pEMTB-PSmOrange

LOCUS       pEMTB-PSmOrange             5544 bp DNA     circular SYN 04-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5544)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jul 7, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..5544
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      271..280
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             280..309
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             313..1115
                     /codon_start=1
                     /label=MAP7
                     /translation="APVRSETAPDSYKVQDKKNASSRPASAISGQNNNHSGNKPDPPPV
                     LRVDDRQRLARERREEREKQLAAREIVWLEREERARQHYEKHLEERKKRLEEQRQKEER
                     RRAAVEEKRRQRLEEDKERHEAVVRRTMERSQKPKQKHNRWSWGGSLHGSPSIHSADPD
                     RRSVSTMNLSKYVDPVISKRLSSSSATLLNSPDRARRLQLSPWESSVVNRLLTPTHSFL
                     ARSKSTAALSGEAASCSPIIMPYKAAHSRNSMDRPKLFVTPPEGS"
     misc_feature    614..619
                     /label=SDS
     misc_feature    625..630
                     /label=SDS
     misc_feature    665..670
                     /label=SDS
     regulatory      1129..1138
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1135..1842
                     /codon_start=1
                     /product="enhanced orange to far-red photoswitchable 
                     monomeric fluorescent protein (Subach et al., 2012)"
                     /label=PSmOrange3
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGTVNGHEFEVEGKGEGHPYEG
                     FQTVKLKVTKGGPLPFAWDILSPLVTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKMRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEVRMRLKLKDGGHYTSEAKTTYKAKKSVLLPGAYIVGIKLDITSHNEDFTIVEQYERS
                     EARHSTGGKDELYK"
     misc_feature    1583..1588
                     /label=SDS
     polyA_signal    1968..2089
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2096..2551)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2578..2682
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2684..3041
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     misc_feature    3008..3013
                     /label=SDS
     CDS             3076..3870
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    4102..4149
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4478..5066
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     enhancer        5241..5544
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 actcagatct cgagctcaag cttcgaattc gccaccatgg agcagaagct catctcagaa
      301 gaagacctcg gcgcgccagt gcgaagcgaa acagcacccg acagctacaa agtgcaagat
      361 aagaaaaatg cctccagccg ccctgcctct gcaatttcag gacaaaataa caaccactca
      421 ggaaataaac cagaccctcc gcctgtgtta cgtgttgatg accggcagcg gctggcccgg
      481 gagcgacgtg aggaacggga gaaacagcta gctgcaagag aaatagtgtg gttagaaaga
      541 gaagagcgag ccaggcagca ctacgagaag cacctggaag agcggaagaa gaggttggag
      601 gagcagaggc agaaggagga gcggaggagg gctgctgtgg aggagaagcg gaggcagaga
      661 cttgaggagg acaaagaacg ccacgaagct gttgtacggc gcacaatgga aaggagccag
      721 aagccaaaac agaagcataa ccgttggtcg tggggaggct ctctccatgg gagccctagc
      781 atccacagtg cagatccaga caggcggtca gtttccacca tgaatctttc gaaatatgtt
      841 gatcccgtca ttagcaagcg gctctcctct tcatctgcaa ctttactaaa ttctccagat
      901 agagctcgcc gcctgcagct cagcccatgg gagagcagcg ttgttaacag actcctgacg
      961 cccacacatt cgttcctggc cagaagtaaa agcacagctg ccttgtctgg agaagcagca
     1021 tcttgcagcc ccatcatcat gccctacaaa gctgcacact ctagaaattc gatggatcga
     1081 ccaaaactct ttgtaacacc acctgagggc tcttcggatc caccggtcgc caccatggtg
     1141 agcaagggcg aggagaataa tatggccatc attaaggagt tcatgcgctt caaggtgcac
     1201 atggagggca ctgtgaacgg ccacgagttc gaggtcgagg gcaagggcga gggccacccc
     1261 tacgagggct ttcagaccgt taagctgaag gtgaccaagg gtggccccct gcccttcgcc
     1321 tgggacatcc tgtcccctct cgtcacctac ggctccaagg cgtacgtgaa gcaccccgcc
     1381 gacatccccg actacttcaa gctgtccttc cccgagggat tcaagtggga gcgcgtgatg
     1441 aactacgagg acggcggcgt ggtgaccgtg acccaggact cctctctgca ggacggcgag
     1501 ttcatctaca aggtgaagat gcgcggcacc aacttcccct ccgacgggcc cgtaatgcag
     1561 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
     1621 aagggcgagg tcaggatgag gctgaagctg aaggatggcg gccactacac ctccgaggcc
     1681 aagactacct acaaggccaa gaagtccgtg ctgctgcccg gcgcctacat cgtcggcatc
     1741 aagttggaca ttacctcaca caacgaggac ttcaccatcg tggaacagta tgaacgcagc
     1801 gaggcccgcc actccaccgg cggcaaggac gagctgtaca agtaaagcgg ccgcgactct
     1861 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     1921 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     1981 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     2041 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt
     2101 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     2161 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     2221 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     2281 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     2341 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     2401 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     2461 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     2521 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
     2581 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     2641 taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc
     2701 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     2761 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     2821 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     2881 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     2941 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     3001 agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga
     3061 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     3121 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     3181 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     3241 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     3301 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     3361 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     3421 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     3481 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     3541 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     3601 gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     3661 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     3721 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     3781 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     3841 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     3901 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     3961 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     4021 tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac
     4081 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt
     4141 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga
     4201 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt
     4261 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag
     4321 gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     4381 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     4441 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     4501 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     4561 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     4621 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     4681 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     4741 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     4801 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     4861 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     4921 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     4981 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     5041 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     5101 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     5161 accgtattac cgccatgcat tagttattaa tagtaatcaa ttacggggtc attagttcat
     5221 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     5281 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     5341 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     5401 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     5461 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     5521 gtattagtca tcgctattac catg
//