCAAAA CAAAACGAAA CAAAACAAAC TAGCAAAATA GGCTGTCCCC AGTGCAAGTG
 4621 CAGGTGCCAG AACATTTCTC T
//

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