PCDH-CMV-mCherry-TRIM20

LOCUS       PCDH-CMV-mCherry-TRIM20            9495 bp DNA     circular SYN 18-AUG-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9495)
  AUTHORS   15129015045
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9495
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1798..1915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     1904..1953
                     /label=Primer 4
     enhancer        1938..2241
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2242..2445
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2444..2491)
                     /label=Primer 1
     primer_bind     2466..2500
                     /label=Primer 2
     primer_bind     2466..2492
                     /label=Primer 5
     CDS             2496..3203
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     3025..3046
                     /label=mcherry-F
     misc_feature    3204..3232
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     3220..3258
                     /label=TRIM20-F
     misc_feature    3240..5585
                     /label=TRIM20
     primer_bind     complement(5568..5604)
                     /label=TRIM20-R
     misc_feature    5592..6179
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6063..6074)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6252..6485
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6557..6678
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      6697..6832
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6870..6886)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6894..6910
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6918..6948)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6963..6984
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7272..7860)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8031..8891)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8892..8996)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9365..9378)
                     /label=Primer 5
     primer_bind     9470..9486
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagttgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     1981 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     2041 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     2101 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     2161 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     2221 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     2281 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     2341 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     2401 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
     2461 cagattctag accgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggaggata
     2521 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     2581 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     2641 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     2701 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     2761 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     2821 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     2881 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     2941 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     3001 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     3061 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     3121 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     3181 gcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct tcggaattca
     3241 tggctaagac ccctagtgac catctgctgt ccaccctgga ggagctggtg ccctatgact
     3301 tcgagaagtt caagttcaag ctgcagaaca ccagtgtgca gaaggagcac tccaggatcc
     3361 cccggagcca gatccagaga gccaggccgg tgaagatggc cactctgctg gtcacctact
     3421 atggggaaga gtacgccgtg cagctcaccc tgcaggtcct gcgggccatc aaccagcgcc
     3481 tgctggccga ggagctccac agggcagcca ttcaggaata ttccacacaa gaaaacggca
     3541 cagatgattc cgcagcgtcc agctccctgg gggagaacaa gcccaggagc ctgaagactc
     3601 cagaccaccc cgaggggaac gaggggaacg gccctcggcc gtacgggggc ggagctgcca
     3661 gcctgcggtg cagccagccc gaggccggga gggggctgtc gaggaagccc ctgagcaaac
     3721 gcagagagaa ggcctcggag ggcctggacg cgcagggcaa gcctcggacc cggagcccgg
     3781 ccctgccggg cgggagaagc cccggcccct gcagggcgct agaggggggc caggccgagg
     3841 tccggctgcg cagaaacgcc agctccgcgg ggaggctgca ggggctggcg gggggcgccc
     3901 cggggcagaa ggagtgcagg cccttcgaag tgtacctgcc ctcgggaaag atgcgaccta
     3961 gaagccttga ggtcaccatt tctacagggg agaaggcgcc cgcaaatcca gaaattctcc
     4021 tgactctaga ggaaaagaca gctgcgaatc tggactcggc aacagaaccc cgggcaaggc
     4081 ccactccgga tggaggggca tctgcggacc tgaaggaagg ccctggaaat ccagaacatt
     4141 cggtcaccgg aaggccacca gacacggctg cgagtccccg ctgccacgcc caggaaggag
     4201 acccagttga cggtacctgt gtgcgtgatt cctgcagctt ccccgaggca gtttctgggc
     4261 acccccaggc ctcaggcagc cgctcacctg gctgcccccg gtgccaggac tcccatgaaa
     4321 ggaagagccc gggaagccta agcccccagc ccctgccaca gtgtaagcgc cacctgaagc
     4381 aggtccagct gctcttctgt gaggatcacg atgagcccat ctgcctcatc tgcagtctga
     4441 gtcaggagca ccaaggccac cgggtgcgcc ccattgagga ggtcgccctg gaacacaaga
     4501 agaaaattca gaagcagctg gagcatctga agaagctgag aaaatcaggg gaggagcagc
     4561 gatcctatgg ggaggagaag gcagtgagct ttctgaaaca aactgaagcg ctgaagcagc
     4621 gggtgcagag gaagctggag caggtgtact acttcctgga gcagcaagag catttctttg
     4681 tggcctcact ggaggacgtg ggccagatgg ttgggcagat caggaaggca tatgacaccc
     4741 gcgtatccca ggacatcgcc ctgctcgatg cgctgattgg ggaactggag gccaaggagt
     4801 gccagtcaga atgggaactt ctgcaggaca ttggagacat cttgcacagg gctaagacag
     4861 tgcctgtccc tgaaaagtgg accactcctc aagagataaa acaaaagatc caactcctcc
     4921 accagaagtc agagtttgtg gagaagagca caaagtactt ctcagaaacc ctgcgttcag
     4981 aaatggaaat gttcaatgtt ccggagctga ttggcgctca ggcacatgct gttaatgtga
     5041 ttctggatgc agaaaccgct taccccaacc tcatcttctc tgatgatctg aagagtgtta
     5101 gacttggaaa caagtgggag aggctgcctg atggcccgca aagatttgac agctgtatca
     5161 ttgttctggg ctctccgagt ttcctctctg gccgccgtta ctgggaggtg gaggttggag
     5221 acaagacagc atggatcctg ggagcctgca agacatccat aagcaggaaa gggaacatga
     5281 ctctgtcgcc agagaatggc tactgggtgg tgataatgat gaaggaaaat gagtaccagg
     5341 cgtccagcgt tcccccgacc cgcctgctaa taaaggagcc tcccaagcgt gtgggcatct
     5401 tcgtggacta cagagttgga agcatctcct tttacaatgt gacagccaga tcccacatct
     5461 atacattcgc cagctgctct ttctctgggc cccttcaacc tatcttcagc cctgggacac
     5521 gtgatggagg gaagaacaca gctcctctga ctatctgtcc agtgggtggt caggggcctg
     5581 actgaggatc caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     5641 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     5701 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     5761 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     5821 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     5881 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     5941 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     6001 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     6061 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     6121 ttccgcgtct ccgccttcgc cctcagacga gtcggatctc cctttggccg cctccccgcc
     6181 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     6241 aaagggggga ctggaagggc taattcactc ccaacgaaaa taagatctgc tttttgcttg
     6301 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     6361 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     6421 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     6481 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     6541 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     6601 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     6661 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccagt
     6721 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     6781 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagacttt
     6841 tgcagagacg gcccaaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     6901 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     6961 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7021 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     7081 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7141 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     7201 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7261 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7321 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7381 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     7441 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     7501 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     7561 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     7621 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     7681 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     7741 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     7801 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     7861 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     7921 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     7981 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     8041 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     8101 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     8161 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     8221 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     8281 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     8341 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     8401 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     8461 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     8521 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     8581 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     8641 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     8701 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     8761 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     8821 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     8881 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     8941 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9001 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     9061 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     9121 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     9181 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     9241 gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga
     9301 tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg
     9361 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     9421 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     9481 gccagtgcca agctg
//